LOCUS       sequence-37492-e         953 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Subach FV, Patterson GH, Manley S, Gillette JM, Lippincott-Schwartz 
            J, Verkhusha VV
  TITLE     Photoactivatable mCherry for high-resolution two-color fluorescence 
            microscopy.
  JOURNAL   Nat Methods. 2009 Feb;6(2):153-9. Epub 2009 Jan 25.
  PUBMED    19169259
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2009-02"; volume: "6(2)"; pages: "153-9. Epub 2009
            Jan 25"
COMMENT     Sequence Label: pPAmCherry3-C1
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..753
                     /codon_start=1
                     /product="photoactivatable monomeric derivative of DsRed 
                     fluorescent protein"
                     /label=PAmCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHLEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFTWDILSPQFMYGSNAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVIQKKTMGWDALSERMYPEDGALK
                     GELKARLKLKDGGHYEAEVKTTYKAKKPVQLPGAYNVNRKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(191..209)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    754..819
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gcgagctggt ttagtgaacc gtcagatccg ctagcggtcg ccaccatggt gagcaagggc
       61 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca cctggagggg
      121 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggt
      181 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcac ctgggacatc
      241 ctgtcccctc aattcatgta cggctccaat gcctacgtga agcaccccgc cgacatcccc
      301 gactacttta agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      361 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatatac
      421 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaataca gaagaagacc
      481 atgggctggg atgccctctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      541 ctcaaggcga ggctgaagct gaaggacggc ggccactatg aggctgaggt caagaccaca
      601 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaatcg caagttggat
      661 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgtgc cgagggccgc
      721 cactccaccg gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct
      781 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc
      841 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
      901 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tat
//
