LOCUS       sequence-371338-       10359 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of full length human integrin beta 3 with N305T.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Lin FY, Li J, Xie Y, Zhu J, Huong Nguyen TT, Zhang Y, Zhu J, 
            Springer TA
  TITLE     A general chemical principle for creating closure-stabilizing 
            integrin inhibitors.
  JOURNAL   Cell. 2022 Sep 15;185(19):3533-3550.e27. doi: 
            10.1016/j.cell.2022.08.008.
  PUBMED    36113427
REFERENCE   2  (bases 1 to 10359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.08"; journalName: "Cell"; date: "2022-09-15- 
            15"; volume: "185"; issue: "19"; pages: "3533-3550.e27"
COMMENT     Sequence Label: pD2529-CAG-b3 N305T
FEATURES             Location/Qualifiers
     source          1..10359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(342..1151)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1065..1084
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(1170..1217)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             complement(1643..2242)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1726..1746)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2223..2242
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(2252..2751)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2300..2319)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     2693..2716
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     enhancer        2835..3138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3416..4432
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4356..4378
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4440..4459
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             6901..6957
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6958..7668
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7103..7121)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7371..7390
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    9262..9317
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9316..9335)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      complement(join(10027..10359,1..256))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10107..10126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
       61 tgctacagag ttcttgaagt ggtgggctaa ctacggctac actagaagaa cagtatttgg
      121 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      181 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      241 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      301 cgacgcgcgc gtaactcacg ttaagggatt ttggtcatga gttagaaaaa ctcatcgagc
      361 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
      421 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
      481 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
      541 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
      601 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
      661 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gaggcgaaat
      721 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg agtgcaaccg gcgcaggaac
      781 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaac
      841 gctgtttttc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
      901 tgcttgatgg tcggaagtgg cataaattcc gtcagccagt ttagtctgac catctcatct
      961 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     1021 ttcccataca agcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     1081 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctcgacgt ttcccgttgg
     1141 atatggctca ttttttactt cctcaccttg tcgtattata ctatgccgat atactatgcc
     1201 gatgattaat tgtcgacact gcgggggctc tgtgtggtaa gcaggtctta acctttttac
     1261 tgccaatgac gcatgggata cgtcgtggca gtaaaagggc ttaaatgcca acgacgcgtc
     1321 ccatacgttg ttggcatttt aattcttctc tctgcagcgg cagcatgtgc cgccgctgca
     1381 gagagtttct agcgatgaca gcccctctgg gcaacgagcc gggggggctg tctttcttta
     1441 tgttttaaat gcactgacct cccacattcc ctttttagta aaatattcag aaataattta
     1501 aatacatcat tgcaatgaaa ataaatgttt tttattaggc agaatccaga tgctcaaggc
     1561 ccttcataat atcccccagt ttagtagttg gacttaggga acaaaggaac ctttaataga
     1621 aattggacag caagaaagcg agtcaggcac cgggcttgcg ggtcatgcac caggtgcgcg
     1681 gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga
     1741 ggttgcgggg cgcggatgtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca
     1801 ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gacacgccga
     1861 cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct
     1921 gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga
     1981 acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt
     2041 ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct
     2101 cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg
     2161 cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca
     2221 ccgtgggctt gtactcggtc atggtggcgg acgaaaggcc cggagatgag gaagaggaga
     2281 acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg gaggaccttc
     2341 gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca ccccttccca
     2401 gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc caaaggccta
     2461 cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag actagtgagt cgtgctactt
     2521 ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc ctgactaggg
     2581 gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg cgcctaccgg
     2641 tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg cccagcgggg
     2701 ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc cggtagagaa
     2761 acttgatctg tcgccgcaat tcaaacttcg tgaggctccg gtgcccgtca gtgacctgct
     2821 atactctgga gacgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2881 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2941 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3001 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3061 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3121 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
     3181 cccccctccc cacccccaat tttgtattta tttatttttt aattattttg tgcagcgatg
     3241 ggggcggggg gggggggggc gcgcgccrgg cggggsgggg sggggsgrgg ggsggggsgg
     3301 ggsgrggcgg rgaggkgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt
     3361 tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt
     3421 cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc gcctcgcgcc gcccgccccg
     3481 gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg
     3541 ctgtaattag cgcttggttt aatgacggct tgtttctttt ctgtggctgc gtgaaagcct
     3601 tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc tcggggggtg cgtgcgtgtg
     3661 tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc ggcggctgtg agcgctgcgg
     3721 gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag gggagcgcgg ccgggggcgg
     3781 tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt
     3841 gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg caaccccccc tgcacccccc
     3901 tccccgagtt gctgagcacg gcccggcttc gggtgcgggg ctccgtacgg ggcgtggcgc
     3961 ggggctcgcc gtgccgggcg gggggtggcg gcaggkgggg gtgccgggcg gggcggggcc
     4021 gcctcgggcc ggggrgggct cgggggaggg gcgcggcggc ccccggagcg ccggcggctg
     4081 tcgaggcgcg gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg
     4141 acttcctttg tcccaaatct gtgcggagcc gaaatctggg aggcgccgcc gcaccccctc
     4201 tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct
     4261 tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag cctcggggct gtccgcgggg
     4321 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     4381 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     4441 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattcggct tgatcgaagc
     4501 cgtctcaggg gagagaccac acccaagctg tctagagccg ccaccatgcc tttgctcctc
     4561 ctgttgccct tgctctgggc tggcgccctc gcggggccca acatctgtac cacgcgaggt
     4621 gtgagctcct gccagcagtg cctggctgtg agccccatgt gtgcctggtg ctctgatgag
     4681 gccctgcctc tgggctcacc tcgctgtgac ctgaaggaga atctgctgaa ggataactgt
     4741 gccccagaat ccatcgagtt cccagtgagt gaggcccgag tactagagga caggcccctc
     4801 agcgacaagg gctctggaga cagctcccag gtcactcaag tcagtcccca gaggattgca
     4861 ctccggctcc ggccagatga ttcgaagaat ttctccatcc aagtgcggca ggtggaggat
     4921 taccctgtgg acatctacta cttgatggac ctgtcttact ccatgaagga tgatctgtgg
     4981 agcatccaga acctgggtac caagctggcc acccagatgc gaaagctcac cagtaacctg
     5041 cggattggct tcggggcatt tgtggacaag cctgtgtcac catacatgta tatctcccca
     5101 ccagaggccc tcgaaaaccc ctgctatgat atgaagacca cctgcttgcc catgtttggc
     5161 tacaaacacg tgctgacgct aactgaccag gtgacccgct tcaatgagga agtgaagaag
     5221 cagagtgtgt cacggaaccg agatgcccca gagggtggct ttgatgccat catgcaggct
     5281 acagtctgtg atgaaaagat tggctggagg aatgatgcat cccacttgct ggtgtttacc
     5341 actgatgcca agactcatat agcattggac ggaaggctgg caggcattgt ccagcctaat
     5401 gacgggcagt gtcatgttgg tagtgacaat cattactctg cctccactac catggattat
     5461 ccctctttgg ggctgatgac tgagaagcta tcccagaaaa acatcacctt gatctttgca
     5521 gtgactgaaa atgtagtcaa tctctatcag aactatagtg agctcatccc agggaccaca
     5581 gttggggttc tgtccatgga ttccagcaat gtcctccagc tcattgttga tgcttatggg
     5641 aaaatccgtt ctaaagtaga gctggaagtg cgtgacctcc ctgaagagtt gtctctatcc
     5701 ttcaatgcca cctgcctcaa caatgaggtc atccctggcc tcaagtcttg tatgggactc
     5761 aagattggag acacggtgag cttcagcatt gaggccaagg tgcgaggctg tccccaggag
     5821 aaggagaagt cctttaccat aaagcccgtg ggcttcaagg acagcctgat cgtccaggtc
     5881 acctttgatt gtgactgtgc ctgccaggcc caagctgaac ctaatagcca tcgctgcaac
     5941 aatggcaatg ggacctttga gtgtggggta tgccgttgtg ggcctggctg gctgggatcc
     6001 cagtgtgagt gctcagagga ggactatcgc ccttcccagc aggacgaatg cagcccccgg
     6061 gagggtcagc ccgtctgcag ccagcggggc gagtgcctct gtggtcaatg tgtctgccac
     6121 agcagtgact ttggcaagat cacgggcaag tactgcgagt gtgacgactt ctcctgtgtc
     6181 cgctacaagg gggagatgtg ctcaggccat ggccagtgca gctgtgggga ctgcctgtgt
     6241 gactccgact ggaccggcta ctactgcaac tgtaccacgc gtactgacac ctgcatgtcc
     6301 agcaatgggc tgctgtgcag cggccgcggc aagtgtgaat gtggcagctg tgtctgtatc
     6361 cagccgggct cctatgggga cacctgtgag aagtgcccca cctgcccaga tgcctgcacc
     6421 tttaagaaag aatgtgtgga gtgtaagaag tttgaccggg gagccctaca tgacgaaaat
     6481 acctgcaacc gttactgccg tgacgagatt gagtcagtga aagagcttaa ggacactggc
     6541 aaggatgcag tgaattgtac ctataagaat gaggatgact gtgtcgtcag attccagtac
     6601 tatgaagatt ctagtggaaa gtccatcctg tatgtggtag aagagccaga gtgtcccaag
     6661 ggccctgaca tcctggtggt cctgctctca gtgatggggg ccattctgct cattggcctt
     6721 gccgccctgc tcatctggaa actcctcatc accatccacg accgaaaaga atttgctaaa
     6781 tttgaggaag aacgcgccag agcaaaatgg gacacagcca acaacccact gtataaagag
     6841 gccacgtcta ccttcaccaa tatcacgtac cggggcactg gtaccagtac tgggagtggg
     6901 gccaccaact tttctctgct caagcaagcc ggggatgttg aagaaaatcc agggccaatg
     6961 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     7021 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     7081 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     7141 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     7201 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     7261 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     7321 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     7381 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     7441 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     7501 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     7561 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     7621 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtgata aagtacttga
     7681 taagctcttc aggttgaggt ctctaaaagc gtcttcctgt tctcatcaca tcatatcaag
     7741 gttatatacc atcaatattg ccacagatgt tacttagcct tttaatattt ctctaattta
     7801 gtgtatatgc aatgatagtt ctctgatttc tgagattgag tttctcatgt gtaatgatta
     7861 tttagagttt ctctttcatc tgttcaaatt tttgtctagt tttatttttt actgatttgt
     7921 aagacttctt tttataatct gcatattaca attctcttta ctggggtgtt gcaaatattt
     7981 tctgtcattc tatggcctga cttttcttaa tggtttttta attttaaaaa taagtcttaa
     8041 tattcatgca atctaattaa caatcttttc tttgtggtta ggactttgag tcataagaaa
     8101 tttttctcta cactgaagtc atgatggcat gcttctatat tattttctaa aagatttaaa
     8161 gttttgcctt ctccatttag acttataatt cactggaatt tttttgtgtg tatggtatga
     8221 catatgggtt cccttttatt ttttacatat aaatatattt ccctgttttt ctaaaaaaga
     8281 aaaagatcat cattttccca ttgtaaaatg ccatattttt ttcataggtc acttacatat
     8341 atcaatgggt ctgtttctga gctctactct attttatcag cctcactgtc tatccccaca
     8401 catctcatgc tttgctctaa atcttgatat ttagtggaac attctttccc attttgttct
     8461 acaagaatat ttttgttatt gtctttgggc tttctatata cattttgaaa tgaggttgac
     8521 aagttaataa caggcctatt gattggaaag tttgtcaacg aattgtgggt cttttggggt
     8581 ttgctgcccc ttttacgcaa tgtggatatc ctgctttaat gcctttatat gcatgtatac
     8641 aagcaaaaca ggcttttact ttctcgccaa cttacaaggc ctttctcagt aaacagtata
     8701 tgacccttta ccccgttgct cggcaacggc ctggtctgtg ccaagtgttt gctgacgcaa
     8761 cccccactgg ttggggcttg gccataggcc atcagcgcat gcgtggaacc tttgtgtctc
     8821 ctctgccgat ccatactgcg gaactcctag ccgcttgttt tgctcgcagc aggtctggag
     8881 caaacctcat cgggaccgac aattctgtcg tactctcccg caagtataca tcgtttccat
     8941 ggctgctagg ctgtgctgcc aactggatcc tgcgcgggac gtcctttgtt tacgtcccgt
     9001 cggcgctgaa tcccgcggac gacccctccc ggggccgctt ggggctctac cgcccgcttc
     9061 tccgtctgcc gtaccgtccg accacggggc gcacctctct ttacgcggac tccccgtctg
     9121 tgccttctca tctgccggac cgtgtgcact tcgcttcacc tctgcacgtc gcatggaggc
     9181 caccgtgaac gcccaccgga acctgcccaa ggtcttgcat aagaggactc ttggactttc
     9241 agcaatgtca tctcagtggc taataaagga aatttatttt cattgcaata gtgtgttgga
     9301 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     9361 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     9421 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     9481 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     9541 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     9601 gactactccc agtcatagct gtccctcttc tcttatggag atctttgcat ttttagacat
     9661 ttagaagcct atatcttgtt acagaattgg aattacacaa aaattctacc atattttgaa
     9721 agcttaggtt gttctgaaaa aaacaatata ttgttttcct gggtaaacta aaagtcccct
     9781 cgaggaaagg cccctaaagt gaaacagtgc aaaacgttca aaaactgtct ggcaatacaa
     9841 gttccacttt gaccaaaacg gctggcagta aaagggttaa gaagactgtc agccttgagc
     9901 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     9961 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    10021 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    10081 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    10141 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    10201 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    10261 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    10321 cggtaactat cgtcttgagt ccaacccggt aagacacga
//
