LOCUS       sequence-37316-e         827 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Subach FV, Subach OM, Gundorov IS, Morozova KS, Piatkevich KD, 
            Cuervo AM, Verkhusha VV
  TITLE     Monomeric fluorescent timers that change color from blue to red 
            report on cellular trafficking.
  JOURNAL   Nat Chem Biol. 2009 Feb;5(2):118-26. Epub 2009 Jan 11.
  PUBMED    19136976
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol."; date: "2009-02"; volume: "5(2)"; pages: "118-26. Epub 2009 
            Jan 11"
COMMENT     Sequence Label: pBAD/HisB-Medium-FT
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..726)
                     /codon_start=1
                     /product="blue-to-red fluorescent timer derivative of 
                     mCherry (Subach et al., 2009)"
                     /label=Medium-FT
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHLEGSVDGHEFEIEGEGEGRPYEG
                     TQSAKLKVTKGGPLPFAWDILSPQFMYGSRAYVKHPADIPDYWKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERIYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVLLPGAYNVNIKMDITSHNEDYTIVEQCERA
                     EGHHSTGGMDELYK"
     primer_bind     complement(294..313)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     563..581
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(join(745..759,760..768))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(772..804)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(808..825)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gccagcttcg aattcttact tgtacagctc gtccatgccg ccggtggaat ggtggccctc
       61 ggcgcgttcg cactgttcaa cgatggtgta gtcctcgttg tgggaggtga tgtccatctt
      121 gatgttgacg ttgtaggcgc cgggcagcag cacgggcttc ttggccttgt aggtggtctt
      181 gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
      241 cagggcgccg tcctcggggt atatccgctc ggaggaggcc tcccagccca tggtcttctt
      301 ctgcattacg gggccgtcgg aagggaagtt ggtgccgcgc agcttcacct tgtagatgaa
      361 ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccat cctcgaagtt
      421 catcacgcgc tcccacttga agccctcggg gaaggacagc ttccagtagt cggggatgtc
      481 ggcggggtgc ttcacgtagg ccctggagcc gtacatgaac tgaggggaca ggatgtccca
      541 ggcgaagggc agggggccac ccttggtcac cttcagcttg gcgctctggg tgccctcgta
      601 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgtccacgg agccctccag
      661 gtgcaccttg aaacgcatga attccttgat gatggccatg ttatcctcct cgcccttgct
      721 taccatagat ctcgagctcg gatccttatc gtcatcgtcg tacagatccc gacccatttg
      781 ctgtccacca gtcatgctag ccataccatg atgatgatga tgatgag
//
