LOCUS       sequence-369662-        5351 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Large terminase from phage PhiV1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5351)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 5351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: Terminase_PhiV1
FEATURES             Location/Qualifiers
     source          1..5351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..155
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(81..100)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     241..263
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(419..1297)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1324..1608
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1531..1550
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      3423..3450
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3469..3560
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3561..3629,3630..4421)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3779..3798)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4592..5180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5081..5100
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5334..5351
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
       61 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
      121 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
      181 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
      241 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagcaga
      301 tcaattcgcg cgcgaaggcg aagcggcatg cataatgtgc ctgtcaaatg gacgaagcag
      361 ggattctgca aaccctatgc tactccgtca agccgtcaat tgtctgattc gttaccaatt
      421 atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg aactcgctcg
      481 ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg tcaaaaccaa
      541 cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc ttcgcctggc
      601 tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg cggaaaagat
      661 gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata tcaaaattgc
      721 tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta tccatcggtg
      781 gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca agcagattta
      841 tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt tgcccaaaca
      901 ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta ttggcaaata
      961 ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa acccactggt
     1021 gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct ggcgggaaca
     1081 gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc accccctgac
     1141 cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg ataaaaaaat
     1201 cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca ttaaacgagt
     1261 atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc ccgccattca
     1321 gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc ttttactggc
     1381 tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa caaagcggga
     1441 ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga aaagtccaca
     1501 ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc ataagattag
     1561 cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac ccgttttttg
     1621 ggctaacagg aggaattaac catgagccag agccaagaag cgaagaacgc gctgatcatt
     1681 gcgcagctga agggtgactt tgtggcgttc ctgtttgttc tgtggaaggc gctgaacctg
     1741 ccgaagccga ccaaatgcca gattgacatg gcgcgtaccc tggcgaacgg cgatcacaag
     1801 aaattcatcc tgcaagcgtt tcgtggtatt ggcaaaagct tcatcacctg cgcgtttgtg
     1861 gtttgggtgc tgtggcgtga tccgcaactg aaggtgctga tcgttagcgc gagcaaagaa
     1921 cgtgcggacg cgaacagcat tttcatcaag aacatcattg atctgctgcc gtttctgagc
     1981 gagctgaaac cgcgtccggg tcagcgtgac agcgtgatta gctttgatgt gggcctggcg
     2041 aagccggacc acagcccgag cgtgaaaagc gttggtatca ccggccaact gaccggtagc
     2101 cgtgcggata tcattatcgc ggacgatgtt gaggtgccgg gtaacagcag caccagcagc
     2161 gcgcgtgaaa agctgtggac cctggtgacc gagtttgcgg cgctgctgaa accgctgccg
     2221 accagccgtg ttatttacct gggtaccccg cagaccgaaa tgaccctgta caaggaactg
     2281 gaggacaaca aaggctatag caccgtgatc tggccggcgc aatacccgcg taacgatgcg
     2341 gaggcgctgt actatggtga tcgtctggcg ccgatgctga aggcggagta cgatgaaggt
     2401 tttgagctgc tgcgtggtca gccgaccgac ccggttcgtt ttgatatgga cgatctgcgt
     2461 gaacgtgagc tggaatacgg caaggcgggc tataccctgc aatttatgct gaacccgaac
     2521 ctgagcgacg cggaaaaata tccgctgcgt ctgcgtgacg cgattgtttg cgcggttgat
     2581 ccggaacgtg cgccgctgag ctaccagtgg ctgccgaacc gtcaaaaccg taacgaggaa
     2641 ctgccgaacg tgggtctgaa aggcgacgat atccacgcgt tccacacctg cagcagccgt
     2701 accgcggaat atcagagcaa gattctggtt atcgacccga gcggtcgtgg caaagatgaa
     2761 accggttacg cggtgctgta tagcctgaac ggctacattt atctgatgga agttggtggc
     2821 tttcgtggtg gctacgacga tgcgaccctg gagaagctgg cgaagaaagc gaagcagtgg
     2881 aaagttcaaa ccgtggttca cgaaagcaac ttcggtgacg gcatgttcgg taaaattttt
     2941 agcccgatcc tgctgaagca ccacaaatgc gcgctggagg aaatccgtgc gaagggtatg
     3001 aaagaaatgc gtatttgcga caccattgag ccgctgatgg gtgcgcacaa gctggtgatt
     3061 cgtgacgaag ttatccgtga ggattatcag accgcgcgtg acctggatgg taaacacgat
     3121 gtgcgttaca gcgcgttcta tcaaatgacc cgtatgaccc gtgagcgtgg cgcggttgcg
     3181 catgacgatc gtattgacgc gatcgcgctg ggtatcgaat acctgcgtga gggcatgctg
     3241 gttgacagcc gtgttggcga ggaagagatg accctggaat tcctggagca ccacatggaa
     3301 aagcagacca ttggtggcga ccaaatccac agctttgacg tgggtggcgt tgatatttac
     3361 tatgaggacg aagatggtgg cagcagcttc atcgagtggt aatgccaggc atcaaattaa
     3421 gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttt tgtttatttt
     3481 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3541 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3601 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     3661 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     3721 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     3781 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     3841 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     3901 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     3961 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4021 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4081 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4141 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4201 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4261 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4321 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4381 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4441 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4501 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4561 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4621 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     4681 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     4741 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     4801 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     4861 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     4921 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4981 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5041 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5101 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5161 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5221 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5281 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5341 cagtgagcga g
//
