LOCUS       sequence-372850-       12094 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Express Icelandic mutant APP from Ubiquitin C promoter, 
            co-expressing mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12094)
  TITLE     Hart Lab APP and APOE Plasmids 2022
REFERENCE   2  (bases 1 to 12094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUGW-APPIce-T2A-mCherry
FEATURES             Location/Qualifiers
     source          1..12094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     639..658
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             complement(1160..1171)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     CDS             1651..1671
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     CDS             2918..2971
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2984..3004
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3005..3715
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGREFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3150..3168)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3418..3437
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     3612..3635
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    3755..4343
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3808..3828)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4226..4237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4346..4362)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4347..4363)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4868..5048
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5074..5091)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5080..5304
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5350..5778
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5437..5456)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5647..5668
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5787..5807)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5792..6121
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5972..6107
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6169..6216
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6235..6609
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6739..6860
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6776..6795)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6830..6849
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6909..6925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6909..6925)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6922..6944)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6933..6949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6957..6963,6964..6981,6982..6987))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7002..7023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7140..7157)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7311..7899)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7391..7410)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8070..8861,8862..8930))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8693..8712
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8931..9035)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9110..9129)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9301..9680
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9682..9880
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9835..9855
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9898..10078
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10125..10250
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10743..10976
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11161..11205
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11503..11620
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        join(11685..12094,1..802)
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
ORIGIN
        1 ctgctgggct ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg
       61 gagagaccgc caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt
      121 ggggggagcg cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg
      181 cgggctgtga ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc
      241 ttgaggcctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
      301 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
      361 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
      421 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
      481 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
      541 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
      601 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
      661 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
      721 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
      781 gtttttggct tttttgttag acgaagcttg ggctgcaggt cgactggcca ccatgctgcc
      841 cggtttggca ctgctcctgc tggccgcctg gacggctcgg gcgctggagg tacccactga
      901 tggtaatgct ggcctgctgg ctgaacccca gattgccatg ttctgtggca gactgaacat
      961 gcacatgaat gtccagaatg ggaagtggga ttcagatcca tcagggacca aaacctgcat
     1021 tgataccaag gaaggcatcc tgcagtattg ccaagaagtc taccctgaac tgcagatcac
     1081 caatgtggta gaagccaacc aaccagtgac catccagaac tggtgcaagc ggggccgcaa
     1141 gcagtgcaag acccatcccc actttgtgat tccctaccgc tgcttagttg gtgagtttgt
     1201 aagtgatgcc cttctcgttc ctgacaagtg caaattctta caccaggaga ggatggatgt
     1261 ttgcgaaact catcttcact ggcacaccgt cgccaaagag acatgcagtg agaagagtac
     1321 caacttgcat gactacggca tgttgctgcc ctgcggaatt gacaagttcc gaggggtaga
     1381 gtttgtgtgt tgcccactgg ctgaagaaag tgacaatgtg gattctgctg atgcggagga
     1441 ggatgactcg gatgtctggt ggggcggagc agacacagac tatgcagatg ggagtgaaga
     1501 caaagtagta gaagtagcag aggaggaaga agtggctgag gtggaagaag aagaagccga
     1561 tgatgacgag gacgatgagg atggtgatga ggtagaggaa gaggctgagg aaccctacga
     1621 agaagccaca gagagaacca ccagcattgc caccaccacc accaccacca cagagtctgt
     1681 ggaagaggtg gttcgagttc ctacaacagc agccagtacc cctgatgccg ttgacaagta
     1741 tctcgagaca cctggggatg agaatgaaca tgcccatttc cagaaagcca aagagaggct
     1801 tgaggccaag caccgagaga gaatgtccca ggtcatgaga gaatgggaag aggcagaacg
     1861 tcaagcaaag aacttgccta aagctgataa gaaggcagtt atccagcatt tccaggagaa
     1921 agtggaatct ttggaacagg aagcagccaa cgagagacag cagctggtgg agacacacat
     1981 ggccagagtg gaagccatgc tcaatgaccg ccgccgcctg gccctggaga actacatcac
     2041 cgctctgcag gctgttcctc ctcggcctcg tcacgtgttc aatatgctaa agaagtatgt
     2101 ccgcgcagaa cagaaggaca gacagcacac cctaaagcat ttcgagcatg tgcgcatggt
     2161 ggatcccaag aaagccgctc agatccggtc ccaggttatg acacacctcc gtgtgattta
     2221 tgagcgcatg aatcagtctc tctccctgct ctacaacgtg cctgcagtgg ccgaggagat
     2281 tcaggatgaa gttgatgagc tgcttcagaa agagcaaaac tattcagatg acgtcttggc
     2341 caacatgatt agtgaaccaa ggatcagtta cggaaacgat gctctcatgc catctttgac
     2401 cgaaacgaaa accaccgtgg agctccttcc cgtgaatgga gagttcagcc tggacgatct
     2461 ccagccgtgg cattcttttg gggctgactc tgtgccagcc aacacagaaa acgaagttga
     2521 gcctgttgat gcccgccctg ctgccgaccg aggactgacc actcgaccag gttctgggtt
     2581 gacaaatatc aagacggagg agatctctga agtgaagatg gatacagaat tccgacatga
     2641 ctcaggatat gaagttcatc atcaaaaatt ggtgttcttt gcagaagatg tgggttcaaa
     2701 caaaggtgca atcattggac tcatggtggg cggtgttgtc atagcgacag tgatcgtcat
     2761 caccttggtg atgctgaaga agaaacagta cacatccatt catcatggtg tggtggaggt
     2821 tgacgccgct gtcaccccag aggagcgcca cctgtccaag atgcagcaga acggctacga
     2881 aaatccaacc tacaagttct ttgagcagat gcagaacgag ggcaggggaa gtcttctaac
     2941 atgcggggac gtggaggaaa atcccggccc tctagagatg gctccaaaga agaagcgtaa
     3001 ggtaatggtg agcaagggcg aggaggacaa catggccatc atcaaggagt tcatgcgctt
     3061 caaggtgcac atggagggct ccgtgaacgg ccgcgagttc gagatcgagg gcgagggcga
     3121 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     3181 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3241 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3301 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     3361 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     3421 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     3481 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     3541 cgccgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     3601 cgtcaacatc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     3661 cgagcgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaattaa
     3721 gctagtctag aattccgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     3781 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3841 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3901 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3961 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     4021 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     4081 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     4141 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     4201 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     4261 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     4321 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     4381 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     4441 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     4501 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     4561 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     4621 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     4681 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     4741 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     4801 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     4861 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4921 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4981 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     5041 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     5101 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5161 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5221 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5281 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     5341 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5401 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5461 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5521 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5581 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5641 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5701 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5761 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5821 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5881 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5941 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6001 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6061 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6121 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     6181 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     6241 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     6301 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     6361 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     6421 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     6481 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     6541 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     6601 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6661 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6721 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6781 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6841 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6901 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6961 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7021 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7081 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7141 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7201 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7261 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7321 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7381 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7441 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7501 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7561 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7621 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7681 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7741 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7801 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7861 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7921 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7981 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8041 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8101 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8161 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8221 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8281 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8341 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8401 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8461 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8521 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8581 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8641 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8701 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8761 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8821 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8881 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8941 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9001 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     9061 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     9121 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     9181 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     9241 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     9301 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     9361 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     9421 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     9481 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     9541 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     9601 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     9661 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     9721 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     9781 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     9841 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     9901 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     9961 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
    10021 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
    10081 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
    10141 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
    10201 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
    10261 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
    10321 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
    10381 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
    10441 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
    10501 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
    10561 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
    10621 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    10681 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    10741 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    10801 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    10861 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    10921 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    10981 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    11041 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    11101 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    11161 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    11221 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    11281 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    11341 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    11401 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
    11461 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
    11521 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    11581 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
    11641 cagagatcca gtttggttaa ttaacccgtg tcggctccag atctggcctc cgcgccgggt
    11701 tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg
    11761 gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata
    11821 agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga
    11881 ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct
    11941 cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc
    12001 gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt
    12061 ggatcgctgt gatcgtcact tggtgagtag cggg
//
