LOCUS       sequence-368015-         846 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Codon-optimized bacterial expression plasmid. Expresses Annexin A7 
            proline rich domain with strep..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Yu C, Nelson SL, Meisl G, Ghirlando R, Deshmukh L
  TITLE     Phase Separation and Fibrillization of Human Annexin A7 Are Mediated
            by Its Proline-Rich Domain.
  JOURNAL   Biochemistry. 2023 Oct 3. doi: 10.1021/acs.biochem.3c00349.
  PUBMED    37788367
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2023 Oct 3. doi: 10.1021/acs.biochem.3c00349."
COMMENT     Sequence Label: GB1-His-TEV-A7-Strep (2-180)
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             172..189
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             190..201
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             241..258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             259..279
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             814..837
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
ORIGIN
        1 catatgcagt ataaactggc gctgaacggc aaaaccctga aaggcgaaac caccaccgaa
       61 gcggtggatg cggcgaccgc ggaaaaagtg tttaaacagt atgcgaacga taacggcgtg
      121 gatggcgaat ggacctatga tgatgcgacc aaaaccttta ccgtgaccga actggtgccg
      181 cgcggcagca ttgaaggccg cattcgcgaa aacctgaaag atagcggcct gtttctggaa
      241 catcatcatc atcatcatga aaacctgtat tttcagagct atccgggcta tccgccgacc
      301 ggctatccac cgtttccggg ttatccgccg gcgggccaag aaagctcctt tccgccgagc
      361 ggtcagtacc cgtatccgag cgggttcccg ccgatgggtg gcggcgctta tccgcaagtg
      421 ccgagcagcg gatatcctgg cgcgggcgga tatccggcgc cgggcggcta tccggcgcct
      481 ggaggttatc cgggcgctcc gcaacctggt ggcgcgccgt cttacccagg tgtgccaccg
      541 ggccaagggt ttggcgtgcc tccgggcggc gcgggttttt cggggtaccc gcagccgcca
      601 agtcagtcat acggcggcgg accggctcaa gtgccgctac ctggtggctt tccgggcggt
      661 caaatgccga gtcagtatcc gggcggccaa ccgacgtatc cgagtcagat taacaccgat
      721 agctttagca gttatcctgt gtttagcccg gtgagcctgg attatagcag cgaaccggcg
      781 accgtgaccc aagtgaccca aggcaccatt cgctggagcc atccgcagtt tgaaaaataa
      841 ggatcc
//
