LOCUS       sequence-370841-       11381 bp    DNA     circular SYN 15-APR-2025
DEFINITION  In vivo gene amplification (HamAmp) of limonene synthetic module at 
            RPL25 locus (23 copy). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11381)
  TITLE     Vickers Lab plasmids
REFERENCE   2  (bases 1 to 11381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pIT6EG7mlh
FEATURES             Location/Qualifiers
     source          1..11381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(745..761)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(745..761)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(758..780)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    769..785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(793..799,800..817,818..823))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    838..859
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(976..993)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1147..1735)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1227..1246)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1906..2697,2698..2766))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2529..2548
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2767..2871)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2939..2957
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2995..3017)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3117..3136
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3330..3352
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3344..3361
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3345..3361
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6733..7107
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
     CDS             complement(9950..9976)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
ORIGIN
        1 gtcgtaatga tttgcatgcc cgccatctct actcactcca taatattaca tatagatata
       61 ggacaagccc gcattttcat actgaaaggt aaacttctat tattatagtg gtatccaacg
      121 ttcaccgctt ccagcatagc agaaattacg tgtttttgca tatgttatgc tgatcattgt
      181 atgcttacta ccatttttct ttgcttcgcc ttgccttctt tgacgttttt ttgaagcaaa
      241 aaaaaagtca agacagatgt gcttacaaaa ccatgtaagg ctcattttca aagaagctac
      301 taatagaaag agaacaaagc gtttacgagt ctggaaaatc aatggctcca tctggtatgt
      361 gaactgcaat attaatagca cgagaaaatt gagaggaaga tagatgggaa ctagtagagt
      421 tgatattgat gagatacgaa aaccacacgt aaataaacta tccgacgaca agaatagtgc
      481 attaaggact tgattaagat atggtgagca acgtaattat cgggctcaac agtttattag
      541 caatcgtttt gatagaagcg ttgatgctgt ggaagttgtt ctttttacta ccaacagcca
      601 ttaacaaatc cattagagtg ttcgttcgtt tttctgctca gattcaagaa aattattcca
      661 tcctcattat acttttttct tctatttcgt gctccacgtc gaggtatcaa ggaacatagt
      721 ttaaacaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
      781 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
      841 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
      901 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      961 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     1021 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1081 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1141 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1201 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1261 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1321 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1381 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1441 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1501 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     1561 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1621 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1681 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1741 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1801 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1861 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1921 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1981 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2041 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     2101 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     2161 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     2221 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     2281 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     2341 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     2401 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     2461 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     2521 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     2581 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     2641 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     2701 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2761 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2881 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     2941 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     3001 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     3061 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     3121 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     3181 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcacaact gttgggaagg
     3241 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     3301 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     3361 tgaattcagt ttaaacatgt actaatcagt ctaacacccc ggaggaggct tatcattaca
     3421 tattcttgtc tcggcgttct ttaagtttct tatcttgtat gcccgatata gcaaccttgt
     3481 tggtaccaat ctaacggttt ccgtactttg caatgaagag atgaggaggc atgggtcact
     3541 tatttaatat gtacgggtgt ttacatggag ttgctttctt tttttgtctc agcagtcatt
     3601 gtgcgccaaa aaaagagaaa accgtgagcc gaagtccacg ctctggagtt aggctctccc
     3661 attacggaga gaagcatttc ctcagcctgg gagccccgtt ggaacagtca ggctaaactg
     3721 ggccttccta cccactgctt gctgtttctc actggacgca caaggggatt ttctttctac
     3781 cttcggcttg cctcactgcg ttggggcttc ccaatgcaac ttcgttcgta tgcatacaat
     3841 cttttagata ttatctttta aaattatttt aaaacaattt taaatgtatc tcatatgctt
     3901 ttcttctgct gttgaaaagg ctaaacaaag aagatcaata agataaagcg gccgcaaaat
     3961 gtctaagaat atcgttgtcc taccgggtga tcacgtcggt aaagaagtta ctgacgaagc
     4021 tattaaggtc ttgaatgcca ttgctgaagt ccgtccagaa attaagttca atttccaaca
     4081 tcacttgatc gggggtgctg ccatcgatgc cactggcact cctttaccag atgaagctct
     4141 agaagcctct aagaaagccg atgctgtctt actaggtgct gttggtggtc caaaatgggg
     4201 tacgggcgca gttagaccag aacaaggtct attgaagatc agaaaggaat tgggtctata
     4261 cgccaacttg agaccatgta actttgcttc tgattcttta ctagatcttt ctcctttgaa
     4321 gcctgaatat gcaaagggta ccgatttcgt cgtcgttaga gaattggttg gtggtatcta
     4381 ctttggtgaa agaaaagaag atgaaggtga cggagttgct tgggactctg agaaatacag
     4441 tgttcctgaa gttcaaagaa ttacaagaat ggctgctttc ttggcattgc aacaaaaccc
     4501 accattacca atctggtctc ttgacaaggc taacgtgctt gcctcttcca gattgtggag
     4561 aaagactgtt gaagaaacca tcaagactga gttcccacaa ttaactgttc agcaccaatt
     4621 gatcgactct gctgctatga ttttggttaa atcaccaact aagctaaacg gtgttgttat
     4681 taccaacaac atgtttggtg atattatctc cgatgaagcc tctgttattc caggttcttt
     4741 gggtttatta ccttctgcat ctctagcttc cctacctgac actaacaagg cattcggttt
     4801 gtacgaacca tgtcatggtt ctgccccaga tttaccagca aacaaggtta acccaattgc
     4861 taccatctta tctgcagcta tgatgttgaa gttatccttg gatttggttg aagaaggtag
     4921 ggctcttgaa gaagctgtta gaaatgtctt ggatgcaggt gtcagaaccg gtgaccttgg
     4981 tggttctaac tctaccactg aggttggcga tgctatcgcc aaggctgtca aggaaatctt
     5041 ggcttaaaga gtcttttgta acgaccccgt ctccaccaac ttggtatgct tgaaatctca
     5101 aggccattac acattcagtt atgtgaacga aaggtcttta tttaacgtag cataaactaa
     5161 ataatacagg ttccggttag cctgcgggcc caaagctagc tctaattggt ttaattaata
     5221 aatttaatat tatttttaaa tttttcttta aatatacaat aaatctttca taacatgtta
     5281 aattcatgat taagcgtaaa taaagtgtag tggcagagtg cacggggttt cctgtgcctt
     5341 acaaagtagg taccaatttg cgtattgcag cgagggttcc ggttactatt tataattacg
     5401 tgttagtgta ctgtgatttt attgaggcta taacaagaaa aggatctgtg aaggttttga
     5461 gagaatgagg cgaaaacaca gggttatttt catcagcaac gccgctgttt cagcggcacc
     5521 ctgccctttt cttttcgcaa gatgagagta aagagttgta catcaggtaa gaatgttatt
     5581 atttaaattc gaagtgataa attcttttca tgatgaatca ctcgcttata tggggtagaa
     5641 tatatatata tgtgtgtgtg tgtgtgtgtt tgtgtatgta gggatggtgc gcgtttgttg
     5701 tgtgacattt gctactcatt cttttccttt tcctacgact ggcttaacgg gaatattatc
     5761 aatttgctgc attcttatgc ttcggtccga tgctcaagct taacttcttc gtgaggaagg
     5821 aaagtgttgt ctcctacttt tttcaaattt tcgaattgta tttatattta tttagtactt
     5881 cttgagttta catatccttc gtaaaaatgc aacttttgtc gaaaaacact tccaaaaaaa
     5941 aataataatg aatttatgaa gcatactaac gagcgagcac atcgctgagg tatcattact
     6001 tcatgagata aattaactcg agtaaaccaa ttttatttga acttgccccg ctaatcgtac
     6061 agacgtacca gatccaggtc ataacggtgc cattagaagg ccgtagaata gacccactaa
     6121 agctatcaca ggcaaccttg catccaagtc taattaaacg gagcagaatc tgttccagta
     6181 atgtataaag aattaactac cggaaaagcg tcttccgggg cggctcaacc ggattacatt
     6241 cttttttctt ttttgcggcc agcattcgag tcacgtgctc aaatactgag gaaggggttc
     6301 ttccggtagg caccggcgct cactcttccg tgcggcacac tccccgcagt tcaggggcca
     6361 tgtgcctgtg agagtatttc cgcaggccat agttccgggg tggaccactc cgagctaatg
     6421 ctaaagtaag cccttcccaa cttgaaatag gaacaaaagt caattatgct cctcctgtaa
     6481 agctaaacgt gtaaagattt tagagtgaga cgttgttagc agtcgttgtt caagggaggg
     6541 atgagttgag gaataaaatg cggaaaaagt gtgggttaga cagacatata taaactctag
     6601 ccattcggct gaaatatatc tgagttcatt tgtgataact tttttgatta cttgttcata
     6661 tatccacaag aacaacgact attaaacaat agaaaatagt ataataaaaa aagtttttac
     6721 agtggatcca aaatggaaca cgttgctttc ggttctgaag atattgaaaa cactttggct
     6781 aaaatggatg atggtcaatt agacggttta gctttcggtg ctattcaatt ggacggtgat
     6841 ggtaacattt tgcaatacaa tgctgctgaa ggtgatatta ctggtagaga tccaaaacaa
     6901 gttattggta agaacttctt taaggacgtt gctccaggta ctgactctcc agagttctat
     6961 ggtaagttta aggaaggtgt tgcttctggt aacttaaaca ctatgtttga atggatgatc
     7021 ccaacttcca gaggtccaac taaggttaaa gttcacatga aaaaggcttt atctggtgac
     7081 tcttactggg ttttcgttaa aagagttggt gctactaact tctctttgtt gaagttggct
     7141 ggtgacgttg aacttaaccc aggtccaaaa attgaagaag gtaagttggt tatctggatt
     7201 aacggtgaca aaggttacaa cggtttagcc gaagtcggta agaagtttga aaaggatact
     7261 ggtatcaaag tcaccgtcga acacccagac aaattagaag aaaaattccc acaagttgct
     7321 gctactggtg acggtccaga tattatcttt tgggctcacg atagatttgg tggttacgcc
     7381 caatctggtt tgttagctga aattacccca gataaggctt tccaagataa gttgtaccca
     7441 ttcacttggg atgctgtcag atacaacggt aagttgattg cttacccaat tgctgttgaa
     7501 gccttgtctt tgatctataa caaggatttg ttacctaatc caccaaaaac ttgggaagaa
     7561 atcccagctt tggataaaga attgaaagct aaaggtaaat ccgctttgat gttcaactta
     7621 caagaaccat acttcacttg gcctttaatc gctgctgacg gtggttacgc ttttaagtac
     7681 gaaaatggta aatacgatat taaggacgtt ggtgttgaca acgccggtgc taaagctggt
     7741 ttgactttct tggtcgattt gattaaaaac aagcatatga atgctgacac cgattactcc
     7801 atcgctgaag ctgctttcaa caaaggtgaa actgctatga ccatcaacgg tccttgggcc
     7861 tggtccaaca ttgacacttc taaggtcaac tatggtgtta ctgtcttgcc tacctttaag
     7921 ggtcaaccat ccaaaccatt tgttggtgtt ttgtctgctg gtatcaacgc tgcttctcca
     7981 aataaggaat tggctaagga atttttggaa aactacttgt taactgacga aggtttggaa
     8041 gccgttaaca aagataaacc attgggtgct gtcgccttga aatcttacga agaagaattg
     8101 gctaaagatc caagaattgc tgctactatg gaaaacgctc aaaagggtga aatcatgcct
     8161 aatattccac aaatgtctgc tttctggtat gccgtccgta ctgctgtcat caacgccgct
     8221 tccggtagac aaactgttga cgaagccttg aaggatgccc aaactagaat cactaagcca
     8281 ggttctggta gaagatcagc taactatcaa ccatccattt gggaccacga ctttttacaa
     8341 tccttgaact ctaactacac cgacgaagca tacaagagaa gagcagaaga attacgtggt
     8401 aaagtaaaga tagccatcaa agatgtcatc gaacctttgg accaattgga attgattgat
     8461 aacttgcaaa gattgggttt agcccataga tttgaaaccg aaatcagaaa catcttgaac
     8521 aacatctata acaacaataa ggattacaac tggagaaagg aaaatttgta cgctacttcc
     8581 ttggaattca gattgttaag acaacacggt tacccagtca gtcaagaagt ttttaacggt
     8641 ttcaaggatg accaaggtgg ttttatttgt gatgacttca agggtatatt gtccttgcat
     8701 gaagcttctt actactcatt ggaaggtgaa agtataatgg aagaagcatg gcaattcact
     8761 tccaaacact tgaaggaagt tatgataagt aaaaatatgg aagaagatgt tttcgtagct
     8821 gaacaagcaa agagagcctt ggaattacca ttgcattgga aagttcctat gttggaagca
     8881 agatggttca tccatatata cgaaagaaga gaagataaga accacttgtt gttggaattg
     8941 gcaaagatgg aattcaatac attacaagcc atctatcaag aagaattgaa ggaaatctct
     9001 ggttggtgga aggataccgg tttgggtgaa aagttgtcat tcgctagaaa tagattggtc
     9061 gcatctttct tatggtcaat gggtattgcc tttgaacctc aattcgctta ctgtagaaga
     9121 gttttgacaa tctctattgc attgatcacc gttatagatg acatctatga cgtatacggt
     9181 actttggatg aattggaaat ctttacagac gctgttgaaa gatgggatat caactatgca
     9241 ttaaagcatt tgccaggtta catgaagatg tgctttttag ccttgtacaa cttcgtcaac
     9301 gaattcgctt actacgtttt gaagcaacaa gatttcgact tattgttatc tattaaaaac
     9361 gcttggttgg gtttgataca agcctatttg gtagaggcta agtggtacca ttctaagtac
     9421 acacctaagt tggaagaata cttggaaaac ggtttagtct caatcactgg tccattgatc
     9481 atcacaatct cctatttgag tggtactaac cctatcatta aaaaggaatt ggaattcttg
     9541 gaatcaaacc cagatatcgt tcactggtct tcaaaaattt tcagattgca agatgactta
     9601 ggtacttcca gtgacgaaat tcaaagaggt gacgtaccta aatctataca atgctacatg
     9661 catgaaacag gtgcatcaga agaagttgcc agacaacaca ttaaggatat gatgagacaa
     9721 atgtggaaaa aggttaatgc ttacaccgca gataaagact ccccattgac tggtactaca
     9781 accgaattct tgttgaactt agttagaatg tctcatttca tgtatttgca tggtgacggt
     9841 cacggtgtac aaaatcaaga aaccattgat gtcggtttta ctttgttatt ccaacctatt
     9901 ccattagaag ataaacacat ggcattcacc gcatcaccag gtacaaaggg tggtggtggt
     9961 ggtggtggtg gtggtgcttc agaaaaagaa attaggagag agagattctt gaacgttttc
    10021 cctaaattag tagaggaatt gaacgcatcg cttttggctt acggtatgcc taaggaagca
    10081 tgtgactggt atgcccactc attgaactac aacactccag gcggtaagct aaatagaggt
    10141 ttgtccgttg tggacacgta tgctattctc tccaacaaga ccgttgaaca attggggcaa
    10201 gaagaatacg aaaaggttgc cattctaggt tggtgcattg agttgttgca ggcttactgg
    10261 ttggtcgccg atgatatgat ggacaagtcc attaccagaa gaggccaacc atgttggtac
    10321 aaggttcctg aagttgggga aattgccatc tgggacgcat tcatgttaga ggctgctatc
    10381 tacaagcttt tgaaatctca cttcagaaac gaaaaatact acatagatat caccgaattg
    10441 ttccatgagg tcaccttcca aaccgaattg ggccaattga tggacttaat cactgcacct
    10501 gaagacaaag tcgacttgag taagttctcc ctaaagaagc actccttcat agttactttc
    10561 aagactgctt actattcttt ctacttgcct gtcgcattgg ccatgtacgt tgccggtatc
    10621 acggatgaaa aggatttgaa acaagccaga gatgtcttga ttccattggg tgaatacttc
    10681 caaattcaag atgactactt agactgcttc ggtaccccag aacagatcgg taagatcggt
    10741 acagatatcc aagataacaa atgttcttgg gtaatcaaca aggcattaga acttgcttcc
    10801 gcagaacaaa gaaagacttt agacgaaaat tacggtaaga aggactcagt cgcagaagcc
    10861 aaatgcaaaa agattttcaa tgacttgaaa attgaacagc tataccacga atatgaagag
    10921 tctattgcca aggatttgaa ggccaaaatt tctcaggtcg atgagtctcg tggcttcaaa
    10981 gctgatgtct taactgcgtt cttgaacaag gtttacaaga gaagtaaata gtaaccatgg
    11041 gcggattgag agcaaatcgt taagttcagg tcaagtaaaa attgatttcg aaaactaatt
    11101 tctcttatac aatcctttga ttggaccgtc atcctttcga atataagatt ttgttaagaa
    11161 tattttagac agagatctac tttatattta atatctagat attacataat ttcctctcta
    11221 ataaaatatc attaataaaa taaaaatgaa gcgatttgat tttgtgttgt caacttagtt
    11281 tgccgctatg cctcttgggt aatgctatta ttgaatcgaa gggctttatt atattaccct
    11341 ttagcttatt ctgaggtttc tgtggcgtcc cgggaatctc g
//
