LOCUS       sequence-370473-       12125 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expresses beta-galactosidase by D-mannose promoter for Bacillus 
            subtilis. There is a secretion peptide (PhoD) and nuclear 
            localization signal (SV40) that is fused to beta-galactosidase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12125)
  AUTHORS   Madsen CS, Makela AV, Greeson EM, Hardy JW, Contag CH
  TITLE     Engineered endosymbionts that alter mammalian cell surface marker, 
            cytokine and chemokine expression.
  JOURNAL   Commun Biol. 2022 Aug 30;5(1):888. doi: 10.1038/s42003-022-03851-6.
  PUBMED    36042261
REFERENCE   2  (bases 1 to 12125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2022-08-30"; volume: "5(1):888. doi"; pages: " 
            10.1038/s42003-022-03851-6"
COMMENT     Sequence Label: pDR111_mannose_Bgal NLS
FEATURES             Location/Qualifiers
     source          1..12125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3120..6191)
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="TMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTD
                     RPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVT
                     YPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQ
                     DSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQI
                     SDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDE
                     RGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENG
                     LLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWY
                     TLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSL
                     GNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPK
                     WSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLI
                     KYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQT
                     IEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQL
                     WLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGN
                     KRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKA
                     AGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVE
                     VASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYV
                     FPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGF
                     HMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     6143..6165
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6157..6174
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6158..6174
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(6192..6212)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      complement(6541..6575)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(6655..6682)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(6701..6787)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(10296..10884)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10376..10395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11055..11846,11847..11915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11678..11697
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11916..12020)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     12088..12106
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcgatggg atgtcacgca gaattaaata
      661 gtacataatg gatttcctta cgcgaaatac gggcagacat ggcctgcccg gttattatta
      721 tttttgacac cagaccaact ggtaatggta gcgaccggcg ctcaggatcc taactcatta
      781 ataatccaaa tgagataaaa atgtcatgac attggtttct ttcgtcagct tctggacgaa
      841 ggccggctgc tcgctaagat atgaaagctc gctaaacagc tgctttgtca tagtttgatc
      901 ctcgtgtttg acagccagca taaaaacgag cgaaactttt tcatttcccc attcaagcgg
      961 ctcctttaat gtggcaatcg cgattgctga ttgtttaata aatttagcgt ttgcatgagg
     1021 aatggcaatg cctgacccaa tatttgtagc cgacattttt tccctcatca ccgcatgaac
     1081 cgcatagtcc ttatcaacat agcctttctc aaacaaagct gtcgccagct gttcgattaa
     1141 cttgtagcga tgctccgcct cctgctgcaa aaaaaccaaa aaaggagtcg tattgttcag
     1201 catctgaaac gttttttgtt tttggcggtg tgattcgccc agctgacgta taaacgcact
     1261 taatttcttc tcttcgccag gctctaaaag cggagacaca acaatatggg gaaccgttat
     1321 attctccagc gcaatggtgg atatgacaag atcaatatcc tcatgctttt tgatataatc
     1381 ctttaaatcg gcctttgcaa tacaagccat tacagcgatt tggtgatatt tccgttcaat
     1441 ttttgtccgc agcaattgtg acatcccaat ccccatgtgg cagacgataa ccgccttttt
     1501 atgtgtctcg ctgctgccct gcatccgttc gatcgctgcc tgaaaatgca atgtcaaata
     1561 tgctgcctct tcctcaggaa tatgaagatc aaaagtttga ttgatgtctt ccaaaacatc
     1621 aatgatgagg tggaacaaat aagggtacat ctttttgatg tcattgagca taggatttgc
     1681 tacagaaaga tcgtagctca gccgttgtag cacagtattg agatgaatgt tcaaaccatt
     1741 gatcaaatcc tgatccttgt gaaaatccat cattttcagc tctgaaacac gattgattaa
     1801 atgccctaca accttaggaa gcacagcatt ttcaaggttc tcttctgtct gcaatggata
     1861 acgaacctta ccgcccaata tatgtaatgt taagtacacg gcttcttctt cagggaagcg
     1921 aattgcaaaa accggttcaa gccgctgtaa acaagcaaat gtccattgat attctttctt
     1981 cttttttact gccgccattt cttttgggga caatgaaatc ggctgcttca ttttgatgcg
     2041 gcggaccatt aacaatgtat gcagcaacaa actttcaaac gtttcgtctg tgaaatagag
     2101 ggaatgcttt ttttgtagtg atttgatttc tttcgtcaca aagtctactt cataatgtaa
     2161 aaacttactt ttaataaatt ggctggtaaa cgctgtattg tgaatcaaat cagatattct
     2221 tgccaatgcc tttcttttat ttctttcgtt cccttcaacc tttaatccaa gccgctgcct
     2281 agaaaccagt gtgagatcaa aacgcttcag ccactcttct actgcgtata aatccttttt
     2341 aatggaagat cgattgacaa aatgccgagc ggcaatgtct tttgcggaaa caggcttcgg
     2401 attcattaat aagtcataag caatgtgcag cattctttcc ttatcgctcc gctgcctaga
     2461 agaaaaatgt tcagtatgta attgttggct tagccaggtc ctttcctgtt cttctatatg
     2521 taaataaacg cccaaccctg gttttcgaat aagctgtgca tgagaatgct cattgagata
     2581 atcctcgatc actttcaaat cattccggat tgttttttca gaacactgta ccctatctgc
     2641 gaaatcttgc acaactaaat agtcatcagg ttcagataac aacagataca aaatctcttt
     2701 ttgtcttgta tttatgtatt ccatctgttt ttctatcctt cctgtcagta tctacttgag
     2761 caaattgata gtataagtat actatataag aaaccgttta cattataaaa caaaatcata
     2821 gagacaaaag tttcgttttt taccgaagct tccggaaaaa agcgattttt atactctttg
     2881 agtttgctat gtaatgtata ttcagcagaa attcataaaa atactccaat tcttcaccta
     2941 tttagaacga agattttcta atgtatgagt acgaaagtaa gaacatgatg aagaaaagtg
     3001 atttggtatc ttcggggata aagtgcaggt aacaaaattt ccacacgctc aacaaactaa
     3061 tacccatcaa atgtgtgcta ttaacatttt aaggatggtc attctctctc agcaatcggt
     3121 tatttttgac accagaccaa ctggtaatgg tagcgaccgg cgctcagctg taattccgcc
     3181 gatactgacg ggctccagga gtcgtcgcca ccaatcccca tatggaaacc gtcgatattc
     3241 agccatgtgc cttcttccgc gtgcagcaga tggcgatggc tggtttccat cagttgctgt
     3301 tgactgtagc ggctgatgtt gaactggaag tcgccgcgcc actggtgtgg gccataattc
     3361 aattcgcgcg tcccgcagcg cagaccgttt tcgctcggga agacgtacgg ggtatacatg
     3421 tctgacaatg gcagatccca gcggtcaaaa caggcggcag taaggcggtc gggatagttt
     3481 tcttgcggcc ctaatccgag ccagtttacc cgctctgcta cctgcgccag ctggcagttc
     3541 aggccaatcc gcgccggatg cggtgtatcg ctcgccactt caacatcaac ggtaatcgcc
     3601 atttgaccac taccatcaat ccggtaggtt ttccggctga taaataaggt tttcccctga
     3661 tgctgccacg cgtgagcggt cgtaatcagc accgcatcag caagtgtatc tgccgtgcac
     3721 tgcaacaacg ctgcttcggc ctggtaatgg cccgccgcct tccagcgttc gacccaggcg
     3781 ttagggtcaa tgcgggtcgc ttcacttacg ccaatgtcgt tatccagcgg tgcacgggtg
     3841 aactgatcgc gcagcggcgt cagcagttgt tttttatcgc caatccacat ctgtgaaaga
     3901 aagcctgact ggcggttaaa ttgccaacgc ttattaccca gctcgatgca aaaatccatt
     3961 tcgctggtgg tcagatgcgg gatggcgtgg gacgcggcgg ggagcgtcac actgaggttt
     4021 tccgccagac gccactgctg ccaggcgctg atgtgcccgg cttctgacca tgcggtcgcg
     4081 ttcggttgca ctacgcgtac tgtgagccag agttgcccgg cgctctccgg ctgcggtagt
     4141 tcaggcagtt caatcaactg tttaccttgt ggagcgacat ccagaggcac ttcaccgctt
     4201 gccagcggct taccatccag cgccaccatc cagtgcagga gctcgttatc gctatgacgg
     4261 aacaggtatt cgctggtcac ttcgatggtt tgcccggata aacggaactg gaaaaactgc
     4321 tgctggtgtt ttgcttccgt cagcgctgga tgcggcgtgc ggtcggcaaa gaccagaccg
     4381 ttcatacaga actggcgatc gttcggcgta tcgccaaaat caccgccgta agccgaccac
     4441 gggttgccgt tttcatcata tttaatcagc gactgatcca cccagtccca gacgaagccg
     4501 ccctgtaaac ggggatactg acgaaacgcc tgccagtatt tagcgaaacc gccaagactg
     4561 ttacccatcg cgtgggcgta ttcgcaaagg atcagcgggc gcgtctctcc aggtagcgaa
     4621 agccattttt tgatggacca tttcggcaca gccgggaagg gctggtcttc atccacgcgc
     4681 gcgtacatcg ggcaaataat atcggtggcc gtggtgtcgg ctccgccgcc ttcatactgc
     4741 accgggcggg aaggatcgac agatttgatc cagcgataca gcgcgtcgtg attagcgccg
     4801 tggcctgatt cattccccag cgaccagatg atcacactcg ggtgattacg atcgcgctgc
     4861 accattcgcg ttacgcgttc gctcatcgcc ggtagccagc gcggatcatc ggtcagacga
     4921 ttcattggca ccatgccgtg ggtttcaata ttggcttcat ccaccacata caggccgtag
     4981 cggtcgcaca gcgtgtacca cagcggatgg ttcggataat gcgaacagcg cacggcgtta
     5041 aagttgttct gcttcatcag caggatatcc tgcaccatcg tctgctcatc catgacctga
     5101 ccatgcagag gatgatgctc gtgacggtta acgcctcgaa tcagcaacgg cttgccgttc
     5161 agcagcagca gaccattttc aatccgcacc tcgcggaaac cgacatcgca ggcttctgct
     5221 tcaatcagcg tgccgtcggc ggtgtgcagt tcaaccaccg cacgatagag attcgggatt
     5281 tcggcgctcc acagtttcgg gttttcgacg ttcagacgta gtgtgacgcg atcggcataa
     5341 ccaccacgct catcgataat ttcaccgccg aaaggcgcgg tgccgctggc gacctgcgtt
     5401 tcaccctgcc ataaagaaac tgttacccgt aggtagtcac gcaactcgcc gcacatctga
     5461 acttcagcct ccagtacagc gcggctgaaa tcatcattaa agcgagtggc aacatggaaa
     5521 tcgctgattt gtgtagtcgg tttatgcagc aacgagacgt cacggaaaat gccgctcatc
     5581 cgccacatat cctgatcttc cagataactg ccgtcactcc agcgcagcac catcaccgcg
     5641 aggcggtttt ctccggcgcg taaaaatgcg ctcaggtcaa attcagacgg caaacgactg
     5701 tcctggccgt aaccgaccca gcgcccgttg caccacagat gaaacgccga gttaacgcca
     5761 tcaaaaataa ttcgcgtctg gccttcctgt agccagcttt catcaacatt aaatgtgagc
     5821 gagtaacaac ccgtcggatt ctccgtggga acaaacggcg gattgaccgt aatgggatag
     5881 gtcacgttgg tgtagatggg cgcatcgtaa ccgtgcatct gccagtttga ggggacgacg
     5941 acagtatcgg cctcaggaag atcgcactcc agccagcttt ccggcaccgc ttctggtgcc
     6001 ggaaaccagg caaagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     6061 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     6121 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaatcc
     6181 gtaatcatgg tcacttttct ttttttttta ggcgctccca ctgattgcgc aattgtcagt
     6241 cccagtgaca gtcccgcaat ttttcccgct ccttgaataa attttcttct gtcaaatgtg
     6301 ttgttttgaa atgattcttc tttcagtttt tgcacccatt cgtcaaatct tgagtcatac
     6361 gccataactt acctccttct gtcgactaag cttaaggaaa cctcctttaa agtgtgaata
     6421 ataagatctt gtctcaactg tataccgaaa tcagctcatt aaaatcgctt tttttaccat
     6481 aggttccggt aataaaggca tttttcccta taacaaaaaa agcaaggaat aatcccctgc
     6541 ataaaaaacg cccggcggca accgagcgtt ctgaattaat taatcatcgg gaagatcttc
     6601 atcaccgaaa cgcggcaggc agctctagag ttaacaagag tttgtagaaa cgcaaaaagg
     6661 ccatccgtca ggatggcctt ctgcttagct agagcggcgg atttgtccta ctcaggagag
     6721 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt
     6781 ttatttgatg cctcaagcta gaggtcgaat tcctgcagct ggcgaatggc gattttcgtt
     6841 cgtgaataca tgttataata actataacta ataacgtaac gtgactggca agagatattt
     6901 ttaaaacaat gaataggttt acacttactt tagttttatg gaaatgaaag atcatatcat
     6961 atataatcta gaataaaatt aactaaaata attattatct agataaaaaa tttagaagcc
     7021 aatgaaatct ataaataaac taaattaagt ttatttaatt aacaactatg gatataaaat
     7081 aggtactaat caaaatagtg aggaggatat atttgaatac atacgaacaa attaataaag
     7141 tgaaaaaaat acttcggaaa catttaaaaa ataaccttat tggtacttac atgtttggat
     7201 caggagttga gagtggacta aaaccaaata gtgatcttga ctttttagtc gtcgtatctg
     7261 aaccattgac agatcaaagt aaagaaatac ttatacaaaa aattagacct atttcaaaaa
     7321 aaataggaga taaaagcaac ttacgatata ttgaattaac aattattatt cagcaagaaa
     7381 tggtaccgtg gaatcatcct cccaaacaag aatttattta tggagaatgg ttacaagagc
     7441 tttatgaaca aggatacatt cctcagaagg aattaaattc agatttaacc ataatgcttt
     7501 accaagcaaa acgaaaaaat aaaagaatat acggaaatta tgacttagag gaattactac
     7561 ctgatattcc attttctgat gtgagaagag ccattatgga ttcgtcagag gaattaatag
     7621 ataattatca ggatgatgaa accaactcta tattaacttt atgccgtatg attttaacta
     7681 tggacacggg taaaatcata ccaaaagata ttgcgggaaa tgcagtggct gaatcttctc
     7741 cattagaaca tagggagaga attttgttag cagttcgtag ttatcttgga gagaatattg
     7801 aatggactaa tgaaaatgta aatttaacta taaactattt aaataacaga ttaaaaaaat
     7861 tataaaaaaa ttgaaaaaat ggtggaaaca cttttttcaa tttttttgtt ttattattta
     7921 atatttggga aatattcatt ctaattggta atcagatttt agaaaacaat aaacccttgc
     7981 atagggggaa tccgtttagg ctgggcggtg atagcttctc gttcaggcag tacgcctctt
     8041 ttcttttcca gacctgaggg aggcggaaat ggtgtgaggt tcccggggaa aagccaaata
     8101 ggcgatcgcg ggagtgcttt atttgaagat caggctatca ctgcggtcaa tagatttcac
     8161 aatgtgatgg ctggacagcc tgaggaactc tcgaacccga atggaaacaa ccagatattt
     8221 atgaatcagc gcggctcaca tggcgttgtg ctggcaaatg caggttcatc ctctgtctct
     8281 atcaatacgg caacaaaatt gcctgatggc aggtatgaca ataaagctgg agcgggttca
     8341 tttcaagtga acgatggtaa actgacaggc acgatcaatg ccaggtctgt agctgtgctt
     8401 tatcctgatg atattgcaaa agcgcctcat gttttccttg agaattacaa aacaggtgta
     8461 acacattctt tcaatgatca actgacgatt accttgcgtg cagatgcgaa tacaacaaaa
     8521 gccgtttatc aaatcaataa tggaccagag acggcgttta aggatggaga tcaattcaca
     8581 atcggaaaag gagatccatt tggcaaaaca tacaccatca tgttaaaagg aacgaacagt
     8641 gatggtgtaa cgaggaccga gaaatacagt tttgttaaaa gagatccagc gtcggccaaa
     8701 accatcggct atcaaaatcc gaatcattgg agccaggtaa atgcttatat ctataaacat
     8761 gatgggagcc gagtaattga attgaccgga tcttggcctg gaaaaccaat gactaaaaat
     8821 gcagacggaa tttacacgct gacgctgcct gcggacacgg atacaaccaa cgcaaaagtg
     8881 atttttaata atggcagcgc ccaagtgccc ggtcagaatc agcctggctt tgattacgtg
     8941 ctaaatggtt tatataatga ctcgggctta agcggttctc ttccccattg agggcaaggc
     9001 tagacgggac ttaccgaaag aaaccatcaa tgatggtttc ttttttgttc ataaatcaga
     9061 caaaactttt ctcttgcaaa agtttgtgaa gtgttgcaca atataaatgt gaaatacttc
     9121 acaaacaaaa agacatcaaa gagaaacata ccctgcaagg atgctgatat tgtctgcatt
     9181 tgcgccggag caaaccaaaa acctggtgag acacgccttg aattagtaga aaagaacttg
     9241 aagattttca aaggcatcgt tagtgaagtc atggcgagcg gatttgacgg cattttctta
     9301 gtcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc
     9361 gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag
     9421 cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc
     9481 acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc
     9541 gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctac
     9601 tgaagtggat ttctttaaga gctcctttaa cttcctcacc agtagttgta tcggtaccat
     9661 aagtagaagc agcaacccaa gtagctttac cagcatccgg ttcaaccagc atagtaagaa
     9721 tcttactgga catcggcagt tcttcgaaca gtgcgccaac taccagctct ttctgcagtt
     9781 cattcagggc accggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     9841 tcatcctgtc tcttgatcca tggattacgc gttaacccgg gcccgcggat gcatatgatc
     9901 agatcttaag gcctaggtct agagtctttg ttttgacgcc attagcgtac gtaacaatcc
     9961 tcgttaaagg acaaggacct gagcggaagt gtatcgtaca gtagacggag tatactagta
    10021 tagtctatag tccgtggaat tattatattt atctccgacg atattctcat cagtgaaatc
    10081 cagctggagt tctttagcaa atttttttat tagctgaact tagtattagt ggccatactc
    10141 ctccaatcca aagctattta gaaagattac tatatcctca aacaggcggt aaccggcctc
    10201 ttcatcggga atgcgcgcga ccttcagcat cgccggcatg tccccctggc ggacgggaag
    10261 tatccagctc gaggtcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
    10321 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
    10381 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
    10441 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
    10501 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
    10561 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
    10621 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
    10681 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
    10741 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
    10801 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
    10861 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
    10921 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
    10981 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
    11041 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
    11101 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
    11161 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
    11221 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
    11281 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
    11341 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
    11401 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
    11461 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
    11521 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
    11581 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
    11641 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
    11701 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
    11761 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
    11821 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
    11881 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
    11941 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
    12001 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
    12061 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
    12121 gaatt
//
