LOCUS       sequence-375282-       10737 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Obtained by cloning 5 stop codons in place of the EGFP Kozak 
            sequence in pWPT-/mEGFP-1T-IRES-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10737)
  AUTHORS   Ambrosini C, Destefanis E, Kheir E, Broso F, Alessandrini F, Longhi 
            S, Battisti N, Pesce I, Dassi E, Petris G, Cereseto A, Quattrone A
  TITLE     Translational enhancement by base editing of the Kozak sequence 
            rescues haploinsufficiency.
  JOURNAL   Nucleic Acids Res. 2022 Oct 14;50(18):10756-10771. doi: 
            10.1093/nar/gkac799.
  PUBMED    36165847
REFERENCE   2  (bases 1 to 10737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-10-14- 14"; volume: "50"; issue: 
            "18"; pages: "10756-10771"
COMMENT     Sequence Label: pWPT-mCherry
FEATURES             Location/Qualifiers
     source          1..10737
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             15..725
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(54..75)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(315..334)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     662..683
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(776..1350,1351..1353,1354..1362)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(943..960)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1170..1189
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      1371..1380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1377..2087
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1522..1540)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1790..1809
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2100..2688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2153..2173)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2571..2582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2833..2866)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             2886..3066
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3091..3109)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3092..3109)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3399..3418)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3632..3651)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3751..3773
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3811..3829)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3897..4001
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4002..4070,4071..4862)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4220..4239)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5033..5621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5522..5541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5775..5792
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5862..5882)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5867..6196
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6047..6182
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7330..7395
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7525..7545
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7970..8104
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8007..8026)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8061..8080
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8272..8905
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8952..9077
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9574..9807
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9992..10036
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10303..10420
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        10477..10688
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 agtgataaga attcagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
       61 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      121 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      181 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
      241 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      301 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      361 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      421 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      481 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      541 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      601 acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc
      661 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      721 acaagttcga ataagcatgc atctagggcg gccaattccg cccctctccc ccccccccct
      781 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
      841 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
      901 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
      961 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     1021 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     1081 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     1141 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     1201 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     1261 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     1321 tggttttcct ttgaaaaaca cgatgataag cttgccacaa cccgggatcc gccaccatgg
     1381 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     1441 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     1501 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     1561 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     1621 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     1681 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     1741 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     1801 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     1861 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     1921 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     1981 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     2041 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgccgata
     2101 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2161 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2221 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2281 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2341 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2401 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2461 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     2521 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     2581 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     2641 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggcaattc
     2701 gagctcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     2761 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atgggatcaa
     2821 ttcaccatgg gaataacttc gtatagcata cattatacga agttatgctg ctttttgctt
     2881 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     2941 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     3001 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     3061 ctagcagcat ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat
     3121 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa
     3181 ttgtgtagca tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag
     3241 agtcggtttg gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat
     3301 ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt
     3361 ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga
     3421 tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt
     3481 ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc
     3541 ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca
     3601 taagggagag cgtcgaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     3661 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     3721 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     3781 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     3841 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     3901 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     3961 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     4021 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     4081 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     4141 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     4201 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     4261 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     4321 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     4381 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     4441 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     4501 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     4561 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     4621 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     4681 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     4741 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     4801 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     4861 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4921 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4981 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     5041 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     5101 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     5161 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     5221 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5281 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5341 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5401 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5461 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5521 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5581 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5641 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5701 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     5761 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     5821 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg tggaatgtgt gtcagttagg
     5881 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5941 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6001 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6061 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6121 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6181 cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg cgagatatgt ttgagaatac
     6241 cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac
     6301 tggtttttag tgcgccagat ctctataatc tcgcgcaacc tattttcccc tcgaacactt
     6361 tttaagccgt agataaacag gctgggacac ttcacatgag cgaaaaatac atcgtcacct
     6421 gggacatgtt gcagatccat gcacgtaaac tcgcaagccg actgatgcct tctgaacaat
     6481 ggaaaggcat tattgccgta agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga
     6541 actgggtatt cgtcatgtcg ataccgtttg tatttccagc tacgatcacg acaaccagcg
     6601 cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga
     6661 cctggtggat accggtggta ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt
     6721 tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt gatgactatg ttgttgatat
     6781 cccgcaagat acctggattg aacagccgtg ggatatgggc gtcgtattcg tcccgccaat
     6841 ctccggtcgc taatcttttc aacgcctggc actgccgggc gttgttcttt ttaacttcag
     6901 gcgggttaca atagtttcca gtaagtattc tggaggctgc atccatgaca caggcaaacc
     6961 tgagcgaaac cctgttcaaa ccccgcttta aacatcctga aacctcgacg ctagtccgcc
     7021 gctttaatca cggcgcacaa ccgcctgtgc agtcggccct tgatggtaaa accatccctc
     7081 actggtatcg catgattaac cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa
     7141 tcctcgacgt ccaggcacgt attgtgatga gcgatgccga acgtaccgac gatgatttat
     7201 acgatacggt gattggctac cgtggcggca actggattta tgagtgggcc ccggatcttt
     7261 gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa
     7321 agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt
     7381 gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc
     7441 tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact
     7501 gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac
     7561 tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct
     7621 tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa
     7681 aaatattctg taacctttat aagtaggcat aacagttata atcataacat actgtttttt
     7741 cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc
     7801 tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact
     7861 agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     7921 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     7981 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     8041 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     8101 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
     8161 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     8221 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
     8281 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     8341 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
     8401 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
     8461 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     8521 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
     8581 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
     8641 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
     8701 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
     8761 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     8821 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     8881 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     8941 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     9001 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     9061 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     9121 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     9181 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     9241 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     9301 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     9361 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     9421 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     9481 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     9541 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     9601 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     9661 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     9721 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     9781 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     9841 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     9901 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     9961 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    10021 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    10081 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    10141 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    10201 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    10261 atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg
    10321 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    10381 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct
    10441 cgacaagctt gatcgatggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag
    10501 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg
    10561 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag
    10621 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca
    10681 gaacacaggt gtcgtgacgc gcatccaggc ctaagcttac gctcgagtaa taataat
//
