LOCUS       sequence-373025-        2837 bp    DNA     circular SYN 14-APR-2025
DEFINITION  BioBrick pSB1C3 plasmid that constitutively overexpresses 
            mRFP1_Orange chromoprotein in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Bao L, Menon PNK, Liljeruhm J, Forster AC
  TITLE     Overcoming chromoprotein limitations by engineering a red 
            fluorescent protein.
  JOURNAL   Anal Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936.
  PUBMED    32891596
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2020 Sep 3:113936. doi: 10.1016/j.ab.2020.113936."
COMMENT     Sequence Label: pSB1C3-J23110-B0034-mRFP1_Orange
FEATURES             Location/Qualifiers
     source          1..2837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        23..57
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             66..77
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             84..761
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFCYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     misc_feature    790..810
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      811..868
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(892..909)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1063..1651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1143..1162)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1834..1928)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1949..2608)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2523..2542
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2770..2788
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2792..2835)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 gaattcgcgg ccgcttctag agtttacggc tagctcagtc ctaggtacaa tgctagctac
       61 tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc
      121 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag
      181 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc
      241 cgctgccgtt cgcttgggac atcctgtccc cgcagttctg ctacggttcc aaagcttacg
      301 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat
      361 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc
      421 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg
      481 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg
      541 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact
      601 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt
      661 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac
      721 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt
      781 gtagatcgct actagtagcg gccgctgcag tccggcaaaa aagggcaagg tgtcaccacc
      841 ctgccctttt tctttaaaac cgaaaagatt acttcgcgtt atgcaggctt cctcgctcac
      901 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
      961 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     1021 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccaca ggctccgccc
     1081 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     1141 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     1201 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     1261 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     1321 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     1381 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     1441 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     1501 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     1561 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     1621 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     1681 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     1741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1801 tgagtaaact tggtctgaca gctcgaggct tggattctca ccaataaaaa acgcccggcg
     1861 gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg gatctatcaa
     1921 caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca tcgcagtact
     1981 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     2041 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     2101 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     2161 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     2221 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     2281 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     2341 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacga aattccggat
     2401 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     2461 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     2521 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     2581 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     2641 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     2701 cgtgcccgat caactcgagt gccacctgac gtctaagaaa ccattattat catgacatta
     2761 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     2821 gctaaggatg atttctg
//
