LOCUS       sequence-367533-        9963 bp    DNA     circular SYN 14-APR-2025
DEFINITION  SpCas9 gRNA correcting mdx4cv nonsense mutation with ABE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Xu L, Zhang C, Li H, Wang P, Gao Y, Mokadam NA, Ma J, Arnold WD, Han
            R
  TITLE     Efficient precise in vivo base editing in adult dystrophic mice.
  JOURNAL   Nat Commun. 2021 Jun 17;12(1):3719. doi: 10.1038/s41467-021-23996-y.
  PUBMED    34140489
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-06-17"; volume: "12(1):3719. doi"; pages: " 
            10.1038/s41467-021-23996-y"
COMMENT     Sequence Label: pLenti‐puro‐OgRNA_mdxE53
FEATURES             Location/Qualifiers
     source          1..9963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(86..105)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        277..656
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        658..856
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     811..831
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             874..1054
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1101..1226
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1719..1952
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2137..2181
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2479..2596
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2654..2902
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2654..2674
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2825..2844
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        3110..4288
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3341..4279
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4236..4256
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4295..4669
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    4694..5282
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4747..4767)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5165..5176)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5285..5301)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5286..5302)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5807..5987
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6013..6030)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6019..6243
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6289..6717
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6376..6395)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6586..6607
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6726..6746)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6731..7060
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6911..7046
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6973..6992
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7108..7155
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7174..7548
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7678..7799
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7715..7734)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7769..7788
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7848..7864)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7848..7864)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7861..7883)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7872..7888
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7896..7902,7903..7920,7921..7926))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7941..7962
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8079..8096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8250..8838)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8330..8349)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9009..9800,9801..9869))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9632..9651
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9870..9963,1..11))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
       61 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
      121 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
      181 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
      241 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
      301 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      361 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      421 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      481 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      541 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      601 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      661 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      721 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      781 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      841 aggtctatat aagcagcgcg ttttgcctgt actgggtctc tctggttaga ccagatctga
      901 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      961 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     1021 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     1081 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     1141 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     1201 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     1261 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     1321 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     1381 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1441 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1501 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1561 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1621 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1681 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1741 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1801 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1861 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1921 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1981 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     2041 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     2101 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     2161 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     2221 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     2281 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     2341 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     2401 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     2461 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     2521 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     2581 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttaattag
     2641 ctagcaggta cctgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     2701 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
     2761 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     2821 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     2881 cttgtggaaa ggacgaaaca ccggttatct cctgttctgc agcgtttcag agctatgctg
     2941 gaaacagcat agcaagttga aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     3001 agtcggtgct tttttactag tgcggatcct gcaaagatgg ataaagtttt aaacagagag
     3061 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc
     3121 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     3181 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     3241 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     3301 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     3361 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
     3421 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     3481 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     3541 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     3601 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     3661 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     3721 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     3781 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     3841 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     3901 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatgaagga cgcggcgctc
     3961 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     4021 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     4081 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     4141 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     4201 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     4261 tttttttctt ccatttcagg tgtcgtgatg tacaatggcc aagttgacca gtgccgttcc
     4321 ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt
     4381 ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt
     4441 catcagcgcg gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg
     4501 cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc
     4561 ctccgggccg gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg
     4621 cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag caggactgag aattcgatat
     4681 caagcttatc ggtaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     4741 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     4801 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     4861 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     4921 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     4981 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     5041 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     5101 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     5161 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     5221 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     5281 gcatcgatac cgtcgacctc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     5341 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     5401 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     5461 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
     5521 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
     5581 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     5641 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
     5701 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
     5761 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
     5821 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5881 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5941 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttaaa
     6001 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     6061 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     6121 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     6181 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     6241 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     6301 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     6361 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     6421 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     6481 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     6541 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     6601 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     6661 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     6721 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     6781 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     6841 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     6901 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6961 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     7021 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     7081 atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc
     7141 atagtataat acgacaaggt gaggaactaa accatggcca agttgaccag tgccgttccg
     7201 gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct ggaccgaccg gctcgggttc
     7261 tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc
     7321 atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc
     7381 ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc
     7441 tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc
     7501 gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca cgtgctacga
     7561 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     7621 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     7681 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     7741 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     7801 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     7861 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7921 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7981 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8041 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     8101 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     8161 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     8221 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     8281 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     8341 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     8401 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     8461 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     8521 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     8581 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     8641 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     8701 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     8761 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     8821 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     8881 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     8941 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     9001 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     9061 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     9121 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     9181 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     9241 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     9301 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     9361 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     9421 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     9481 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     9541 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     9601 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     9661 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     9721 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     9781 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     9841 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     9901 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     9961 ata
//
