LOCUS       sequence-372023-       10175 bp    DNA     circular SYN 15-APR-2025
DEFINITION  EGFP fused to the C-terminus of NUDT1 and a hygromycin resistance 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10175)
  TITLE     Mechanisms of oxidative damage repair
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Hygro-EF1A-hNUDT1/EGFP
FEATURES             Location/Qualifiers
     source          1..10175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..244
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             245..425
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    472..597
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1090..1323
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1508..1552
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1818..1935
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1953..1973
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1974..3152
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2205..3143
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3100..3120
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3152..3176
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3720..3722,3723..4436)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3720..3722,3723..4433)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3762..3783)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4023..4042)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4370..4391
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4437..4461)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4479..5067
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4532..4552)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4950..4961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5098..5597
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5133..5156)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5530..5549
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5614..6639
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5614..6639
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6711..6944
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7016..7137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7053..7072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7107..7126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7177..7312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7332..7351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7333..7351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7361..7378)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7361..7377)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7370..7392)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7519..7974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7606..7625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7816..7837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8000..8104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8105..8173,8174..8965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8323..8342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9136..9724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9625..9644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9878..9895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10012..10033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10048..10053,10054..10071,10072..10078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10086..10102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10091..10113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10110..10126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10110..10126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10145..10165
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10147..10165
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
       61 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
      121 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
      181 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
      241 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      301 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      361 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      421 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
      481 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
      541 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
      601 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
      661 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
      721 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
      781 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
      841 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
      901 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
      961 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     1021 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     1081 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     1141 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     1201 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     1261 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     1321 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     1381 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     1441 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     1501 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     1561 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     1621 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     1681 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     1741 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     1801 tcgacggtat cgctagcttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     1861 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     1921 aaaattcaaa attttactag tgattatcgg atcaactttg tatagaaaag ttgggctccg
     1981 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     2041 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     2101 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     2161 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     2221 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     2281 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     2341 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     2401 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     2461 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     2521 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     2581 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     2641 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggtctcg
     2701 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     2761 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     2821 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     2881 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     2941 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     3001 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3061 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3121 aaagtttttt tcttccattt caggtgtcgt gacaagtttg tacaaaaaag caggctgcca
     3181 ccatgagtgg aattagccct cagcagatgg gggagccaga aggcagttgg agtgggaaga
     3241 acccagggac catgggcgcc tccaggctct ataccctggt gctggtcctg cagcctcagc
     3301 gagttctcct gggcatgaaa aagcgaggct tcggggccgg ccggtggaat ggctttgggg
     3361 gcaaagtgca agaaggagag accatcgagg atggggctag gagggagctg caggaggaga
     3421 gcggtctgac agtggacgcc ctgcacaagg tgggccagat cgtgtttgag ttcgtgggcg
     3481 agcctgagct catggacgtg catgtcttct gcacagacag catccagggg acccccgtgg
     3541 agagcgacga aatgcgccca tgctggttcc agctggatca gatccccttc aaggacatgt
     3601 ggcccgacga cagctactgg tttccactcc tgcttcagaa gaagaaattc cacgggtact
     3661 tcaagttcca gggtcaggac accatcctgg actacacact ccgcgaggtg gacacggtcg
     3721 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3781 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3841 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3901 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3961 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4021 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4081 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4141 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4201 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4261 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4321 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4381 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtagaccc
     4441 agctttcttg tacaaagtgg tgataatcga attccgataa tcaacctctg gattacaaaa
     4501 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     4561 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4621 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     4681 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4741 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4801 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4861 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4921 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4981 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     5041 ggatctccct ttgggccgcc tccccgcatc gggaattccc gcggttcgaa ttctaccggg
     5101 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     5161 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     5221 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     5281 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     5341 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     5401 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     5461 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     5521 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     5581 catctccggg cctttcgacc tcacgtggcc accatgaaaa agcctgaact caccgcgacg
     5641 tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg
     5701 gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg
     5761 gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg
     5821 gccgcgctcc cgattccgga agtgcttgac attggggaat ttagcgagag cctgacctat
     5881 tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc
     5941 gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag
     6001 acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat
     6061 ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc
     6121 gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc
     6181 gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc
     6241 cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc
     6301 gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc
     6361 gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt
     6421 ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg
     6481 cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc
     6541 gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga
     6601 aaccgacgcc ccagcactcg tccgagggca aaggaatagg gtacctttaa gaccaatgac
     6661 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6721 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6781 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6841 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6901 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6961 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7021 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7081 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7141 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7201 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7261 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     7321 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7381 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7441 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7501 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7561 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7621 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7681 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7741 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7801 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7861 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7921 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8281 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8341 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9241 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9541 cacgcttccc gaagagagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9841 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9901 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9961 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    10021 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    10081 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    10141 gcgcgcaatt aaccctcact aaagggaaca aaagc
//
