LOCUS       sequence-367966-       12590 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses Ki67∆Nterm-mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Cuylen S, Blaukopf C, Politi AZ, Muller-Reichert T, Neumann B, Poser
            I, Ellenberg J, Hyman AA, Gerlich DW
  TITLE     Ki-67 acts as a biological surfactant to disperse mitotic 
            chromosomes.
  JOURNAL   Nature. 2016 Jun 29;535(7611):308-12. doi: 10.1038/nature18610.
  PUBMED    27362226
REFERENCE   2  (bases 1 to 12590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2016-06-29- 29"; volume: "535"; issue: "7611"; 
            pages: "308-12"
COMMENT     Sequence Label: Ki67∆Nterm-mNeonGreen-IRESpuro2
FEATURES             Location/Qualifiers
     source          1..12590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        307..510
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     461..481
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1142..1153
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1508..1519
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2234..2245
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             2966..2977
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             4418..4429
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             7348..8058
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      7603..7612
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          8127..8356
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     8339..8358
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(8428..9002,9003..9005,9006..9014)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8595..8612)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8822..8841
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9034..9633
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9034..9053)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9530..9550
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(9769..9786)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9775..9999
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10046..10065
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10125..10141)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10125..10141)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10138..10160)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10149..10165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10173..10179,10180..10197,10198..10203))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10218..10239
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10356..10373)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10527..11115)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10607..10626)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11286..12077,12078..12146))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11909..11928
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12147..12251)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12326..12345)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(12517..12590,1..306)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
       61 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      121 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      181 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      241 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      301 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      361 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      421 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      481 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagcgc
      541 taccggactc agatctcgag ctcaagcttc gaaatgacta aaatgccctg ccagtcatta
      601 caaccagaac caataaacac cccaacacac acaaaacaac agttgaaggc atccctgggg
      661 aaagtaggtg tgaaagaaga gctcctagca gtcggcaagt tcacacggac gtcaggggag
      721 accacgcaca cgcacagaga gccagcagga gatggcaaga gcatcagaac gtttaaggag
      781 tctccaaagc agatcctgga cccagcagcc cgtgtaactg gaatgaagaa gtggccaaga
      841 acgcctaagg aagaggccca gtcactagaa gacctggctg gcttcaaaga gctcttccag
      901 acaccaggtc cctctgagga atcaatgact gatgagaaaa ctaccaaaat agcctgcaaa
      961 tctccaccac cagaatcagt ggacactcca acaagcacaa agcaatggcc taagagaagt
     1021 ctcaggaaag cagatgtaga ggaagaattc ttagcactca ggaaactaac accatcagca
     1081 gggaaagcca tgcttacgcc caaaccagca ggaggtgatg agaaagacat taaagcattt
     1141 atgggaactc cagtgcagaa actggacctg gcaggaactt tacctggcag caaaagacag
     1201 ctacagactc ctaaggaaaa ggcccaggct ctagaagacc tggctggctt taaagagctc
     1261 ttccagactc ctggtcacac cgaggaatta gtggctgctg gtaaaaccac taaaataccc
     1321 tgcgactctc cacagtcaga cccagtggac accccaacaa gcacaaagca acgacccaag
     1381 agaagtatca ggaaagcaga tgtagaggga gaactcttag cgtgcaggaa tctaatgcca
     1441 tcagcaggca aagccatgca cacgcctaaa ccatcagtag gtgaagagaa agacatcatc
     1501 atatttgtgg gaactccagt gcagaaactg gacctgacag agaacttaac cggcagcaag
     1561 agacggccac aaactcctaa ggaagaggcc caggctctgg aagacctgac tggctttaaa
     1621 gagctcttcc agacccctgg tcatactgaa gaagcagtgg ctgctggcaa aactactaaa
     1681 atgccctgcg aatcttctcc accagaatca gcagacaccc caacaagcac aagaaggcag
     1741 cccaagacac ctttggagaa aagggacgta cagaaggagc tctcagccct gaagaagctc
     1801 acacagacat caggggaaac cacacacaca gataaagtac caggaggtga ggataaaagc
     1861 atcaacgcgt ttagggaaac tgcaaaacag aaactggacc cagcagcaag tgtaactggt
     1921 agcaagaggc acccaaaaac taaggaaaag gcccaacccc tagaagacct ggctggcttg
     1981 aaagagctct tccagacacc agtatgcact gacaagccca cgactcacga gaaaactacc
     2041 aaaatagcct gcagatcaca accagaccca gtggacacac caacaagctc caagccacag
     2101 tccaagagaa gtctcaggaa agtggacgta gaagaagaat tcttcgcact caggaaacga
     2161 acaccatcag caggcaaagc catgcacaca cccaaaccag cagtaagtgg tgagaaaaac
     2221 atctacgcat ttatgggaac tccagtgcag aaactggacc tgacagagaa cttaactggc
     2281 agcaagagac ggctacaaac tcctaaggaa aaggcccagg ctctagaaga cctggctggc
     2341 tttaaagagc tcttccagac acgaggtcac actgaggaat caatgactaa cgataaaact
     2401 gccaaagtag cctgcaaatc ttcacaacca gacccagaca aaaacccagc aagctccaag
     2461 cgacggctca agacatccct ggggaaagtg ggcgtgaaag aagagctcct agcagttggc
     2521 aagctcacac agacatcagg agagactaca cacacacaca cagagccaac aggagatggt
     2581 aagagcatga aagcatttat ggagtctcca aagcagatct tagactcagc agcaagtcta
     2641 actggcagca agaggcagct gagaactcct aagggaaagt ctgaagtccc tgaagacctg
     2701 gccggcttca tcgagctctt ccagacacca agtcacacta aggaatcaat gactaatgaa
     2761 aaaactacca aagtatccta cagagcttca cagccagacc tagtggacac cccaacaagc
     2821 tccaagccac agcccaagag aagtctcagg aaagcagaca ctgaagaaga atttttagca
     2881 tttaggaaac aaacgccatc agcaggcaaa gccatgcaca cacccaaacc agcagtaggt
     2941 gaagagaaag acatcaacac gtttttggga actccagtgc agaaactgga ccagccagga
     3001 aatttacctg gcagcaatag acggctacaa actcgtaagg aaaaggccca ggctctagaa
     3061 gaactgactg gcttcagaga gcttttccag acaccatgca ctgataaccc cacgactgat
     3121 gagaaaacta ccaaaaaaat actctgcaaa tctccgcaat cagacccagc ggacacccca
     3181 acaaacacaa agcaacggcc caagagaagc ctcaagaaag cagacgtaga ggaagaattt
     3241 ttagcattca ggaaactaac accatcagca ggcaaagcca tgcacacgcc taaagcagca
     3301 gtaggtgaag agaaagacat caacacattt gtggggactc cagtggagaa actggacctg
     3361 ctaggaaatt tacctggcag caagagacgg ccacaaactc ctaaagaaaa ggccaaggct
     3421 ctagaagatc tggctggctt caaagagctc ttccagacac caggtcacac tgaggaatca
     3481 atgaccgatg acaaaatcac agaagtatcc tgcaaatctc cacaaccaga cccagtcaaa
     3541 accccaacaa gctccaagca acgactcaag atatccttgg ggaaagtagg tgtgaaagaa
     3601 gaggtcctac cagtcggcaa gctcacacag acgtcaggga agaccacaca gacacacaga
     3661 gagacagcag gagatggaaa gagcatcaaa gcgtttaagg aatctgcaaa gcagatgctg
     3721 gacccagcaa actatggaac tgggatggag aggtggccaa gaacacctaa ggaagaggcc
     3781 caatcactag aagacctggc cggcttcaaa gagctcttcc agacaccaga ccacactgag
     3841 gaatcaacaa ctgatgacaa aactaccaaa atagcctgca aatctccacc accagaatca
     3901 atggacactc caacaagcac aaggaggcgg cccaaaacac ctttggggaa aagggatata
     3961 gtggaagagc tctcagccct gaagcagctc acacagacca cacacacaga caaagtacca
     4021 ggagatgagg ataaaggcat caacgtgttc agggaaactg caaaacagaa actggaccca
     4081 gcagcaagtg taactggtag caagaggcag ccaagaactc ctaagggaaa agcccaaccc
     4141 ctagaagact tggctggctt gaaagagctc ttccagacac caatatgcac tgacaagccc
     4201 acgactcatg agaaaactac caaaatagcc tgcagatctc cacaaccaga cccagtgggt
     4261 accccaacaa tcttcaagcc acagtccaag agaagtctca ggaaagcaga cgtagaggaa
     4321 gaatccttag cactcaggaa acgaacacca tcagtaggga aagctatgga cacacccaaa
     4381 ccagcaggag gtgatgagaa agacatgaaa gcatttatgg gaactccagt gcagaaattg
     4441 gacctgccag gaaatttacc tggcagcaaa agatggccac aaactcctaa ggaaaaggcc
     4501 caggctctag aagacctggc tggcttcaaa gagctcttcc agacaccagg cactgacaag
     4561 cccacgactg atgagaaaac taccaaaata gcctgcaaat ctccacaacc agacccagtg
     4621 gacaccccag caagcacaaa gcaacggccc aagagaaacc tcaggaaagc agacgtagag
     4681 gaagaatttt tagcactcag gaaacgaaca ccatcagcag gcaaagccat ggacacacca
     4741 aaaccagcag taagtgatga gaaaaatatc aacacatttg tggaaactcc agtgcagaaa
     4801 ctggacctgc taggaaattt acctggcagc aagagacagc cacagactcc taaggaaaag
     4861 gctgaggctc tagaggacct ggttggcttc aaagaactct tccagacacc aggtcacact
     4921 gaggaatcaa tgactgatga caaaatcaca gaagtatcct gtaaatctcc acagccagag
     4981 tcattcaaaa cctcaagaag ctccaagcaa aggctcaaga tacccctggt gaaagtggac
     5041 atgaaagaag agcccctagc agtcagcaag ctcacacgga catcagggga gactacgcaa
     5101 acacacacag agccaacagg agatagtaag agcatcaaag cgtttaagga gtctccaaag
     5161 cagatcctgg acccagcagc aagtgtaact ggtagcagga ggcagctgag aactcgtaag
     5221 gaaaaggccc gtgctctaga agacctggtt gacttcaaag agctcttctc agcaccaggt
     5281 cacactgaag agtcaatgac tattgacaaa aacacaaaaa ttccctgcaa atctccccca
     5341 ccagaactaa cagacactgc cacgagcaca aagagatgcc ccaagacacg tcccaggaaa
     5401 gaagtaaaag aggagctctc agcagttgag aggctcacgc aaacatcagg gcaaagcaca
     5461 cacacacaca aagaaccagc aagcggtgat gagggcatca aagtattgaa gcaacgtgca
     5521 aagaagaaac caaacccagt agaagaggaa cccagcagga gaaggccaag agcacctaag
     5581 gaaaaggccc aacccctgga agacctggcc ggcttcacag agctctctga aacatcaggt
     5641 cacactcagg aatcactgac tgctggcaaa gccactaaaa taccctgcga atctccccca
     5701 ctagaagtgg tagacaccac agcaagcaca aagaggcatc tcaggacacg tgtgcagaag
     5761 gtacaagtaa aagaagagcc ttcagcagtc aagttcacac aaacatcagg ggaaaccacg
     5821 gatgcagaca aagaaccagc aggtgaagat aaaggcatca aagcattgaa ggaatctgca
     5881 aaacagacac cggctccagc agcaagtgta actggcagca ggagacggcc aagagcaccc
     5941 agggaaagtg cccaagccat agaagaccta gctggcttca aagacccagc agcaggtcac
     6001 actgaagaat caatgactga tgacaaaacc actaaaatac cctgcaaatc atcaccagaa
     6061 ctagaagaca ccgcaacaag ctcaaagaga cggcccagga cacgtgccca gaaagtagaa
     6121 gtgaaggagg agctgttagc agttggcaag ctcacacaaa cctcagggga gaccacgcac
     6181 accgacaaag agccggtagg tgagggcaaa ggcacgaaag catttaagca acctgcaaag
     6241 cggaagctgg acgcagaaga tgtaattggc agcaggagac agccaagagc acctaaggaa
     6301 aaggcccaac ccctggaaga tctggccagc ttccaagagc tctctcaaac accaggccac
     6361 actgaggaac tggcaaatgg tgctgctgat agctttacaa gcgctccaaa gcaaacacct
     6421 gacagtggaa aacctctaaa aatatccaga agagttcttc gggcccctaa agtagaaccc
     6481 gtgggagacg tggtaagcac cagagaccct gtaaaatcac aaagcaaaag caacacttcc
     6541 ctgcccccac tgcccttcaa gaggggaggt ggcaaagatg gaagcgtcac gggaaccaag
     6601 aggttgcgct gcatgccagc accagaggaa attgtggagg agctgccagc cagcaagaag
     6661 cagagggttg ctcccagggc aagaggcaaa tcatccgaac ccgtggtcat catgaagaga
     6721 agtttgagga cttctgcaaa aagaattgaa cctgcggaag agctgaacag caacgacatg
     6781 aaaaccaaca aagaggaaca caaattacaa gactcggtcc ctgaaaataa gggaatatcc
     6841 ctgcgctcca gacgccaaaa taagactgag gcagaacagc aaataactga ggtctttgta
     6901 ttagcagaaa gaatagaaat aaacagaaat gaaaagaagc ccatgaagac ctccccagag
     6961 atggacattc agaatccaga tgatggagcc cggaaaccca tacctagaga caaagtcact
     7021 gagaacaaaa ggtgcttgag gtctgctaga cagaatgaga gctcccagcc taaggtggca
     7081 gaggagagcg gagggcagaa gagtgcgaag gttctcatgc agaatcagaa agggaaagga
     7141 gaagcaggaa attcagactc catgtgcctg agatcaagaa agacaaaaag ccagcctgca
     7201 gcaagcactt tggagagcaa atctgtgcag agagtaacgc ggagtgtcaa gaggtgtgca
     7261 gaaaatccaa agaaggctga ggacaatgtg tgtgtcaaga aaataagaac cagaagtcat
     7321 agggacagtg aagatatttg ggatcctatg gtgagcaagg gcgaggagga taacatggcc
     7381 tctctcccag cgacacatga gttacacatc tttggctcca tcaacggtgt ggactttgac
     7441 atggtgggtc agggcaccgg caatccaaat gatggttatg aggagttaaa cctgaagtcc
     7501 accaagggtg acctccagtt ctccccctgg attctggtcc ctcatatcgg gtatggcttc
     7561 catcagtacc tgccctaccc tgacgggatg tcgcctttcc aggccgccat ggtagatggc
     7621 tccggatacc aagtccatcg cacaatgcag tttgaagatg gtgcctccct tactgttaac
     7681 taccgctaca cctacgaggg aagccacatc aaaggagagg cccaggtgaa ggggactggt
     7741 ttccctgctg acggtcctgt gatgaccaac tcgctgaccg ctgcggactg gtgcaggtcg
     7801 aagaagactt accccaacga caaaaccatc atcagtacct ttaagtggag ttacaccact
     7861 ggaaatggca agcgctaccg gagcactgcg cggaccacct acacctttgc caagccaatg
     7921 gcggctaact atctgaagaa ccagccgatg tacgtgttcc gtaagacgga gctcaagcac
     7981 tccaagaccg agctcaactt caaggagtgg caaaaggcct ttaccgatgt gatgggcatg
     8041 gacgagctgt acaagtaagc ggccgcatag ataactgatc cagtgtgctg gaattaattc
     8101 gctgtctgcg agggccagct gttggggtga gtactccctc tcaaaagcgg gcatgacttc
     8161 tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt
     8221 gatgcctttg agggtggccg cgtccatctg gtcagaaaag acaatctttt tgttgtcaag
     8281 cttgaggtgt ggcaggcttg agatctggcc atacacttga gtgacaatga catccacttt
     8341 gcctttctct ccacaggtgt ccactcccag gtccaactgc aggtcgagca tgcatctagg
     8401 gcggccaatt ccgcccctct cccccccccc ctctccctcc ccccccccta acgttactgg
     8461 ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt
     8521 gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc
     8581 taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc
     8641 agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg
     8701 gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc
     8761 tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa
     8821 atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg
     8881 tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa
     8941 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     9001 agcttgccac aacccacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc
     9061 ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac
     9121 taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg
     9181 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     9241 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     9301 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     9361 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     9421 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     9481 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     9541 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     9601 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     9661 accgaaagga gcgcacgacc ccatggctcc gaccgaagcc acccggggcg gccccgccga
     9721 ccccgcaccc gcccccgagg cccaccgact ctagagctcg ctgatcagcc tcgactgtgc
     9781 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     9841 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     9901 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     9961 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
    10021 gctggggctc gagtgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
    10081 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
    10141 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    10201 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    10261 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    10321 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    10381 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    10441 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    10501 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    10561 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    10621 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    10681 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    10741 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    10801 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    10861 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    10921 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
    10981 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    11041 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    11101 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    11161 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    11221 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    11281 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    11341 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    11401 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    11461 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    11521 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    11581 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    11641 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    11701 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    11761 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    11821 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    11881 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    11941 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    12001 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    12061 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    12121 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    12181 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    12241 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
    12301 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    12361 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
    12421 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
    12481 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
    12541 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
//
