LOCUS       sequence-375284-       10375 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Obtained by creating a deletion inside the mCherry coding sequence 
            in pWPT-/GCCACC-mEGFP-IRES-mCherry (Addgene #49235).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Ambrosini C, Destefanis E, Kheir E, Broso F, Alessandrini F, Longhi 
            S, Battisti N, Pesce I, Dassi E, Petris G, Cereseto A, Quattrone A
  TITLE     Translational enhancement by base editing of the Kozak sequence 
            rescues haploinsufficiency.
  JOURNAL   Nucleic Acids Res. 2022 Oct 14;50(18):10756-10771. doi: 
            10.1093/nar/gkac799.
  PUBMED    36165847
REFERENCE   2  (bases 1 to 10375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2022-10-14- 14"; volume: "50"; issue: 
            "18"; pages: "10756-10771"
COMMENT     Sequence Label: pWPT-mEGFP
FEATURES             Location/Qualifiers
     source          1..10375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..46
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(264..974)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(306..327)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     655..674
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     914..935
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1043..1254)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    1311..1428
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1695..1739)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1924..2157
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2654..2779
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2826..3459
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    3627..3761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3651..3670)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3705..3724
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(4186..4206)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          4336..4401
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(5535..5864)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5549..5684
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5849..5869
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(5939..5956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6110..6698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6190..6209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6869..7660,7661..7729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7492..7511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7730..7834)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7902..7920
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7958..7980)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8080..8099
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8313..8332
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        8622..8640
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8622..8639
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             8665..8845
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     protein_bind    8865..8898
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    9043..9631
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9149..9160
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9558..9578
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(9922..9941)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    join(10002..10010,10011..10013,10014..10375,1..213)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10175..10194)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gcttccttca cgacattcaa cagaccttgc attcctttgg cgagagggga aagaccccta
       61 ggaatgctcg tcaagaagac agggccaggt ttccgggccc tcacattgcc aaaagacggc
      121 aatatggtgg aaaataacat atagacaaac gcacaccggc cttattccaa gcggcttcgg
      181 ccagtaacgt tagggggggg ggagggagag gggggggggg gagaggggcg gaattggccg
      241 ccctagatgc atgcttattc gaacttgtac agctcgtcca tgccgagagt gatcccggcg
      301 gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc
      361 ttggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg
      421 gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc
      481 ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg
      541 ttgtagttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      601 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      661 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg
      721 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
      781 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
      841 aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac
      901 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
      961 tcctcgccct tgctgaattc gcccatggtg gcttagttag ttactcgagc gtaagcttag
     1021 gcctggatgc gcgtcacgac acctgtgttc tggcggcaaa cccgttgcga aaaagaacgt
     1081 tcacggcgac tactgcactt atatacggtt ctcccccacc ctcgggaaaa aggcggagcc
     1141 agtacacgac atcactttcc cagtttaccc cgcgccacct tctctaggca ccggttcaat
     1201 tgccgacccc tccccccaac ttctcgggga ctgtgggcga tgtgcgctct gcccactgac
     1261 gggcaccgga gccatcgatc aagcttgtcg aggctagtct cgtgatcgat aaaattttga
     1321 atttttgtaa tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact
     1381 attctttccc ctgcactgtc ccccccaatc cccccttttc ttttaaaagt taaccgatac
     1441 cgtcgagatc cgttcactaa tcgaatggat ctgtctctgt ctctctctcc accttcttct
     1501 tctattcctt cgggcctgtc gggtcccctc ggggttggga ggtgggtctg aaacgataat
     1561 ggtgaatatc cctgcctaac tctattcact atagaaagta cagcaaaaac tattcttaaa
     1621 cctaccaagc ctcctactat cattatgaat aattttatat accacagcca atttgttatg
     1681 ttaaaccaat tccacaaact tgcccattta tctaattcca ataattcttg ttcattcttt
     1741 tcttgctggt tttgcgattc ttcaattaag gagtgtatta agcttgtgta attgttaatt
     1801 tctctgtccc actccatcca ggtcgtgtga ttccaaatct gttccagaga tttattactc
     1861 caactagcat tccaaggcac agcagtggtg caaatgagtt ttccagagca accccaaatc
     1921 cccaggagct gttgatcctt taggtatctt tccacagcca ggattcttgc ctggagctgc
     1981 ttgatgcccc agactgtgag ttgcaacaga tgctgttgcg cctcaatagc cctcagcaaa
     2041 ttgttctgct gctgcactat accagacaat aattgtctgg cctgtaccgt cagcgtcatt
     2101 gacgctgcgc ccatagtgct tcctgctgct cccaagaacc caaggaacaa agctcctatt
     2161 cccactgctc ttttttctct ctgcaccact cttctctttg ccttggtggg tgctactcct
     2221 aatggttcaa tttttactac tttatattta tataattcac ttctccaatt gtccctcata
     2281 tctcctcctc caggtctgaa gatcagcggc cggccgcttg ctgtgcggtg gtcttacttt
     2341 tgttttgctc ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt
     2401 gatgcacaca atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg
     2461 tctgaaggga tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca
     2521 ggccaggatt aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt
     2581 tatatttttt ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc
     2641 cccgcttaat actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa
     2701 tttttggcgt actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc
     2761 gagtcctgcg tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc
     2821 gccactgcta gagattttcc acactgacta aaagggtctg agggatctct agttaccaga
     2881 gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt
     2941 aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag
     3001 agacccagta caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc
     3061 cagtcccgcc caggccacgc ctccctggaa agtccccagc ggaaagtccc ttgtagcaag
     3121 ctcgatatca gcagttcttg aagtactccg gatgcagctc tcgggccacg tgatgaaatg
     3181 ctaggcggct gtcaaacctc cactctaaca cttctctctc cgggtcatcc atcccatgca
     3241 ggctcacagg gtgtaacaag ctggtgttct ctcctttatt ggcctcttct accttatctg
     3301 gctcaactgg tactagcttg tagcaccatc caaaggtcag tggatatctg acccctggcc
     3361 ctggtgtgta gttctgctaa tcagggaagt agccttgtgt gtggtagatc cacagatcaa
     3421 ggatatcttg tcttctttgg gagtgaatta gcccttccaa ctactaagtt tgtagtacat
     3481 atttaacaaa tacaatttct ttaaaatgaa aataattcag aggaatcaca ggtttagagt
     3541 aaatgaaacc acaggtaatt ggcagtggta atagggtatg gggtgggaag tttgggatga
     3601 ttttggttag cttgagttat ccagttgatc cagacatgat aagatacatt gatgagtttg
     3661 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     3721 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3781 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     3841 acaaatgtgg tatggctgat tatgatctct agtcaaggca ctatacatca aatattcctt
     3901 attaacccct ttacaaatta aaaagctaaa ggtacacaat ttttgagcat agttattaat
     3961 agcagacact ctatgcctgt gtggagtaag aaaaaacagt atgttatgat tataactgtt
     4021 atgcctactt ataaaggtta cagaatattt ttccataatt ttcttgtata gcagtgcagc
     4081 tttttccttt gtggtgtaaa tagcaaagca agcaagagtt ctattactaa acacagcatg
     4141 actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg tcttctacct ttctcttctt
     4201 ttttggagga gtagaatgtt gagagtcagc agtagcctca tcatcactag atggcatttc
     4261 ttctgagcaa aacaggtttt cctcattaaa ggcattccac cactgctccc attcatcagt
     4321 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac
     4381 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta
     4441 tgtcacacca cagaagtaag gttccttcac aaagatccgg ggcccactca taaatccagt
     4501 tgccgccacg gtagccaatc accgtatcgt ataaatcatc gtcggtacgt tcggcatcgc
     4561 tcatcacaat acgtgcctgg acgtcgagga tttcgcgtgg gtcaatgccg cgccagatcc
     4621 acatcagacg gttaatcatg cgataccagt gagggatggt tttaccatca agggccgact
     4681 gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt tcaggatgtt
     4741 taaagcgggg tttgaacagg gtttcgctca ggtttgcctg tgtcatggat gcagcctcca
     4801 gaatacttac tggaaactat tgtaacccgc ctgaagttaa aaagaacaac gcccggcagt
     4861 gccaggcgtt gaaaagatta gcgaccggag attggcggga cgaatacgac gcccatatcc
     4921 cacggctgtt caatccaggt atcttgcggg atatcaacaa catagtcatc aaccagcgga
     4981 cgaccagccg gttttgcgaa gatggtgaca aagtgcgctt ttggatacat ttcacgaatc
     5041 gcaaccgcag taccaccggt atccaccagg tcatcaataa cgatgaagcc ttcgccatcg
     5101 ccttctgcgc gtttcagcac tttaagctcg cgctggttgt cgtgatcgta gctggaaata
     5161 caaacggtat cgacatgacg aatacccagt tcacgcgcca gtaacgcacc cggtacagac
     5221 cgccacggct tacggcaata atgcctttcc attgttcaga aggcatcagt cggcttgcga
     5281 gtttacgtgc atggatctgc aacatgtccc aggtgacgat gtatttttcg ctcatgtgaa
     5341 gtgtcccagc ctgtttatct acggcttaaa aagtgttcga ggggaaaata ggttgcgcga
     5401 gattatagag atctggcgca ctaaaaacca gtatttcaca tgagtccgcg tctttttacg
     5461 cactgcctct ccctgacgcg ggataaagtg gtattctcaa acatatctcg caagcctgtc
     5521 ttgtgtccaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
     5581 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcca
     5641 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
     5701 gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
     5761 cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg catacttctg
     5821 cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca cagctgcatt
     5881 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5941 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6001 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6061 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6301 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6541 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6661 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6721 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6781 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6841 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6901 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6961 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7021 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7081 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7141 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7201 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7261 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7321 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7381 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7441 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     7501 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     7561 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     7621 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     7681 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7741 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7801 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7861 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     7921 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     7981 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     8041 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     8101 tgagagtgca ccattcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     8161 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     8221 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     8281 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     8341 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta
     8401 gcgatgaccc tgctgattgg ttcgctgacc atttccgggt gcgggacggc gttaccagaa
     8461 actcagaagg ttcgtccaac caaaccgact ctgacggcag tttacgagag agatgatagg
     8521 gtctgcttca gtaagccaga tgctacacaa ttaggcttgt acatattgtc gttagaacgc
     8581 ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg acactataga
     8641 atacacggaa ttaattctag atgctgctag agattttcca cactgactaa aagggtctga
     8701 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     8761 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     8821 agatctggtc taaccagaga gacccagtac aagcaaaaag cagcataact tcgtataatg
     8881 tatgctatac gaagttattc ccatggtgaa ttgatcccat cttgtcttcg ttgggagtga
     8941 attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac agctgccttg
     9001 taagtcattg gtcttaaagg taccgagctc gaattgcccg atgcggggag gcggcccaaa
     9061 gggagatccg actcgtctga gggcgaaggc gaagacgcgg aagaggccgc agagccggca
     9121 gcaggccgcg ggaaggaagg tccgctggat tgagggccga agggacgtag cagaaggacg
     9181 tcccgcgcag aatccaggtg gcaacacagg cgagcagcca tggaaaggac gtcagcttcc
     9241 ccgacaacac cacggaattg tcagtgccca acagccgagc ccctgtccag cagcgggcaa
     9301 ggcaggcggc gatgagttcc gccgtggcaa tagggagggg gaaagcgaaa gtcccggaaa
     9361 ggagctgaca ggtggtggca atgccccaac cagtgggggt tgcgtcagca aacacagtgc
     9421 acaccacgcc acgttgcctg acaacgggcc acaactcctc ataaagagac agcaaccagg
     9481 atttatacaa ggaggagaaa atgaaagcca tacgggaagc aatagcatga tacaaaggca
     9541 ttaaagcagc gtatccacat agcgtaaaag gagcaacata gttaagaata ccagtcaatc
     9601 tttcacaaat tttgtaatcc agaggttgat tatcggcggc cgcttacttg tacagctcgt
     9661 ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta ctgttccacg atggtgtagt
     9721 cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt gtaggcgccg ggcagctgca
     9781 cgggcttctt ggccttgtag gtggtcttga cctcagcgtc gtagtggccg ccgtccttca
     9841 gcttcagcct ctgcttgatc tcgcccttca gggcgccgtc ctcggggtac atccgctcgg
     9901 aggaggcctc ccagcccatg gtcttcttct gcattacggg gccgtcggag gggaagttgg
     9961 tgccgcgcag cttcaccttg tagatgaact cgccgtcccc gggttgtggc aagcttatca
    10021 tcgtgttttt caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa
    10081 cctcgactaa acacatgtaa agcatgtgca ccgaggcccc agatcagatc ccatacaatg
    10141 gggtaccttc tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac
    10201 tctttccaca actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt
    10261 atcttataca cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg
    10321 cctgcaaagg gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaact
//
