LOCUS       sequence-36957-e         934 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich 
            A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R, 
            Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K, 
            Cookson MR
  TITLE     Kinase activity is required for the toxic effects of mutant 
            LRRK2/dardarin.
  JOURNAL   Neurobiol Dis. 2006 Aug . 23(2):329-41.
  PUBMED    16750377
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis. 2006 Aug . 23(2):329-41."
COMMENT     Sequence Label: pDEST53-LRRK2-WT
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(89..91,92..94,95..805)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(122..150)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     745..765
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    827..851
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctggc tagacaccat ggccagcaaa ggagaagaac ttttcactgg
      121 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      181 tggagagggt gaaggtgatg ctacatacgg aaagcttacc cttaaattta tttgcactac
      241 tggaaaacta cctgttccat ggccaacact tgtcactact ttctcttatg gtgttcaatg
      301 cttttcccgt tatccggatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga
      361 aggttatgta caggaacgca ctatatcttt caaagatgac gggaactaca agacgcgtgc
      421 tgaagtcaag tttgaaggtg atacccttgt taatcgtatc gagttaaaag gtattgattt
      481 taaagaagat ggaaacattc tcggacacaa actcgagtac aactataact cacacaatgt
      541 atacatcacg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa
      601 cattgaagat ggatccgttc aactagcaga ccattatcaa caaaatactc caattggcga
      661 tggccctgtc cttttaccag acaaccatta cctgtcgaca caatctgccc tttcgaaaga
      721 tcccaacgaa aagcgtgacc acatggtcct tcttgagttt gtaactgctg ctgggattac
      781 acatggcatg gatgagctct acaaaagcgg ttccggaccg gatcaaacaa gtttgtacaa
      841 aaaagcaggc tccgaattcg cccttatggc tagtggcagc tgtcaggggt gcgaagagga
      901 cgaggaaact ctgaagaagt tgatagtcag gctg
//
