LOCUS       sequence-375801-       14660 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Lentiviral expression vector for an inducible 3xFLAG-CSE1L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14660)
  AUTHORS   Wang L, Paudel BB, McKnight RA, Janes KA
  TITLE     Nucleocytoplasmic transport of active HER2 causes fractional escape 
            from the DCIS-like state.
  JOURNAL   Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x.
  PUBMED    37055441
REFERENCE   2  (bases 1 to 14660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-04-13"; volume: "14(1):2110. doi"; pages: " 
            10.1038/s41467-023-37914-x"
COMMENT     Sequence Label: pSLIK 3xFLAG-CSE1L zeo
FEATURES             Location/Qualifiers
     source          1..14660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2614..2638
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2670..2984
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2678..2696
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2714..2732
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2749..2767
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2856..2874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2957..2981
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2994..3059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(6011..6035)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        6085..7296
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     7133..7152
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(7355..7972,7973..7978,7979..8014,8015..8017,8018..8053,
                     8054..8056,8057..8092,8093..8101)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8139..8712
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8306..8323)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8533..8552
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     promoter        8734..8781
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8800..9174
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    9348..9936
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9401..9421)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9819..9830)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9939..9955)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9940..9956)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10465..10645
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10671..10688)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10677..10901
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10947..11375
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11034..11053)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11244..11265
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11384..11404)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11389..11718
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11569..11704
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11631..11650
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11766..11813
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11832..12206
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12336..12457
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12373..12392)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12427..12446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12506..12522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12506..12522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12519..12541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12530..12546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12554..12560,12561..12578,12579..12584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12599..12620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12737..12754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12908..13496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12988..13007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13667..14458,14459..14527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14290..14309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14528..14632)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagacagtta tcaacaagtt tgtacaaaaa agcaggcttt
     2641 aaaggaacca attcagtcga ctggatcccg agtttactcc ctatcagtga tagagaacgt
     2701 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2761 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2881 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2941 aggcctatat aagcagagct cgtttagtga accgtcagat cgccactaga atggactaca
     3001 aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac gatgacaaga
     3061 tggaactcag cgatgcaaat ctgcaaacac taacagaata tttaaagaaa acacttgatc
     3121 ctgatcctgc catccgacgt ccagctgaga aatttcttga atctgttgaa ggaaatcaga
     3181 attatccact gttgcttttg acattactgg agaagtccca ggataatgtt atcaaagtat
     3241 gtgcttcagt aacattcaaa aactatatta aaaggaactg gagaattgtt gaagatgaac
     3301 caaacaaaat ttgtgaagcc gatcgagtgg ccattaaagc caacatagtg cacttgatgc
     3361 ttagcagccc agagcaaatt cagaagcagt taagtgatgc aattagcatt attggcagag
     3421 aagattttcc acagaaatgg cctgacttgc tgacagaaat ggtgaatcgc tttcagagtg
     3481 gagatttcca tgttattaat ggagtcctcc gtacagcaca ttcattattt aaaagatacc
     3541 gtcatgaatt taagtcaaac gagttatgga ctgaaattaa gcttgttctg gatgcctttg
     3601 ctttgccttt gactaatctt tttaaggcca ctattgaact ctgcagtacc catgcaaatg
     3661 atgcctctgc cctgaggatt ctgttttctt ccctgatcct gatctcaaaa ttgttctata
     3721 gtttaaactt tcaggatctc cctgaatttt ttgaagataa tatggaaact tggatgaata
     3781 attttcatac tctcttaaca ttggataata agcttttaca aactgatgat gaagaggaag
     3841 ccggcttatt ggagctctta aaatcccaga tttgtgataa tgccgcactc tatgcacaaa
     3901 agtacgatga agaattccag cgatacctgc ctcgttttgt tacagccatc tggaatttac
     3961 tagttacaac gggtcaagag gttaaatatg atttgttggt aagtaatgca attcaatttc
     4021 tggcttcagt ttgtgagaga cctcattata agaatctatt tgaggaccag aacacgctga
     4081 caagtatctg tgaaaaggtt attgtgccta acatggaatt tagagctgct gatgaagaag
     4141 catttgaaga taattctgag gagtacataa ggagagattt ggaaggatct gatattgata
     4201 ctagacgcag ggctgcttgt gatctggtac gaggattatg caagtttttt gagggacctg
     4261 tgacaggaat cttctctggt tatgttaatt ccatgctgca ggaatacgca aaaaatccat
     4321 ctgtcaactg gaaacacaaa gatgcagcca tctacctagt gacatctttg gcatcaaaag
     4381 cccaaacaca gaagcatgga attacacaag caaatgaact tgtaaaccta actgagttct
     4441 ttgtgaatca catcctccct gatttaaaat cagctaatgt gaatgaattt cctgtcctta
     4501 aagctgacgg tatcaaatat attatgattt ttagaaatca agtgccaaaa gaacatcttt
     4561 tagtctcgat tcctctcttg attaatcatc ttcaagctga aagtattgtt gttcatactt
     4621 acgcagctca tgctcttgaa cggctcttta ctatgcgagg gcctaacaat gccactctct
     4681 ttacagctgc agaaatcgca ccgtttgttg agattctgct aacaaacctt ttcaaagctc
     4741 tcacacttcc tggctcttca gaaaatgaat atattatgaa agctatcatg agaagttttt
     4801 ctctcctaca agaagccata atcccctaca tccctactct catcactcag cttacacaga
     4861 agctattagc tgttagtaag aacccaagca aacctcactt taatcactac atgtttgaag
     4921 caatatgttt atccataaga ataacttgca aagctaaccc tgctgctgtt gtaaattttg
     4981 aggaggcttt gtttttggtg tttactgaaa tcttacaaaa tgatgtgcaa gaatttattc
     5041 catacgtctt tcaagtgatg tctttgcttc tggaaacaca caaaaatgac atcccgtctt
     5101 cctatatggc cttatttcct catctccttc agccagtgct ttgggaaaga acaggaaata
     5161 ttcctgctct agtgaggctt cttcaagcat tcttagaacg cggttcaaac acaatagcaa
     5221 gtgctgcagc tgacaaaatt cctgggttac taggtgtctt tcagaagctg attgcatcca
     5281 aagcaaatga ccaccaaggt ttttatcttc taaacagtat aatagagcac atgcctcctg
     5341 aatcagttga ccaatatagg aaacaaatct tcattctgct attccagaga cttcagaatt
     5401 ccaaaacaac caagtttatc aagagttttt tagtctttat taatttgtat tgcataaaat
     5461 atggggcact agcactacaa gaaatatttg atggtataca accaaaaatg tttggaatgg
     5521 ttttggaaaa aattattatt cctgaaattc agaaggtatc tggaaatgta gagaaaaaga
     5581 tctgtgcggt tggcataacc aaattactaa cagaatgtcc cccaatgatg gacactgagt
     5641 ataccaaact gtggactcca ttattacagt ctttgattgg tctttttgag ttacccgaag
     5701 atgataccat tcctgatgag gaacatttta ttgacataga agatacacca ggatatcaga
     5761 ctgccttctc acagttggca tttgctggga aaaaagagca tgatcctgta ggtcaaatgg
     5821 tgaataaccc caaaattcac ctggcacagt cacttcacaa gttgtctacc gcctgtccag
     5881 gaagggttcc atcaatggtg agcaccagcc tgaatgcaga agcgctccag tatctccaag
     5941 ggtaccttca ggcagccagt gtgacactgc tttaacaatt ggaagactaa tgcgttttcg
     6001 agatatctag acccagcttt cttgtacaaa gtggttgata acatctcgag ccataaagat
     6061 ggttaattaa cccacccaag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg
     6121 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     6181 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     6241 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     6301 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     6361 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     6421 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     6481 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     6541 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     6601 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     6661 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     6721 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     6781 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     6841 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     6901 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     6961 tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac gcagggttcg
     7021 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     7081 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     7141 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     7201 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     7261 gctaaattct ggccgttttt ggcttttttg ttagacgaag cttggtaccg agctcggatc
     7321 tccaccccgt accggtcctg cagtcgaatt caccatgtct agactggaca agagcaaagt
     7381 cataaacgga gctctggaat tactcaatgg tgtcggtatc gaaggcctga cgacaaggaa
     7441 actcgctcaa aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg
     7501 ggccctgctc gatgccctgc caatcgagat gctggacagg catcataccc acttctgccc
     7561 cctggaaggc gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc
     7621 tctcctctca catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca
     7681 gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa
     7741 cgcactgtac gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca
     7801 ggagcatcaa gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact
     7861 tctgagacaa gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg
     7921 cctggaacta atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac
     7981 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     8041 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta
     8101 actaagtaag gatccgcggc cgcactagag gaattccgcc cctctcccyc ccccccccct
     8161 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     8221 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     8281 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     8341 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     8401 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     8461 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     8521 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     8581 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     8641 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     8701 acacgatgat aagcttaccg gtacgcgtga tgtgttgaca attaatcatc ggcatagtat
     8761 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     8821 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     8881 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     8941 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     9001 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     9061 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     9121 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacatc
     9181 tgtacaagta aagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt
     9241 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     9301 aattgttgtt gtttagtccc tcccaattcg atatcaagct tatcgataat caacctctgg
     9361 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     9421 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     9481 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     9541 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     9601 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     9661 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     9721 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     9781 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     9841 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     9901 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgatcga
     9961 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
    10021 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
    10081 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    10141 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
    10201 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
    10261 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
    10321 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
    10381 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
    10441 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
    10501 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10561 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10621 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
    10681 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
    10741 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
    10801 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
    10861 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
    10921 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
    10981 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    11041 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    11101 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    11161 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    11221 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    11281 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    11341 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
    11401 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
    11461 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
    11521 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
    11581 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
    11641 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
    11701 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
    11761 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
    11821 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
    11881 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
    11941 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
    12001 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
    12061 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
    12121 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
    12181 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
    12241 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
    12301 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
    12361 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    12421 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
    12481 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    12541 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    12601 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    12661 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    12721 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    12781 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    12841 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    12901 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    12961 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    13021 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    13081 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    13141 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    13201 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    13261 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    13321 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    13381 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    13441 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    13501 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    13561 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    13621 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    13681 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    13741 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    13801 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    13861 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    13921 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    13981 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    14041 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    14101 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    14161 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    14221 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    14281 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    14341 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    14401 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    14461 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    14521 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    14581 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    14641 cccgaaaagt gccacctgac
//
