LOCUS       sequence-369865-        9803 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Codon optimized SeAvs3 with C-terminal His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9803)
  AUTHORS   Gao LA, Wilkinson ME, Strecker J, Makarova KS, Macrae RK, Koonin EV,
            Zhang F
  TITLE     Prokaryotic innate immunity through pattern recognition of conserved
            viral proteins.
  JOURNAL   Science. 2022 Aug 12;377(6607):eabm4096. doi: 
            10.1126/science.abm4096. Epub 2022 Aug 12.
  PUBMED    35951700
REFERENCE   2  (bases 1 to 9803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2022-08-12"; volume: "377(6607):eabm4096. doi"; pages: " 
            10.1126/science.abm4096. Epub 2022 Aug 12"
COMMENT     Sequence Label: pCDF-SeAvs3
FEATURES             Location/Qualifiers
     source          1..9803
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        759..850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             851..1642
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      complement(1813..1860)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     1856..1874
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(1906..1923)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    8252..8276
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(8276..8295)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8277..8295)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        8341..8418
                     /gene="lacI"
                     /label=lacI promoter
     CDS             8419..9501
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8438..8457)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 ttaagcagtt ccccaactga cttaaccttc gaaaaaacca cctggggagg tggtttgatc
       61 gaaggttaag tcagttgggg aactgcttaa ccgtggtaac tggctttcgc agagcacagc
      121 aaccaaatct gtccttccag tgtagccgga ctttggcgca cacttcaaga gcaaccgcgt
      181 gtttagctaa acaaatcctc tgcgaactcc cagttaccaa tggctgctgc cagtggcgtt
      241 ttaccgtgct tttccgggtt ggactcaagt gaacagttac cggataaggc gcagcagtcg
      301 ggctgaacgg ggagttcttg cttacagccc agcttggagc gaacgaccta caccgagccg
      361 agataccagt gtgtgagcta tgagaaagcg ccacacttcc cgtaagggag aaaggcggaa
      421 caggtatccg gtaaacggca gggtcggaac aggagagcgc aagagggagc gacccgccgg
      481 aaacggtggg gatctttaag tcctgtcggg tttcgcccgt actgtcagat tcatggttga
      541 gcctcacggc tcccacagat gcaccggaaa agcgtctgtt tatgtgaact ctggcaggag
      601 ggcggagcct atggaaaaac gccaccggcg cggccctgct gttttgcctc acatgttagt
      661 cccctgctta tccacggaat ctgtgggtaa ctttgtatgt gtccgcagcg cccgccgcag
      721 tctcacgccc ggagcgtagc gaccgagtga gctagctatt tgtttatttt tctaaataca
      781 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      841 aaggaagagt atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt
      901 tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc
      961 agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag
     1021 gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc
     1081 tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat
     1141 tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat
     1201 tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa
     1261 agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt
     1321 tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc
     1381 cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc
     1441 agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc
     1501 ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga
     1561 tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat
     1621 caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgagggg ccgcaagatc
     1681 cggccacgat gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta
     1741 ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc
     1801 ctgaggtttc agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag
     1861 ttattgctca gcggtggcag cagcctaggt taattagccg ctgctgtggt gatgatggtg
     1921 atggcttccg ccgctggttt tctcgccgtt acgatagtta ccgtacaggg tgtggatggt
     1981 gccgtcgtga cgaaacagga acagacgggt gctgctcgga atgttatcca gttcgttctc
     2041 gttgttacgt tcataacggc tgttacggct gtaacggtca acatccactt ccaggatcag
     2101 gtccatgttg aaggtttcca gcgcgctctt aataaagtgg atgctcgcgc acagacgata
     2161 accgtggctc ttctcctcat cgttcttgcc gctcaggtga ccccaaatgc tgctgatcat
     2221 cgccggctca acgtcgctcg ggctgtacca cacacgatgg tctttatcgg tggtcagctt
     2281 catcgcatcg ataatgaagc tcgccggttc cgggatcgga aaacgcacgt tgcccgccca
     2341 cgggtcgaac tcatcaatac cgcaatattc cgcaatgtct ttgatccagc ccttcagttg
     2401 gtagtgcgcg tgatcgatct ccaggttgtc acccgcatcc ggaattttgt agtcataaac
     2461 gttcttggtg gtctgcagcg cacgcagcag gctcaggcta cgctccgggc taaccagcgc
     2521 gctgtgcaca ataatgcttt ccttacggtc gctgcacaca tcgctccaac ggccccacag
     2581 ggtcagcaga cccgggctcg ggcacagggt ctcgttgaaa acgttttcgc tgatgctcgc
     2641 cagccactcg tcacggttat cgctgctgct gttcagccag ctgctacgtt ctttcggcgg
     2701 gatgtcacga cgatccgcca gccaacggtg gtcgttacgg ctaatatcgt gacgacgcag
     2761 ccagtcttga aaaacatctt ccacatcgtc ataatcgcta ccaaccagcg gcttggtcgc
     2821 cagcagctga cccgcggtca tgaacatcgc gtgatagctc aggtaaaagt gataatcgtc
     2881 aacacgcggg tagctgccgt ggctgtggtg gttgtcacga tcctgatagt aacgacgttt
     2941 gttacgttcg tcctcatccc agttacggct gcccttgaaa cccagcacgt cacggataat
     3001 acgcagcatt tccggctcca gttgtttctg gctaacgcca aagcaacgac ccagcggctt
     3061 cagccaatac gggccaaagt cgataccgaa ggtgtagcta tcctcaccac ggtggtcttc
     3121 aactttatcc agcaccggca gggtggtgct ttggttaatg ttacgcagct tgttctcttc
     3181 ctgcgccggg atgctaatca ggtcgctatc gtgcagcgcc agcagggtac gcgccgcaaa
     3241 gtgacgaatc agcacgtgcg gttgatcggt ggtcgcgtag tgataaaagt agccaatgtt
     3301 cgggatcagg cttttaccat catccagcgc aacacgcgcc gccgcaatca tcagccacag
     3361 ctgcgcatgc agggtgtaga acggcaggtt acggtcgcaa aacggcaggg tggtcgcgtt
     3421 aatcgcgtgt tggaaaatgc cctggatcac gcaggtacgg ctcatacggc acagcgccag
     3481 aaccgcatgc gccgcttgcc aacgcatttc cgcttccggg ctacccagac gcgcccaaat
     3541 ataacccgcc aggctgtcct ccacatgggt cggcgggctc agtttttcgt tccacgggcc
     3601 gtcaccatcc tcgtccttca gcacttcgtc gaacaggtcc agcgcgtagc tcagaacatc
     3661 cagcgcctcg ttgctttcca gcttgctaac cagcagaccc ggcaggctga acagacggtc
     3721 gctgttcgcc ggctccgggc tttccgcaat cgcctccagg gtaatgccaa agatttcttt
     3781 ctcgctaata ccgctcagac ggctcgccag gctaaacggg aagatctcat aaacacggct
     3841 cttacgaata cgcatgcaga aacgctggca gtattctttg atcaggcccg ccagggtggt
     3901 cttaatgctc agacggctgg tccactcgtc cggaatgctt tccaggatat atttgaaatc
     3961 gtacaggccc cagtgaaaaa tcgcaccgat cgcggtaatg aagctgcact ctttaccggt
     4021 cttcacacgg ctaatcgctt tcttgatgaa ggtctctttg ctataaaatt ccggaacgtt
     4081 acggaacttt tcgtacgccg cgctgatgcc gtcaatgctg ctcagatcgc agtccttaaa
     4141 gatgctttcc cactcttggt cgtgaccctg ctcgttgtca ttgctgctca ggctgctttt
     4201 cttgctcacg gtttcggtgt gctgcaggcc gctaatacgc tctttcagtt cggtgtgctc
     4261 gatacccagg ctggtgctaa tcgcatccag tttcttcagg ttttggatgt tctgcacgtt
     4321 gaacttggta tagtggtaaa ccacctcaaa cgccataatt ttgtccttat cgtcggtgca
     4381 gctgctcaga acgctgtcca gcagatcgca tttgtgccag tcgttctcga aggtgatcag
     4441 cggcagcgca tccagcgcgt taatcttgtt tttcttaacc aggtgttcaa tggtccacgc
     4501 caggatgcta cggtggttac cgaaggtacg atcacgccaa cggctgataa tcgccagcgc
     4561 gctgctcggg cacagttccg ccaggatctc cacggtgtcg ctccacgcaa agtgtttatc
     4621 acggtcaaca tattcacggg tcagctccgc gcaacgcgcc agtttgtagc tgatctccgg
     4681 cggaacctgg gtcttgcccg ccacatattc cgccaggtcc agaatcgcct cccaacggct
     4741 caggttttca tcacccagtt tgttggtcac ctcgatcgct tggttgaaat attccttcgc
     4801 ttccggctcg tccaggctaa tcaggctacg gctcaggtcg atgtaaccat ccgccttaat
     4861 ctgcgcgtcg ctgtgctcat cacgccacag gctcagcgcc agttccgcaa agtgatagct
     4921 cagctcgccc agaccgctga tttccgcgca aacgctgctg aaacggtgca gggtcggggt
     4981 aaacacacgg ttgcctttgt gttggctcca cttgataatg ttctcaacat cgtctttgct
     5041 cacgttaccc gcttcgatca gaatgtcgaa ccaaacgttc gcgatctcgt tgctgctcag
     5101 gctgtgttcg ctgtagctgt ggcctttaat cgcggtgctc tccttctggg tgtcgctcag
     5161 ttcgctctcc agatccgctt tacgggtctt gcccaggata accttcgccc acaggttata
     5221 ccacggaatc agcacgccgc tgtattgttt cagctgacgc agatcgtcgc tttcaccgtg
     5281 acgcttttcc gccatcagct ctttcttcac ttcggtgctc gccaggtcgc tcagcgccag
     5341 ttggctaccc atcaggttcg cctgcagcgc atacgcacga acgtacgcca cacgctcttt
     5401 gctatattcg ctggtcagcg cgtacggcgg aaccttcggc aggtaacgat ccagcagttg
     5461 gatgctttcg ctctcggtgc aaacgctctg aatcagcgcc atctccacca taccggtgat
     5521 cgccgcaatg gtctggttgt tcgcgtggtt acggtctttg atgctcacac gttggctctt
     5581 cagcagacgc caggtacgac gaatcgcctg ctccgcaacc ggacggtgca gtttacgcgc
     5641 ttccagaacg atgcccatca ccaggctaat atcgttaccc gccgcgatcg ccagttggtc
     5701 cagctcatcg taacgcgcat gcgccagcag ctgcatcgca acaattttgc ccgcgtcgaa
     5761 gctcagctta cgcggacgcc aacgacgcag ctcacgcgcc gccgcttccg caccatgaat
     5821 gttcaggcac gcgatcagca taaccgcacg atcttggtcg gtcacgttct gacggctacg
     5881 ttcatcgtcc ggcagttggc tccagttggt cagccactcc atggtcagac gcagacggct
     5941 acgcgcttca ccgctcagtt ccgggtactc gctcaggatc gccgcatagt acgcgttacg
     6001 gctacccggc caaccggtat ccgggaacgc gttacggcta accagctcct gcacgccgtt
     6061 gctacccaca aatttcgccg ccaggtcaat gttatcacgc agcagaacac gttgacggtt
     6121 gtcacccgcg cattcgccac ccgccttcag cgccagcttc gccgcatcct gataacgacc
     6181 ggtacgcagc gccgccttca gcgcaaattg cagacgctgc agctcgatgt cacgacgttc
     6241 caccgcgctg gtctccggca gcgcctggct gctaatcgcc agttcgatca gctcgctcag
     6301 ctggttaccc tccagcatca gcgccggcag aacgctcgcc gcatagctgc tgtctttggt
     6361 cagcggacgc agcttggtga tgaactggtg cagatccgcc gcgctcggac gaaaacgacg
     6421 ttggaaccag gtttccgccg gctcgtcaaa gaactgaatg gtttcgccgc tcacgatcag
     6481 cggacgaccc agatccagcg caaagctttt gatcgcgctg cccgcaacac cgctaatcgc
     6541 gctcagcacg ctcagcggaa tcagcggacg caggatcgcc agcgcgctgc agatggtgtc
     6601 aatctgcgca cgttcgctga tacccgcggt atcacgcaga cgcgcgatgc tcttctccag
     6661 aacctcgcca atggtgtctt ccacggtctt cgggttcgga cccagcagac gcagggtatc
     6721 gttcagcggc aggttttggc tcaggctcag cgcctgcaca cgcgggttgc agctgctcag
     6781 acggtggaat tcatcaacgt cgctctcgct cgcgtccgga aacttttggt gcaggtgcgc
     6841 cgcggtctca tcacggttga aggtctgcag gctcagggtc agcgcctccg gcggcgggtc
     6901 cagcagttca cgacggtacg gacggcacag cgcaaccagg cacacaccat ccggcagttt
     6961 ctcacgaatc agatccttga taaagctacg ggtttcaccg atttcctccg ccgccatctg
     7021 cgcgttgtcc gccgcatcga taatgatgca cagaaccgcc agcggctcgc tcgcacgcag
     7081 aatgctaatg ctttggctca gacggtgcag gaacgcacgc atatactggc tgatgccggt
     7141 acccgcgttc ggaatcagcg ggtggcacag accacggctc gccatttcgt tcgccatttg
     7201 caccagcgcg gtacggtggt ggtgacgata gctgctcgcg ttacggtact ggcccagacc
     7261 aaagcaatcg tacaggatgc taacgctgcc ggtcggcagg tgttcctcaa tgtgggtgct
     7321 gaagatgctt ttgccaacac ccgcgtccgc gtgaatgatc accggcgcac caaacgcctc
     7381 aaccacacgt tgaaccaggg tcgcttcctg ggtacggctc acgctgtttt cgatacgctc
     7441 aatacggcac ggcgccggga acaggtcggt ctcatcaaca cccagcgcac gcagcacgtc
     7501 catacgggtg atgctcgggt tcgccgcgct ttcggtcagc gctttacggt tcaccagctc
     7561 tttcagcttc agcggcgctt cggtatccag gtccggcagg tagcccgcgc tctcacgcag
     7621 cagaatgtta cgttggctcc acagatcgtc ttgctgaccc tcgatgtgca gcagttggca
     7681 gaaaatgctc agctccgcgc cctgcagacc ggtaaatttt tccagcttcg ccaggtcgtg
     7741 cgggtgacgg gtcacatgtt ggttcgccgc atcgttgatc gcctcgctaa agctgctgct
     7801 aaccggacgg ttggtcacaa accagaactc cagcttggta cgcagggttt caaccgggat
     7861 tttttgaatc agcgccttat agcgggtcgc gaagccttca atggttttct gcagaccgct
     7921 cggcggaaac ggggtgtcgc tatgcatggt gctgtgcttc agttgcatgt aacgaacggt
     7981 ggtcgcggtc gccagctcgt tgctaccata gtattccgcg atgtcaatca gttcctcgcc
     8041 atcctcaacc accggctggc tacccatctc ggtggtgctc gcaccttcga tggtcagcgc
     8101 caccagggtg ctttgcggtt ccagcagacg cagcgcacga cgcgccgccc acaggtagtg
     8161 gaactgatca ccgtcacggc tggtacgaac cagcaggctg tcgctcatgg tatatctcct
     8221 tattaaagtt aaacaaaatt atttctacag gggaattgtt atccgctcac aattccccta
     8281 tagtgagtcg tattaatttc ctaatgcagg agtcgcataa gggagagcgt cgagatcccg
     8341 gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
     8401 caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
     8461 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
     8521 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
     8581 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
     8641 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
     8701 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
     8761 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
     8821 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
     8881 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
     8941 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
     9001 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
     9061 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
     9121 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
     9181 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggaca tctcggtagt gggatacgac
     9241 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
     9301 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     9361 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     9421 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     9481 cgactggaaa gcgggcagtg agcgcaacgc aattaatgta agttagctca ctcattaggc
     9541 accgggatct cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     9601 gcggggcatg actaacatga gaattacaac ttatatcgta tggggctgac ttcaggtgct
     9661 acatttgaag agataaattg cactgaaatc tagagcggtt cagtagaaaa gatcaaagga
     9721 tcttcttgag atcctttttt tctgcgcgta atcttttgcc ctgtaaacga aaaaaccacc
     9781 tggggaggtg gtttgatcga agg
//
