LOCUS       sequence-376118-        7487 bp    DNA     circular SYN 15-APR-2025
DEFINITION  gpa-13 fluorescent neural reporter driving nuclear mNeptune2.5 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY94
FEATURES             Location/Qualifiers
     source          1..7487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3162..3182
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3207..3227
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3249..3269
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5078..5100)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5200..5219
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(5297..5315)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5383..5487
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5488..5556,5557..6348)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5706..5725)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6519..7107
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7008..7027
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7395..7416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7431..7436,7437..7454,7455..7461)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7469..7485
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(7474..7487,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagg atgtcgcctg
      181 taaacgatca ttgaaggaaa ttaacgaaga actttaaatt ttgtaaataa attaaaaata
      241 agagcttgta taaaatatct ctattcattt ttatgatttt cgaaaaaaaa ataaaaggca
      301 gagtagagtt tgtgggaaat atgtagcaaa aaataaaaat aaactttatc aaaaatttca
      361 agttgtcaaa tgccagtata tttgaaactt aaattttcgt tgccgaggtt attcaggtcc
      421 agcaaagttt ggtattttgg gaggttcccg aatgcggcaa atttgattgg ccgtggtgat
      481 ggcgtcagtg ttcaaaatag aaatttcata tttgtatgtg gtttcctaga tttcttcttt
      541 ttgattgcat gcattgacgt atttgtgaga gcagttccgt gggagtttct agacaataat
      601 atcaagctgt tgaattgaat tttgcaaata tgatatacat taaaaatttg aaacttgcac
      661 actccagact gaacagatta aaattaatga tgtgagtttc aggtacaata ggtattttct
      721 atttaagaaa ttatgaaacc gtacgcggta atacaacata tgagtcagaa taacttccat
      781 agagggcgcg tcattaacaa ttttgatttt ttcttctttt tccagattta tactctaatt
      841 tttcaaatac ttacgtcatc ataactttaa tttgatatat aaatacaatg cgttgtatgt
      901 atatagtcag aaatatacat tttgtgaaca tataactaga gaaagataga atggaataca
      961 agtgtagaat atggactata cttcagatat tttttttaac ttatagattt tgtttaattt
     1021 acagtttttg tccaaaaatg acaatttcct cttttgattg catttagata taatttgcag
     1081 ggatctaaac aaattggatc aaataatata aaagtttgat caacaattct gactaatcta
     1141 aacgtctagt tggaaagctt cattggaaat atttggcaga tgttttagac ttttcaggtt
     1201 ggcaccgttt atttacaggt gccaataaaa agtggcgttt tcatgttcta atctacgata
     1261 tacgatatac gatactcgtt ttgatttgaa ttattaaaaa aattcatatc aagcatcaaa
     1321 atttaacaag ttccttcggg aggattaacc atctcgtctc aaatttttca tatactgact
     1381 cacagaaatt aatcttgtaa aatctaaccc aactgcttgt gaattaatct aatctctaaa
     1441 gataccttct gaattcgtca acctttttcc tccaatttct tccattgaga cgacgacatc
     1501 taatctcgtt ttctttctca cgagccacat tgatttatgt gatagagtga gcccactggg
     1561 gtgctatgct ttttcacttt tttcttttca gctcacactc cgttctacgt ctctcatgtc
     1621 aattttcaaa ccatatcatt aaatatttat tgactttata aaacatgtca tttgcttata
     1681 gaaacatttt attctaaagt gtgattggaa tgaaaatttg aagcagtgac tgcaaaatat
     1741 gtatcttcgc aaaggagaac taccgtatac agataatccc ttacaaaatt ttgtatttaa
     1801 atatgggcgg aatggaagaa ctatataccc gatattttag attttagatt tttaaactca
     1861 aatctctgca attaatacta actgaacgaa ttctgatatt gcttatgata ttgcttaaat
     1921 acacatatgt atttgagcac agattttagc agtacgttca acaatggtgt tcaaaaataa
     1981 ctcaattgta tggtatcttt ctgaagctta gtaaaaataa tttcaaactg agatcagctg
     2041 tcagtgaagc tgaattcgtc attaatttct aggtttgttg gccacttggc ttgaatttaa
     2101 attttaatta taataggaat tcaattaaga agcgataatt ggtggtttgc agcaggggtg
     2161 tgcggcaaat ttgccgaatt tgccgaattt gccgtttgcc gagctcggca aatttgccga
     2221 atttgccgtg ctcggcaaat attgaaaaaa gagatttgcc gaatttgccg agctccgcaa
     2281 attttgagat ttgccgcaca cccctggttt gcagttaaag caaatttata caactagaca
     2341 aactcagaaa acttgcacgc gttcataatc ataaccaaac ttggggaagt tttttttgtc
     2401 caagaataaa catgaaagga gggaaactgt acttgtcgcg ttattgaaaa aatttgtaac
     2461 ttttacaaat atttttgata aactaagttt gaaaaagact aaaaaatatt cacttttaag
     2521 acattagtat tcttgcagtc attcatgtat gaaatgacat ggaaaaatat ttcgtggttt
     2581 gatttttcat acgtatgttg actaagtttg gattgtatgt aatcttgaaa cgaataatat
     2641 tttctgatcc aaccgatctc tttttacaca cttcccaatt caactaaagt ttctgaaaaa
     2701 accaaaagcg gtagtggttt ttatttcctc aaagtacgtg gatttcatcc aatcgaccag
     2761 gtattatatt ccttggctta ctgactgact ctctttcttt tatttttctg ctcttttcca
     2821 tttgtatgaa aaatatatcc gcgacgatga aaggaaggaa atcgggtgga attgcgttcc
     2881 tattcatctt ttttcaggca cttcttcgtt tttctttttc atcagaattc gttttttttt
     2941 tgttaaaaaa aaataaatta ttgtaataga atgaatgcca attttcagct ttatcagttt
     3001 aggaaatgtg gtgagcaatt gcacccggga ttggccaaag gacccaaagg tatgtttcga
     3061 atgatactaa cataacatag aacattttca ggaggaccct tggagggtac cggttttccc
     3121 agtcacgacg cgtggtaccg agctcagaaa aaatgaccgc tccaaagaag aaacgcaaag
     3181 taccgagctc agaaaaaatg acggcaccta aaaagaaacg caaagtaccg gtcgccgaga
     3241 agacagcccc aaaaaagaag cgtaaagttc cacgcgtcat gggagtaagt ttaaacagtt
     3301 cggtactaac taaccataca tatttaaatt ttcagcgcgc tgtctccaag ggagaggagc
     3361 tcatcaagga gaacatgcac accaagctct acatggaggg aaccgtcaac aaccaccact
     3421 tcaagtgcac ccacgaaggg gagggcaagc cctacgaggg cacccagacc aacaggatta
     3481 aggtggtgga gggaggcccc ctgccgttcg cattcgacat cctggccacc tgctttatgt
     3541 acgggagcaa gaccttcatc aaccacaccc aaggaatccc agacttcttc aagcaatcct
     3601 tcgtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt cagcctgagg
     3661 gcttcacatg ggagagagtc accacatacg aagacggggg cgtgcttacc gttacccagg
     3721 acaccagcct ccaggacggc tgcttgatct acaacgtcaa gctcagaggg gtgaacttcc
     3781 catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccagt accgagaccc
     3841 tgtaccccgc tgacggcggc ctggaaggca gatgcgacat ggccctgaag ctcgtgggcg
     3901 ggggccacct gcactgcaac ctgaagacca catacagatc caagaaaccc gctaagaacc
     3961 tcaagatgcc aggagtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
     4021 ttcaggtcta ctttgtggac cgcagactgg aaagaatcaa ggaggccgac aatgagacct
     4081 acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc aaactggggc
     4141 acaaacttaa tggcatggac gagctgtaca agtaagcgcc ggtcgctacc attaccaact
     4201 tgtctggtgt caaaaataat aggggccgct gtcatcagag taagtttaaa ctgagttcta
     4261 ctaactaacg agtaatattt aaattttcag catctcgcgc ccgtgcctct gacttctaag
     4321 tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt
     4381 tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc
     4441 taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa
     4501 attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg
     4561 tacacttctt atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc
     4621 acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg
     4681 cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt
     4741 ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg
     4801 ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag
     4861 tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa
     4921 ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggtttt tgccttaaat
     4981 gacagaatac attcccaata taccaaacat aactgtttcc tactagtcgg ccgtacgggc
     5041 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     5101 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     5161 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     5221 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     5281 tcaggcggcc ttaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5341 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5401 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5461 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5521 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5581 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5641 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5701 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     5761 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5821 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5881 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5941 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6001 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6061 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6121 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6181 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6241 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6301 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6361 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6421 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6481 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6541 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6601 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6661 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6721 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6781 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6841 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6901 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6961 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7021 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7081 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7141 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7201 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7261 agcgagtcag tgagcgatga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7321 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7381 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7441 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatt
//
