LOCUS       sequence-368100-       12661 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12661)
  AUTHORS   Daniel K, Icha J, Horenburg C, Muller D, Norden C, Mansfeld J
  TITLE     Conditional control of fluorescent protein degradation by an 
            auxin-dependent nanobody.
  JOURNAL   Nat Commun. 2018 Aug 17;9(1):3297. doi: 10.1038/s41467-018-05855-5.
  PUBMED    30120238
REFERENCE   2  (bases 1 to 12661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2018-08-17"; volume: "9(1):3297. doi"; pages: " 
            10.1038/s41467-018-05855-5"
COMMENT     Sequence Label: pCAGGs-NLS-TIR1_P2A_NES-TIR1
FEATURES             Location/Qualifiers
     source          1..12661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    593..727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        741..1120
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1122..1399
                     /label=chicken beta-actin promoter
     intron          1400..2414
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2338..2360
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      2475..2484
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2496..2516
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2523..4244
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             4248..4274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4284..4340
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4350..4379
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4389..4415
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             4419..6143
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="TYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFVG
                     NCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEEL
                     RMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEVE
                     DRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTLA
                     KILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYPL
                     CAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELRV
                     FPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRLC
                     ILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSIA
                     FAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKGC
                     QVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     misc_feature    join(6168..6742,6743..6745,6746..6754)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6335..6352)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6562..6581
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6755..7354
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFPATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFPETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6755..6774)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7251..7271
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7435..7452)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7441..7665
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7712..7728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7712..7728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7725..7747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7736..7752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7760..7766,7767..7784,7785..7790))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7805..7826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7885..8079
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7931..8065
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8085..8219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(8458..9045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(9216..10007,10008..10076))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    10669..10803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(11042..11630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(11801..12592,12593..12661))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 actcttcctt tttcaatgac cgctttggaa aaacaaagac tgtatttcct ggaaattaat
       61 gtttattcaa taaactgtgt attcagctat attcacatag tggtgaggct gaaatgaggc
      121 gggaagaggc ggttggggct taattatatc aatttgggtg gccccacagc gcctccaagg
      181 cgccagtcct gttttgacaa gttgcctctg gaagcctctc ttctttttct ccagagtaag
      241 cggaggccag gggcccccgg cctctgctta atactaaaaa aaacagctgt tgtcatagta
      301 atgattgggt ggaaacattc caggcctggg tggagaggct ttttgcttcc tcttgcaaaa
      361 ccacactgac attccaggcc tgggtggaga ggctttttgc ttcctcttgc aaaaccacac
      421 tgccctctgg agggcagttg cctagcaact aattaaaaga ggatgtcgca cggccagctg
      481 cggtcagtta gtcacttcct gcttaactga cttgacattt tctattttaa gagtcgggag
      541 gaaaattact gtgttggagg ccctccgcca tcttctgaag ctgaatcgaa ttaacttgtt
      601 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
      661 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
      721 ctggatctga tatcatcgtc gacattgatt attgactagt tattaatagt aatcaattac
      781 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
      841 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
      901 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
      961 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     1021 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     1081 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt
     1141 ctgcttcact ctccccatct ccccccccct ccccaccccc aattttgtat ttatttattt
     1201 tttaattatt ttgtgcagcg atgggggcgg gggggggggg gggcgcgcgc cgggcggggc
     1261 ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag ccaatcagag
     1321 cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa
     1381 gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc
     1441 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
     1501 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     1561 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag
     1621 cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct
     1681 gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg
     1741 cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa
     1801 aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg
     1861 gctgcaaccc ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg
     1921 gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcgggggtgg cggcaggtgg
     1981 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     2041 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     2101 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     2161 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     2221 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     2281 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     2341 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     2401 tctttttcct acagctcccg ggcaacgtgc tggttgttgt gctgtctcat cattttggca
     2461 aagaattacc cgccgccacc atgggccccg ccgcccccaa gaagaagaga aaggtgctgg
     2521 acacgtactt ccctgaagaa gttgtggaac atatcttttc ttttcttcct gcccaaagag
     2581 accggaatac agtctctttg gtatgtaagg tttggtatga gattgaaagg ttgtcaagga
     2641 gaggagtgtt tgtgggaaac tgctatgcgg taagggctgg aagagtcgct gccagattcc
     2701 ccaatgtgag ggcacttaca gttaagggta aaccacattt tgctgatttc aatttggttc
     2761 ctccggactg gggaggttat gctggaccat ggattgaggc tgctgccaga ggttgtcatg
     2821 ggttggagga gttgaggatg aagaggatgg ttgtgtctga tgagtccctt gaacttctgg
     2881 cccggtcctt ccccagattc agagctttgg tcttgatttc ttgtgagggt ttctctactg
     2941 acggactcgc tgcagtcgct tcccactgta agttgttgag ggagttggac ctccaagaaa
     3001 atgaggttga ggacagagga ccaagatggt tgtcatgttt ccctgactca tgtacttccc
     3061 ttgtttcctt gaatttcgcg tgtatcaaag gagaggtcaa tgccgggagt cttgagagat
     3121 tggtctcaag aagtcccaac ttgcgatcac tgaggctcaa cagatctgtg agtgtggata
     3181 ccttggcaaa gattctcttg agaaccccca atttggagga ccttggaact gggaatctca
     3241 cggatgactt ccaaactgag tcttacttta agttgactag tgctttggaa aagtgtaaga
     3301 tgttgagatc actgtccgga ttctgggatg cttcacctgt ttgtctttcc ttcatctacc
     3361 ctctttgtgc ccagctgact ggactcaact tgtcctatgc tccaacactg gatgcgagtg
     3421 acttgaccaa gatgatttca agatgcgtga agcttcaaag actttgggtc ctcgattgta
     3481 tctccgacaa aggacttcaa gtggtggcat catcttgtaa ggatctccaa gagttgagag
     3541 tgtttccaag tgacttctat gtggcaggtt acagtgctgt aactgaggaa gggttggttg
     3601 ctgtctccct tggatgtccc aagttgaatt cactcttgta cttttgtcac caaatgacta
     3661 acgctgccct tgtgacagtg gccaaaaatt gccctaactt cacacgcttc agactttgta
     3721 tccttgaacc tgggaaacct gacgtggtca ccagtcagcc cttggacgaa ggatttgggg
     3781 cgattgtgag agaatgtaag ggactccaaa gactctctat cagtggactc ctgactgaca
     3841 aggtgtttat gtacattggt aagtacgcta agcagctgga aatgctgtct attgcctttg
     3901 ccggtgattc tgacaagggg atgatgcatg ttatgaatgg atgtaagaac ttgagaaagc
     3961 ttgagataag agacagtcca ttcggagatg cagccctcct tggtaacttc gcaagatacg
     4021 aaactatgag gtctctttgg atgtccagct gtaatgttac actcaaggga tgtcaagtgc
     4081 tggcctcaaa aatgccaatg ctgaatgtgg aggtgatcaa cgagagagat ggatccaatg
     4141 aaatggaaga gaaccacgga gatcttccca aggtggaaaa gttgtatgtc tacagaacca
     4201 ctgctggggc aagggatgac gctccaaact tcgtgaaaat actgggctac ccatacgatg
     4261 ttcctgacta tgcgggaagc ggagctacta acttcagcct gctgaagcag gctggagacg
     4321 tggaggagaa ccctggacct ttaattaagg aacagaagtt aataagcgaa gaagacttac
     4381 cccttcagct tcccccgcta gaaagactta cgctagggac gtacttccct gaagaagttg
     4441 tggaacatat cttttctttt cttcctgccc aaagagaccg gaatacagtc tctttggtat
     4501 gtaaggtttg gtatgagatt gaaaggttgt caaggagagg agtgtttgtg ggaaactgct
     4561 atgcggtaag ggctggaaga gtcgctgcca gattccccaa tgtgagggca cttacagtta
     4621 agggtaaacc acattttgct gatttcaatt tggttcctcc ggactgggga ggttatgctg
     4681 gaccatggat tgaggctgct gccagaggtt gtcatgggtt ggaggagttg aggatgaaga
     4741 ggatggttgt gtctgatgag tcccttgaac ttctggcccg gtccttcccc agattcagag
     4801 ctttggtctt gatttcttgt gagggtttct ctactgacgg actcgctgca gtcgcttccc
     4861 actgtaagtt gttgagggag ttggacctcc aagaaaatga ggttgaggac agaggaccaa
     4921 gatggttgtc atgtttccct gactcatgta cttcccttgt ttccttgaat ttcgcgtgta
     4981 tcaaaggaga ggtcaatgcc gggagtcttg agagattggt ctcaagaagt cccaacttgc
     5041 gatcactgag gctcaacaga tctgtgagtg tggatacctt ggcaaagatt ctcttgagaa
     5101 cccccaattt ggaggacctt ggaactggga atctcacgga tgacttccaa actgagtctt
     5161 actttaagtt gactagtgct ttggaaaagt gtaagatgtt gagatcactg tccggattct
     5221 gggatgcttc acctgtttgt ctttccttca tctaccctct ttgtgcccag ctgactggac
     5281 tcaacttgtc ctatgctcca acactggatg cgagtgactt gaccaagatg atttcaagat
     5341 gcgtgaagct tcaaagactt tgggtcctcg attgtatctc cgacaaagga cttcaagtgg
     5401 tggcatcatc ttgtaaggat ctccaagagt tgagagtgtt tccaagtgac ttctatgtgg
     5461 caggttacag tgctgtaact gaggaagggt tggttgctgt ctcccttgga tgtcccaagt
     5521 tgaattcact cttgtacttt tgtcaccaaa tgactaacgc tgcccttgtg acagtggcca
     5581 aaaattgccc taacttcaca cgcttcagac tttgtatcct tgaacctggg aaacctgacg
     5641 tggtcaccag tcagcccttg gacgaaggat ttggggcgat tgtgagagaa tgtaagggac
     5701 tccaaagact ctctatcagt ggactcctga ctgacaaggt gtttatgtac attggtaagt
     5761 acgctaagca gctggaaatg ctgtctattg cctttgccgg tgattctgac aaggggatga
     5821 tgcatgttat gaatggatgt aagaacttga gaaagcttga gataagagac agtccattcg
     5881 gagatgcagc cctccttggt aacttcgcaa gatacgaaac tatgaggtct ctttggatgt
     5941 ccagctgtaa tgttacactc aagggatgtc aagtgctggc ctcaaaaatg ccaatgctga
     6001 atgtggaggt gatcaacgag agagatggat ccaatgaaat ggaagagaac cacggagatc
     6061 ttcccaaggt ggaaaagttg tatgtctaca gaaccactgc tggggcaagg gatgacgctc
     6121 caaacttcgt gaaaatactg tgatagttaa ttaagaattc cgcccccccc ctctccctcc
     6181 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     6241 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     6301 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     6361 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     6421 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     6481 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     6541 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     6601 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     6661 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     6721 ctttgaaaaa cacgatgata atatggccac aaccatgacc gagtacaagc ccacggtgcg
     6781 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     6841 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     6901 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     6961 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     7021 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     7081 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcccgg ccaccgtcgg
     7141 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     7201 ggcggccgag cgcgccgggg tgcccgcctt cccggagacc tccgcgcccc gcaacctccc
     7261 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     7321 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     7381 gaccgaaagg agcgcacgac cccatgcatc gatgatctag agctcgctga tcagcctcga
     7441 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7501 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7561 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7621 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     7681 gaacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7741 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7801 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7861 gaaacctgtc gtgccagcgg atccgcatct caattagtca gcaaccatag tcccgcccct
     7921 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7981 actaattttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     8041 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
     8101 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     8161 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     8221 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     8281 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     8341 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     8401 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     8461 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     8521 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     8581 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     8641 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     8701 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     8761 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     8821 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     8881 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8941 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     9001 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaggat ctcaagaaga tcctttgatc
     9061 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9121 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9181 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9241 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9301 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9361 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9421 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9481 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9541 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9601 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9661 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9721 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9781 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9841 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9901 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9961 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    10021 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10081 ttcctttttc aatgaccgct ttggaaaaac aaagactgta tttcctggaa attaatgttt
    10141 attcaataaa ctgtgtattc agctatattc acatagtggt gaggctgaaa tgaggcggga
    10201 agaggcggtt ggggcttaat tatatcaatt tgggtggccc cacagcgcct ccaaggcgcc
    10261 agtcctgttt tgacaagttg cctctggaag cctctcttct ttttctccag agtaagcgga
    10321 ggccaggggc ccccggcctc tgcttaatac taaaaaaaac agctgttgtc atagtaatga
    10381 ttgggtggaa acattccagg cctgggtgga gaggcttttt gcttcctctt gcaaaaccac
    10441 actgacattc caggcctggg tggagaggct ttttgcttcc tcttgcaaaa ccacactgcc
    10501 ctctggaggg cagttgccta gcaactaatt aaaagaggat gtcgcacggc cagctgcggt
    10561 cagttagtca cttcctgctt aactgacttg acattttcta ttttaagagt cgggaggaaa
    10621 attactgtgt tggaggccct ccgccatctt ctgaagctga atcgaattaa cttgtttatt
    10681 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    10741 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    10801 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
    10861 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
    10921 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
    10981 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
    11041 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
    11101 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
    11161 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
    11221 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
    11281 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
    11341 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
    11401 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
    11461 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
    11521 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
    11581 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
    11641 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
    11701 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
    11761 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
    11821 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
    11881 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    11941 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    12001 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    12061 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    12121 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    12181 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    12241 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    12301 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    12361 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    12421 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    12481 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    12541 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    12601 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    12661 t
//
