LOCUS       sequence-373250-       10086 bp    DNA     circular SYN 15-APR-2025
DEFINITION  EGFP fused to the C-terminus of OGG1 and a hygromycin resistance 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10086)
  TITLE     Mechanisms of oxidative damage repair
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLV-Hygro-CMV-hOGG1/EGFP
FEATURES             Location/Qualifiers
     source          1..10086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..25
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9..25
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     44..64
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        46..64
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        92..318
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             319..499
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    546..671
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1164..1397
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1582..1626
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1892..2009
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2027..2047
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2108..2411
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2412..2615
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2566..2586
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2636..2660
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3702..3704,3705..3707,3708..4421)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3702..3704,3705..3707,3708..4418)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3747..3768)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4008..4027)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4355..4376
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4422..4446)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4464..5052
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4517..4537)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4935..4946)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5083..5582
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5118..5141)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5515..5534
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5599..6624
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5599..6624
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             6696..6929
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7001..7122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7038..7057)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7092..7111
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7162..7297
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7317..7336)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7318..7336)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7346..7363)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7346..7362)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7355..7377)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7504..7959
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7591..7610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7801..7822
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7985..8089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8090..8158,8159..8950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8308..8327)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9121..9709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9610..9629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9863..9880
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9997..10018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10033..10038,10039..10056,10057..10063)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    join(10071..10086,1)
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(10076..10086,1..12)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
       61 aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct
      121 tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca
      181 tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt
      241 ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc
      301 ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
      361 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
      421 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
      481 gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag
      541 aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc
      601 ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt
      661 gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa
      721 ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag
      781 aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg
      841 gacagctaca accatccctt cagacaggat cagaagaact tagatcatta tataatacag
      901 tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag
      961 acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc
     1021 ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa
     1081 gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag
     1141 agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga
     1201 agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt
     1261 atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa
     1321 ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta
     1381 aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct
     1441 gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc
     1501 tggatggagt gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa
     1561 gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca
     1621 agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg
     1681 atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga
     1741 gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc
     1801 gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag atccattcga
     1861 ttagtgaacg gatctcgacg gtatcgctag cttttaaaag aaaagggggg attggggggt
     1921 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     1981 aaaaacaaat tacaaaaatt caaaatttta ctagtgatta tcggatcaac tttgtataga
     2041 aaagttgtag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     2101 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     2161 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     2221 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     2281 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     2341 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     2401 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     2461 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     2521 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     2581 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccaag
     2641 tttgtacaaa aaagcaggct gccaccatgc ctgcccgcgc gcttctgccc aggcgcatgg
     2701 ggcatcgtac tctagcctcc actcctgccc tgtgggcctc catcccgtgc cctcgctctg
     2761 agctgcgcct ggacctggtt ctgccttctg gacaatcttt ccggtggagg gagcaaagtc
     2821 ctgcacactg gagtggtgta ctagcggatc aagtatggac actgactcag actgaggagc
     2881 agctccactg cactgtgtac cgaggagaca agagccaggc tagcaggccc acaccagacg
     2941 agctggaggc cgtgcgcaag tacttccagc tagatgttac cctggctcaa ctgtatcacc
     3001 actggggttc cgtggactcc cacttccaag aggtggctca gaaattccaa ggtgtgcgac
     3061 tgctgcgaca agaccccatc gaatgccttt tctcttttat ctgttcctcc aacaacaaca
     3121 tcgcccgcat cactggcatg gtggagcggc tgtgccaggc ttttggacct cggctcatcc
     3181 agcttgatga tgtcacctac catggcttcc ccagcctgca ggccctggct gggccagagg
     3241 tggaggctca tctcaggaag ctgggcctgg gctatcgtgc ccgttacgtg agtgccagtg
     3301 cccgagccat cctggaagaa cagggcgggc tagcctggct gcagcagcta cgagagtcct
     3361 catatgagga ggcccacaag gccctctgca tcctgcctgg agtgggcacc aaggtggctg
     3421 actgcatctg cctgatggcc ctagacaagc cccaggctgt gcccgtggat gtccatatgt
     3481 ggcacattgc ccaacgtgac tacagctggc accctaccac gtcccaggcg aagggaccga
     3541 gcccccagac caacaaggaa ctgggaaact ttttccggag cctgtgggga ccttatgctg
     3601 gctgggccca agcggtgctg ttcagtgccg acctgcgcca atcccgccat gctcaggagc
     3661 caccagcaaa gcgcagaaag ggttccaaag ggccggaagg catggtgagc aagggcgagg
     3721 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3781 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3841 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3901 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3961 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     4021 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4081 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     4141 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     4201 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     4261 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     4321 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     4381 ccgccgggat cactctcggc atggacgagc tgtacaagta aacccagctt tcttgtacaa
     4441 agtggtgata atcgaattcc gataatcaac ctctggatta caaaatttgt gaaagattga
     4501 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     4561 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4621 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     4681 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     4741 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     4801 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     4861 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     4921 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4981 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5041 ccgcctcccc gcatcgggaa ttcccgcggt tcgaattcta ccgggtaggg gaggcgcttt
     5101 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     5161 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     5221 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     5281 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     5341 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     5401 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     5461 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     5521 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     5581 cgacctcacg tggccaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt
     5641 ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct
     5701 cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc
     5761 gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt
     5821 ccggaagtgc ttgacattgg ggaatttagc gagagcctga cctattgcat ctcccgccgt
     5881 gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg
     5941 gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc
     6001 ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt
     6061 gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc
     6121 gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc
     6181 gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc
     6241 attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc
     6301 tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg
     6361 gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc
     6421 tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac
     6481 gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg
     6541 gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc
     6601 actcgtccga gggcaaagga atagggtacc tttaagacca atgacttaca aggcagctgt
     6661 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6721 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     6781 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     6841 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     6901 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     6961 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     7021 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7081 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     7141 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7201 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7261 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     7321 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     7381 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     7441 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     7501 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     7561 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     7621 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7681 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     7741 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7801 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     7861 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     7921 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     7981 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8041 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8101 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8161 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8221 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8281 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8341 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8401 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8461 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8521 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8581 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8641 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8701 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8761 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8821 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8881 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8941 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9001 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9061 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9121 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9181 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9241 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9301 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9361 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9421 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9481 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaaga
     9541 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9601 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9661 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9721 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9781 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9841 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9901 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9961 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
    10021 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
    10081 ataaca
//
