LOCUS       sequence-367361-       11560 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Baculovirus protein expression of mouse TLR4 (residues 26–544) fused
            with hagfish variable lymphocyte receptor (VLR) (residues 126–200).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11560)
  AUTHORS   Wang Y, Su L, Morin MD, Jones BT, Whitby LR, Surakattula MM, Huang 
            H, Shi H, Choi JH, Wang KW, Moresco EM, Berger M, Zhan X, Zhang H, 
            Boger DL, Beutler B
  TITLE     TLR4/MD-2 activation by a synthetic agonist with no similarity to 
            LPS.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):E884-93. doi: 
            10.1073/pnas.1525639113. Epub 2016 Feb 1.
  PUBMED    26831104
REFERENCE   2  (bases 1 to 11560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1525639113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-02-16- 16"; volume: "113"; issue: "7"; pages: "E884-93"
COMMENT     Sequence Label: pAcGP67A-mTLR4ecd-VLR
FEATURES             Location/Qualifiers
     source          1..11560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_recomb     3170..3997
                     /label=baculovirus recombination region (lef2/ORF603)
                     /note="contains ORF603 and part of lef2"
     CDS             3170..3294
                     /codon_start=3
                     /product="baculovirus late expression factor 2"
                     /label=lef2
                     /translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
     CDS             complement(3332..3937)
                     /codon_start=1
                     /product="baculovirus ORF603 protein"
                     /label=ORF603
                     /translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
                     IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
                     KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
                     INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
     promoter        4001..4092
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     4017..4034
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     sig_peptide     4189..4248
                     /codon_start=1
                     /gene="Autographa californica gp64"
                     /product="signal sequence from the baculovirus envelope 
                     glycoprotein GP64"
                     /label=GP64 signal sequence
                     /translation="MVSAIVLYVLLAAAAHSAFA"
     misc_recomb     6568..7273
                     /label=baculovirus recombination region (ORF1629)
                     /note="contains part of ORF1629"
     3'UTR           6656..7028
                     /label=polyhedrin 3' UTR
                     /note="3' UTR from the Autographa californica 
                     nucleopolyhedrovirus polyhedrin gene (Suzuki et al., 2006)"
     CDS             complement(6685..7273)
                     /codon_start=2
                     /product="baculovirus capsid-associated protein"
                     /label=ORF1629
                     /note="required for viral replication"
                     /translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
                     PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
                     LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
                     ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
     CDS             complement(7707..7724)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8941..8957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8941..8957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8954..8976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8965..8981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8989..8995,8996..9013,9014..9019))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9034..9055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9172..9189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9343..9931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9423..9442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10102..10893,10894..10962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10725..10744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10963..11067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11135..11153
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11191..11213)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11313..11332
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11526..11548
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11540..11557
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11541..11557
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttggct cgtaaagcga gttgaaggat catatttagt tgcgtttatg agataagatt
       61 gaaagcacgt gtaaaatgtt tcccgcgcgt tggcacaact atttacaatg cggccaagtt
      121 ataaaagatt ctaatctgat atgttttaaa acacctttgc ggcccgagtt gtttgcgtac
      181 gtgactagcg aagaagatat gtggaccgca gaacagatag taaaacaaaa ccctagtatt
      241 ggagcaataa tcgatttaac caacacgtct aaatattatg atggtgtgca ttttttgcgg
      301 gcgggcctgt tatacaaaaa aattcaagta cctggccaga ctttgccgcc tgaaagcata
      361 gttcaagaat ttattgacac ggtaaaagaa tttacagaaa agtgtcccgg catgttggtg
      421 ggcgtgcact gcacacacgg tattaatcgc accggttaca tggtgtgcag atatttaatg
      481 cacaccctgg gtattgcgcc gcaggaagcc atagatagat tcgaaaaagc cagaggtcac
      541 aaaattgaaa gacaaaatta cgttcaagat ttattaattt aattaatatt atttgcattc
      601 tttaacaaat actttatcct attttcaaat tgttgcgctt cttccagcga accaaaacta
      661 tgcttcgctt gctccgttta gcttgtagcc gatcagtggc gttgttccaa tcgacggtag
      721 gattaggccg gatattctcc accacaatgt tggcaacgtt gatgttacgt ttatgctttt
      781 ggttttccac gtacgtcttt tggccggtaa tagccgtaaa cgtagtgccg tcgcgcgtca
      841 cgcacaacac cggatgtttg cgcttgtccg cggggtattg aaccgcgcga tccgacaaat
      901 ccaccacttt ggcaactaaa tcggtgacct gcgcgtcttt tttctgcatt atttcgtctt
      961 tcttttgcat ggtttcctgg aagccggtgt acatgcggtt tagatcagtc atgacgcgcg
     1021 tgacctgcaa atctttggcc tcgatctgct tgtccttgat ggcaacgatg cgttcaataa
     1081 actcttgttt tttaacaagt tcctcggttt tttgcgccac caccgcttgc agcgcgtttg
     1141 tgtgctcggt gaatgtcgca atcagcttag tcaccaactg tttgctctcc tcctcccgtt
     1201 gtttgatcgc gggatcgtac ttgccggtgc agagcacttg aggaattact tcttctaaaa
     1261 gccattcttg taattctatg gcgtaaggca atttggactt cataatcagc tgaatcacgc
     1321 cggatttagt aatgagcact gtatgcggct gcaaatacag cgggtcgccc cttttcacga
     1381 cgctgttaga ggtagggccc ccattttgga tggtctgctc aaataacgat ttgtatttat
     1441 tgtctacatg aacacgtata gctttatcac aaactgtata ttttaaactg ttagcgacgt
     1501 ccttggccac gaaccggacc tgttggtcgc gctctagcac gtaccgcagg ttgaacgtat
     1561 cttctccaaa tttaaattct ccaattttaa cgcgagccat tttgatacac gtgtgtcgat
     1621 tttgcaacaa ctattgtttt ttaacgcaaa ctaaacttat tgtggtaagc aataattaaa
     1681 tatgggggaa catgcgccgc tacaacactc gtcgttatga acgcagacgg cgccggtctc
     1741 ggcgcaagcg gctaaaacgt gttgcgcgtt caacgcggca aacatcgcaa aagccaatag
     1801 tacagttttg atttgcatat taacggcgat tttttaaatt atcttattta ataaatagtt
     1861 atgacgccta caactccccg cccgcgttga ctcgctgcac ctcgagcagt tcgttgacgc
     1921 cttcctccgt gtggccgaac acgtcgagcg ggtggtcgat gaccagcggc gtgccgcacg
     1981 cgacgcacaa gtatctgtac accgaatgat cgtcgggcga aggcacgtcg gcctccaagt
     2041 ggcaatattg gcaaattcga aaatatatac agttgggttg tttgcgcata tctatcgtgg
     2101 cgttgggcat gtacgtccga acgttgattt gcatgcaagc cgaaattaaa tcattgcgat
     2161 tagtgcgatt aaaacgttgt acatcctcgc ttttaatcat gccgtcgatt aaatcgcgca
     2221 atcgagtcaa gtgatcaaag tgtggaataa tgttttcttt gtattcccga gtcaagcgca
     2281 gcgcgtattt taacaaacta gccatcttgt aagttagttt catttaatgc aactttatcc
     2341 aataatatat tatgtatcgc acgtcaagaa ttaacaatgc gcccgttgtc gcatctcaac
     2401 acgactatga tagagatcaa ataaagcgcg aattaaatag cttgcgacgc aacgtgcacg
     2461 atctgtgcac gcgttccggc acgagctttg attgtaataa gtttttacga agcgatgaca
     2521 tgacccccgt agtgacaacg atcacgccca aaagaactgc cgactacaaa attaccgagt
     2581 atgtcggtga cgttaaaact attaagccat ccaatcgacc gttagtcgaa tcaggaccgc
     2641 tggtgcgaga agccgcgaag tatggcgaat gcatcgtata acgtgtggag tccgctcatt
     2701 agagcgtcat gtttagacaa gaaagctaca tatttaattg atcccgatga ttttattgat
     2761 aaattgaccc taactccata cacggtattc tacaatggcg gggttttggt caaaatttcc
     2821 ggactgcgat tgtacatgct gttaacggct ccgcccacta ttaatgaaat taaaaattcc
     2881 aattttaaaa aacgcagcaa gagaaacatt tgtatgaaag aatgcgtaga aggaaagaaa
     2941 aatgtcgtcg acatgctgaa caacaagatt aatatgcctc cgtgtataaa aaaaatattg
     3001 aacgatttga aagaaaacaa tgtaccgcgc ggcggtatgt acaggaagag gtttatacta
     3061 aactgttaca ttgcaaacgt ggtttcgtgt gccaagtgtg aaaaccgatg tttaatcaag
     3121 gctctgacgc atttctacaa ccacgactcc aagtgtgtgg gtgaagtcat gcatctttta
     3181 atcaaatccc aagatgtgta taaaccacca aactgccaaa aaatgaaaac tgtcgacaag
     3241 ctctgtccgt ttgctggcaa ctgcaagggt ctcaatccta tttgtaatta ttgaataata
     3301 aaacaattat aaatgtcaaa tttgtttttt attaacgata caaaccaaac gcaacaagaa
     3361 catttgtagt attatctata attgaaaacg cgtagttata atcgctgagg taatatttaa
     3421 aatcattttc aaatgattca cagttaattt gcgacaatat aattttattt tcacataaac
     3481 tagacgcctt gtcgtcttct tcttcgtatt ccttctcttt ttcatttttc tcctcataaa
     3541 aattaacata gttattatcg tatccatata tgtatctatc gtatagagta aattttttgt
     3601 tgtcataaat atatatgtct tttttaatgg ggtgtatagt accgctgcgc atagtttttc
     3661 tgtaatttac aacagtgcta ttttctggta gttcttcgga gtgtgttgct ttaattatta
     3721 aatttatata atcaatgaat ttgggatcgt cggttttgta caatatgttg ccggcatagt
     3781 acgcagcttc ttctagttca attacaccat tttttagcag caccggatta acataacttt
     3841 ccaaaatgtt gtacgaaccg ttaaacaaaa acagttcacc tcccttttct atactattgt
     3901 ctgcgagcag ttgtttgttg ttaaaaataa cagccattgt aatgagacgc acaaactaat
     3961 atcacaaact ggaaatgtct atcaatatat agttgctgat atcatggaga taattaaaat
     4021 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     4081 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atctatgcta
     4141 ctagtaaatc agtcacacca aggcttcaat aaggaacaca caagcaagat ggtaagcgct
     4201 attgttttat atgtgctttt ggcggcggcg gcgcattctg cctttgcggc ggatcccaat
     4261 ccctgcatag aggtagttcc taatattacc taccaatgca tggatcagaa actcagcaaa
     4321 gtccctgatg acattccttc ttcaaccaag aacatagatc tgagcttcaa ccccttgaag
     4381 atcttaaaaa gctatagctt ctccaatttt tcagaacttc agtggctgga tttatccagg
     4441 tgtgaaattg aaacaattga agacaaggca tggcatggct tacaccacct ctcaaacttg
     4501 atactgacag gaaaccctat ccagagtttt tccccaggaa gtttctctgg actaacaagt
     4561 ttagagaatc tggtggctgt ggagacaaaa ttggcctctc tagaaagctt ccctattgga
     4621 cagcttataa ccttaaagaa actcaatgtg gctcacaatt ttatacattc ctgtaagtta
     4681 cctgcatatt tttccaatct gacgaaccta gtacatgtgg atctttctta taactatatt
     4741 caaactatta ctgtcaacga cttacagttt ctacgtgaaa atccacaagt caatctctct
     4801 ttagacatgt ctttgaaccc aattgacttc attcaagacc aagcctttca gggaattaag
     4861 ctccatgaac tgactctaag aggtaatttt aatagctcaa atataatgaa aacttgcctt
     4921 caaaacctgg ctggtttaca cgtccatcgg ttgatcttgg gagaatttaa agatgaaagg
     4981 aatctggaaa tttttgaacc ctctatcatg gaaggactat gtgatgtgac cattgatgag
     5041 ttcaggttaa catatacaaa tgatttttca gatgatattg ttaagttcca ttgcttggcg
     5101 aatgtttctg caatgtctct ggcaggtgta tctataaaat atctagaaga tgttcctaaa
     5161 catttcaaat ggcaatcctt atcaatcatt agatgtcaac ttaagcagtt tccaactctg
     5221 gatctaccct ttcttaaaag tttgacttta actatgaaca aagggtctat cagttttaaa
     5281 aaagtggccc taccaagtct cagctatcta gatcttagta gaaatgcact gagctttagt
     5341 ggttgctgtt cttattctga tttgggaaca aacagcctga gacacttaga cctcagcttc
     5401 aatggtgcca tcattatgag tgccaatttc atgggtctag aagagctgca gcacctggat
     5461 tttcagcact ctactttaaa aagggtcaca gaattctcag cgttcttatc ccttgaaaag
     5521 ctactttacc ttgacatctc ttatactaac accaaaattg acttcgatgg tatatttctt
     5581 ggcttgacca gtctcaacac attaaaaatg gctggcaatt ctttcaaaga caacaccctt
     5641 tcaaatgtct ttgcaaacac aacaaacttg acattcctgg atctttctaa atgtcaattg
     5701 gaacaaatat cttggggggt atttgacacc ctccatagac ttcaattatt aaatatgagt
     5761 cacaacaatc tattgttttt ggattcatcc cattataacc agctgtattc cctcaaagaa
     5821 ctggcgctgg ataccaacca gctgaaaagc gtgccggatg gcatttttga tcgcctgacc
     5881 agcctgcaga aaatttggct gcataccaac ccgtgggatt gcagctgccc gcgcattgat
     5941 tatctgagcc gctggctgaa caaaaacagc cagaaagaac agggcagcgc gaaatgcagc
     6001 ggcagcggca aaccggtgcg cagcattatt tgcccgacct aaggatcccg ggtaccttct
     6061 agaattccgg agcggccgct gcagatctga tcctttcctg ggacccggca agaaccaaaa
     6121 actcactctc ttcaaggaaa tccgtaatgt taaacccgac acgatgaagc ttgtcgttgg
     6181 atggaaagga aaagagttct acagggaaac ttggacccgc ttcatggaag acagcttccc
     6241 cattgttaac gaccaagaag tgatggatgt tttccttgtt gtcaacatgc gtcccactag
     6301 acccaaccgt tgttacaaat tcctggccca acacgctctg cgttgcgacc ccgactatgt
     6361 acctcatgac gtgattagga tcgtcgagcc ttcatgggtg ggcagcaaca acgagtaccg
     6421 catcagcctg gctaagaagg gcggcggctg cccaataatg aaccttcact ctgagtacac
     6481 caactcgttc gaacagttca tcgatcgtgt catctgggag aacttctaca agcccatcgt
     6541 ttacatcggt accgactctg ctgaagagga ggaaattctc cttgaagttt ccctggtgtt
     6601 caaagtaaag gagtttgcac cagacgcacc tctgttcact ggtccggcgt attaaaacac
     6661 gatacattgt tattagtaca tttattaagc gctagattct gtgcgttgtt gatttacaga
     6721 caattgttgt acgtatttta ataattcatt aaatttataa tctttagggt ggtatgttag
     6781 agcgaaaatc aaatgatttt cagcgtcttt atatctgaat ttaaatatta aatcctcaat
     6841 agatttgtaa aataggtttc gattagtttc aaacaagggt tgtttttccg aaccgatggc
     6901 tggactatct aatggatttt cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa
     6961 tctagctttg tcgatattcg tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa
     7021 aatattatgc gcttttgtat ttctttcatc actgtcgtta gtgtacaatt gactcgacgt
     7081 aaacacgtta aataaagctt ggacatattt aacatcgggc gtgttagctt tattaggccg
     7141 attatcgtcg tcgtcccaac cctcgtcgtt agaagttgct tccgaagacg attttgccat
     7201 agccacacga cgcctattaa ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga
     7261 gctttttgga attatttctg attgcgggcg tttttgggcg ggtttcaatc taactgtgcc
     7321 cgattttaat tcagacaaca cgttagaaag cgatggtgca ggcggtggta acatttcaga
     7381 cggcaaatct actaatggcg gcggtggtgg agctgatgat aaatctacca tcggtggagg
     7441 cgcaggcggg gctggcggcg gaggcggagg cggaggtggt ggcggtgatg cagacggcgg
     7501 tttaggctca aatgtctctt taggcaacac agtcggcacc tcaactattg tactggtttc
     7561 gggcgccgtt tttggtttga ccggtctgag acgagtgcga tttttttcgt ttctaatagc
     7621 ttccaacaat tgttgtctgt cgtctaaagg tgcagcgggt tgaggttccg tcggcattgg
     7681 tggagcgggc ggcaattcag acatcgatgg tggtggtggt ggtggaggcg ctggaatgtt
     7741 aggcacggga gaaggtggtg gcggcggtgc cgccggtata atttgttctg gtttagtttg
     7801 ttcgcgcacg attgtgggca ccggcgcagg cgccgctggc tgcacaacgg aaggtcgtct
     7861 gcttcgaggc agcgcttggg gtggtggcaa ttcaatatta taattggaat acaaatcgta
     7921 aaaatctgct ataagcattg taatttcgct atcgtttacc gtgccgatat ttaacaaccg
     7981 ctcaatgtaa gcaattgtat tgtaaagaga ttgtctcaag ctcgcacgcc gataacaagc
     8041 cttttcattt ttactacagc attgtagtgg cgagacactt cgctgtcgtc gacgtacatg
     8101 tatgctttgt tgtcaaaaac gtcgttggca agctttaaaa tatttaaaag aacatctctg
     8161 ttcagcacca ctgtgttgtc gtaaatgttg tttttgataa tttgcgcttc cgcagtatcg
     8221 acacgttcaa aaaattgatg cgcatcaatt ttgttgttcc tattattgaa taaataagat
     8281 tgtacagatt catatctacg attcgtcatg gccaccacaa atgctacgct gcaaacgctg
     8341 gtacaatttt acgaaaactg caaaaacgtc aaaactcggt ataaaataat caacgggcgc
     8401 tttggcaaaa tatctatttt atcgcacaag cccactagca aattgtattt gcagaaaaca
     8461 atttcggcgc acaattttaa cgctgacgaa ataaaagttc accagttaat gagcgaccac
     8521 ccaaatttta taaaaatcta ttttaatcac ggttccatca acaaccaagt gatcgtgatg
     8581 gactacattg actgtcccga tttatttgaa acactacaaa ttaaaggcga gctttcgtac
     8641 caacttgtta gcaatattat tagacagctg tgtgaagcgc tcaacgattt gcacaagcac
     8701 aatttcatac acaacgacat aaaactcgaa aatgtcttat atttcgaagc acttgatcgc
     8761 gtgtatgttt gcgattacgg attgtgcaaa cacgaaaact cacttagcgt gcacgacggc
     8821 acgttggagt attttagtcc ggaaaaaatt cgacaccaca actatgcacg ttcgtttgac
     8881 tggtacgccg tcggcgtgtt aacatacaag ttgctaaccg gcggccaatt cgtaatcatg
     8941 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9001 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9061 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     9121 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     9181 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     9241 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     9301 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     9361 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     9421 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     9481 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     9541 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     9601 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     9661 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     9721 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     9781 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     9841 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     9901 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     9961 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10021 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10081 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10141 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10201 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10261 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    10321 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    10381 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    10441 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    10501 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    10561 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    10621 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    10681 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    10741 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    10801 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    10861 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    10921 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    10981 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11041 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
    11101 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11161 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11221 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11281 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11341 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
    11401 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
    11461 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
    11521 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
//
