LOCUS       sequence-369255-        8393 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Glutamate sensor for localization to membrane contatct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Shindo Y, Ashida K, Masamoto K, Takuwa H, Takahashi M, Higuchi M, 
            Ide R, Hotta K, Oka K
  TITLE     Genetically encoded sensors for analysing neurotransmission among 
            synaptically-connected neurons
  JOURNAL   bioRxiv 2022
REFERENCE   2  (bases 1 to 8393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022"
COMMENT     Sequence Label: pcDNA3.1-iRFP-P2A-Post-SPHERE-SF-iGluSnFR
FEATURES             Location/Qualifiers
     source          1..8393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1041..1060)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1232..1611
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1612..1815
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1766..1786
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1860..1879
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1860..1878
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1914..1923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1920..2852
                     /codon_start=1
                     /product="near-infrared fluorescent protein with an 
                     emission peak at 670 nm, engineered from a bacterial 
                     phytochrome (Shcherbakova and Verkhusha, 2013)"
                     /label=iRFP670
                     /note="derived from Rhodopseudomonas palustris RpBphP6"
                     /translation="MARKVDLTSCDREPIHIPGSIQPCGCLLACDAQAVRITRITENAG
                     AFFGRETPRVGELLADYFGETEAHALRNALAQSSDPKRPALIFGWRDGLTGRTFDISLH
                     RHDGTSIIEFEPAAAEQADNPLRLTRQIIARTKELKSLEEMAARVPRYLQAMLGYHRVM
                     LYRFADDGSGMVIGEAKRSDLESFLGQHFPASLVPQQARLLYLKNAIRVVSDSRGISSR
                     IVPEHDASGAALDLSFAHLRSISPCHLEFLRNMGVSASMSLSIIIDGTLWGLIICHHYE
                     PRAVPMAQRVAAEMFADFLSLHFTAAHHQR"
     CDS             2859..2900
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2910..2966
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     4214..4235
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(4572..4583)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4981..4998)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4987..5211
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5257..5685
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5344..5363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5554..5575
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5694..5714)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5699..6028
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5879..6014
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5941..5960
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6095..6889
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6149..6168)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6759..6778
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7063..7184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7100..7119)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7154..7173
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7233..7249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7233..7249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7246..7268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7257..7273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7281..7287,7288..7305,7306..7311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7326..7347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7464..7481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7635..8223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7715..7734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     1021 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     1081 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     1141 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     1201 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     1261 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     1321 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     1381 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     1441 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     1501 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     1561 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     1621 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     1681 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     1741 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     1801 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
     1861 aatacgactc actataggga gacccaagct ggctagcgtt taaacttaag cttgccacca
     1921 tggcgcgtaa ggtcgatctc acctcctgcg atcgcgagcc gatccacatc cccggcagca
     1981 ttcagccgtg cggctgcctg ctagcctgcg acgcgcaggc ggtgcggatc acgcgcatta
     2041 cggaaaatgc cggcgcgttc tttggacgcg aaactccgcg ggtcggtgag ctactcgccg
     2101 attacttcgg cgagaccgaa gcccatgcgc tgcgcaacgc actggcgcag tcctccgatc
     2161 caaagcgacc ggcgctgatc ttcggttggc gcgacggcct gaccggccgc accttcgaca
     2221 tctcactgca tcgccatgac ggtacatcga tcatcgagtt cgagcctgcg gcggccgaac
     2281 aggccgacaa tccgctgcgg ctgacgcggc agatcatcgc gcgcaccaaa gaactgaagt
     2341 cgctcgaaga gatggccgca cgggtgccgc gctatctgca ggcgatgctc ggctatcacc
     2401 gcgtgatgtt gtaccgcttc gcggacgacg gctccgggat ggtgatcggc gaggcgaagc
     2461 gcagcgacct cgagagcttt ctcggtcagc actttccggc gtcgctggtc ccgcagcagg
     2521 cgcggctact gtacttgaag aacgcgatcc gcgtggtctc ggattcgcgc ggcatcagca
     2581 gccggatcgt gcccgagcac gacgcctccg gcgccgcgct cgatctgtcg ttcgcgcacc
     2641 tgcgcagcat ctcgccctgc catctcgaat ttctgcggaa catgggcgtc agcgcctcga
     2701 tgtcgctgtc gatcatcatt gacggcacgc tatggggatt gatcatctgt catcattacg
     2761 agccgcgtgc cgtgccgatg gcgcagcgcg tcgcggccga aatgttcgcc gacttcttat
     2821 cgctgcactt caccgccgcc caccaccaac gcggatccgg caagcctatc cctaatcctc
     2881 tgctgggact ggactctaca ggaagcggag ctactaactt cagcctgctg aagcaggctg
     2941 gagacgtgga ggagaaccct ggacctgaga ccgacaccct cctgctatgg gtgctgctgc
     3001 tctgggtgcc cggcagcacc ggcgacgcac cggtcggagg caacgaccct aacctgttcg
     3061 tcgccctgta cgacttcgtc gcctctggcg acaacaccct gagcatcacc aagggcgaga
     3121 agctgagagt cctgggctac aaccacaacg gcgagtggtg cgaggcccag accaagaacg
     3181 gccagggctg ggtccctagc aactacatca cacctgtcaa cagcaccggt ggaggcagcg
     3241 gaggaggctc cggagccgca ggcagcacgc tggacaaaat cgccaaaaac ggtgtgattg
     3301 tcgtcggtca ccgtgaatct tcagtgcctt tctcttatta cgacaatcag caaaaagtgg
     3361 tgggttactc gcaggattac tccaacgcca ttgttgaagc agtgaaaaag aaactcaaca
     3421 aaccggactt gcaggtaaaa ctgattccga ttacctcaca aaaccgtatt ccactgctgc
     3481 aaaacggcac tttcgatttt gaatgtggtt ctaccaccaa caacgtcgaa cgccaaaaac
     3541 aggcggcttt ctctgacact attttcgtgg tcggtacgcg cctgttgacc aaaaagggtg
     3601 gcgatatcaa agattttgcc aacctgaaag acaaagccgt agtcgtcact tccggcacta
     3661 cctctgaagt tttgctcaac aaactgaatg aagagcaaaa aatgaatatg cgcatcatca
     3721 gcgccaaaga tcacggtgac tctttccgca ccctggaaag cggtcgtgcc gttgccttta
     3781 tgatggatga cgtgctgctg gccggtgaac gtgcgaaagc gaagaaacca gacaactggg
     3841 aaatcgtcgg caagccgcag tctcaggagg cctacggttg tatgttgcgt aaagatgatc
     3901 cgcagttcaa aaagctgatg gatgacacca tcgctcaggt gcagacctcc ggtgaagcgg
     3961 aaaaatggtt tgataagtgg ttcaaaaatc caattctggt gagccacaac gtctatatca
     4021 ccgccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacgtggagg
     4081 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4141 tgctgctgcc cgacaaccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg
     4201 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     4261 tggacgagct gtacaaggga ggaggctccg gaggcggaag cggtaccctc gagctggtgc
     4321 cccacttgca taacctcaat gacatcagcc aatacacaag tacaaccacc aaggtcccaa
     4381 gcactgacat cacccttcga ccaactcgga aaaactcaac ccctgtgacc agcgcatttc
     4441 caaccgctaa gcaagacgac cccaaacaac agcctagtcc atttagcgta gaccaaaggg
     4501 actacagcac agagctgtcc gtgaccatag ctgtaggagc ttccctgctt ttcctgaaca
     4561 ttctggcttt cgcggccctc tattacaaga aggacaaaag gcgccacgac gtacatagga
     4621 gatgtagtcc gcaacgaact acaaccaatg atcttacgca cgcacctgag gaggagatca
     4681 tgagtcttca gatgaaacac acagacctgg accacgagtg tgaatccatc catccccatg
     4741 aggtggtgct gaggacagca tgccctccgg actataccct ggcaatgcgc cgaagcccag
     4801 acgacattcc cctgatgaca cccaatacca taaccatgat tcccaacact attccaggaa
     4861 tccagcctct ccacactttc aacacattca ctggtggaca gaataatact ctccctcatc
     4921 cgcatccaca cccccactcc cacagctaat ctagagggcc cgtttaaacc cgctgatcag
     4981 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     5041 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     5101 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     5161 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     5221 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     5281 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5341 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5401 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5461 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5521 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5581 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5641 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     5701 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     5761 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     5821 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     5881 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     5941 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     6001 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     6061 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
     6121 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
     6181 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
     6241 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
     6301 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
     6361 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
     6421 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
     6481 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
     6541 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
     6601 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
     6661 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
     6721 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
     6781 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
     6841 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
     6901 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
     6961 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
     7021 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
     7081 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     7141 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
     7201 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     7261 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     7321 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7381 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7441 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     7501 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     7561 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     7621 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     7681 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7741 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7801 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     7861 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7921 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7981 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8041 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     8101 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8161 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8221 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8281 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8341 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cag
//
