LOCUS       sequence-375298-       15629 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-BirA 
            3xFLAG-CSE1L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15629)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 15629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK BirA* 3xFLAG-CSE1L zeo
FEATURES             Location/Qualifiers
     source          1..15629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2991..3341,3342..3344,3345..3953)
                     /codon_start=1
                     /product="promiscuous R118G mutant of the E. coli biotin 
                     protein ligase (Cronan, 2005)"
                     /label=BirA(R118G)
                     /note="also known as BirA* or BioID"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRD
                     WGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGELKSG
                     DACIAEYQQAGRGGRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEVLRK
                     LGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESVVNQG
                     WITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIG
                     DKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     CDS             3963..4028
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(6980..7004)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7054..8265
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8102..8121
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(8324..8941,8942..8947,8948..8983,8984..8986,8987..9022,
                     9023..9025,9026..9061,9062..9070)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    9108..9681
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9275..9292)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9502..9521
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        9703..9750
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9769..10143
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    10317..10905
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10370..10390)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10788..10799)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10908..10924)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10909..10925)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11434..11614
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11640..11657)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11646..11870
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11916..12344
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12003..12022)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12213..12234
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12353..12373)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12358..12687
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12538..12673
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12600..12619
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12735..12782
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12801..13175
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13305..13426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13342..13361)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13396..13415
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13475..13491)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13475..13491)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13488..13510)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13499..13515
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13523..13529,13530..13547,13548..13553))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13568..13589
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13706..13723)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13877..14465)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13957..13976)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14636..15427,15428..15496))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     15259..15278
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15497..15601)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga atgaaggaca
     3001 acaccgtgcc cctgaagctg atcgccctgc tggccaacgg cgagttccac tctggcgagc
     3061 agctgggaga gaccctggga atgagcagag ccgccatcaa caagcacatc cagacactga
     3121 gagactgggg agtggacgtg ttcaccgtgc ctggcaaggg ctacagcctg cctgagccta
     3181 tccagctgct gaacgccaag cagatcctgg gacagctgga tggcggaagc gtggccgtgc
     3241 tgcctgtgat cgactccacc aatcagtacc tgctggacag aatcggagag ctgaagtccg
     3301 gcgacgcctg catcgccgag taccagcagg ctggcagagg aggcagagga cggaagtggt
     3361 tcagcccatt cggagccaac ctgtacctgt ccatgttctg gagactggag cagggacctg
     3421 ctgctgccat cggactgagt ctggtgatcg gaatcgtgat ggccgaggtg ctgagaaagc
     3481 tgggagccga caaggtgaga gtgaagtggc ctaatgacct gtacctccag gaccgcaagc
     3541 tggctggcat cctggtggag ctgacaggca agacaggcga tgccgctcag atcgtgatcg
     3601 gagccggaat caacatggcc atgagaagag tggaggagag cgtggtgaac cagggctgga
     3661 tcaccctgca ggaggctggc atcaacctgg accggaacac cctggccgcc atgctgatca
     3721 gagagctgag agccgctctg gagctgttcg agcaggaggg actggctcct tacctgagca
     3781 gatgggagaa gctggacaac ttcatcaaca gacctgtgaa gctgatcatc ggcgacaagg
     3841 aaatcttcgg catctccaga ggaatcgaca agcagggagc tctgctgctg gagcaggacg
     3901 gaatcatcaa gccctggatg ggcggagaaa tctccctgag aagcgcagag aagactagaa
     3961 tggactacaa agaccatgac ggtgattata aagatcatga catcgattac aaggatgacg
     4021 atgacaagat ggaactcagc gatgcaaatc tgcaaacact aacagaatat ttaaagaaaa
     4081 cacttgatcc tgatcctgcc atccgacgtc cagctgagaa atttcttgaa tctgttgaag
     4141 gaaatcagaa ttatccactg ttgcttttga cattactgga gaagtcccag gataatgtta
     4201 tcaaagtatg tgcttcagta acattcaaaa actatattaa aaggaactgg agaattgttg
     4261 aagatgaacc aaacaaaatt tgtgaagccg atcgagtggc cattaaagcc aacatagtgc
     4321 acttgatgct tagcagccca gagcaaattc agaagcagtt aagtgatgca attagcatta
     4381 ttggcagaga agattttcca cagaaatggc ctgacttgct gacagaaatg gtgaatcgct
     4441 ttcagagtgg agatttccat gttattaatg gagtcctccg tacagcacat tcattattta
     4501 aaagataccg tcatgaattt aagtcaaacg agttatggac tgaaattaag cttgttctgg
     4561 atgcctttgc tttgcctttg actaatcttt ttaaggccac tattgaactc tgcagtaccc
     4621 atgcaaatga tgcctctgcc ctgaggattc tgttttcttc cctgatcctg atctcaaaat
     4681 tgttctatag tttaaacttt caggatctcc ctgaattttt tgaagataat atggaaactt
     4741 ggatgaataa ttttcatact ctcttaacat tggataataa gcttttacaa actgatgatg
     4801 aagaggaagc cggcttattg gagctcttaa aatcccagat ttgtgataat gccgcactct
     4861 atgcacaaaa gtacgatgaa gaattccagc gatacctgcc tcgttttgtt acagccatct
     4921 ggaatttact agttacaacg ggtcaagagg ttaaatatga tttgttggta agtaatgcaa
     4981 ttcaatttct ggcttcagtt tgtgagagac ctcattataa gaatctattt gaggaccaga
     5041 acacgctgac aagtatctgt gaaaaggtta ttgtgcctaa catggaattt agagctgctg
     5101 atgaagaagc atttgaagat aattctgagg agtacataag gagagatttg gaaggatctg
     5161 atattgatac tagacgcagg gctgcttgtg atctggtacg aggattatgc aagttttttg
     5221 agggacctgt gacaggaatc ttctctggtt atgttaattc catgctgcag gaatacgcaa
     5281 aaaatccatc tgtcaactgg aaacacaaag atgcagccat ctacctagtg acatctttgg
     5341 catcaaaagc ccaaacacag aagcatggaa ttacacaagc aaatgaactt gtaaacctaa
     5401 ctgagttctt tgtgaatcac atcctccctg atttaaaatc agctaatgtg aatgaatttc
     5461 ctgtccttaa agctgacggt atcaaatata ttatgatttt tagaaatcaa gtgccaaaag
     5521 aacatctttt agtctcgatt cctctcttga ttaatcatct tcaagctgaa agtattgttg
     5581 ttcatactta cgcagctcat gctcttgaac ggctctttac tatgcgaggg cctaacaatg
     5641 ccactctctt tacagctgca gaaatcgcac cgtttgttga gattctgcta acaaaccttt
     5701 tcaaagctct cacacttcct ggctcttcag aaaatgaata tattatgaaa gctatcatga
     5761 gaagtttttc tctcctacaa gaagccataa tcccctacat ccctactctc atcactcagc
     5821 ttacacagaa gctattagct gttagtaaga acccaagcaa acctcacttt aatcactaca
     5881 tgtttgaagc aatatgttta tccataagaa taacttgcaa agctaaccct gctgctgttg
     5941 taaattttga ggaggctttg tttttggtgt ttactgaaat cttacaaaat gatgtgcaag
     6001 aatttattcc atacgtcttt caagtgatgt ctttgcttct ggaaacacac aaaaatgaca
     6061 tcccgtcttc ctatatggcc ttatttcctc atctccttca gccagtgctt tgggaaagaa
     6121 caggaaatat tcctgctcta gtgaggcttc ttcaagcatt cttagaacgc ggttcaaaca
     6181 caatagcaag tgctgcagct gacaaaattc ctgggttact aggtgtcttt cagaagctga
     6241 ttgcatccaa agcaaatgac caccaaggtt tttatcttct aaacagtata atagagcaca
     6301 tgcctcctga atcagttgac caatatagga aacaaatctt cattctgcta ttccagagac
     6361 ttcagaattc caaaacaacc aagtttatca agagtttttt agtctttatt aatttgtatt
     6421 gcataaaata tggggcacta gcactacaag aaatatttga tggtatacaa ccaaaaatgt
     6481 ttggaatggt tttggaaaaa attattattc ctgaaattca gaaggtatct ggaaatgtag
     6541 agaaaaagat ctgtgcggtt ggcataacca aattactaac agaatgtccc ccaatgatgg
     6601 acactgagta taccaaactg tggactccat tattacagtc tttgattggt ctttttgagt
     6661 tacccgaaga tgataccatt cctgatgagg aacattttat tgacatagaa gatacaccag
     6721 gatatcagac tgccttctca cagttggcat ttgctgggaa aaaagagcat gatcctgtag
     6781 gtcaaatggt gaataacccc aaaattcacc tggcacagtc acttcacaag ttgtctaccg
     6841 cctgtccagg aagggttcca tcaatggtga gcaccagcct gaatgcagaa gcgctccagt
     6901 atctccaagg gtaccttcag gcagccagtg tgacactgct ttaacaattg gaagactaat
     6961 gcgttttcga gatatctaga cccagctttc ttgtacaaag tggttgataa catctcgagc
     7021 cataaagatg gttaattaac ccacccaaga tctggcctcc gcgccgggtt ttggcgcctc
     7081 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
     7141 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
     7201 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
     7261 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
     7321 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     7381 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
     7441 atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc
     7501 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc
     7561 aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc
     7621 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg
     7681 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg
     7741 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga
     7801 actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc
     7861 cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat
     7921 aatgcagggt ggggccacct gccggtaggt gtgcggtagg cttttctccg tcgcaggacg
     7981 cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg
     8041 gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag
     8101 ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt
     8161 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt
     8221 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacgaagc ttggtaccga
     8281 gctcggatct ccaccccgta ccggtcctgc agtcgaattc accatgtcta gactggacaa
     8341 gagcaaagtc ataaacggag ctctggaatt actcaatggt gtcggtatcg aaggcctgac
     8401 gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa
     8461 gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca
     8521 cttctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata
     8581 ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac
     8641 agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc
     8701 cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt
     8761 ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat
     8821 gcccccactt ctgagacaag caattgagct gttcgaccgg cagggagccg aacctgcctt
     8881 ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg
     8941 cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga
     9001 cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct
     9061 ccccgggtaa ctaagtaagg atccgcggcc gcactagagg aattccgccc ctctcccccc
     9121 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     9181 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     9241 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     9301 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     9361 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     9421 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     9481 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     9541 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     9601 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     9661 ctttgaaaaa cacgatgata agcttaccgg tacgcgtgat gtgttgacaa ttaatcatcg
     9721 gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat ggccaagttg
     9781 accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga gttctggacc
     9841 gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt ggtccgggac
     9901 gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa caccctggcc
     9961 tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg
    10021 aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc gtgggggcgg
    10081 gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga ggagcaggac
    10141 tgacacatct gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacatt
    10201 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    10261 aatgaatgca attgttgttg tttagtccct cccaattcga tatcaagctt atcgataatc
    10321 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
    10381 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
    10441 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
    10501 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
    10561 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
    10621 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
    10681 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
    10741 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
    10801 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
    10861 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
    10921 ctcgatcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
    10981 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
    11041 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    11101 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
    11161 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
    11221 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
    11281 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
    11341 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
    11401 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
    11461 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    11521 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    11581 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
    11641 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
    11701 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
    11761 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
    11821 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
    11881 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
    11941 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    12001 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    12061 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    12121 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    12181 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    12241 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    12301 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
    12361 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    12421 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
    12481 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
    12541 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    12601 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
    12661 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
    12721 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
    12781 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
    12841 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
    12901 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
    12961 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
    13021 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
    13081 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
    13141 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
    13201 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
    13261 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
    13321 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    13381 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
    13441 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    13501 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    13561 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    13621 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    13681 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    13741 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    13801 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    13861 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    13921 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    13981 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    14041 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    14101 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    14161 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    14221 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    14281 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    14341 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    14401 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    14461 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    14521 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    14581 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    14641 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    14701 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    14761 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    14821 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    14881 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    14941 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    15001 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    15061 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    15121 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    15181 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    15241 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    15301 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    15361 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    15421 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    15481 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    15541 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    15601 gcacatttcc ccgaaaagtg ccacctgac
//
