LOCUS       sequence-372189-       11052 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Split luciferase construct with nLuc fused to C-terminus of WWE 
            domain, linked by T2A to cLuc fused to C-terminus of WWE domain, 
            linked by IRES to puromycin resistance cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Koczor CA, Haider AJ, Saville KM, Li J, Andrews JF, Beiser AV, Sobol
            RW
  TITLE     Live Cell Detection of Poly(ADP-Ribose) for Use in Genetic and 
            Genotoxic Compound Screens.
  JOURNAL   Cancers (Basel). 2022 Jul 28;14(15). pii: cancers14153676. doi: 
            10.3390/cancers14153676.
  PUBMED    35954352
REFERENCE   2  (bases 1 to 11052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2022-07-28"; volume: "14(15). pii: 
            cancers14153676. doi"; pages: " 10.3390/cancers14153676"
COMMENT     Sequence Label: 
            pLV-EF1A-WWE-HA-nLuc-FLAG-T2A-WWE-myc-cLuc-FLAG-IRES-Puro
FEATURES             Location/Qualifiers
     source          1..11052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1929..1949
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1950..3128
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2181..3119
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3076..3096
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3128..3152
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3153..3162
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3468..3494
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4743..4766
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4776..4829
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5133..5162
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5625..5648
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(5652..5676)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(5677..6251,6252..6254,6255..6263)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5844..5861)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6071..6090
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6264..6863
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6264..6283)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6760..6780
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6864..6884)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6897..7485
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6950..6970)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7368..7379)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7573..7806
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7878..7999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7915..7934)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7969..7988
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8039..8174
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8194..8213)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8195..8213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8223..8240)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8223..8239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8232..8254)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8381..8836
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8468..8487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8678..8699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8862..8966
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8967..9035,9036..9827)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9185..9204)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9998..10586
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10487..10506
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10740..10757
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10874..10895
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10910..10915,10916..10933,10934..10940)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10948..10964
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10953..10975
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10972..10988
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10972..10988
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11007..11027
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11009..11027
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtatca actttgtata gaaaagttgg gctccggtgc ccgtcagtgg gcagagcgca
     1981 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2041 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2101 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2161 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2221 cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg
     2281 atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc
     2341 cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct
     2401 ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt
     2461 gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc
     2521 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     2581 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     2641 ctcaagctgg ccggcctgct ctggtgcctg gtctcgcgcc gccgtgtatc gccccgccct
     2701 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     2761 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     2821 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     2881 agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt
     2941 taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg
     3001 aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg
     3061 gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg
     3121 tgtcgtgaca agtttgtaca aaaaagcagg ctgccaccat gggaaatggt gaatatgcat
     3181 ggtattatga aggaagaaat gggtggtggc agtacgatga gcgcactagt agagagctgg
     3241 aagatgcttt ttccaaaggt aaaaagaaca ctgaaatgtt aattgctggc tttctgtatg
     3301 tcgctgatct tgaaaacatg gttcaatata ggagaaatga acatggacgt cgcaggaaga
     3361 ttaagcgaga tataatagat ataccaaaga agggagtagc tggacttagg atctccggac
     3421 agatcagcta cgccagccgg ggcggaggta gctctggcgg aggtgcctac ccatacgatg
     3481 ttccagatta cgctgcagaa gatgccaaaa acattaagaa gggcccagcg ccattctacc
     3541 cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg
     3601 tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc tacgccgagt
     3661 acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg aatacaaacc
     3721 atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg ttgggtgccc
     3781 tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc gagctgctga
     3841 acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga
     3901 tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg gatagcaaga
     3961 ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg ccacccggct
     4021 tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc gccctgatca
     4081 tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac cgcaccgctt
     4141 gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc cccgacaccg
     4201 ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg ctgggctact
     4261 tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta ttcttgcgca
     4321 gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt agcttcttcg
     4381 ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc gccagcggcg
     4441 gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac ctaccaggca
     4501 tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc cccgaagggg
     4561 acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag gtggtggact
     4621 tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca
     4681 tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg
     4741 gcgactacaa ggacgacgat gacaagggaa gcggagaggg caggggaagt cttctaacat
     4801 gcggggacgt ggaggaaaat cccggcccca tgggcaacgg cgagtatgca tggtattacg
     4861 aaggcagaaa cggatggtgg caatatgatg aaagaacttc tcgagaactg gaagatgcgt
     4921 tctctaaagg gaagaagaat actgaaatgt taatagcggg atttctttac gtcgccgact
     4981 tagaaaacat ggtgcaatac cggcgaaatg agcacggccg gagaaggaaa ataaagaggg
     5041 atattataga tatccccaag aaaggtgtgg ctgggttaag gtcccagatc agctacgcca
     5101 gccggggcgg aggtagctct ggcggaggtg ccgagcagaa gctgatcagc gaggaagacc
     5161 tgtccggcag cggctacgtt aacaaccccg aggctacaaa cgctctcatc gacaaggacg
     5221 gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc ttcatcgtgg
     5281 accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc gaactggaga
     5341 gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg cccgacgacg
     5401 atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga
     5461 aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg cgcggtggtg
     5521 ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc
     5581 gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtggactac aaggacgacg
     5641 atgacaagtg aacccagctt tcttgtacaa agtgggcccc tctccctccc ccccccctaa
     5701 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc
     5761 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac
     5821 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt
     5881 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg
     5941 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata
     6001 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga
     6061 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt
     6121 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
     6181 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     6241 acgatgataa tatggccaca accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6301 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6361 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6421 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6481 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6541 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6601 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6661 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6721 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6781 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6841 tgacccgcaa gcccggtgcc tgacaacttt attatacata gttgatcaat tccgataatc
     6901 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6961 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7021 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7081 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7141 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7201 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7261 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     7321 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7381 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7441 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg gaattcccgc
     7501 ggttcgcttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7561 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7621 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7681 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7741 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7801 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7861 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7921 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7981 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     8041 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     8101 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     8161 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     8221 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     8281 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     8341 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     8401 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8461 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8521 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8581 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8641 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8701 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8761 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8821 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8881 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8941 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     9001 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     9061 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     9121 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     9181 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     9241 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     9301 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9361 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9421 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9481 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9541 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9601 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9661 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9721 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9781 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9841 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9901 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9961 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    10021 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    10081 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    10141 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10201 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10261 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10321 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10381 agcgtgagct atgagaaagc gccacgcttc ccgaagagag aaaggcggac aggtatccgg
    10441 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10501 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10561 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10621 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10681 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10741 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10801 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10861 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    10921 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    10981 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
    11041 agctgcaagc tt
//
