LOCUS       sequence-372920-        4487 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 4487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_75
FEATURES             Location/Qualifiers
     source          1..4487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(13..19,20..37,38..43))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    58..79
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(196..213)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(367..955)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(447..466)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1126..1917,1918..1986))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1749..1768
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1987..2091)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2159..2177
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2255..2274)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2374..2396
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3363..3383)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     4131..4159
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(4452..4468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4452..4468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4465..4487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    join(4476..4487,1..5)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
       61 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
      121 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      181 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      241 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      301 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      361 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      421 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      481 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      541 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      601 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      661 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      721 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      781 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      841 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      901 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      961 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1021 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1081 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1141 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1201 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1261 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1321 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1381 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1441 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1501 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1561 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1621 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1681 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1741 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1801 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1861 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1921 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1981 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2041 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2101 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     2161 taggcgtatc acgaggccct taaggccgcc tgatgcggta ttttctcctt acgcatctgt
     2221 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     2281 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     2341 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     2401 caccgtcatc accgaaacgc gcgagacgaa agggcccgta cggccgacta gtaggaaaca
     2461 gttatgtttg gtatattggg aatgtattct gtcatttaag gcaaaaaccc catagacact
     2521 actccacttt caaattatca acttctactc accttccact gagcctcaaa cccaaacctt
     2581 cttccgatct ttcttgcatc gtgctcatca atacttgtga ttttagcaag aaaaacgccg
     2641 tcctcgaaat cttgacaaac aataggggaa accccaaaaa gcaaaagcag gaaaattaat
     2701 ttcataaact ttcacttgat tgaaaaattc caaaaatact ccaaaacttt ttcacgatga
     2761 gcatgatttg acgtcatgag aggcccagac gtgcgaagaa ataaaaattg cggtcataaa
     2821 ctgaaacgta acatatgata aggtattttg tgtgcggttt tttctatgat gtttcaaaaa
     2881 aatttatcag aagtaaaaaa cataagaagt gtacacagaa cattgtgtag attttgggat
     2941 agaattatta ttaatggggg gagggagcac aatttttttc gactttttat tacgaattaa
     3001 ttctattttt gaatctacac aatttcattg ttagaggtga cttaaaagaa gctaaaaaac
     3061 aaagaaatta agagaagttt tttgataata acaaaaatag ggggtgggag cacagggaga
     3121 aagagcatgt agggatgttg aagagtaatt ggacttagaa gtcagaggca cgggcgcgag
     3181 atgctgaaaa tttaaatatt actcgttagt tagtagaact cagtttaaac ttactctgat
     3241 gacagcggcc cctattattt ttgacaccag acaagttggt aatggtagcg accggcgctc
     3301 agttggaatt ctacgaatgc tatttgtata gttcatccat gccatgtgta atcccagcag
     3361 ctgttacaaa ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaaggg
     3421 cagattgtgt ggacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag
     3481 tattttgttg ataatggtct gctagttgaa cgcttccatc ttcaatgttg tgtctaattt
     3541 tgaagttctg aaaatttaaa tcagattagt tagttagtaa aatcatgttt aaacttacaa
     3601 ctttgattcc attcttttgt ttgtctgcca tgatgtatac attgtgtgag ttatagttgt
     3661 attccaattt gtgtccaaga atgtttccat cttctttaaa atcaatacct tttaactcga
     3721 ttctattaac aagggtatca ccttcaaact tgacttcagc acctgaaaat ttaaatatgt
     3781 atggttagtt agtaccgaac tgtttaaact tacgtgtctt gtagttcccg tcatctttga
     3841 aaaatatagt tctttcctgt acataacctt cgggcatggc actcttgaaa aagtcatgcc
     3901 gtttcatatg atctgggtat ctcgagaagc attgaacacc ataacagaaa gtagtgacaa
     3961 gtgttggctg aaaatttaaa taatcagggt tagttagtat atatatgttt aaacttaccc
     4021 atggaacagg tagttttcca gtagtgcaaa taaatttaag ggtaagtttt ccgtatgttg
     4081 catcaccttc accctctcca ctgacagaaa atttgtgccc attaacatca ccatctaatt
     4141 caacaagaat tgggacaact ccagtgaaaa gttcttctcc tttactcatt ttttctaccg
     4201 gtaccctcca agggtcctcc tgaaaatgtt ctatgttatg ttagtatcat tcgaaacata
     4261 cctttgggtc ctttggccaa tcccggggat cctctagagt cgacctgcag gcatgcaagc
     4321 ttatttcatt tccaagttgt tagcgtatcc atcgttgtga gtgatttcag ccatttttaa
     4381 gctctgaaaa tttaaatgag aatagttagt tagtaagatc atgtttaaac ttacagcttg
     4441 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgct
//
