LOCUS       sequence-376266-        9064 bp    DNA     circular SYN 14-APR-2025
DEFINITION  flp-18 fluorescent neural reporter driving nuclear mTagBFP2 
            expression (refer to NeuroPAL paper for expression).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9064)
  AUTHORS   Yemini E, Lin A, Nejatbakhsh A, Varol E, Sun R, Mena GE, Samuel ADT,
            Paninski L, Venkatachalam V, Hobert O
  TITLE     NeuroPAL: A Multicolor Atlas for Whole-Brain Neuronal Identification
            in C. elegans.
  JOURNAL   Cell. 2021 Jan 7;184(1):272-288.e11. doi: 
            10.1016/j.cell.2020.12.012. Epub 2020 Dec 29.
  PUBMED    33378642
REFERENCE   2  (bases 1 to 9064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.12.012"; journalName: "Cell"; date: "2021-01-7-
            7"; volume: "184"; issue: "1"; pages: "272-288.e11"
COMMENT     Sequence Label: pEY17
FEATURES             Location/Qualifiers
     source          1..9064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..22
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6..22
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(3196..3207)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             4429..4449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4471..4491
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6655..6677)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6777..6796
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6874..6892)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6960..7064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7065..7133,7134..7925)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7283..7302)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8096..8684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8585..8604
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8838..8855
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8972..8993
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9008..9013,9014..9031,9032..9038)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9046..9062
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(9051..9064,1..9)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tcacacagga aacagctatg accatgatta cgccaagctg taagtttaaa catgatctta
       61 ctaactaact attctcattt aaattttcag agcttaaaaa tggctgaaat cactcacaac
      121 gatggatacg ctaacaactt ggaaatgaaa taagcttgca tgctctgtca catactgctc
      181 gaatcggagg taggtttgaa aaacatgcaa ttcatttcca aaatatgacg aattttgaaa
      241 aaaaaaaata gaaaagaaac tcaattaaat taccttattt tttctagtac gtcaattttg
      301 actcgcactt aaaatctgca ttttaaaaat gaaaattcag tgcagctgca gtgcagtgcg
      361 gctattattt tcggcatttt tgcaaaactt acacaccacc gttataagaa aatatttgtc
      421 ttttttttta aatttttatt cagtcgttat tcgaaaaaga cttacagtac cttgctgaat
      481 tccttccgca ttgcgctaaa atttaccgat tgcaaaataa tcttacatgc tatagtaata
      541 aaaaatttac atacttgcca tttcatttca tttaattatt tcatccaatt tatcttgatc
      601 agtattactc tttgttaaaa attccactgt tcaattttta catttcagtt gttttaatat
      661 cgaagtgcac aagaagcgcg aacataatgg acaccatcgc tcaaaaatta tgcttctagt
      721 tgtagttcct cgagcatttt tgatctcaaa agacatttcc aagagcaata attgccattt
      781 ttgagctaca agtaccatat cctgctcata attcatgttc caccactccc taaagttttt
      841 tctttggaat tccccagact ttgcgcctaa aattctcgtt attctctcca attcaattgg
      901 ctcaattcaa ctgactagat ttcagtgatg taggtagcgc ggaactcagt attaagagag
      961 gactcaactc agtttctaat ccaatttcaa caaattctaa gacaccgaaa cgctgcaggt
     1021 ctcgtagagc ctacgtgttg acaggttctt ccttttcaat ctatttccgt cgatattccg
     1081 gcgtcattta gttgaaaatg agtgaaaaaa tgcgatctga cgcgaattca cgtgaactcg
     1141 aaaattcgag aatgtaatca ttaagtttcc aaacaccaag ttctctttcg aaaccaagtc
     1201 aatagaaaaa tgagacaccc acgagaagaa ggttggtccg aaaaaagaaa ctaaagaagt
     1261 tatttatttc tctttttgat gtttgttgag cagaatatga tttagttaag cacatctgat
     1321 ttcaacgccg atgaatgagg tcgtaggcgt cacgttcaat ttgatctgat ttgttcagtg
     1381 cttacgagta gaaattctaa atatgatgtt actttttggt tctgtaagat ggagctgggt
     1441 atacatgtgg tgcacggcaa tcgctatttg gcaatttcag tcatttcggc aaattcggca
     1501 attttggcga attctcatat tttggaaatt ttggcaaatg cggaaaattt caaaaacaag
     1561 caattaccaa atttgccaaa cgccgaaaaa tttaaaaaaa aaccgacatt tgctggtatt
     1621 tttttttaat caggatgcta attatgtaag gttaatctat aaattcttaa aacacactta
     1681 actttgaaat tgaaatcact gacatttcgc gcattcgccg ataaaatctc taaataaaat
     1741 aatgtaaact gtgtccagtt ctggtacttg tttagtatac ctggttcaaa atattttcca
     1801 atgcgttttc catttattta ttttagaaat gatcatttaa ttaaaacgta aaattcgcat
     1861 tttcaagctt agaaacatta atcctaagtc caatatggac aaaaagagta gtgtattagt
     1921 accaagtaag aaccctaaaa aaaaaatatt ctaattaaat ttttggcaaa atgccacatt
     1981 tttctttttt ttctcaacgg ggaaaagttg tgtttcttcc ctttttatta aattttttgt
     2041 tttccttagg ctaagtacaa cttgaaatgg aacagaaatt acataaattt attgctttca
     2101 cattttgctc ttcttcactc aaatcttgga ctcttgcttt tcacaaaaaa aaccttataa
     2161 acaaaaataa gacatctgaa aaaatgatga acttcctgtc agtttgttcc agtatccttc
     2221 ttaaatttcc ttgttttcac atcacaaaaa tcctacatca cgcttcgact catttcctga
     2281 cagtcatcac atcaccaaat gaatttctat ttttacaata tagttgtagt atttttgtcg
     2341 aacatatatt gttctaacta acttcttaaa aaattgtgct cttgactgaa cacaaatttc
     2401 tgcgtcgttt cacgtttact tttctcaatt ttttaaaacc aaaatttaac tgtaaacttc
     2461 tgcattgttc taaaaactca caggttatct caaaaagata aatttcaaag tgagattttt
     2521 ttaaaaaaat tccggactgt tttttgattt tgatgttttt aatgaatttt taaaattctt
     2581 tattagtgcc tgaaattaac aataatttta aagtttgtca cactgcgaat aaattaatct
     2641 cacatttcgt acgtttagaa agatgcatga aactccgtcg ttcttatgaa gttaattaag
     2701 acatggctaa ttgaaagccc acatggtcat gaaaaagtaa aacttacaac tttttactga
     2761 atattaacca gtctctataa atatcccagc ttagccggaa tagggtcacg aaaacatgaa
     2821 aataaagctt gaacctattg ctatgatttt caatgccacc ttttgcagtt taatatacac
     2881 tattactact tctcagttca agttcacttc tgagacggaa cctttatttt ctaactttct
     2941 ttaatcttac tttttatgag ccgaatgttc tattttgtaa aatgatcaga tacaagccca
     3001 aagctaattt tgccaataag tgttagtaga tcaattgaat tacaatataa tgacctgggc
     3061 ggaatagatt tttgcactga tttttttcca agcttactct atgaatattc atagatcatc
     3121 ttcaaaggta gcgtgtgttg tagttgtgtt ttgtcgggtt cgtgttattt gactcagctc
     3181 aagtagatgt tgtagtcttc cctcaatcaa cgcgtgggct tgaccctctt taaattgagt
     3241 gaaaaccagc caatctattt gaaatctacc cccgaaatga gattcgacga tgacacgacg
     3301 tgtgtgttac gtgcgctgag aatttaggaa aaaaaagata atcttctttt cgttagctgc
     3361 gccacaacct gtgcagataa actccgcaca attgaagttc ttactggtcc cacccgattt
     3421 attcaattgt attgtgtctt tttttcttat ttctctacaa ctctaacttt cttcaactat
     3481 tctctacatc agtgcttttt tccaccatga aaaaagaaat gacgtcgttg tttcttagga
     3541 ggggcggagc ctcaactcaa tggtccgttc gaatagatcc cacccctcat ataaatagga
     3601 ctgaaaccaa agaagttttt aaattttcag cttaccgtgt ttctcaattt tcaaaatagt
     3661 cttttttgtg tctgaacggg ctgatcaatt atgttttttt ttgaatgaaa aatcttcctc
     3721 ccaagcctta aaattccttc caaagatcga aagttatgtc tattcaagtt acttcttctt
     3781 agatcaaata ttaaacaaaa aaatacgcca tcattctctc acatttagta attcaaaatt
     3841 tcgatttgtt ttcgaacttt tgaatttccc gcctaggaag tgacatgagt gaaatctcat
     3901 tagataaatg tgccactcac cgatgacacc attttcaaat aagggggggg ggtgtttcga
     3961 ataccttgta cacgcgtttc cgcaaaacca cacaccccga gttcctcagc ttcttcccct
     4021 cgacccatga gccacgtcaa gatgtcagcg tgtcactttt cattgcttca ccttctacgg
     4081 taacgagaaa aggcggggaa tccaggatct ggtgtgactt cttccttctt cgtatcttcc
     4141 taaaaatgtt cgtttacttt ttagcgagtg tcatttgatg ggatgtcgtt cttgaggaaa
     4201 gaggctagaa gcttaatact ctcatatttc atattattgg catcaagacg tttattattt
     4261 aactacaata acgattgctt taacaactaa aaataataat ttcagggtta gacacccggg
     4321 attggccaaa ggacccaaag gtatgtttcg aatgatacta acataacata gaacattttc
     4381 aggaggaccc ttgcttggag ggtaccgagc tcagaaaaaa tgaccgctcc aaagaagaag
     4441 cgcaaggttc cagtcgagaa gatgactgcc ccaaaaaaga aacgtaaggt cccgcggatg
     4501 tcagagctta ttaaggagaa tatgcatatg aaactgtaca tggaaggaac cgtggacaac
     4561 catcacttta agtgcacttc ggagggggaa ggcaaaccgt acgagggcac ccagaccatg
     4621 cgaatcaagg tggtcgaggg aggcccactg cccttcgcct tcgacattct ggtaagttta
     4681 aacatatata tactaactaa ccctgattat ttaaattttc aggctactag cttcctgtac
     4741 ggcagcaaga cttttattaa tcacacccag ggcatcccgg acttcttcaa acagtcgttc
     4801 cctgagggct tcacatggga gcgtgtgacc acatacgaag acggtggcgt tctcaccgct
     4861 acccaggaca ccagcctcca ggacggttgc ctcatatata atgtggtaag tttaaacagt
     4921 tcggtactaa ctaaccatac atatttaaat tttcagaaga tccggggagt aaacttcaca
     4981 tccaatggtc cggttatgca gaagaaaaca ctcggctggg aggccttcac cgagacgctg
     5041 tatccggctg acggaggcct ggaaggtcga aacgacatgg ccctgaagct cgtcggcggt
     5101 agccatctca tcgcaaacgc taagaccaca tatagatcca agaaaccggt aagtttaaac
     5161 atgattttac taactaacta atctgattta aattttcagg ctaagaacct taaaatgccg
     5221 ggagtctact atgttgacta cagactggaa cggatcaagg aggccaacaa cgagacctac
     5281 gtcgagcagc acgaggttgc agttgccaga tactgcgacc tcccgagcaa actgggtcac
     5341 aagcttaatc ctaggatacc ggtgggtgaa gacccaccaa agccatctgc caagggagcc
     5401 aagaaggccg ccaagaccgt tacgaagcca aaggacggaa agaagagacg tcatgcccgt
     5461 aaggaatcat actccgtcta catctaccgt gtcctcaagc aagttcatcc agacactgga
     5521 gtttcctcca aagccatgtc tatcatgaac tcttttgtca acgatgtctt cgagcgtatt
     5581 gctgctgaag catcccgtct tgctcactac aacaagcgtt ccacaatctc atcccgcgaa
     5641 attcagaccg ctgtccgtct gatccttcca ggagagcttg ccaagcacgc cgtgtctgag
     5701 ggaaccaagg ccgttaccaa gtacacttcc agcaagtagg aattccaact gagcgccggt
     5761 cgctaccatt accaacttgt ctggtgtcaa aaataatagg ggccgctgtc atcagagtaa
     5821 gtttaaactg agttctacta actaacgagt aatatttaaa ttttcagcat ctcgcgcccg
     5881 tgcctctgac ttctaagtcc aattactctt caacatccct acatgctctt tctccctgtg
     5941 ctcccacccc ctatttttgt tattatcaaa aaacttctct taatttcttt gttttttagc
     6001 ttcttttaag tcacctctaa caatgaaatt gtgtagattc aaaaatagaa ttaattcgta
     6061 ataaaaagtc gaaaaaaatt gtgctccctc cccccattaa taataattct atcccaaaat
     6121 ctacacaatg ttctgtgtac acttcttatg ttttttactt ctgataaatt tttttgaaac
     6181 atcatagaaa aaaccgcaca caaaatacct tatcatatgt tacgtttcag tttatgaccg
     6241 caatttttat ttcttcgcac gtctgggcct ctcatgacgt caaatcatgc tcatcgtgaa
     6301 aaagttttgg agtatttttg gaatttttca atcaagtgaa agtttatgaa attaattttc
     6361 ctgcttttgc tttttggggt ttcccctatt gtttgtcaag atttcgagga cggcgttttt
     6421 cttgctaaaa tcacaagtat tgatgagcac gatgcaagaa agatcggaag aaggtttggg
     6481 tttgaggctc agtggaaggt gagtagaagt tgataatttg aaagtggagt agtgtctatg
     6541 gggtttttgc cttaaatgac agaatacatt cccaatatac caaacataac tgtttcctac
     6601 tagtcggccg tacgggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     6661 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     6721 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     6781 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     6841 aaggagaaaa taccgcatca ggcggcctta agggcctcgt gatacgccta tttttatagg
     6901 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     6961 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     7021 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     7081 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     7141 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     7201 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     7261 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     7321 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     7381 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     7441 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     7501 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     7561 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     7621 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     7681 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     7741 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     7801 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     7861 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     7921 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     7981 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     8041 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8101 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8161 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8221 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     8281 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8341 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8401 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8461 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     8521 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     8581 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     8641 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     8701 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     8761 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     8821 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     8881 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     8941 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     9001 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9061 aatt
//
