LOCUS       sequence-374858-        9279 bp    DNA     circular SYN 15-APR-2025
DEFINITION  sgRNAs targeting enhancer 1 and 4 of MYC separately.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9279)
  AUTHORS   Lin X, Liu Y, Liu S, Zhu X, Wu L, Zhu Y, Zhao D, Xu X, Chemparathy 
            A, Wang H, Cao Y, Nakamura M, Noordermeer JN, La Russa M, Wong WH, 
            Zhao K, Qi LS
  TITLE     Nested epistasis enhancer networks for robust genome regulation.
  JOURNAL   Science. 2022 Aug 11:eabk3512. doi: 10.1126/science.abk3512.
  PUBMED    35951677
REFERENCE   2  (bases 1 to 9279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2022 Aug 11:eabk3512. doi: 10.1126/science.abk3512."
COMMENT     Sequence Label: double_sgRNA targeting e1 and e4
FEATURES             Location/Qualifiers
     source          1..9279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2..18)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             527..707
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(769..1357)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(849..868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1528..2319,2320..2388))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2151..2170
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2389..2493)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2568..2587)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2759..3138
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3140..3338
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3293..3313
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3356..3536
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3583..3708
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4205..4438
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4623..4667
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4965..5082
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(5335..5368)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     5372..5392
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     5651..5671
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5822..5841
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5920..5995
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        6020..7198
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6251..7189
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7146..7166
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             7210..7806
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7210..7229)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7706..7726
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             7816..7869
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7882..8580
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     protein_bind    complement(8600..8633)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    8689..9277
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8742..8762)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9160..9171)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
       61 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
      121 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
      181 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
      241 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
      301 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
      361 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
      421 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
      481 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
      541 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      601 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      661 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa
      721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
      781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
      841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
      901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
      961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1201 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     1501 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     1561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     1621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     1681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     1741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     1801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     1861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     1921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     1981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2401 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     2461 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     2521 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
     2581 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
     2641 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     2701 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
     2761 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     2821 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     2881 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     2941 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     3001 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     3061 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     3121 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     3181 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     3241 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     3301 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
     3361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     3421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     3481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     3541 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     3601 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     3661 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     3721 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     3781 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     3841 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     3901 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     3961 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     4021 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
     4081 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     4141 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     4201 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     4261 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     4321 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     4381 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     4441 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     4501 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     4561 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     4621 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     4681 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     4741 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     4801 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
     4861 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
     4921 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
     4981 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     5041 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     5101 agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc gccatctcta
     5161 ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc ccctgccccg
     5221 gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag
     5281 ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat ttctataact
     5341 tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca ccctaactgt
     5401 aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt gttggaacct
     5461 tcagggaaaa ggcttgttta agagctaagc tggaaacagc atagcaagtt taaataaggc
     5521 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct cgagtactag
     5581 gatccattag gcggccgcgt ggataaccgt attaccgcca tgcagccttt tgctcattaa
     5641 ttaaggtacc gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     5701 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     5761 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     5821 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     5881 gtggaaagcc agaaacatgg gctggctggg tctggtagtg ttttagagct agaaatagca
     5941 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     6001 ttgaattcgc tagcctagag gtacctgtgc ccgtcagtgg gcagagcgca catcgcccac
     6061 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     6121 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     6181 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     6241 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     6301 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     6361 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     6421 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     6481 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     6541 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     6601 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     6661 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     6721 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     6781 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     6841 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     6901 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     6961 gccgtccagg cacctcgatt agttctcgcg cttttggagt acgtcgtctt taggttgggg
     7021 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     7081 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     7141 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagc
     7201 tagcccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     7261 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     7321 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     7381 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     7441 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     7501 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     7561 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     7621 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     7681 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     7741 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     7801 ggtgccggat cgggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
     7861 cctggcccac cggtcgccac cagcgagctg attaaggaga acatgcacat gaagctgtac
     7921 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
     7981 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
     8041 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
     8101 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
     8161 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
     8221 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
     8281 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
     8341 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc
     8401 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
     8461 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
     8521 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaattaa
     8581 gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt
     8641 taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg
     8701 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     8761 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     8821 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     8881 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     8941 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     9001 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     9061 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     9121 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     9181 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     9241 cagacgagtc ggatctccct ttgggccgcc tccccgcat
//
