LOCUS       sequence-374370-       12455 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Protein expression ADAM10 in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12455)
  TITLE     TREAT-AD plasmids 2022
REFERENCE   2  (bases 1 to 12455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSCM167.2-Venus-VF-hADAM10
FEATURES             Location/Qualifiers
     source          1..12455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     1297..1316
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1297..1315
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(1332..1334,1335..1337,1338..2048)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1377..1398)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1638..1657)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1985..2006
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2088..2111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    2133..2157
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4416..4440)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4459..4477)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4460..4477)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    4526..5114
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4579..4599)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4997..5008)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5117..5133)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5118..5134)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5639..5819
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5845..5862)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5851..6075
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6121..6549
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6208..6227)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6418..6439
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6558..6578)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6563..6892
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6743..6878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6805..6824
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6940..6987
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7006..7380
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7510..7631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7547..7566)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7601..7620
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7680..7696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7680..7696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7693..7715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7704..7720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7728..7734,7735..7752,7753..7758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7773..7794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7911..7928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8082..8670)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8162..8181)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8841..9632,9633..9701))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9464..9483
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9702..9806)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9881..9900)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10072..10451
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10453..10651
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10606..10626
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             10669..10849
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10896..11021
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11514..11747
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11932..11976
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12274..12391
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga ggatccagtt ctctctggct
     1261 aactagagaa cccactgctt actggcttat cgaaattaat acgactcact atagggagac
     1321 ccaagcttac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     1381 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     1441 atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     1501 cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     1561 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     1621 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     1681 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     1741 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc accgccgaca
     1801 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     1861 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     1921 ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac gagaagcgcg
     1981 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     2041 tgtacaaggg agggggtgga tctggagggg gtggatctaa gcttatggac tacaaggacg
     2101 acgatgacaa gggtggtgga tccgaattat caacaagttt gtacaaaaaa gcaggcttcg
     2161 aaggagatag aaccatggcc gtgttgctga gagtgttaat tctgctcctc tcctgggcgg
     2221 cggggatggg aggtcagtat gggaatcctt taaataaata tatcagacat tatgaaggat
     2281 tatcttacaa tgtggattca ttacaccaaa aacaccagcg tgccaaaaga gcagtctcac
     2341 atgaagacca attttcacgt ctagatttcc atgcccatgg aagacatttc aacctacgaa
     2401 tgaagaggga cacttccctt ttcagtgatg aatttaaagt agaaacatca aataaagtac
     2461 ttggttatga tacctctcat atttacactg gacatattta tggtgaagaa ggaagttttg
     2521 gccatgggtc tgttattgat ggaagatttg aaggattcat ccagactcgt ggtggcacat
     2581 tttatgttga gccagcagag agatatatta aagaccgaac tctgccattt cactctgtca
     2641 tttatcatga agatgatatt aactatcccc ataaatacgg tcctcagggg ggctgtgcag
     2701 atcattcagt atttgaaaga atgaggaaat accagatgac tggtgtagag gaagtaacac
     2761 agatacctca agaagaacat gctgctaatg gtccagaact tctgaggaaa aaacgtacaa
     2821 cttcagctga aaaaaatact tgtcagcttt atattcagac tgatcatttg ttctttaaat
     2881 attacggaac acgagaagct gtgattgccc agatatccag tcatgttaaa gcgattgata
     2941 caatttacca gaccacagac ttctccggaa tccgtaacat cagtttcatg gtgaaacgca
     3001 taagaatcaa tacaactgct gatgagaagg accctacgaa tcctttccgt ttcccaaata
     3061 ttggtgtgga gaagtttctg gaattgaatt ctgagcagaa tcatgatgac tactgtttgg
     3121 cccatgtctt cacagaccga gattttgatg atggcgtact tggtctggct tgggttggag
     3181 caccttcagg aagctctgga ggaatatgtg aaaaaagtaa actctattca gatggtaaga
     3241 agaagtcctt aaacactgga attattactg ttcagaacta tgggtctcat gtacctccca
     3301 aagtctctca cattactttt gctcacgaag ttggacataa ctttggatcc ccacatgatt
     3361 ctggaacaga gtgcacacca ggagaatcta agaatttggg tcaaaaagaa aatggcaatt
     3421 acatcatgta tgcaagagca acatctgggg acaaacttaa caacaataaa ttctcactct
     3481 gtagtattag aaatataagc caagttcttg agaagaagag aaacaactgt tttgttgaat
     3541 ctggccaacc tatttgtgga aatggaatgg tagaacaagg tgaagaatgt gattgtggct
     3601 atagtgacca gtgtaaagat gaatgctgct tcgatgcaaa tcaaccagag ggaagaaaat
     3661 gcaaactgaa acctgggaaa cagtgcagtc caagtcaagg tccttgttgt acagcacagt
     3721 gtgcattcaa gtcaaagtct gagaagtgtc gggatgattc agactgtgca agggaaggaa
     3781 tatgtaatgg cttcacagct ctctgcccag catctgaccc taaaccaaac ttcacagact
     3841 gtaataggca tacacaagtg tgcattaatg ggcaatgtgc aggttctatc tgtgagaaat
     3901 atggcttaga ggagtgtacg tgtgccagtt ctgatggcaa agatgataaa gaattatgcc
     3961 atgtatgctg tatgaagaaa atggacccat caacttgtgc cagtacaggg tctgtgcagt
     4021 ggagtaggca cttcagtggt cgaaccatca ccctgcaacc tggatcccct tgcaacgatt
     4081 ttagaggtta ctgtgatgtc ttcatgcggt gcagattagt agatgctgat ggtcctctag
     4141 ctaggcttaa aaaagcaatt tttagtccag agctctatga aaacattgct gaatggattg
     4201 tggctcattg gtgggcagta ttacttatgg gaattgctct gatcatgcta atggctggat
     4261 ttattaagat atgcggtgtt catactccaa gtagtaatcc aaagctgcct cctcctaaac
     4321 cacttccagg cactttaaag aggaggagac ctccacagcc cattcagcaa ccccagcgtc
     4381 agcggccccg agagagttat caaatgggac acatgagacg cgtttcttgt acaaagtggt
     4441 tgatctagag ggccctattc tatagtgtca cctaaatgct agagaacctc gagggcgcgc
     4501 cgaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     4561 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     4621 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     4681 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     4741 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     4801 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     4861 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     4921 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     4981 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     5041 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     5101 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     5161 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     5221 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     5281 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     5341 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     5401 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     5461 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     5521 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     5581 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     5641 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5701 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5761 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5821 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5881 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5941 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6001 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6061 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     6121 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6181 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6241 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6301 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6361 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6421 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6481 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6541 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6601 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6661 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6721 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6781 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     6841 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6901 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     6961 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     7021 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     7081 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     7141 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     7201 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     7261 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     7321 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     7381 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7441 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     7501 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7621 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     7681 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7741 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7801 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7861 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7921 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7981 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8041 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8101 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8161 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8221 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8281 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8341 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8401 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8461 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8521 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8581 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8641 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8701 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8761 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8821 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8881 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8941 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     9001 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     9061 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     9121 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     9181 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9241 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9301 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9361 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9421 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9481 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9541 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9601 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9661 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9721 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9781 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     9841 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     9901 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     9961 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    10021 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
    10081 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    10141 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    10201 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    10261 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    10321 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    10381 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    10441 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
    10501 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
    10561 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
    10621 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg gtctctctgg
    10681 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    10741 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    10801 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
    10861 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
    10921 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
    10981 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
    11041 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
    11101 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
    11161 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
    11221 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
    11281 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
    11341 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
    11401 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
    11461 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
    11521 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
    11581 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
    11641 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
    11701 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
    11761 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
    11821 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    11881 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    11941 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    12001 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    12061 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    12121 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    12181 ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact gcgtgcgcca
    12241 attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
    12301 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
    12361 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
    12421 agtttggtta attaacccgt gtcggctcca gatct
//
