LOCUS       sequence-370040-        9264 bp    DNA     circular SYN 14-APR-2025
DEFINITION  FXR - Farnesoid X receptor gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9264)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE12
FEATURES             Location/Qualifiers
     source          1..9264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2096..2127
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2154..2163
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2160..3809
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3813..3932
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    4024..4612
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4077..4097)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4495..4506)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4627..4943
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4794..4929
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4994..4996,4997..4999,5000..5713)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5039..5060)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5300..5319)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5647..5668
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5785..6018
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6090..6211
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6127..6146)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6181..6200
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6251..6386
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6406..6425)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6407..6425)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6435..6452)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6435..6451)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6444..6466)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6593..7048
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6680..6699)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6890..6911
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7074..7178
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7179..7247,7248..8039)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7397..7416)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8210..8798
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8699..8718
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8952..8969
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9086..9107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9122..9127,9128..9145,9146..9152)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9160..9176
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9165..9187
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9184..9200
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9184..9200
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9219..9239
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9221..9239
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcaaact gagggtcagt gacccaagtg aaaaactgag
     1981 ggtcagtgac ccaagtgaaa aactgagggt cagtgaccca agtgaaaaac tgagggtcag
     2041 tgacccaagt gaagatccga gaacagatct ggcctcggcg gccaagctta gacactagag
     2101 ggtatataat ggaagctcga cttccagctt ggcaatccgg tactgttggt aaagccacca
     2161 tggaagatgc caaaaacatt aagaagggcc cagcgccatt ctacccactc gaagacggga
     2221 ccgccggcga gcagctgcac aaagccatga agcgctacgc cctggtgccc ggcaccatcg
     2281 cctttaccga cgcacatatc gaggtggaca ttacctacgc cgagtacttc gagatgagcg
     2341 ttcggctggc agaagctatg aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt
     2401 gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg
     2461 ctgtggcccc agctaacgac atctacaacg agcgcgagct gctgaacagc atgggcatca
     2521 gccagcccac cgtcgtattc gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa
     2581 agaagctacc gatcatacaa aagatcatca tcatggatag caagaccgac taccagggct
     2641 tccaaagcat gtacaccttc gtgacttccc atttgccacc cggcttcaac gagtacgact
     2701 tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct gatcatgaac agtagtggca
     2761 gtaccggatt gcccaagggc gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc
     2821 atgcccgcga ccccatcttc ggcaaccaga tcatccccga caccgctatc ctcagcgtgg
     2881 tgccatttca ccacggcttc ggcatgttca ccacgctggg ctacttgatc tgcggctttc
     2941 gggtcgtgct catgtaccgc ttcgaggagg agctattctt gcgcagcttg caagactata
     3001 agattcaatc tgccctgctg gtgcccacac tatttagctt cttcgctaag agcactctca
     3061 tcgacaagta cgacctaagc aacttgcacg agatcgccag cggcggggcg ccgctcagca
     3121 aggaggtagg tgaggccgtg gccaaacgct tccacctacc aggcatccgc cagggctacg
     3181 gcctgacaga aacaaccagc gccattctga tcacccccga aggggacgac aagcctggcg
     3241 cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt ggacttggac accggtaaga
     3301 cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct
     3361 acgttaacaa ccccgaggct acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg
     3421 gcgacatcgc ctactgggac gaggacgagc acttcttcat cgtggaccgg ctgaagagcc
     3481 tgatcaaata caagggctac caggtagccc cagccgaact ggagagcatc ctgctgcaac
     3541 accccaacat cttcgacgcc ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc
     3601 ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac cgagaaggag atcgtggact
     3661 atgtggccag ccaggttaca accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg
     3721 aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa gatccgcgag attctcatta
     3781 aggccaagaa gggcggcaag atcgccgtga attctcacgg cttccctccc gaggtggagg
     3841 agcaggccgc cggcaccctg cccatgagct gcgcccagga gagcggcatg gatagacacc
     3901 ctgctgcttg cgccagcgcc aggatcaacg tctaaggccg cgactctaga acgcgtcact
     3961 gcggctaaga gcggggtgtc caagactaca gacatcagcg cgtaccggtt agtaatgatc
     4021 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4081 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4141 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4201 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4261 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4321 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4381 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4441 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4501 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4561 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggcgat
     4621 ggtaccggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4681 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4741 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4801 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4861 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4921 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     4981 atcagcacct agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     5041 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     5101 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     5161 gccctggccc accctcgtga ccaccttcac ctacggcgtg cagtgcttcg cccgctaccc
     5221 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     5281 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     5341 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     5401 catcctgggg cacaagctgg agtacaacta caacagccac aaggtctata tcaccgccga
     5461 caagcagaag aacggcatca aggtgaactt caagacccgc cacaacatcg aggacggcag
     5521 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     5581 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     5641 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     5701 gctgtacaag taaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     5761 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     5821 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     5881 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     5941 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6001 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     6061 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     6121 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6181 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     6241 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     6301 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     6361 gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg
     6421 tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     6481 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     6541 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     6601 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     6661 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     6721 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     6781 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     6841 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     6901 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6961 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     7021 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     7081 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7141 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7201 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7261 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     7321 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     7381 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     7441 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     7501 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     7561 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     7621 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     7681 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     7741 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     7801 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     7861 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     7921 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     7981 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8041 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8101 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8161 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8221 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8281 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8341 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8401 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8461 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8521 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8581 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8641 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8701 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8761 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8821 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8881 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8941 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9001 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9061 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     9121 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     9181 acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg
     9241 aacaaaagct ggagctgcaa gctt
//
