LOCUS       sequence-376131-        5908 bp    DNA     circular SYN 14-APR-2025
DEFINITION  flp-1 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY15
FEATURES             Location/Qualifiers
     source          1..5908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1273..1293
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1315..1335
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3499..3521)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3621..3640
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3718..3736)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3804..3908
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3909..3977,3978..4769)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4127..4146)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4940..5528
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5429..5448
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5682..5699
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5816..5837
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5852..5857,5858..5875,5876..5882)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5890..5906
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(5895..5908,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgcctgcagc agtggaccat
      181 aatccaaact cattcaatgc acaagttaat agaatttgat tgcatggatt gaacatcata
      241 cattggatta cgtctgtgtg actgaaattt ataattacat ttttgcaatt tcgtattacg
      301 aattttgaat tttcaacaaa aattgtaaaa ctacaatttg ctaaaaaaat atggcaatag
      361 ccagattctc agtctgttcg gaaaaattcc aacctgtatc tcaaagttcc ctcgtgtttc
      421 tcattccaca aaattaccaa tttatcagca ccacctgatg caaatagttc gccattatgt
      481 gaccatgcga ctgtgtatac tagatctttg tgtcctgaaa ttcagttgat ttagagttag
      541 agaagggctc aatgaattct tactctaaat aattttaaaa cgtacttacc tttcaaagtt
      601 tgcatttgac caccttcatt aacatcaaac aagtaaactt tgttatctgc agccaacaaa
      661 agttcagagc catctggttt gaatgcgaga tcataaatgc aaactctgga aattaatttc
      721 tatttcaaat gatacgtttt aaattataat tgaggttttc cggttcttaa aaaatatgtg
      781 gtaaaatgat aatctagcta tttaattgtt cacaattttt gaaggaattc ctaaaaaccc
      841 aaaaaaaaaa actgttcccc atggaagcct aattttcttg aaatcttttt catcgtcatc
      901 atcttcctga aattccaatc catagaatgt gtcccgtttt ggatgtataa aacataatgg
      961 aattagtcct cgtcaagcac gtcttacgga ccccagatgc aagaaataca atatgaatat
     1021 taaaatcaaa ttaattattt gtggtaaata tcatcatcat cctactcata ctcatcatgg
     1081 ggggttcaag attggatacg gtacttgcaa agaaggctta taaatagctc acatcttcat
     1141 tggttttctt cactttatcc cgggattggc caaaggaccc aaaggtatgt ttcgaatgat
     1201 actaacataa catagaacat tttcaggagg acccttgctt ggagggtacc gagctcagaa
     1261 aaaatgaccg ctccaaagaa gaagcgcaag gttccagtcg agaagatgac tgccccaaaa
     1321 aagaaacgta aggtcccgcg gatgtcagag cttattaagg agaatatgca tatgaaactg
     1381 tacatggaag gaaccgtgga caaccatcac tttaagtgca cttcggaggg ggaaggcaaa
     1441 ccgtacgagg gcacccagac catgcgaatc aaggtggtcg agggaggccc actgcccttc
     1501 gccttcgaca ttctggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat
     1561 tttcaggcta ctagcttcct gtacggcagc aagactttta ttaatcacac ccagggcatc
     1621 ccggacttct tcaaacagtc gttccctgag ggcttcacat gggagcgtgt gaccacatac
     1681 gaagacggtg gcgttctcac cgctacccag gacaccagcc tccaggacgg ttgcctcata
     1741 tataatgtgg taagtttaaa cagttcggta ctaactaacc atacatattt aaattttcag
     1801 aagatccggg gagtaaactt cacatccaat ggtccggtta tgcagaagaa aacactcggc
     1861 tgggaggcct tcaccgagac gctgtatccg gctgacggag gcctggaagg tcgaaacgac
     1921 atggccctga agctcgtcgg cggtagccat ctcatcgcaa acgctaagac cacatataga
     1981 tccaagaaac cggtaagttt aaacatgatt ttactaacta actaatctga tttaaatttt
     2041 caggctaaga accttaaaat gccgggagtc tactatgttg actacagact ggaacggatc
     2101 aaggaggcca acaacgagac ctacgtcgag cagcacgagg ttgcagttgc cagatactgc
     2161 gacctcccga gcaaactggg tcacaagctt aatcctagga taccggtggg tgaagaccca
     2221 ccaaagccat ctgccaaggg agccaagaag gccgccaaga ccgttacgaa gccaaaggac
     2281 ggaaagaaga gacgtcatgc ccgtaaggaa tcatactccg tctacatcta ccgtgtcctc
     2341 aagcaagttc atccagacac tggagtttcc tccaaagcca tgtctatcat gaactctttt
     2401 gtcaacgatg tcttcgagcg tattgctgct gaagcatccc gtcttgctca ctacaacaag
     2461 cgttccacaa tctcatcccg cgaaattcag accgctgtcc gtctgatcct tccaggagag
     2521 cttgccaagc acgccgtgtc tgagggaacc aaggccgtta ccaagtacac ttccagcaag
     2581 taggaattcc aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa
     2641 taggggccgc tgtcatcaga gtaagtttaa actgagttct actaactaac gagtaatatt
     2701 taaattttca gcatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat
     2761 ccctacatgc tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt
     2821 ctcttaattt ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag
     2881 attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca
     2941 ttaataataa ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt
     3001 acttctgata aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat
     3061 atgttacgtt tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg
     3121 acgtcaaatc atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag
     3181 tgaaagttta tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt
     3241 caagatttcg aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca
     3301 agaaagatcg gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa
     3361 tttgaaagtg gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat
     3421 ataccaaaca taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt
     3481 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
     3541 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
     3601 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     3661 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc
     3721 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     3781 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3841 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3901 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3961 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     4021 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     4081 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     4141 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     4201 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     4261 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4321 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4381 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4441 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4501 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4561 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4621 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4681 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4741 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4801 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4861 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4921 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4981 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     5041 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     5101 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     5161 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     5221 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     5281 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5341 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5401 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5461 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5521 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5581 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5641 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5701 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     5761 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     5821 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     5881 tgtgtggaat tgtgagcgga taacaatt
//
