LOCUS       sequence-366628-       20402 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta 
            (VPR); ribozymes based sgRNA transcription unit; Terbinafine and 
            Phleomycin selection markers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20402)
  AUTHORS   Mozsik L, Hoekzema M, de Kok NAW, Bovenberg RAL, Nygard Y, Driessen 
            AJM
  TITLE     CRISPR-based transcriptional activation tool for silent genes in 
            filamentous fungi.
  JOURNAL   Sci Rep. 2021 Jan 13;11(1):1118. doi: 10.1038/s41598-020-80864-3.
  PUBMED    33441979
REFERENCE   2  (bases 1 to 20402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-01-13"; volume: "11(1):1118. doi"; pages: " 
            10.1038/s41598-020-80864-3"
COMMENT     Sequence Label: pLM-AMA18.0 dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..20402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(3156..3403)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     3369..3387
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(3488..3862)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(4654..4674)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4654..4672)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4693..4709)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4717..4733
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4741..4747,4748..4765,4766..4771))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4786..4807
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4924..4941)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5095..5683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5175..5194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6072..6731)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEXRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     6646..6665
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(6732..6834)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     promoter        complement(7154..7258)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7284..7739)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7421..7442)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7633..7652
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7881..7897
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7907..7926
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7907..7925
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          8448..8563
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     protein_bind    8635..8656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8671..8676,8677..8694,8695..8701)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8709..8725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8733..8749
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             8745..9068
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    8762..8818
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(8819..8835)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9044..9063)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_RNA        9162..9237
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           9242..9309
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     intron          11413..11528
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             complement(12700..13269)
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     CDS             complement(13288..13644)
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             complement(14074..14094)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(14119..14268)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(14293..14313)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(14326..15840,15841..15843,15844..15909,
                     15910..15915,15916..18399,18400..18402,18403..18429))
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gtcttggact agtcccgagc aaaaaggatt ggaggaggag gaggaaggtg agagtgagac
       61 aaagagcgaa ataagagctt caaaggctat ctctaagcag tatgaaggtt aagtatctag
      121 ttcttgacta gatttaaaag agatttcgac tagttatgta cctggagttt ggatatagga
      181 atgtgttgtg gtaacgaaat gtaaggggga ggaaagaaaa agtcggtcaa gaggtaactc
      241 taagtcggcc attccttttt gggaggcgct aaccataaac ggcatggtcg acttagagtt
      301 agctcaggga atttagggag ttatctgcga ccaccgagga acggcggaat gccaaagaat
      361 cccgatggag ctctagctgg cggttgacaa ccccaccttt tggcgtttct gcggcgttgc
      421 aggcgggact ggatacttcg tagaaccaga aaggcaaggc agaacgcgct cagcaagagt
      481 gttggaagtg atagcatgat gtgccttgtt aactaggtca aaatctgcag tatgcttgat
      541 gttatccaaa gtgtgagaga ggaaggtcca aacatacacg attgggagag ggcctaggta
      601 taagagtttt tgagtagaac gcatgtgagc ccagccatct cgaggagatt aaacacgggc
      661 cggcatttga tggctatgtt agtaccccaa tggaaagcct gagagtccag tggtcgcaga
      721 taactcccta aattccctga gctaactcta agtcgaccat gccgtttatg gttagcgcct
      781 cccaaaaagg aatggccgac ttagagttac ctcttgaccg actttttctt tcctccccct
      841 tacatttcgt taccacaaca cattcctata tccaaactcc aggtacataa ctagtcgaaa
      901 tctcttttaa atctagtcaa gaactagata cttaaccttc atactgctta gagatagcct
      961 ttgaagctct tatttcgctc tttgtctcac tctcaccttc ctcctcctcc tccaatcctt
     1021 tttgctcggg actagtccaa gactagtcaa cgattagtat gccgagcatt cgcgataacg
     1081 atctccgaaa gtccccagag tactgtcact atctcgaggc agttaaggac ggggagctta
     1141 cgctgccgga tttcaagata gtaagccgac ccgactagct ttaagcctag ttactgacta
     1201 gtccacagga cgacaatggc gtgcctgata tccatccata tgaagtctac tgccgagtga
     1261 agggatgcct caagcgtaca gtgagtctac tgctatacta gtttctgact agttattaac
     1321 tagttgacag gttccctctg ccaacagaaa catattggtc aagcacttga aggacaagaa
     1381 ctcccacggc atggagttta cattgcacaa tggacctccc actatgaagg aactgatgga
     1441 ggccaaaggc aagtcctatc tagattactt ggtgactagt ccctgactag tcaagtagca
     1501 tggtatgaag gcttgtttga aggcactgtt ctcccaaccc cgactcctac caagaagcgc
     1561 aagcgagctg cgtaagtttc tgtgagtcta actagtgtat tagctaatat atagcagcac
     1621 caagtccaag gaccacaata ctaagggtgt cgagaattcg tgagtttctt ctcccatttc
     1681 aactagtcct tgactagtca ctaactactt tgcagcaacg agggtgaagc tggaaatgat
     1741 caggacaatg gcgagggccc gtaagtacag ccattcaatg cagactagtt gctaactaat
     1801 ctgtgactag ttcaagtggt ccgtacgccg tgcatacccc tgtgactggt aggaatttga
     1861 gcaagcctgt cttgccgcgc gatgagaaag gaaaggcaag ttacattcag ccccgtacct
     1921 aggatcagtg ctaatttata acctctagcc actctttatg cagatccgcc gtgagggtag
     1981 caaggcagct aaatcagctg gtgagaaagg aaccataccc tgcaagacct gtcgcaacgc
     2041 aaagggcaaa ggtaagctat ccaagctagt ttgggactag attctaacta gtctcagcac
     2101 cgtgtggttc aaagccatat tgcgagtttt ggcgcttttt ctcatcgatt gacgaggcaa
     2161 agggagcgag tatgcaacct cgtaagtcag actcagacaa agaccaacta gttattgacc
     2221 agtcttctag aaggctctgt tgtggatctt gaggccctgg agagttcctc caacaatccg
     2281 gagacaagca agtcctcgtc ggactagtca ctaactagac tctaactagt tgcagacatg
     2341 gataatgcaa aagagacaag caatgaagaa agtggtaaga catttctcct ttgtggttct
     2401 ggactagtct ttgactagtc acagtcttaa acaaggaaaa tgagcatgaa aatgaggagg
     2461 aaaaggctgc tgagcccgag gaagtgcagg gtgatggcag acatggtagg ttaatacctt
     2521 gttagttatt gctagtcact gactagtcaa taactagtct ctgaacacct tgcaatcact
     2581 ccgtttgcgc agctgaacag tggtgaggat aatagtagta agttattcta gcttcagagt
     2641 tataggagac tagatactaa ctagtattag ttgcaactaa cctggatctc agagactttg
     2701 gcctcaatct agaatctatc tagttgtcaa ctagactgtg gtatcattgt cttttatttt
     2761 cctagtcctg gaactagctt ctaactagtc tccctaatat gtggctgtct tgtttttttt
     2821 ttttgtttcc ctacccggat atctagtccc cttctaggtt ctgttaacct ctcgggctct
     2881 gatttagttt aacgcaaacc tgagattagt ttctaactag tctctaggtt ttctatccac
     2941 ctttaattgt aataataaat acaagcaacg tttatacgtc aaaagcattt ataaactttt
     3001 accctaaagt agcttgcttg tgtgtttagt ttataattag tctcttatta atttgatgta
     3061 ggtaagcccg ccacaaatat atatttttaa caagataccg tggaaaaact tcgtgctatc
     3121 acaaaacagt atacaaaaaa taagctatca agcttgcaaa ttaaagcctt cgagcgtccc
     3181 aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg tctgtacaga
     3241 aaaaaaagaa aaatttgaaa tataaataac gttcttaata ctaacataac tataaaaaaa
     3301 taaataggga cctagacttc aggttgtcta actccttcct tttcggttag agcggatgtg
     3361 gggggagggc gtgaatgtaa gcgtgacata actaattaca tgatatcgac aaaggaaaag
     3421 ggggacggat ctccgaggcc tcggacccgt cgggccgcgt cggaccggcg gtgttggtcg
     3481 gcgtcggtca gtcctgctcc tcggccacga agtgcacgca gttgccggcc gggtcgcgca
     3541 gggcgaactc ccgcccccac ggctgctcgc cgatctcggt catggccggc ccggaggcgt
     3601 cccggaagtt cgtggacacg acctccgacc actcggcgta cagctcgtcc aggccgcgca
     3661 cccacaccca ggccagggtg ttgtccggca ccacctggtc ctggaccgcg ctgatgaaca
     3721 gggtcacgtc gtcccggacc acaccggcga agtcgtcctc cacgaagtcc cgggagaacc
     3781 cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc gcgcgcggtg agcaccggaa
     3841 cggcactggt caacttggcc atggtgtcta gaaaaataat ggtgaaaact tgaaggcgtg
     3901 gacaacgacg aagagagggc ttggcaaagg gacaggggaa gttctcctgc agatgacaat
     3961 gagtgaagag tatggacgca atggaaacga cctgagctct cttcctgtgt gcctgagcgg
     4021 gggcggggtg tttatgctga gacaacctgg agacattata cgcttgcagc ccagatgctt
     4081 actggatggg gccgctggag ccagtgtaaa attagtaacc gtatctcgaa gtcggagggt
     4141 ctttggtggt ctgagatttc agtcggtccg caccgtggca tttgcagacg gtgcgatcag
     4201 gccaatcgtt gatgctcggg cagagcaaca ctcccccgct cgaagactag taagtactta
     4261 tcattaccgt gccagaaaac ggggccatag atacccaagt aacaccgtcg agtcaatcgg
     4321 gctcgtgggc ccagccaagc cacgagagag taggcaacgt gcactcaacg acggcgatgt
     4381 tccaaggtaa accggcacgt agaaaatgtc cggaccacct tggctctcgt tgcagcgtgt
     4441 tgaatcttca gccaccgtaa gtcgatagca tccggttaga gtgcaacgtg ggtctgtctc
     4501 attcttctcg gttcttggca ccagaatcgg gcgtagtttg cccactgcca agtcgcgggg
     4561 ccgcttggct gtccctgtgg tgggatttcc cgatgcaaca tgcagataca tgtagtcgac
     4621 ctcgaggggg ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     4681 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4741 caacatacga gccgggagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4801 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4861 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4921 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4981 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     5041 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     5101 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     5161 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     5221 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     5281 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     5341 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5401 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5461 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5521 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5581 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5641 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5701 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5761 attatcaaaa aggatcttca cctagatcct ttttgatcag aaaaaaagga tcatatcgtc
     5821 aattattacc tccacgggga gagcctgagc aaactggcct caggcatttg agaagcacac
     5881 ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat
     5941 ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc
     6001 atccgcttat tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg
     6061 ccttaaaaaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     6121 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     6181 agcaccttgt cgccttgcgt ataatatttg cccatrgtga aaacgggggc gaagaagttg
     6241 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
     6301 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     6361 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     6421 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     6481 atcaccagct caccgtcttt cattgccata cgkaaytccg gatgagcatt catcaggcgg
     6541 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     6601 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     6661 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     6721 tttttctcca ttttagcttc cttagctcct gaaaatctcg ayaactcaaa aaatacgccc
     6781 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct
     6841 cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt
     6901 tattctgcga agtgatcttc cgtcacaggt atttattcgg tcgaaaagtg ctcatcattg
     6961 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7021 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7081 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7141 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7201 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7261 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     7321 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     7381 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     7441 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     7501 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     7561 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     7621 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     7681 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     7741 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     7801 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     7861 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     7921 atagggcgaa ttggagctcc accgcggtgg cggccatcac gcgttaccct gcaccgtacc
     7981 tactaccttc aatatcaact ctttcagggg gggagcggcc ttaagtcggc aacgagaggt
     8041 atgtctaaag tctccgcatg cgaattctct gagcggcgta agctccctaa ttggcccatc
     8101 cggcatctgt agggcgtcca aatatcgtgc ctctcctgct ttgcccggtg tatgaaaccg
     8161 gaaaggccgc tcaggagctg gccagcggcg cagaccggga acacaagctg gcagtcgacc
     8221 catccggtgc tctacactcg acctgctgag gtccctcagt ccctggtagg cagctttgcc
     8281 ccgtctgtcc gcccggtgtg tcggcggggt tgacaaggtc gttgcgtcag tccaacattt
     8341 gttgccatat tttcctgctc tccccaccag ctgctctttt cttttctctt tcttttccca
     8401 tcttcagtat attcatcttc ccatccaaga acctttattt cccctaagta agtactttgc
     8461 tacatccata ctccatcctt cccatccctt attcctttga acctttcagt tcgagctttc
     8521 ccacttcatc gcagcttgac taacagctac cccgcttgag cagacatcac cgacgagacc
     8581 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     8641 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     8701 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     8761 caagcttgca tgcctgcagg tcgactctag aggatccccg ggtaccgagc tcgaattcac
     8821 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     8881 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     8941 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
     9001 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
     9061 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     9121 gtctgctccc ggcatccgct tacagacaag ctgtggtctc cgttttagag ctagaaatag
     9181 caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     9241 tggccggcat ggtcccagcc tcctcgctgg cgccggctgg gcaacatgct tcggcatggc
     9301 gaatgggact gatttaatag ctccatgtca acaagaataa aacgcgtttc gggtttacct
     9361 cttccagata cagctcatct gcaatgcatt aatgcattgg acctcgcaac cctagtacgc
     9421 ccttcaggct ccggcgaagc agaagaatag cttagcagag tctattttca ttttcgggag
     9481 acgagatcaa gcagatcaac ggtcgtcaag agacgtacga gactgaggaa tccgctcttg
     9541 gctcctcccc atgggaattc gtaaagcgta ccgctcgtac catgggttga gtggtatggg
     9601 gccatccaga gtcacctgtg gcagcatgag actgcactcg aagcagccat caacccagcc
     9661 aatattctgg gctttccatc cttagatcac atttgagata taacccattt ggtgagagac
     9721 acttgtgccg ttatacgtgt ctagactgga aacgcaaccc tgaagggatt cttcctttga
     9781 gagatggaag cgtgtcatat ctcttcggtt ctacggcagg tttttttctg ctctttcgta
     9841 gcatggcatg gtcacttcag cgcttattta cagttgctgg tattgatttc ttgtgcaaat
     9901 tgctatctga cacttattag aagctcagta ccagatctcg gtccagatgt atggcaaaat
     9961 cacgacagag gctgtgtaca atatcatgac ggcgtagtat ggggctagga atagcttgga
    10021 cagaggggta tcgcatatgt ggacccagat ggcgaggaag gcaacggaga agaagtggct
    10081 gacaaggacg aggggtttct tgattagacc ggctaacaac ccgacagggc cgccaactgg
    10141 tccaatctgg aagtagcgga aacagccacg ctgaagagct ttgaggtaat agtctgttga
    10201 atgaacagtt agttattgca aattaacctg aagagtgagc gtgcgtactg tctgcagcga
    10261 agagcgcata gagagcctgt gcgaggatat tgatgaccga agatgagttc tttcgtttcc
    10321 agtggaactc tgcgagctgc tcgagaacaa ggcctgtatt ggagaggttg ggcacgcgct
    10381 ctgggctgag caattcgcgg atgacacaaa catcgttgag cgccacagtc atgcctccac
    10441 ctgtgagagg gtgccgcatg ttaagcgcat cgcccagaat catcaagccg ggtgtcttgt
    10501 tggccgaggc aggcaggaac gagttcggca ttgaccgtag ctggcccttc tctagggcat
    10561 cggagaacga ctgctggaca ccctctggga gcttgggcag gatgttgttt cgcatgtaac
    10621 ccttgacacc accgttcttg accgaggccg caggcaggtt atcagggata tcgataagga
    10681 ttcgggtttc atgggtgccg atctggtaca taagtacggg tggattatcg ctgagaagca
    10741 catgaccagt gttgggagcg ggcagtttag catcgatgag ctccaagcca tggaactttg
    10801 agcgccgttt gggtgtatac tggtggtgtt ccttgcggaa aacggagtta taaccgtcgg
    10861 cggccacggt gagttgaccg aagtagcagt ccttgacatc cttggttata cactcgactc
    10921 caaggacttc ctgagtatat gaggatgtga tgaggccggt aaccttggtt tcgaccaccg
    10981 tgacattggg gcacgacaga gcggctgccc gtagtttcat cacgaatcgg ccatggtgga
    11041 aggctcgtcc caacggtgta ggcgaggttg ggctggactt ggggtattcg aggagaaccg
    11101 gctccccaaa gtaagtaacc cagtatccct tcacattgat gccatcgata tcttccagac
    11161 aatcccgtag ccctagttgc tcgagcgcct gaacacctcc cggttggagt agttcaccaa
    11221 caatacggtt gggctcttcc aacgacttct ccagcaggat tacactgcgc ccttgatttc
    11281 ccagtgcaac tgcaagtgca cagccgagga ctccggcacc cacaattaca atatcagctt
    11341 catggtgttc gatgcggcgt tgagcggccg gggagaggga ggcgtggcca ttgagcaagg
    11401 tcattgtgat gtctgctcaa gcggggtagc tgttagtcaa gctgcgatga agtgggaaag
    11461 ctcgaactga aaggttcaaa ggaataaggg atgggaagga tggagtatgg atgtagcaaa
    11521 gtacttactt aggggaaata aaggttcttg gatgggaaga tgaatatact gaagatggga
    11581 aaagaaagag aaaagaaaag agcagctggt ggggagagca ggaaaatatg gcaacaaatg
    11641 ttggactgac gcaacgacct tgtcaacccc gccgacacac cgggcggaca gacggggcaa
    11701 agctgcctac cagggactga gggacctcag caggtcgagt gcagagcacc ggatgggtcg
    11761 actgccagct tgtgttcccg gtctgcgccg ctggccagct cctgagcggc ctttccggtt
    11821 tcatacaccg ggcaaagcag gagaggcacg atatttggac gccctacaga tgccggatgg
    11881 gccaattagg gagcttacgc gccgggtact cgctctacct acttcggaga aggtactatc
    11941 tcgtgaatct tttaccagat cggaagcaat tggacttctg tacctaggtt aatggcatgc
    12001 tatttcgccg acggctatac acccctggct tcacattctc cttcgcttac tgccggtgat
    12061 tcgatgaagc tccatattct ccgatgatgc aatagattct tggtcaacga ggggcacacc
    12121 agcctttcca cttcggggcg gaggggcggc cggtcccgga ttaataatca tccactgcac
    12181 ctcagagccg ccagagctgt ctggcgcagt ggcgcttatt actcagccct tctctctgcg
    12241 tccgtccgtc tctccgcatg ccagaaagag tcaccggtca ctgtacagag ctcgaattcc
    12301 tgcagcccgg gggatccact ccgagctcga attctagtag cggtgcttgg gatgttccat
    12361 ggtagctgtg aagcaattat agaacaacta cttcgagaag aaccttggaa ggattatcaa
    12421 aaacgcaaat tgacactcct accgtggatt tccagaaggt acggccgtga agcaacaaat
    12481 aaaaccatgt ataaagttca aaatttctct aaaccattgc ttttgtatag actgacgaat
    12541 gcaagtaact gacatgctgg atgtcttttt gggaatcatt atcaaccagg tcctacagta
    12601 caaggaaaag agcttcggcc caaagatagg cccgcctgag aagaaggaaa cgttccattc
    12661 aaaaagtcgt aagagagaac cgataaagaa gtaagctcaa aacagagatg tgtcgaagat
    12721 ggacagtcct gtgctgatat gcatggcatg caagaggcac tcgtcgttca ggaaggtatc
    12781 cagaatctcg ttcaattccg gggtcagggg tgagtccagg ttcagatcct cggtcatgga
    12841 ctcaagtgtg gttgtcagct catccagatg gccccttggg ggcggatggg aaaggtccat
    12901 ttggccacag attgcagcct cttccttctg gggaatcaca gtatcggcca tctcccgaag
    12961 ggctttgaca gcctggctcg tctcttcatc gggatcctcc aacaggtgac tggcctcggg
    13021 agtcactgcg ggcgctggat ccagtggctg agggactggt gccggggtca gtgacccgac
    13081 tggctcatgt actggaccgg ttggtgttgg tgcgaggctg gcggggagtg ggcggttggc
    13141 ccatggactt cctggaggat gaaatggccg gattcgtttt ggctggcaca cctcgcggcc
    13201 ctcaaacacg tcactaatag cggagccggc ctcaggcttc ggcaaaaaca tcccttccct
    13261 ggaatcccga gatcccgatc cgctacccag cagtgcggag aagtccatgt cggcgataga
    13321 gctgaagtcc tcgtcgccag acagcagtcc attaggcagg cctggtgctc ccagaggggc
    13381 aggagctgga tcaggaggcc tctgagcgcc tgtcacgagc cgggtgatgg cctcggggta
    13441 ttccatcagc atgggctcgg tggtgtgagg ggccacaggg atgccctggt tcagcagctg
    13501 ctggaactcg ctgttgtcca cgctggccag gtcggtgaac acggcaggat cggtgctgtt
    13561 tcccagcagg gctcccagat cctcgtcgtc gaactgcagc tgcagcagag cttcagacag
    13621 tgtgccctcg ccggcctgtg taggtttagg ggctggtgga gccacagcct gtggaggtcc
    13681 aggagccagc acaggcacgg gtgctggtgc ctgagccagt gcagacacca tggctggagc
    13741 tggtgcagga gcaggagcct gaggcagcac ctgaggaggg gctggagcca gagcagaggc
    13801 ctgagagatc tggccgctgg ggaacaccat ggtagggaac tcgtcgtagt tgatggtgct
    13861 caggctgctg gtgaaggggt aaggctgggg ggcaggtttt ggcacgctgg cgctggatct
    13921 gctgggcacg gcgattcttc taggtggagg tctagggtcg gtggggccgc tgaaggggga
    13981 cttcttcatg atgctcttga atgtctcgta ggtccgcttc cgcttttcct cgatccggtg
    14041 ccggtcgtcg gtgtcgggca ggtactggct accaactttg cgtttctttt tcggagatcc
    14101 ggaacttcta gagttaatca gcatgtccag gtcgaaatca tcaagggcgt cactgccgag
    14161 catgtcgagg tcaaagtcat caagggcatc cgaaccaagc atgtcaaggt caaaatcatc
    14221 gagggcgtca cttcccagca tatccagatc aaaatcgtcc aatgcgtcag cccgtccgga
    14281 accgctggcc tccaccttcc tcttcttctt ggggtcagcc ctgctatccc ctccgagctg
    14341 tgagaggtca atccgagttt cgtaaagtcc ggtgatgctc tggtggatga gggtggcgtc
    14401 cagcacttct ttggtggagg tatagcgttt ccggtcgatg gtggtgtcga agtacttgaa
    14461 tgcggcaggg gcaccaagat tggtgagggt gaacaggtgg atgatattct cagcctgctc
    14521 ccgaattggt ttgtctctgt gcttattgta tgcgctaagg actttatcga ggttggcatc
    14581 agcgagaatg acgcgtttag aaaactcgga aatctgctca atgatctcgt ccagataatg
    14641 cttatgctgc tccacaaaca gttgcttttg ctcgttgtcc tcggggctcc ccttcagctt
    14701 ctcgtaatgg cttgcaaggt agaggaaatt cacgtacttg gaggggagag ccagctcatt
    14761 acccttctga agttcgccag cggaggcgag catccgcttc cgcccattct ccagttcgaa
    14821 caggctatac tttgggagct tgatgataag gtccttcttg acttccttgt aacctttggc
    14881 ttcgagaaag tcaattgggt tcttctcgaa ggagctgcgc tccataatgg tgatacccag
    14941 cagttccttc acgctcttga gcttcttaga ctttcctttt tccaccttag ccacgaccag
    15001 gacagaataa gccacagttg gtgaatcgaa tccgccgtac ttcttagggt cccaatcctt
    15061 cttccttgca atgagcttgt cggagttgcg cttggggagg attgattcct tagaaaagcc
    15121 tccggtttgc acctcggttt tcttcacgat gttcacttga ggcatagaaa gcactttgcg
    15181 cacggtagcg aaatctctgc ccttgtccca cacgatttcg ccagtctccc cattagtctc
    15241 gatgagcggc ctcttccgga tctcaccgtt tgcaagggtg atttcggtct tgaagaaatt
    15301 catgatgttg ctgtagaaga agtactttgc ggtggccttt ccgatctcct gctcagattt
    15361 ggcaatcatc ttgcgcacgt cgtagacctt gtagtctccg tacacgaact cgctttccag
    15421 ctttgggtac ttcttgatca gggcggttcc caccacggca ttcagatatg cgtcatgagc
    15481 atgatgatag ttattgatct cccgcacttt gtagaattga aagtcctttc tgaaatcgct
    15541 gaccagcttg gacttcaggg taatcacctt cacctcgcgg ataagtttgt cgttttcgtc
    15601 gtacttggta ttcatgcgtg agtcaaggat ttgggcgaca tgtttagtaa tctgccgagt
    15661 ctccacaagt tgccgtttaa tgaatcctgc tttgtccagt tcgctcagtc cgcccctctc
    15721 agccttagtg agattgtcga acttccgctg agtgatcagc tttgcgttca gaagctggcg
    15781 ccaatagttc ttcatctttt tcacgacttc ctctgagggc acgttatctg actttccccg
    15841 ggccttgtct gagcgagtca gcaccttatt gtcaattgag tcgtctttga ggaagctctg
    15901 agggacgatg gcagccacgt cgtagtctga gagcctgttg atatcaagct cttggtccac
    15961 atacatatcg cgtccatttt gaaggtagta cagatagagc ttctcattct gaagctgggt
    16021 gttttccacc gggtgctctt taaggatctg tgacccgagc tccttaatcc cttcctcaat
    16081 tcttttcatc cgctccctgc tgttcttctg tcccttctgg gtagtctggt tttccctggc
    16141 catctcgatg acaatgttct ctggtttatg gcgacccatc accttgacca gctcgtccac
    16201 gactttcacg gtctggagaa tacccttctt aatagcgggt gaaccagcca gatttgcgat
    16261 atgctcgtgc agtgagtcac cttgtccaga cacttgggcc ttctggatgt cctccttaaa
    16321 ggtcagggaa tcatcatgga taagttgcat gaagttgcga ttagcgaatc cgtcgctctt
    16381 aaggaaatca agaatggtct ttccgctctg tttgtcccgg attccgttga tgagtttgcg
    16441 gctaaggcgt ccccatccgg tatatcttct ccgtttaagt tgcttcatca ctttatcgtc
    16501 gaacagatgg gcgtaggtct taagcctctc ctcgatcatt tcccggtcct cgaacagagt
    16561 gagagtcagg acaatgtcct caaggatgtc ctcgttctcc tcattgtcca gaaaatcctt
    16621 gtccttgatg atcttaagca gatcatggta agtacccagg cttgcgttga accgatcttc
    16681 gaccccgcta atctccacag agtcgaagca ttcaattttc ttgaaatagt cctccttcag
    16741 ctgcttcacg gtcacctttc tattggtctt gaacagaagg tccacaattg ccttcttttg
    16801 ttcgcctgac aggaatgcgg gcttcctcat cccttcggtg acatatttca ccttggtcag
    16861 ctcgttgtac acggtaaagt actcgtagag cagggaatgt ttcggcagga ccttctcatt
    16921 ggggaggttc ttatcgaagt tagtcatccg ttcgatgaat gactgagcgc tggctccctt
    16981 gtccaccacc tcttcgaagt tccagggagt gatagtttct tctgactttc tggtcatcca
    17041 agcaaaccgg ctatttcctc tggcgagggg tcccacgtag taggggatgc ggaaagtgag
    17101 aattttctca atcttctccc tgttgtcctt aagaaagggg tagaagtcct cttggcgccg
    17161 aaggatggcg tgaagctccc caagatggat ctggtgggga atgctcccat tgtcgaaagt
    17221 gcgctgcttc cgcagcagat cctcccgatt cagtttcacg aggagctctt cggtgccgtc
    17281 catcttctca aggataggtt tgataaactt ataaaattcc tcttgggagg cacctccgtc
    17341 gatgtagccg gcgtatccat tctttgactg gtcaaagaag atttccttgt acttctccgg
    17401 aagctgctgc cggacgagtg ccttaagcag ggtcaggtcc tggtggtgct catcataccg
    17461 cttaatcatt gaagctgaaa gaggggcctt ggtgatttca gtgttcacgc ggagaatgtc
    17521 agacagcagg atggcgtcag aaagattctt agcagcaagg aaaaggtcgg cgtattggtc
    17581 accgatctgg gccaggaggt tgtcgagatc gtcatcatag gtgtccttgg acagttgcag
    17641 cttggcatcc tctgccaggt cgaaattaga cttgaaattg ggagtcagcc caagtgaaag
    17701 agcaatcagg ttcccgaaca gtccattttt cttttcaccc ggcagctgag cgataagatt
    17761 ctcaagtctg cggctctttg acagccgggc tgacagaatg gccttggcat cgactccgct
    17821 tgcattgatg gggttctctt caaagagctg gttgtaagtc tgcaccagct ggatgaacag
    17881 tttatccaca tcgctattgt caggattgag gtcgccctcg atcaggaagt ggcctctgaa
    17941 cttgatcatg tgggccaggg ccaggtagat aagccggagg tctgccttgt cggttgagtc
    18001 caccagcttt ttgcgaagat ggtagatggt tgggtatttc tcgtgatagg ccacctcgtc
    18061 gacgatatta ccgaagatgg gatgccgttc atgcttctta tcctcttcca cgaggaatga
    18121 ttcttccagc ctatggaaga agctgtcgtc gacctttgcc atttcgttgg agaatatctc
    18181 ttgaaggtaa caaatccggt tcttcctcct ggtgtaccgc cgtctagcag tccgcttgag
    18241 gcgggttgct tcagcggttt cccctgaatc aaagaggaga gctccgatca gatttttctt
    18301 gatggaatgc cggtcggtgt tcccgagcac cttgaacttc ttagagggca ccttgtactc
    18361 gtcagtgatc acggcccacc cgacgctatt agtcccgatg gccagtccga tagaatactt
    18421 cttgtccatt gtttgctgtc tatgtggggg actgtccagg gataattgat tagcacgagt
    18481 cgaaacaagc gaagctgatc atctaatgtc cgaatgcctt caattctatc ccgctgagtg
    18541 gtatcgatgg ccgtgagtct gtcggtaacc tttcgctgga tatccaattc gcagtttccc
    18601 agccgcattc agaaaagaat ctttcaagga agttgttcca agcaaaaaaa gtagcaacat
    18661 cgagagttct gcctctgctc ccctgtcgta atgctccatt ccctcttgta tcgataccgg
    18721 gtgaacgagt ggacggatgg aaatttgaca gaccgatcga aggcgcaccg actcgctcga
    18781 caagatagaa ccagcggaaa ccggggggga aaaagatcac aatagccaga aaataaaata
    18841 ctcacctcta atcctgcagc ccttcaccaa gaacgcagcg gcaagggttg tcgtgggttg
    18901 tcgtggagtt gagtgcttgt cgtcggcgat cgtggaatcc gtgtgtgtct ctcgcttcga
    18961 cgttgcccta tttcgtgggc gctagccgaa gccggctagg ccagtacccc gtgacctcgt
    19021 ctacgcttgt cctttcgtgg ttagcttact gacagaccca ggaggctgac ctcgattgtt
    19081 attccgctcc ggacccccac taatttacac caatcacaag tctcgactct agactccaga
    19141 catgtttcta agaactacaa gtttacagca gctcactatc tagcaaagaa cataactaat
    19201 tgcaagatga cgttgtacct cagttgacta acagccagcc aagctattgt cattcgaagt
    19261 tcgagtttcg ttgtacttca catttttgag gtactaacat aacctccgga gtataactct
    19321 caattcttat atcaggtatc ctccacagac tccttgagcc gtgaaactta gcctctatga
    19381 cacttagcaa tcggttgtaa tgccttcact ctacattcgt gtagctatcg cacctctgtg
    19441 ctggtaaacc tgcggcttcg tatagagtca atacaattcc ggctaccctg tatataagaa
    19501 gggtgatgta ccagattgct ttggtgtcga tcggacgtat tgtccaaggg ttttttgctt
    19561 atatatggcc attctaatcg tgatttgact ggacttgctg tactccacgc atttggactg
    19621 gttccggcgc ctgttcagga ttctagttga gactacgtga ttagagctaa tagattgaac
    19681 gagttcaggc ctttctcagt ttccagacta aggagactaa ggaatcggga agtcgaaata
    19741 tctaatctct ccttacctat gccggaccgt ctataactcc tccaagcttg aattcttgca
    19801 gcgttaagac tgaagacagg atgatctggg gctgcaggaa ttcgatagct tattttttgt
    19861 atactgtttt gtgatagcac gaagtttttc cacggtatct tgttaaaaat atatatttgt
    19921 ggcgggctta cctacatcaa attaataaga gactaattat aaactaaaca cacaagcaag
    19981 ctactttagg gtaaaagttt ataaatgctt ttgacgtata aacgttgctt gtatttatta
    20041 ttacaattaa aggtggatag aaaacctaga gactagttag aaactaatct caggtttgcg
    20101 ttaaactaaa tcagagcccg agaggttaac agaacctaga aggggactag atatccgggt
    20161 agggaaacaa aaaaaaaaan caagacagcc acatattagg gagactagtt agaagctagt
    20221 tccaggacta ggaaaataaa agacaatgat accacagtct agttgacaac tagatagatt
    20281 ctagattgag gccaaagtct ctgagatcca ggttagttgc aactaatact agttagtatc
    20341 tagtctccta taactctgaa gctagaataa cttactacta ttatcctcac cactgttcag
    20401 cc
//
