LOCUS       sequence-372998-        8599 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Lentiviral expression plasmid of human MYC, blast selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Cao Z, Budinich KA, Huang H, Ren D, Lu B, Zhang Z, Chen Q, Zhou Y, 
            Huang YH, Alikarami F, Kingsley MC, Lenard AK, Wakabayashi A, 
            Khandros E, Bailis W, Qi J, Carroll MP, Blobel GA, Faryabi RB, Bernt
            KM, Berger SL, Shi J
  TITLE     ZMYND8-regulated IRF8 transcription axis is an acute myeloid 
            leukemia dependency.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3604-3622.e10. doi: 
            10.1016/j.molcel.2021.07.018. Epub 2021 Aug 5.
  PUBMED    34358447
REFERENCE   2  (bases 1 to 8599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.07.018"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3604-3622.e10"
COMMENT     Sequence Label: MYC-PGK-blast
FEATURES             Location/Qualifiers
     source          1..8599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1723..1840
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1909..2120
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2140..2149
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2149..2172
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2218..3537
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     primer_bind     complement(3588..3611)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3950..3969
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      4035..4044
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4041..4439
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    4456..5044
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4509..4529)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4927..4938)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5116..5349
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5427..5548
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5464..5483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5518..5537
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5588..5723
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5743..5762)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5744..5762)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5772..5789)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5772..5788)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5781..5803)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5930..6385
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6017..6036)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6227..6248
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6411..6515
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6516..6584,6585..7376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6734..6753)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7547..8135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8036..8055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8289..8306
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8423..8444
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8459..8464,8465..8482,8483..8489)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8497..8513
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8502..8524
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8521..8537
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8521..8537
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8556..8576
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8558..8576
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccagaccac aattttaaaa gaaaaggggg
     1741 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     1801 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     1861 cagagatcca ctttggcgcc ggctcgagtg gctccggtgc ccgtcagtgg gcagagcgca
     1921 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     1981 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2041 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2101 ggtttgccgc cagaacacag gtgtcgtgac gcgggatccg ccaccatgga ttacaaagac
     2161 gatgacgata agctggattt ttttcgggta gtggaaaacc agcagcctcc cgcgacgatg
     2221 cccctcaacg ttagcttcac caacaggaac tatgacctcg actacgactc ggtgcagccg
     2281 tatttctact gcgacgagga ggagaacttc taccagcagc agcagcagag cgagctgcag
     2341 cccccggcgc ccagcgagga tatctggaag aaattcgagc tgctgcccac cccgcccctg
     2401 tcccctagcc gccgctccgg gctctgctcg ccctcctacg ttgcggtcac acccttctcc
     2461 cttcggggag acaacgacgg cggtggcggg agcttctcca cggccgacca gctggagatg
     2521 gtgaccgagc tgctgggagg agacatggtg aaccagagtt tcatctgcga cccggacgac
     2581 gagaccttca tcaaaaacat catcatccag gactgtatgt ggagcggctt ctcggccgcc
     2641 gccaagctcg tctcagagaa gctggcctcc taccaggctg cgcgcaaaga cagcggcagc
     2701 ccgaaccccg cccgcggcca cagcgtctgc tccacctcca gcttgtacct gcaggatctg
     2761 agcgccgccg cctcagagtg catcgacccc tcggtggtct tcccctaccc tctcaacgac
     2821 agcagctcgc ccaagtcctg cgcctcgcaa gactccagcg ccttctctcc gtcctcggat
     2881 tctctgctct cctcgacgga gtcctccccg cagggcagcc ccgagcccct ggtgctccat
     2941 gaggagacac cgcccaccac cagcagcgac tctgaggagg aacaagaaga tgaggaagaa
     3001 atcgatgttg tttctgtgga aaagaggcag gctcctggca aaaggtcaga gtctggatca
     3061 ccttctgctg gaggccacag caaacctcct cacagcccac tggtcctcaa gaggtgccac
     3121 gtctccacac atcagcacaa ctacgcagcg cctccctcca ctcggaagga ctatcctgct
     3181 gccaagaggg tcaagttgga cagtgtcaga gtcctgagac agatcagcaa caaccgaaaa
     3241 tgcaccagcc ccaggtcctc ggacaccgag gagaatgtca agaggcgaac acacaacgtc
     3301 ttggagcgcc agaggaggaa cgagctaaaa cggagctttt ttgccctgcg tgaccagatc
     3361 ccggagttgg aaaacaatga aaaggccccc aaggtagtta tccttaaaaa agccacagca
     3421 tacatcctgt ccgtccaagc agaggagcaa aagctcattt ctgaagagga cttgttgcgg
     3481 aaacgacgag aacagttgaa acacaaactt gaacagctac ggaactcttg tgcgtgataa
     3541 acgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     3601 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     3661 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     3721 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     3781 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     3841 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcagg
     3901 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
     3961 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
     4021 tgcagcccaa gcttgccacc atggccaagc ctttgtctca agaagaatcc accctcattg
     4081 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     4141 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     4201 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     4261 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     4321 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     4381 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaaa
     4441 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4501 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4561 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4621 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4681 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4741 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4801 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4861 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4921 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4981 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5041 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5101 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5161 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5221 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5281 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5341 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5401 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     5461 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5521 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     5581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5701 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     5761 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5821 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5881 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     5941 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6001 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6061 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6121 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6181 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6241 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6301 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6361 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6421 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6481 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6541 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6601 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6661 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6721 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6781 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6841 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6901 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6961 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7021 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7081 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7141 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7201 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7261 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7321 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7381 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7441 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7501 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7561 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7621 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7681 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7741 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7801 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7861 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7921 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7981 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8041 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8101 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8161 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8221 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8281 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8341 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8401 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8461 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8521 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     8581 aaaagctgga gctgcaagc
//
