LOCUS       sequence-37592-e        3498 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ferguson AA, Cai L, Kashyap L, Fisher AL
  TITLE     Improved vectors for selection of transgenic Caenorhabditis elegans.
  JOURNAL   Methods Mol Biol. 2013;940:87-102. doi: 10.1007/978-1-62703-110-3_8.
  PUBMED    23104336
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2013"; volume: "940:87-102. doi"; pages: " 
            10.1007/978-1-62703-110-3_8"
COMMENT     Sequence Label: punc-119cbr
FEATURES             Location/Qualifiers
     source          1..3498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2052..2063
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2221..2237)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2221..2237)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2329..2348)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2340..3155
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2340..3155
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2413..2432)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ggatcccccg gggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
       61 aactcgaggt cgactagccc gggcctagtt ctagacattc tctaatgaaa aaatctttca
      121 gttgaaattg aaaatgagtt aaagttggag tttttattga aaacagattt ccgtgtgatt
      181 agtgttttta gcgagtgtga caggacagcg aaaaaatata gaaacaaggg gggaactgaa
      241 aagcttagga atgcattgaa catgagaagg ggaaggggaa ggaacaaact agacaggaat
      301 tattggaatt taatcacatt tggagttttt tttctattcg acagaataat tatccagaac
      361 atttttgtat taaatattta tgcatcatat gagtagtcgg ctttgttgtg catgacgagt
      421 ttgttatcga cgaaatagaa gctgtcagaa cgagtctcgt ttggattgtt gatcatgtcg
      481 tccactgaaa aagagattag tcttttgaat tgtacttttt agaataatga ctcactgagt
      541 tgttgagaga gttgagggaa ctcatagata tgttcacagt tgtttcgtga attcggaata
      601 cagaatccga attcaaagtc aaaacacttc agaaggcgat ctttgaagaa gtgacgttcg
      661 atcattcgga aatgatggat tgggatgtct cctactttga attccacagt tgctccgacc
      721 gttttgagtt tcaaaaagtt tggagcaaat ctataacgca cgtatcttgc cgactcctgt
      781 ggcgactcat cattctcctg atcgttctcc ggtttggcga tctcgaaaag cacttgctca
      841 gtgtccagat cacggatttg gaacttggtg aactcgatgt tatagatgtt cgcagatggg
      901 gagcataaga atcctaaatt tatgttttaa actgaaatcc aaagggagca agataccttg
      961 agtgattccc ggaagtgcta aaacgtcgtt cggagtgatt tgagctttct tcgcaagctc
     1021 cgattccgtt gtgattcctt gttcggtgct tggtggtggc cgtggcatct ggaaatatgg
     1081 aaaagttcaa caaaaagaaa agagaaaaga atgaaatcgg atatcaagag ttagttgagc
     1141 ggtttctcta gttttctgag tctcacctgc gacgggaagg tcgccgagcc gggtggaatc
     1201 gatcgttgtt gctcggcttt catatcggtt tggttggaag cggctgaaaa cggaaagaag
     1261 tggaagaagg aaaagagtgt ggtgtgacag gaaaatggta attagagggt gccaaataac
     1321 cagctatatt ttgttttttt ttgaaaacat ttttaaaaag aaaaatacga taatgatatc
     1381 agatggattt ccggaaaact ggtatgaaaa atttcaacct ttttgagtac atgtaatcaa
     1441 aatacacttt gtaaattatc atttttattg aaactccacc atttttctat ttataacgct
     1501 aataatttga aaaagaaacc tgttgcgaac cgcggggtga atcccaaaaa cgaatgcgtt
     1561 ttggtggagt gattgattcg aatcgaagaa gaaaaagaag aagacgtgga atagagagct
     1621 cactcttaac cgagcagcac acaccgacag aaaaaaaaat gaaatgaatg agggtcttct
     1681 tcttcttctt cttcgaatga ttgacagaaa tgggaaaaag aggaagattg agaagggaaa
     1741 aaggaaggag aaaagaagca gaagaagacg tcagagagga gaggaacgag cggaaaagca
     1801 gcgggcgcaa gtcatagaag tagcagagct ggggagaaga agacactatc caagaaagga
     1861 atgacgagag agtatgcaaa ggggtatagg gtgcagacag aataggaaca gaataacaga
     1921 tgatgagcca agaagagttg aaaagggcga tgaatttgtc atgtaactta atttgggtca
     1981 atttgagcat gatgaattga aatcatccct tgttgggagt taataaccgg tttgttatca
     2041 gaaaccctgt aatagaaggg cgccctaact ttgagccaat tcatcccggt ttctgtcaaa
     2101 tatatcaaaa agtggtcaac tgacaaattg tttttgatat tataataaac attttatccg
     2161 ttaacaattt tcgaatactt tttacaagga cttggataaa ttggctcaaa gctgcagccc
     2221 gatccactag ttctagaatt gtgtctcaaa atctctgatg ttacattgca caagataaaa
     2281 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta
     2341 tgagccatat tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacatggatg
     2401 ctgatttata tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct
     2461 atcgattgta tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg
     2521 ttgccaatga tgttacagat gagatggtca gactaaactg gctgacggaa tttatgcctc
     2581 ttccgaccat caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga
     2641 tccccggaaa aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg
     2701 ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt
     2761 ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg
     2821 ttgatgcgag tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag
     2881 aaatgcataa acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac
     2941 ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg
     3001 gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc
     3061 cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat
     3121 tgcagtttca tttgatgctc gatgagtttt tctaatctag agatcgctga gataggtgcc
     3181 tcactgatta agcattggta actgtcagac ccccggggga tccgaagatc agcagttcaa
     3241 cctgttgata gtacgtacta agctctcatg tttcacgtac taagctctca tgtttaacgt
     3301 actaagctct catgtttaac gaactaaacc ctcatggcta acgtactaag ctctcatggc
     3361 taacgtacta agctctcatg gctaacgtac taagctctca tgtttgaaca ataaaattaa
     3421 tataaatcag caacttaaat agcctctaag gttttaagtt ttataagaaa aaaaagaata
     3481 tataaggctt ttaaagct
//
