LOCUS       sequence-367135-        5577 bp    DNA     circular SYN 11-DEC-2025
DEFINITION  NDUFA5 Complex I sub-unit with C-terminus mVenus tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Balderas E, Eberhardt DR, Lee S, Pleinis JM, Sommakia S, Balynas AM,
            Yin X, Parker MC, Maguire CT, Cho S, Szulik MW, Bakhtina A, Bia RD, 
            Friederich MW, Locke TM, Van Hove JLK, Drakos SG, Sancak Y, 
            Tristani-Firouzi M, Franklin S, Rodan AR, Chaudhuri D
  TITLE     Mitochondrial calcium uniporter stabilization preserves energetic 
            homeostasis during Complex I impairment.
  JOURNAL   Nat Commun. 2022 May 19;13(1):2769. doi: 10.1038/s41467-022-30236-4.
  PUBMED    35589699
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-19"; volume: "13(1):2769. doi"; pages: " 
            10.1038/s41467-022-30236-4"
COMMENT     Sequence Label: pEGFP-NDUFA5-mVenus
FEATURES             Location/Qualifiers
     source          1..5577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..64
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(145..162)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(316..904)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(396..415)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1075..1866,1867..1935))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1698..1717
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1936..2027)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      complement(2046..2073)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2165..2251)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2404..2421
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     CDS             complement(4054..4071)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(4114..4398)
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(4172..4191)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             4425..5303
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     primer_bind     complement(5459..5481)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    join(5567..5577,1..130)
                     /label=bom
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat
       61 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
      121 ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      181 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      241 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      301 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      361 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      421 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      481 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      541 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      601 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      661 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      721 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
      781 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      841 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      901 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
      961 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1021 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     1081 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1141 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1201 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1261 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1321 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1381 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1441 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1501 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1561 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1621 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1681 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     1741 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     1801 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     1861 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     1921 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     1981 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaaaga gtttgtagaa
     2041 acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg gcagtttatg
     2101 gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa tccgctcccg
     2161 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     2221 agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac tctcgcatgg
     2281 ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca
     2341 ggtgggacca ccgcgctact gccgccaggc aaattctgtt ttatcagacc gcttctgcgt
     2401 tctgatttaa tctgtatcag gctgaaaatc ttctctcatc caccaaaaca gccaagcttc
     2461 gaattctcac tcttccatca cgccgatctg agtggccagc ctctcggcga ccctcttgca
     2521 ggcttggcgg ccatcgagat cgacgtagta cggcgttcgg tgatggcaaa cgatcaatcc
     2581 ccacagtcgc tcgccgcgca aaatcgagat cgacatcgtg ccgtgcatgc ctatgttgcg
     2641 catgaactcc agatggttgg gcgagacgct gcgcaggatg gcgaagctaa gatcaatcgg
     2701 ccgcccggtg accggattga ggtctggggt gaccggcacc ggccgataat tgatgtcggg
     2761 aatgatccgt accgggttga tggtatagag ccgacgggcc tgcgccggga tgaatgaggc
     2821 aggatagtgc aggcctagtt ttgactcgac ctcggcgcac cgatcctctg cgatcacgga
     2881 cccgctgaag tcggaggcga agcgatagat catcacccga tcgaagccgg taatcttccg
     2941 cacctcttgc gccgcggcgg cgcaggcgct ttccaaggtt tcggcggcct gcaggcggcg
     3001 gatggcgctg ttggtgcggc ggaagaacgc ctgcggctcg gcgacgtccc gctggggagg
     3061 ctccagctcc aggaagatga gctgatcatg gcgatgccag gagccgatga agcctgcgtc
     3121 ctttcgcatc gtgaagccga cagtgatcgg tgctccgacg gccgccccgg gctcggccaa
     3181 ggcgatcgtc agacggttgt gcgtctccga gtcgaagaca tcggccgcag agcggccgat
     3241 cagggcgccg atcgccagtc cggtgagttc gggaaggttg tcgctgccgg caacgatcgt
     3301 catgtcggcg gcgagggcga gcagcagtcc atgcggttgg atggcaccgg ggatatggat
     3361 cggctcatcg tcgcaggtcg gctgcacgtt ttccaccatc ttcaggaact cgtcgaaatc
     3421 gattctgccg ttcttgtcgg tgtcggagtc gccgaagatc tcgtccacgt cctcgtcggt
     3481 cagctgctcg ccggtcagtc tgatgatgtc gccaaactcc tctctgtcga tgaagccgtt
     3541 gccgtcgaag tcgaagattc tgaagcactc gctcagttcc tcttcgcttg gcggctgttg
     3601 aatagcaatt cccatgacga cagcttgctg tttcaggaag tcgatttccc aggtctgcca
     3661 ctcccgaggg ccggccagtt ggtgggccac cagcaggccc cacagcttct tgccttgcag
     3721 gatggggaca gccagaatgg cccgcacctg aaactcgatc agcaggtctc tgtagcactc
     3781 gaccagattg gctgtgtaga tgtcggcgat ggcctggtat ctgccgtgga cgtattcctc
     3841 gcctctggtt tccatgaagt atctgtcctg cacctgggtc ttcaggatgc taatccatct
     3901 gtcgtccacg gcttcgtgcg agacggtgcc ggagtaatct tcctcgaact tgaacacgca
     3961 cactctgtcc gcttgcagga acttgcgcac ctcggtcact gttgtattga gcattttatc
     4021 gagattggcc atggtaccag ctgcagatct gccatggtga tggtgatggt ggctccccat
     4081 ggttaattcc tcctgttagc ccaaaaaacg ggtatggaga aacagtagag agttgcgata
     4141 aaaagcgtca ggtaggatcc gctaatctta tggataaaaa tgctatggca tagcaaagtg
     4201 tgacgccgtg caaataatca atgtggactt ttctgccgtg attatagaca cttttgttac
     4261 gcgtttttgt catggctttg gtcccgcttt gttacagaat gcttttaata agcggggtta
     4321 ccggtttggt tagcgagaag agccagtaaa agacgcagtg acggcaatgt ctgatgcaat
     4381 atggacaatt ggtttcttct ctgaatggcg ggagtatgaa aagtatggct gaagcgcaaa
     4441 atgatcccct gctgccggga tactcgttta atgcccatct ggtggcgggt ttaacgccga
     4501 ttgaggccaa cggttatctc gattttttta tcgaccgacc gctgggaatg aaaggttata
     4561 ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa tcagggacga gaatttgttt
     4621 gccgaccggg tgatattttg ctgttcccgc caggagagat tcatcactac ggtcgtcatc
     4681 cggaggctcg cgaatggtat caccagtggg tttactttcg tccgcgcgcc tactggcatg
     4741 aatggcttaa ctggccgtca atatttgcca atacggggtt ctttcgcccg gatgaagcgc
     4801 accagccgca tttcagcgac ctgtttgggc aaatcattaa cgccgggcaa ggggaagggc
     4861 gctattcgga gctgctggcg ataaatctgc ttgagcaatt gttactgcgg cgcatggaag
     4921 cgattaacga gtcgctccat ccaccgatgg ataatcgggt acgcgaggct tgtcagtaca
     4981 tcagcgatca cctggcagac agcaattttg atatcgccag cgtcgcacag catgtttgct
     5041 tgtcgccgtc gcgtctgtca catcttttcc gccagcagtt agggattagc gtcttaagct
     5101 ggcgcgagga ccaacgtatc agccaggcga agctgctttt gagcaccacc cggatgccta
     5161 tcgccaccgt cggtcgcaat gttggttttg acgatcaact ctatttctcg cgggtattta
     5221 aaaaatgcac cggggccagc ccgagcgagt tccgtgccgg ttgtgaagaa aaagtgaatg
     5281 atgtagccgt caagttgtca taattggtaa cgaatcagac aattgacggc ttgacggagt
     5341 agcatagggt ttgcagaatc cctgcttcgt ccatttgaca ggcacattat gcatgccgct
     5401 tcgccttcgc gcgcgaattg atctgctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     5461 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     5521 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatga
//
