LOCUS       sequence-367240-        1733 bp    DNA     circular SYN 15-APR-2025
DEFINITION  2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use 
            in bi-cistronic and as the first 2A peptide in tri-cistronic 
            construct assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Mukherjee M, Wang ZQ
  TITLE     A Well-Characterized Polycistronic-Like Gene Expression System in 
            Yeast.
  JOURNAL   Biotechnol Bioeng. 2022 Sep 27. doi: 10.1002/bit.28247.
  PUBMED    36168285
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2022 Sep 27. doi: 10.1002/bit.28247."
COMMENT     Sequence Label: pYTK001_E2Ab
FEATURES             Location/Qualifiers
     source          1..1733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     336..355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(634..668)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(739..1398)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1313..1332
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      join(1580..1733,1..435)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
       61 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      121 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      181 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      241 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      301 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      361 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      421 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      481 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg
      541 ggtctcggtt ctggtggcgc taccaatttc tcactactaa aactggcggg tgacgtcgaa
      601 ttgaatcccg gccctaggag accgaccaga ccaataaaaa acgcccggcg gcaaccgagc
      661 gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa caggagtcca
      721 agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc
      781 attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag
      841 cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa
      901 gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc
      961 tgaaacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta
     1021 acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact
     1081 ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact
     1141 atcccatatc accagctcac cgtctttcat tgccatacga aattccggat gagcattcat
     1201 caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt
     1261 ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga
     1321 ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc
     1381 agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa
     1441 tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgcccgat
     1501 caatcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt cta
//
