LOCUS       sequence-375964-        6339 bp    DNA     circular SYN 14-APR-2025
DEFINITION  srsx-3 fluorescent neural reporter driving nuclear CYOFP expression 
            (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY37
FEATURES             Location/Qualifiers
     source          1..6339
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1698..1718
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1740..1760
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3930..3952)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4052..4071
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4149..4167)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4235..4339
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4340..4408,4409..5200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4558..4577)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5371..5959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5860..5879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6113..6130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6247..6268
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6283..6288,6289..6306,6307..6313)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6321..6337
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(6326..6339,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagt ggaagtgaag
      181 catcagaaaa ttaataacta taataataat aataaaaaag atcccgagta ataatcgtta
      241 actgtattga aaaaaagcag gaagacatga acgggcttac actcactggt ataaaaactg
      301 aataaattgt tgacgctttt tgtatgaacc ttgtttatgt gatgatgcaa aaaatctgat
      361 acgataacaa ttttatatta acaaattaat agaaacatgc taatcgtgtt tttttcaacc
      421 gctcttgaaa aattgaatag aaaatttgaa ctgaatgctt ggatttttta attcacaaaa
      481 tgtttagagc ttatatacta ttggaatact gtagactaag cctaaaaaac tagtctcata
      541 tcttctatta gagtattacg tacttaatga cgagcagtac tgcagaaatt ttaatgaatt
      601 catgatatat ttttgggtaa aagtggattg aaaagttgac caattaccga gtactatata
      661 ttttctttta tgttacccaa actgatcccc caaacttctc caacaactta tcaggtcgag
      721 aaagctattg catattctga tttcacgtca gctcctaaaa aacctaacgc acattttata
      781 tagggaatta caagtttcta gttcaaataa tcatgttgtg aagttacgaa atttgaaata
      841 gtctcaaatt aacatgaaat agtctcaaat taacatgaac ctgcatgaaa atactccatg
      901 catcaccgtg agaaaacaca accaatttgg actcattgtt gatagagtta cgtgtcgatt
      961 tagaatagtc cgtgcacccg tgaaattatt gtgcactcaa actttacttt actcattttt
     1021 cgtttttcat ttttcaaata tcgaattcat gtgtgagcta gaatcaaaat atgccttgaa
     1081 gattcccaat cttatacaat tgaagtgaca acgcgcattc aagcagatct cagacttttg
     1141 ttttccaaat cccttaacac aatactaaaa ggaaggtttt aaatgaagat tattatattt
     1201 gtctggcgat ttttttttat cagacaatac aaaagagaat agttcctata taaagtagca
     1261 gaaatttttt tttattcgaa ctcatctcta agatttttga ctgcaagact cgttatcgcg
     1321 taattcaagt aagttttgaa aaatgctcgt acgcaatttt tcaattgaaa gcgacatcat
     1381 tcaatgaata cgttaacaaa tttgataatc gcgagttgtg tagtctattc ttgacatttc
     1441 gtcacaactt tttacaaata ttaccatttc aaattgtcta acagaacaaa tttcgaatcg
     1501 tttttaaaca aacggtaaga caaatttccg ggtatacgtg ctaaaaactt gttctaagac
     1561 cataagaata catatttttc ggacccggga ttggccaaag gacccaaagg tatgtttcga
     1621 atgatactaa cataacatag aacattttca ggaggaccct tgcttggagg gtaccgagct
     1681 cagaaaaaat gaccgctcca aagaagaagc gcaaggttcc agtcgagaag atgactgccc
     1741 caaaaaagaa acgtaaggtc ccgcgggtaa gtttaaacag ttcggtacta actaaccata
     1801 catatttaaa ttttcagcgc gctgtgtcaa aaggagaaga gcttattaag gagaatatgc
     1861 gttccaagct ctacctcgag ggatccgtca acggacacca attcaagtgc acccacgagg
     1921 gagagggaaa gccatacgag ggaaagcaaa ccaaccgtat caaggtcgtc gagggaggac
     1981 cactcccatt cgccttcgac atcctcgcca cccacttcat gtacggatcc aaggtaagtt
     2041 taaacatata tatactaact aaccctgatt atttaaattt tcaggtcttc atcaagtacc
     2101 cagccgacct cccagactac ttcaagcaat ccttcccaga gggattcacc tgggagcgtg
     2161 tcatggtctt cgaggacgga ggagtcctca ccgccaccca agacacctcc ctccaagacg
     2221 gagagctcat ctacaacgtc aaggtccgtg gagtcaactt cccagccaac ggaccagtca
     2281 tgcaaaagaa gaccctcgga tgggagccat ccaccgagac catgtaccca gccgacggag
     2341 gactcgaggg acgttgcgac aaggtaagtt taaacatgat tttactaact aactaatctg
     2401 atttaaattt tcaggccctc aagctcgtcg gaggaggaca cctccacgtc aacttcaaga
     2461 ccacctacaa gtccaagaag ccagtcaaga tgccaggagt ccactacgtc gaccgtcgtc
     2521 tcgagcgtat caaggaggcc gacaacgaga cctacgtcga gcaatacgag cacgccgtcg
     2581 cccgttactc caacctcgga ggaggaatgg acgagctcta caagcctagg ataccggtgg
     2641 gtgaagaccc accaaagcca tctgccaagg gagccaagaa ggccgccaag accgttacga
     2701 agccaaagga cggaaagaag agacgtcatg cccgtaagga atcatactcc gtctacatct
     2761 accgtgtcct caagcaagtt catccagaca ctggagtttc ctccaaagcc atgtctatca
     2821 tgaactcttt tgtcaacgat gtcttcgagc gtattgctgc tgaagcatcc cgtcttgctc
     2881 actacaacaa gcgttccaca atctcatccc gcgaaattca gaccgctgtc cgtctgatcc
     2941 ttccaggaga gcttgccaag cacgccgtgt ctgagggaac caaggccgtt accaagtaca
     3001 cttccagcaa gtaggaattc caactgagcg ccggtcgcta ccattaccaa cttgtctggt
     3061 gtcaaaaata ataggggccg ctgtcatcag agtaagttta aactgagttc tactaactaa
     3121 cgagtaatat ttaaattttc agcatctcgc gcccgtgcct ctgacttcta agtccaatta
     3181 ctcttcaaca tccctacatg ctctttctcc ctgtgctccc accccctatt tttgttatta
     3241 tcaaaaaact tctcttaatt tctttgtttt ttagcttctt ttaagtcacc tctaacaatg
     3301 aaattgtgta gattcaaaaa tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct
     3361 ccctcccccc attaataata attctatccc aaaatctaca caatgttctg tgtacacttc
     3421 ttatgttttt tacttctgat aaattttttt gaaacatcat agaaaaaacc gcacacaaaa
     3481 taccttatca tatgttacgt ttcagtttat gaccgcaatt tttatttctt cgcacgtctg
     3541 ggcctctcat gacgtcaaat catgctcatc gtgaaaaagt tttggagtat ttttggaatt
     3601 tttcaatcaa gtgaaagttt atgaaattaa ttttcctgct tttgcttttt ggggtttccc
     3661 ctattgtttg tcaagatttc gaggacggcg tttttcttgc taaaatcaca agtattgatg
     3721 agcacgatgc aagaaagatc ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta
     3781 gaagttgata atttgaaagt ggagtagtgt ctatggggtt tttgccttaa atgacagaat
     3841 acattcccaa tataccaaac ataactgttt cctactagtc ggccgtacgg gccctttcgt
     3901 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     3961 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     4021 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
     4081 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgg
     4141 ccttaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     4201 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4261 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4321 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4381 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4441 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4501 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4561 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4621 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4681 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4741 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4801 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4861 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4921 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4981 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5041 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5101 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5161 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5221 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5281 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5341 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5401 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5521 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5581 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5641 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5701 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5761 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5821 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5881 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5941 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6001 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6061 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6121 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6181 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6241 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6301 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaatt
//
