LOCUS       sequence-375280-       11373 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Prig-3-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans AVA
            neuron expression of GtACR2 RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11373)
  AUTHORS   Lu Y, Ahamed T, Mulcahy B, Meng J, Witvliet D, Guan SA, Holmyard D, 
            Hung W, Wen Q, Chisholm AD, Samuel ADT, Zhen M
  TITLE     Extrasynaptic signaling enables an asymmetric juvenile motor circuit
            to produce symmetric undulation.
  JOURNAL   Curr Biol. 2022 Nov 7;32(21):4631-4644.e5. doi: 
            10.1016/j.cub.2022.09.002. Epub 2022 Sep 30.
  PUBMED    36182701
REFERENCE   2  (bases 1 to 11373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2022.09.002"; journalName: "Curr Biol"; date: 
            "2022-11-7- 7"; volume: "32"; issue: "21"; pages: "4631-4644.e5"
COMMENT     Sequence Label: pJH4217
FEATURES             Location/Qualifiers
     source          1..11373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(4939..4972)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      5849..6172
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    complement(6176..6209)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8919..8941)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9041..9060
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9138..9156)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9224..9328
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9329..9397,9398..10189)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9547..9566)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10360..10948
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10849..10868
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11102..11119
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11236..11257
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11272..11277,11278..11295,11296..11302)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11310..11326
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11315..11337
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11334..11350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11334..11350
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttaaacatga tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct
       61 gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaataagc ttgggctgca
      121 gcatgcggcc gctcgtcttt accgctttgt tttttattgc ttactttacc tctagtgatt
      181 atcttattgg aatttatgca catgacgtgt tgtactagtt taacaatttc aattgacagt
      241 tttttcgatt aggcttttga acatatttat atatttcttt atttacgaat gtgaacttaa
      301 aagtggattc tcgaaatctt ttatcgggtt ttttgagcct aaaatgaccc atttatcaag
      361 atttatcaaa aaagctatga cggtttaaag gtttttgatt aaaggcgtgt tttttttagc
      421 taaaatctca aactctgcta aactatcagt gccccaacgt gaaaaaacaa catgaaaaac
      481 cccaaaatca aaaagcatct taatcagcca aaaacacttc ataaattgca aaaatatgca
      541 ttaacaaatg acaactaaat ggaaaagtac tcccgacttt tcaagatgga aaaatgtgag
      601 atcttcgctg aaaatagagt tttttttcaa atgaaaaatt tggaataaag ctcaaatgtt
      661 tgtctcaaag ttggtaattg ttgaaaaaaa ttttgataat ttggcgaata tttgtgaatt
      721 gaaacataac tatttcggtt ttacgcgtac ttgtaaaatt caaaatttta caagaaatta
      781 taaaacttta tgagctttag atggtcaata cgcaaacatt catctagttt atagccttac
      841 attaaaacat tttacctatt caggttaatt gaacagtatc tttaaaggtg gtgtagtcga
      901 attttttaaa atttctttat tagactcaaa attgtctgaa aacaccaaat ttcataagta
      961 aacttcttga aaacttttcg gctcgaaaaa atgacctaaa attagtgaaa atttgaaatg
     1021 tggccaattt atcaagcggc tgcaaacaat ttttttttga aattgttgtc tgattttgga
     1081 tatagatgta aggtatttaa aaaaaagatg gacgaagaaa attggtaaaa aaatttgaca
     1141 aatctcttcg tccatcgact ttgaaagacc ttaatcaagt ttaaaacgca agatagtcaa
     1201 tttatatacc caaaatcaga tgataattca aaaaaaattg tttccagccg ctgcgacaat
     1261 gttaagttaa tttaaggtca tttttcgagc agccataact tctttttttt aagtttcaac
     1321 aagtttcatt ataaaattcg gtgttttcag acaattttga atataataaa gaaataaaga
     1381 aaaattcgac tacatcacct ttaaactaaa tttcaaatgt aatttttttt tacttttcaa
     1441 aatgattcgt tctcggtttt gttgtgaaca tttttttgct aatacgctta cgaaatgttt
     1501 tgtgtggcaa tcattttgat tgaagttccc ggcagctaca acatattttt agaactagaa
     1561 aacaatagaa cgcacagaat atccacgttc ccatgttttc aatttctctt ttcgaaatcc
     1621 tttacaaaat ccgaacgccg ttctgattca gccttgagac atttttcaaa aaaaaaatca
     1681 gtgtgagcat tatagttttc agttttgaat attcacattt tgcacttttt gcctactttc
     1741 cgtattctgc agtttccaca tcaccttgtt acatctctca ggaagtttct gaaagtgaca
     1801 ccacgctcac acacgatgaa attcataccc accaacctcc cttctctgcc tccctcaact
     1861 tcttccacgg aattctatct cctcttatcc agttagggac acatatcgag tttcaccaaa
     1921 aataatgaga agtgtggtca gagacaccac attgaattaa ctattagttc aaaagttgca
     1981 ggtttcctag tgcaaaaagt ttatgttccc atctaccaaa cgactcaccg ccaattgact
     2041 ttgcagtggc aacattggga aatgcgaccc atcatattgt tcctattgtc atgtacaagc
     2101 tgtccctgtt ttgctcgctt aatccgtcaa ccccttcccc gttctaatta gagatgagtg
     2161 aattacaata gagttgaaac ggaaatctga gtgtctgtag ctttccgccg ccgcttttgt
     2221 tacgagatgg tagacgtccc tttcttggtg tttattgtct cacaaactgt ataataaagc
     2281 accgttgtgt aaaaggcatt tggtatcagg aagataaaat gaataaaatg caaatgcgga
     2341 atcaaaaatt gccaaataat tttgaccaat aaaatatcaa aaataataga acagaaataa
     2401 acaaataaaa ctacggagaa aatgtgatta ttccattaaa caacgtttta aaccaacttt
     2461 attcgtcagt ggggaaggtt ttggacaaat atcatagtaa aacttgttcg ttttaattat
     2521 acataatcaa taattatagg tgttagatcc aatggtcgaa atttaaaaaa aaatcaacat
     2581 tttagaaaat ttcggcaatt taccgaaata ttgactgaca attttacata tataagtgtt
     2641 ttgaaataga atccccattg acgatgcgcc accttaaaca gtagaactgt tccaatcaga
     2701 aactaaataa atacggttat tatatatata tatatatata tataacttgt ttttattatg
     2761 catttctcaa aactcaccaa actcaatctc taatttacgg ttgttgagga aaatatattt
     2821 atcacattta gttgagactc taaaagaagt acaacttttt tcttgcaatt cctccaccaa
     2881 tccaattttt caagagtcaa ttgcttggag tcctcgtttt ctcgtaatcc gatattcccc
     2941 ctatttctgt gattttattc agtttcgtcc tcttacacca aaaatcttca tgactttttt
     3001 ggttttctgc ttaatctttt ttttgttttt aaagtctacc atccggcaat tttttttgca
     3061 aatggttgct cagaatttga cctcagatta aaattttcag gaacttcctg gtcccatttc
     3121 gtcacattta ataacaaacc aaatccttca gcttgagtcg tccaagccaa cgcaacccta
     3181 attcgattcc acctccaatt tacctttttg ccgttttcga tcaaattgtg ggggctcttt
     3241 ttatttgaaa gtcgtaaaat ggcgcgtcct tgtcgaaaat tgagatagct aatggttcca
     3301 aataaaatca aatgcatgaa cctagtagga aaaatcgttt tttttttcat cttccaaaga
     3361 aaaagtttaa gataaaccca ccactccagc aatccaccta cgcgtttttc accggaaact
     3421 gtcatttgtt tttgatgtat gacagtgcca gcactgaact aaattcccat ttgttttgtt
     3481 gtggtgttat ctaaccacgt ggctctttct ctaaattttg cgtaggctgc aagatccgca
     3541 atcccaccaa aaaatggttt ctaatgtact tcatacatat cccacatgac tcagtgctcc
     3601 gaaacgccca gcaacccccg cgtttgtctt tacaaaaatg aggcggaaat gggaaatttt
     3661 cctgctattg ctatggtaaa atacttaagc tgtattgaga tttgggaaat tgaaagataa
     3721 gatgaaaaat ccactctgta taattaaaaa ggtttatttt ttttgtaatt tttgtaccgc
     3781 aaattttttc attaactgat ttttctttat tcattcgatg gcccagtttc agatcttcat
     3841 tatcatgtag tttattcatt caaactattt gtgacatcat gatggttgtt taaaaataat
     3901 ttcgaatgaa gaaagcttag acctattata attttctttt ctataaaaaa tattgatttt
     3961 gtaaagcgtt tggcagtgac agtaccaaaa aatgttttaa aatgatttaa gaaaattttt
     4021 tattttattt gtataatacg cgtaaaatga gtatactcgt catttgtcac gaatcgagtt
     4081 ctcagtttgc tgtgaatatc ttgtaataga aatcgatctc atagtgtcac ttttgaggca
     4141 taacttttca ggttgttaca agttcattaa cttgtcaaga ctgggtcatg ttttaaagtg
     4201 taactgttac ccatgtaccc taaactttga gacaaactcc tcaaaacttg taaccagaat
     4261 cctatgtaga tgactcacaa aaccctgttt tcaatttgaa cgaatttcga aactggccaa
     4321 aatgttcccc tcttgctcac ctaatgcttt taccatttaa aaaggatggt aacaacactc
     4381 tttcgaaaat gtaggcggtc cattttcggt ttctcataac cgtctggctc tatctcctag
     4441 tgcgcgagga gagggcgggc ggagtaggaa tcgggggcag cgcactcata cactctgccc
     4501 ttctcttttg cacacacaga aaaagataga tacagaccaa cacttattgt tgtccagtaa
     4561 gcttccagaa aactattttc agaattacaa actacatttt tctagaacag tcaattgatt
     4621 ttttacaaga ttcgagtatt ctaacattag tgaaagtatt tagccactgc aattttcaac
     4681 tgtaggtttc aacttccaaa aaaaattcct ggcatttcta attggatgaa aaaaggctat
     4741 ctagaccctc cattatttac tcataatttc atctcgtcct catttttaat cccctcattg
     4801 atcccatcac aatccgccaa aatgtttttt aaacactttt ttcttcacca gccgtatgtg
     4861 gtcaactgcc taaaaatttc acagctattt gtcattcctt gcagaagaac ttcattcttc
     4921 tttttcgaaa atcccgggat aacttcgtat agcatacatt atacgaagtt atatggtctc
     4981 caagggagag gagctcttca ccggagtcgt cccaatcctc gtcgagctcg acggagacgt
     5041 caacggacac aagttctccg tccgtggaga gggagaggga gacgccacca acggaaagct
     5101 caccctcaag ttcatctgca ccaccggaaa gctcccagtc ccagtaagtt taaacatata
     5161 tatactaact aaccctgatt atttaaattt tcagtggcca accctcgtca ccaccctctc
     5221 ccacggagtc cagtgcttcg cccgttaccc agaccacatg aagcagcacg acttcttcaa
     5281 gtccgccatg ccagagggat acgtccagga gcgtaccatc ttcttcaagg acgacggaac
     5341 ctacaagagt aagtttaaac atgattttac taactaacta atctgattta aattttcagc
     5401 ccgtgccgag gtcaagttcg agggagacac cctcgtcaac cgtatcgagc tcaagggagt
     5461 cgacttcaag gaggacggaa acatcctcgg acacaagctc gagtacaact tcaactccca
     5521 caacatctac atcatggccg tcaagcagaa gaacggaatc aaggtaagtt taaacatgat
     5581 tttactaact aactaatctg atttaaattt tcaggtcaac ttcaagatcc gtcacaacgt
     5641 cgaggacgga tccgtccagc tcgccgacca ctaccagcag aacaccccaa tcggagacgg
     5701 accagtcctc ctcccagact cccactacct ctccacccag tccgtcctct ccaaggaccc
     5761 aaacgagaag cgtgaccaca tggtcctcct cgagttccgt accgccgccg gaatcaccct
     5821 cggaatggac gagctctaca agtgataaat gcaaaatcct ttcaagcatt cccttcttct
     5881 ctatcactct tctttctttt tgtcaaaaaa ttctctcgct aatttatttg cttttttaat
     5941 gttattattt tatgactttt tatagtcact gaaaagtttg catctgagtg aagtgaatgc
     6001 tatcaaaatg tgattctgtc tgatgtactt tcacaatctc tcttcaattc cattttgaag
     6061 tgctttaaac ccgaaaggtt gagaaaaatg cgagcgctca aatatttgta ttgtgttcgt
     6121 tgagtgaccc aacaaaaaga ggaaacttta ttgtgccgcc aagaaaaaag tctcaataac
     6181 ttcgtatagc atacattata cgaagttatg ctagcatggc ctcccaggtc gtgtacggcg
     6241 agtgggccag cacccacaca gagtgctaca acatgagcag gatcgacagc accttcgtga
     6301 gcctgctgca gctggtgtgg gccgtggtga gcggatgcca gaccatcttc atgatcagca
     6361 gggcccccaa ggtgccctgg gagagcgtgt acctgccctt cgtggagtcc atcacatacg
     6421 ccctggccag caccggcaat ggcacactgc agatgaggga cggcaggttc tttccctggt
     6481 ccaggatggc cagctggctg tgcacatgcc ccattatgct gggccagatc tccaacatgg
     6541 ctctggtgaa gtacaagagc atccctctga accccatcgc ccaggccgcc agcattatca
     6601 gggtggtgat gggcattacc gctaccatct cccccgccga gtacatgaag tggctgttct
     6661 tcttcttcgg cgctacctgc ctggtcttcg aatacagcgt ggtgttcacc atcttccagg
     6721 tgggactgta cggcttcgag tccgtgggca cccctctggc ccagaaggtg gtggtgagga
     6781 tcaagatgct gaggctgatc ttcttcatcg cctggaccat gttccccatc gtgtggctga
     6841 tttcccccac cggcgtgtgc gtgatccacg agaacgtgag cgccatcctg tatctgctcg
     6901 ccgacggcct gtgcaagaac acctacggcg tgatcctgtg gtccaccgcc tggggagtgc
     6961 tcgagggaaa gtgggacccg gcttgcctcc ctggccagga gaagcctgag gccgacgacc
     7021 ccttcggact gaaccacgag aagaatgccc cccccaacga cgaggtcaac atcagaatgt
     7081 tcggcagggc ggccgccgcg gtaccgggag gaggaaccat ggtctcaaag ggtgaagaag
     7141 ataacatggc aattattaaa gagtttatgc gtttcaaggt gcatatggag ggatctgtca
     7201 atgggcatga gtttgaaatt gaaggtgaag gagaaggccg accatatgag ggaacacaaa
     7261 ccgcaaaact aaaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
     7321 ttcaggtaac taaaggcgga ccattaccat tcgcctggga catcctctct ccacagttca
     7381 tgtatggaag taaagcttat gttaaacatc cggcagatat accagattat ttgaaacttt
     7441 cattcccgga gggttttaag tgggaacgcg taatgaattt tgaagacgga ggagttgtta
     7501 cagtgacgca agactcaagg taagtttaaa cagttcggta ctaactaacc atacatattt
     7561 aaattttcag cctccaagat ggagaattta tttataaagt caaacttcga ggaacgaatt
     7621 tcccctcgga tggatctgtt atgcagaaga agactatggg atgggaagct tcaagtgaaa
     7681 gaatgtaccc tgaagacggt gctcttaagg gagagattaa acaacgtctt aaattgaaag
     7741 atggaggaca ttacgatgct gaggtaagtt taaacatgat tttactaact aactaatctg
     7801 atttaaattt tcaggtgaag acaacttaca aagccaaaaa accagttcag ctgccaggag
     7861 cgtacaatgt taatattaaa ctggatatca cctcccacaa cgaggattac actatcgttg
     7921 agcaatatga aagagctgaa gggcggcact cgacaggtgg catggatgaa ttgtataagt
     7981 tctgctacga gaacgaggtc taagaattcc aactgagcgc cggtcgctac cattaccaac
     8041 ttgtctggtg tcaaaaataa taggggccgc tgtcatcaga gtaagtttaa actgagttct
     8101 actaactaac gagtaatatt taaattttca gcatctcgcg cccgtgcctc tgacttctaa
     8161 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     8221 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     8281 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     8341 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     8401 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     8461 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttatttcttc
     8521 gcacgtctgg gcctctcatg acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt
     8581 tttggaattt ttcaatcaag tgaaagttta tgaaattaat tttcctgctt ttgctttttg
     8641 gggtttcccc tattgtttgt caagatttcg aggacggcgt ttttcttgct aaaatcacaa
     8701 gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag gctcagtgga
     8761 aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa
     8821 tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg gccgtacggg
     8881 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     8941 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     9001 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     9061 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     9121 atcaggcggc cttaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     9181 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9241 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9301 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9361 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9421 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9481 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9541 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9601 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     9661 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     9721 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     9781 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     9841 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     9901 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     9961 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    10021 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10081 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10141 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10201 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10261 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10321 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10381 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10441 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10501 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10561 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10621 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    10681 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10741 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10801 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10861 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10921 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10981 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    11041 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    11101 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    11161 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    11221 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    11281 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    11341 cagctatgac catgattacg ccaagctgta agt
//
