LOCUS       sequence-369721-        2300 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Spacer cassette with ConL8 and ConRE connectors to be used in the 
            Dueber YTK and Ellis MYT systems.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Shaw WM, Khalil AS, Ellis T
  TITLE     A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   ACS Synth Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423.
  PUBMED    37930278
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 6. doi: 10.1021/acssynbio.3c00423."
COMMENT     Sequence Label: pMYT072
FEATURES             Location/Qualifiers
     source          1..2300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(811..1399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(891..910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1502..1606
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1607..1675,1676..2300,1..167)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1825..1844)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatctggagc cggtgagcgt gggtcccgcg gtatcattgc agcactgggg ccagatggta
       61 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
      121 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
      181 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
      241 tgaagatcct ttttgataat cgcggccgct ggactgttgt gatatgccat atatgcagtg
      301 gtgttcgccg gcgctggttc cggctgtctt gcttagttgt gagtcgccag gcagtcatcg
      361 gtatgatctg tacatgattc gtcagtgtga gcaccactga cgagcagatt tcgctgacat
      421 gaatatgctg gtccctgcag caacactagt gtgtcgtctc atgctgactg gtttcgtcta
      481 ccgccatgct accaccaaat acggattgga gtgagacgtc cttctagaga aggaattcgt
      541 ccgtcattgc atctaggtgc ctaccaacct cagtaatacg aaacggattg acctgctcct
      601 ctcgccgaag aagtccctgg aatagtaaaa actgcccaac gcacgattgc cttatctgta
      661 gacacggtcc tgtgactgaa ctctcccgac gaaatagaat acggcggccg cacggttatc
      721 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
      781 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
      841 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
      901 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
      961 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1021 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     1081 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     1141 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     1201 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     1261 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     1321 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     1381 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     1441 gaacgaaaac tcacgttaag ggattttggt catgacaggt ggcacttttc ggggaaatgt
     1501 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     1561 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1621 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1681 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1741 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1801 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     1861 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1921 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1981 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2041 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2101 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     2161 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     2221 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     2281 tggctggttt attgctgata
//
