LOCUS       sequence-373325-        8908 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa),
            in mammalian cells .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8908)
  AUTHORS   Abu Rmaileh A, Solaimuthu B, Khatib A, Lavi S, Tanna M, Hayashi A, 
            Ben Yosef M, Lichtenstein M, Pillar N, Shaul YD
  TITLE     DPYSL2 interacts with JAK1 to mediate breast cancer cell migration.
  JOURNAL   J Cell Biol. 2022 Jul 4;221(7). pii: 213220. doi: 
            10.1083/jcb.202106078. Epub 2022 May 16.
  PUBMED    35575798
REFERENCE   2  (bases 1 to 8908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-07-4"; volume: "221(7). pii: 213220. doi"; 
            pages: " 10.1083/jcb.202106078. Epub 2022 May 16"
COMMENT     Sequence Label: ∆C' terminal-DPYSL2-pLJC2
FEATURES             Location/Qualifiers
     source          1..8908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1748..1813
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    1861..1978
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2027..2537
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2456..2474
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2559..3158
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2559..2578)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3055..3075
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             3286..3519
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3591..3712
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3628..3647)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3682..3701
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3752..3887
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3907..3926)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3908..3926)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3936..3953)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3936..3952)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3945..3967)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4094..4549
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4181..4200)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4391..4412
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4575..4679
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4680..4748,4749..5540)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4898..4917)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5711..6299
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6200..6219
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6453..6470
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6587..6608
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6623..6628,6629..6646,6647..6653)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6661..6677
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6666..6688
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6685..6701
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6685..6701
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6720..6740
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6722..6740
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6768..6994
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6995..7175
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7222..7347
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7840..8073
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8258..8302
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     8600..8620
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tttaattaag agagagatgt cttatcaggg gaagaaaaat attccacgca tcacgagcga
      301 tcgtcttctg atcaaaggag gtaaaattgt taatgatgac cagtcgttct atgcagacat
      361 atacatggaa gatgggttga tcaagcaaat aggagaaaat ctgattgtgc caggaggagt
      421 gaagaccatc gaggcccact cccggatggt gatccccgga ggaattgacg tccacactcg
      481 tttccagatg cctgatcagg gaatgacgtc tgctgatgat ttcttccaag gaaccaaggc
      541 ggccctggct gggggaacca ctatgatcat tgaccacgtt gttcctgagc ctgggacaag
      601 cctgctcgct gcctttgacc agtggaggga atgggccgac agcaagtcct gctgtgacta
      661 ctctctgcat gtggacatca gcgagtggca taagggcatc caggaggaga tggaagcgct
      721 tgtgaaggat cacggggtaa attccttcct cgtgtacatg gctttcaaag atcgcttcca
      781 gctaacggat tgccagattt atgaagtact gagtgtgatc cgggatattg gcgccatagc
      841 ccaagtccac gcagaaaatg gcgacatcat tgcagaggag cagcagagga tcctggatct
      901 gggcatcacg ggccccgagg gacatgtgct gagccgacct gaggaggtcg aggccgaagc
      961 cgtgaatcgt gccatcacca tcgccaacca gaccaactgc ccgctgtata tcaccaaggt
     1021 gatgagcaaa agctctgctg aggtcatcgc ccaggcacgg aagaagggaa ctgtggtgta
     1081 tggcgagccc atcactgcca gcttgggaac ggacggctcc cattactgga gcaagaactg
     1141 ggccaaggct gctgcctttg tcacctcccc acccttgagc cctgatccaa ccactccaga
     1201 ctttctcaac tccttgctgt cctgtggaga cctccaggtc acgggcagtg cccattgcac
     1261 gtttaacact gcccagaagg ctgtaggaaa ggacaacttc accctgattc cggagggcac
     1321 caatggcact gaggagcgga tgtccgtcat ctgggacaag gctgtggtca ctgggaagat
     1381 ggatgagaac cagtttgtgg ctgtgaccag caccaatgca gccaaagtct tcaaccttta
     1441 cccccggaaa ggccgcattg ctgtgggatc cgatgccgac ctggtcatct gggaccccga
     1501 cagcgttaaa accatctctg ccaagacaca caacagctct ctcgagtaca acatctttga
     1561 aggcatggag tgccgcggct ccccactggt ggtcatcagc caggggaaga ttgtcctgga
     1621 ggacggcacc ctgcatgtca ccgaaggctc tggacgctac attccccgga agcccttccc
     1681 tgattttgtt tacaagcgta tcaaggcaag gagcaggctg gctgagggag gcagcgcggc
     1741 cgcaggcgac tacaaagacc atgacggtga ttataaagat catgacattg attacaagga
     1801 tgacgatgac aagtaataag aattctcgac ctcgagacaa atggcagtat tcatccacaa
     1861 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     1921 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     1981 ggtttattac agggacagca gagatccact ttggccgcgg ctcgaggggg ttggggttgc
     2041 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     2101 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     2161 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     2221 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     2281 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     2341 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     2401 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     2461 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     2521 ccgacctctc tccccagggg gatccaccgg agcttaccat gaccgagtac aagcccacgg
     2581 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     2641 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     2701 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     2761 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     2821 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     2881 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     2941 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     3001 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     3061 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     3121 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     3181 gcccgaccga aaggagcgca cgaccccatg catcggtacc tttaagacca atgacttaca
     3241 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     3301 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     3361 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     3421 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     3481 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
     3541 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     3601 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     3661 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     3721 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     3781 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     3841 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     3901 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     3961 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     4021 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     4081 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     4141 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     4201 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     4261 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     4321 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4381 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4441 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4501 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     4561 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4621 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4681 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4741 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4801 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4861 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4921 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4981 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     5041 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     5101 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     5161 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5221 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5281 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5341 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5401 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5461 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5521 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5581 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5641 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5701 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5761 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5821 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5881 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5941 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     6001 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     6061 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     6121 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6181 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6241 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6301 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6361 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6421 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6481 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     6541 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     6601 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     6661 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     6721 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     6781 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     6841 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     6901 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     6961 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     7021 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     7081 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     7141 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     7201 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     7261 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     7321 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     7381 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     7441 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     7501 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     7561 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     7621 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     7681 caagcagcag ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     7741 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     7801 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     7861 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     7921 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     7981 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     8041 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     8101 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     8161 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     8221 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8281 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8341 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     8401 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8461 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     8521 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     8581 agcagcagcc cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg
     8641 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     8701 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     8761 ttttaaaatg gactatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     8821 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     8881 ctacgtatta gtcatcgcta ttaccatg
//
