LOCUS       sequence-369043-        8754 bp    DNA     circular SYN 15-APR-2025
DEFINITION  AttR3/R4 destination vector for ascidian electroporation with 
            AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined 
            pFOG ectodermal regulatory sequence..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero 
            C, Mueller F, Lemaire P
  TITLE     A multicassette Gateway vector set for high throughput and 
            comparative analyses in ciona and vertebrate embryos.
  JOURNAL   PLoS One. 2007. 2(9):e916.
  PUBMED    17878951
REFERENCE   2  (bases 1 to 8754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2007. 2(9):e916."
COMMENT     Sequence Label: 
            pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2
FEATURES             Location/Qualifiers
     source          1..8754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..28
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     10..27
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     31..47
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     32..48
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    65..188
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(617..922)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(719..738)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1242..1925)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     1840..1859
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1926..2028)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    complement(2206..2329)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2553..2573)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2601..2617)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2601..2617)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    2647..2671
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2705..2725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2813..5860
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(2815..2837)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    complement(5885..5909)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6247..6381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6284..6303)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6338..6357
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6417..6436)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6418..6436)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6523..6540)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6694..7282)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6774..6793)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7453..8244,8245..8313))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8076..8095
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8314..8418)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8486..8504
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8542..8564)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8664..8683
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acacatacga tttaggtgac actatagaac tcgaggtcga cggtatcgat aagcttgatg
       61 gggacaagtt tgtataataa agttgaacga gaaacgtaaa atgatataaa tatcaatata
      121 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
      181 tcactatgaa tcaactactt agatggtatt agtgacctgt agtcgaccga cagccttcca
      241 aatgttcttc gggtgatgct gccaacttag tcgaccgaca gccttccaaa tgttcttctc
      301 aaacggaatc gtcgtatcca gcctactcgc tattgtcctc aatgccgtat taaatcataa
      361 aaagaaataa gaaaaagagg tgcgagcctc ttttttgtgt gacaaaataa aaacatctac
      421 ctattcatat acgctagtgt catagtcctg aaaatcatct gcatcaagaa caatttcaca
      481 actcttatac ttttctctta caagtcgttc ggcttcatct ggattttcag cctctatact
      541 tactaaacgt gataaagttt ctgtaatttc tactgtatcg acctgcagac tggctgtgta
      601 taagggagcc tgacatttat attccccaga acatcaggtt aatggcgttt ttgatgtcat
      661 tttcgcggtg gctgagatca gccacttctt ccccgataac ggagaccggc acactggcca
      721 tatcggtggt catcatgcgc cagctttcat ccccgatatg caccaccggg taaagttcac
      781 gggagacttt atctgacagc agacgtgcac tggccagggg gatcaccatc cgtcgcccgg
      841 gcgtgtcaat aatatcactc tgtacatcca caaacagacg ataacggctc tctcttttat
      901 aggtgtaaac cttaaactgc atttcaccag cccctgttct cgtcagcaaa agagccgttc
      961 atttcaataa accgggcgac ctcagccatc ccttcctgat tttccgcttt ccagcgttcg
     1021 gcacgcagac gacgggcttc attctgcatg gttgtgctta ccagaccgga gatattgaca
     1081 tcatatatgc cttgagcaac tgatagctgt cgctgtcaac tgtcactgta atacgctgct
     1141 tcatagcata cctctttttg acatacttcg ggtatacata tcagtatata ttcttatacc
     1201 gcaaaaatca gcgcgcaaat acgcatactg ttatctggct tttagtaagc cggatccacg
     1261 cggcgtttac gccccccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg
     1321 ccgacatgga agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc
     1381 ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata
     1441 ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac
     1501 atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct
     1561 tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa
     1621 aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc
     1681 agctcaccgt ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga
     1741 atgtgaataa aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc
     1801 gtaatatcca gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca
     1861 aaatgttctt tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc
     1921 tccattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     1981 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt
     2041 cgccaaaagt tggcccaggg cttcccggta tcaacaggga caccaggatt tatttattct
     2101 gcgaagtgat cttccgtcac aggtatttat tcggcgcaaa gtgcgtcggg tgatgctgcc
     2161 aacttagtcg actacaggtc actaatacca tctaagtagt tgattcatag tgactggata
     2221 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     2281 atatttatat cattttacgt ttctcgttca acttttctat acaaagtggc aaagcttcgt
     2341 gtattgtacc ggcccattgt caatcatgca aacttgatat tatattgaca agagaagaag
     2401 gcagtttaaa ttaaaactct aaagtagaga gacattaatc tcagctgaca aggcaggtgg
     2461 tcacagtaag ttcatttaaa tagttggcca acaatagcct ttccaagaaa gtatttttgt
     2521 tccaggtcta tacaaaaata acacacataa cccaactttt gtatacaaag tggtccccat
     2581 cgaattcctg cagcccgggg gatccactag ttctagagga tccatttaaa taggcctttg
     2641 tttatcacaa gtttgtacaa aaaagcaggc tgcaagggta ccgagctcag aaaaaatgac
     2701 tgctccaaag aagaagcgta aggtaccggt gggtgaagac cagaaacagc acctcgaact
     2761 gagccgcgat attgcccagc gtttcaacgc gctgtatggc gagatcgatc ccgtcgtttt
     2821 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     2881 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     2941 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga
     3001 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca
     3061 gatgcacggt tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc
     3121 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga
     3181 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca
     3241 tctgtggtgc aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt
     3301 tgacctgagc gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg
     3361 gagtgacggc agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga
     3421 cgtctcgttg ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt
     3481 taatgatgat ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg
     3541 tgactaccta cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac
     3601 cgcgcctttc ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact
     3661 acgtctgaac gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc
     3721 ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg
     3781 tttccgcgag gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat
     3841 tcgaggcgtt aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac
     3901 gatggtgcag gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca
     3961 ttatccgaac catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga
     4021 tgaagccaat attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg
     4081 ctggctaccg gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc
     4141 gagtgtgatc atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct
     4201 gtatcgctgg atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc
     4261 cgacaccacg gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc
     4321 cttcccggct gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg
     4381 cccgctgatc ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa
     4441 atactggcag gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt
     4501 ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga
     4561 ttttggcgat acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg
     4621 cacgccgcat ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt
     4681 atccgggcaa accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct
     4741 cctgcactgg atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga
     4801 tgtcgctcca caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc
     4861 cgggcaactc tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc
     4921 cgggcacatc agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc
     4981 cgccgcgtcc cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct
     5041 gggtaataag cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg
     5101 cgataaaaaa caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa
     5161 cgacattggc gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa
     5221 ggcggcgggc cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc
     5281 tgatgcggtg ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat
     5341 cagccggaaa acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga
     5401 agtggcgagc gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt
     5461 agcagagcgg gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac
     5521 tgccgcctgt tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt
     5581 cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg
     5641 gcgcggcgac ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag
     5701 ccatcgccat ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat
     5761 ggggattggt ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc
     5821 cggtcgctac cattaccagt tggtctggtg tcaaaaataa taataaccgg gcaggccatg
     5881 tctgacccag ctttcttgta caaagtggtg ataaacttag atatcggaat tccagctgag
     5941 cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc
     6001 atgtctgccc gtatttcgcg taaggaaatc cattatgtac tatttaaaaa acacaaactt
     6061 ttggatgttc ggtttattct ttttctttta cttttttatc atgggagcct acttcccgtt
     6121 tttcccgatt tggctacatg acatcaacca tatcagcaaa agtgatacgg gtattatttt
     6181 tgccgctatt tctctgttct cgctattatt ccaaccgctg tttggtctgc tttctgacaa
     6241 actcggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6301 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6361 gtatcttatc atgtctggat cgacaaagtc aaagcggcca tcagatctgc cggtctccct
     6421 atagtgagtc gtattaattt cgataagcca ggttaacctg cattaatgaa tcggccaacg
     6481 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     6541 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6601 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     6661 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     6721 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     6781 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     6841 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6901 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6961 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7021 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7081 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7141 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7201 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7261 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7321 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7381 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7441 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7501 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7561 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7621 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7681 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     7741 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     7801 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     7861 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7921 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7981 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8041 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8101 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8161 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8221 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8281 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8341 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8401 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
     8461 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
     8521 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     8581 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     8641 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
     8701 ggacatattg tcgttagaac gcggctacaa ttaatacata accttatgta tcat
//
