LOCUS       sequence-371285-        3839 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Knockdown of F40F8.11 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3839)
  TITLE     Seung-Jae Lee RNAi Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: F40F8.11 RNAi
FEATURES             Location/Qualifiers
     source          1..3839
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(368..1159,1160..1228))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     991..1010
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1229..1333)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1359..1814)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1496..1517)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1708..1727
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1941..1963
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1955..1972
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1956..1972
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1982..2000
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3161..3177)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3161..3177)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(3235..3253)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3277..3294)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(3448..3839,1..197))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3528..3547)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
       61 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      121 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      181 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
      241 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
      301 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
      361 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
      421 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
      481 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
      541 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
      601 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
      661 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
      721 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
      781 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
      841 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
      901 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
      961 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1021 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1081 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1141 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1201 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     1261 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     1321 taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt
     1381 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     1441 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     1501 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     1561 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     1621 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     1681 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     1741 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     1801 gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     1861 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     1921 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc
     1981 gtaatacgac tcactatagg gcgaattggg tactcagaaa gtgccgatgc tcttcgtgtc
     2041 ggagatgaat ccaaatttct gaaaataata attattaatg agccattaaa ataattgcct
     2101 aaccgatgac gtgtccatag gctctgctgg ggcgttgttg actctgagag cattgaaaat
     2161 cctggacata tcgacaacct ggatttctcc cgagctgatc ttctgtcgta actgcataag
     2221 aatcactccg agaacgttgc gtccttgtgc aaaaactggg cagtgttgaa ctgttgtctt
     2281 gttaagtggg aaagtagcag gaattttcaa atgtttcaca ccagctttcg ataaatcatc
     2341 caaccatttt ttaatcgccg ggatcgtaca tcctgttccg tatatactgt cacgagcgaa
     2401 cgcatggacc agaatgttat ctccgctttc tttgagagct tcgcgcaaat gagcagactg
     2461 ggtgactttc gcatgaagag ctttttggat cgtctcgaga cctttgctgt tcctccattc
     2521 atccaccttc tccttgctga tattcgctgc tttgattata tccttagcta gtgcgctgtg
     2581 cgactttttc tccgcatctt cgatagtttc aacgataggt tttccgttct catcacgaac
     2641 acctctgttc aatttgtcgc ttgatactcc agtcaaatca gtgactttgc aaatttggta
     2701 gtagtggtcg cagcttccat agatcttatt gtcgatgaga actgggaact gaaacaaaaa
     2761 atttaaaagt aattaattaa tcttttaaaa aatgtgtccc tacattgtgt ttagtggtga
     2821 aaacactgtc gaatccgtgg aaagctgtga tattctcgga agaaacattc agagtgatgg
     2881 caggagaatc atcaggaaga tgaacatatg gaatatcatc cttattgttt gatggtttcg
     2941 gagcgccatt gaatcgtcct cctcgattga aacctccacg acctcttcct ccgcttcctc
     3001 tcccgcgacc acgtccacct cctcctccac gagcagtttg attttgaagt ccgttggtct
     3061 cccaactgtt accttccaat cggtggtttg gcatagcttg atatcgaatt cctgcagccc
     3121 gggggatcca cgcgtcacgt ggctagccat ggaaccggtg gatccactag ttctagagcg
     3181 gccgccaccg cggtggagct cgaattcatc gatgatatca gatctgccgg tctccctata
     3241 gtgagtcgta ttaatttcga taagccaggt tgcttcctcg ctcactgact cgctgcgctc
     3301 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3361 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3421 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3481 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3541 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3601 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3661 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3721 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3781 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcg
//
