LOCUS       sequence-367481-        1106 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of Mjdim1, a methyltransferase used in m6A-SAC-seq..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Hu L, Liu S, Peng Y, Ge R, Su R, Senevirathne C, Harada BT, Dai Q, 
            Wei J, Zhang L, Hao Z, Luo L, Wang H, Wang Y, Luo M, Chen M, Chen J,
            He C
  TITLE     m(6)A RNA modifications are measured at single-base resolution 
            across the mammalian transcriptome.
  JOURNAL   Nat Biotechnol. 2022 Mar 14. pii: 10.1038/s41587-022-01243-z. doi: 
            10.1038/s41587-022-01243-z.
  PUBMED    35288668
REFERENCE   2  (bases 1 to 1106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2022 Mar 14. pii: 10.1038/s41587-022-01243-z. doi: 
            10.1038/s41587-022-01243-z."
COMMENT     Sequence Label: pET28a-SUMO-MjDim1
FEATURES             Location/Qualifiers
     source          1..1106
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             36..58
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             77..94
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..118
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             128..145
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             158..451
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
ORIGIN
        1 ggggcattgg cggtacattc ccctctagaa ataattttgt ttaactttaa gaaggagata
       61 taccatgggc agcagccatc accatcatca ccacgactac aaggacgacg atgacaagag
      121 cagcggcctg gtgccgcgcg gcagccatat ggctagcatg tcggactcag aagtcaatca
      181 agaagctaag ccagaggtca agccagaagt caagcctgag actcacatca atttaaaggt
      241 gtccgatgga tcttcagaga tcttcttcaa gatcaaaaag accactcctt taagaaggct
      301 gatggaagcg ttcgctaaaa gacagggtaa ggaaatggac tccttaagat tcttgtacga
      361 cggtattaga atccaagctg atcagacccc tgaagatttg gacatggagg ataacgatat
      421 tattgaggct cacagagaac agattggtgg atccatgttc aagccgaaaa aaaagctggg
      481 tcagtgcttt ctgatcgaca aaaactttgt taataaggcc gttgaaagcg ccaatctgac
      541 caaagatgat gttgttctgg aaattggtct tggcaaaggt attctgaccg aagaactggc
      601 aaaaaacgcc aaaaaagtgt atgtgatcga gatcgataaa agcctggaac cgtatgccaa
      661 taaactgaaa gaactgtaca acaacatcga aatcatttgg ggtgatgccc tgaaagtgga
      721 tctgaataaa ctggatttca ataaggttgt tgccaacctg ccgtatcaga ttagcagccc
      781 gattaccttt aaactgatta aacgtggttt tgatctggcc gttctgatgt atcagtatga
      841 atttgcaaaa cgcatggttg caaaagaggg caccaaagat tacggtcgtc tgagcgttgc
      901 agttcagagc cgtgcagatg ttgaaattgt tgcgaaagtt ccgcctagcg cattttatcc
      961 gaaaccgaaa gtttatagcg ccatcgttta aatcaaaccg aacaaaggca ataccacatc
     1021 gagaacgaaa ctttttcgat gatttctgcg ggcatctttc acatcgtaat aaaacgttcg
     1081 taaaccctga ttgatacccg caaaac
//
