LOCUS       sequence-376461-        8522 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Cu2+-dependent expression of N-terminal FUS fused with red 
            fluorescent protein in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8522)
  AUTHORS   Utsumi R, Murata Y, Ito-Harashima S, Akai M, Miura N, Kuroda K, Ueda
            M, Kataoka M
  TITLE     Foci-forming regions of pyruvate kinase and enolase at the molecular
            surface incorporate proteins into yeast cytoplasmic metabolic 
            enzymes transiently assembling (META) bodies.
  JOURNAL   PLoS One. 2023 Apr 13;18(4):e0283002. doi: 
            10.1371/journal.pone.0283002. eCollection 2023.
  PUBMED    37053166
REFERENCE   2  (bases 1 to 8522)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2023-04-13"; volume: "18(4):e0283002. doi"; pages: " 
            10.1371/journal.pone.0283002. eCollection 2023"
COMMENT     Sequence Label: p426-Cup1p-FUS-FusionRed
FEATURES             Location/Qualifiers
     source          1..8522
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..39)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      80..1422
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    412..459
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        1449..1553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1554..1622,1623..2414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1772..1791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2585..3173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3074..3093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3327..3344
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3461..3482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3497..3502,3503..3520,3521..3527)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3535..3551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3540..3562
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3559..3575
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3559..3575
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3594..3614
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3596..3614
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3638..3872
                     /gene="S. cerevisiae CUP1"
                     /label=CUP1 promoter
                     /note="inducible metallothionein promoter, regulated by 
                     Cu2+"
     primer_bind     3887..3903
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3887..3903
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    3911..3935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4321..4332
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5523..5547)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5579..6277
                     /codon_start=1
                     /product="monomeric red fluorescent protein optimized for 
                     use as a fusion tag (Shemiakina et al., 2012)"
                     /label=FusionRed
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
                     KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
                     STGGAGDGGK"
     terminator      6284..6531
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(6300..6318)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(6549..6568)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6550..6568)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6578..6595)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6578..6594)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6587..6609)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6735..7190
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6822..6841)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7032..7053
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7321..8124)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8125..8340)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8364..8383)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8483..8505
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
       61 aataatggtt tcttagtatg atccaatatc aaaggaaatg atagcattga aggatgagac
      121 taatccaatt gaggagtggc agcatataga acagctaaag ggtagtgctg aaggaagcat
      181 acgatacccc gcatggaatg ggataatatc acaggaggta ctagactacc tttcatccta
      241 cataaataga cgcatataag tacgcattta agcataaaca cgcactatgc cgttcttctc
      301 atgtatatat atatacaggc aacacgcaga tataggtgcg acgtgaacag tgagctgtat
      361 gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt tgaagttcct
      421 attccgaagt tcctattctc tagaaagtat aggaacttca gagcgctttt gaaaaccaaa
      481 agcgctctga agacgcactt tcaaaaaacc aaaaacgcac cggactgtaa cgagctacta
      541 aaatattgcg aataccgctt ccacaaacat tgctcaaaag tatctctttg ctatatatct
      601 ctgtgctata tccctatata acctacccat ccacctttcg ctccttgaac ttgcatctaa
      661 actcgacctc tacatttttt atgtttatct ctagtattac tctttagaca aaaaaattgt
      721 agtaagaact attcatagag tgaatcgaaa acaatacgaa aatgtaaaca tttcctatac
      781 gtagtatata gagacaaaat agaagaaacc gttcataatt ttctgaccaa tgaagaatca
      841 tcaacgctat cactttctgt tcacaaagta tgcgcaatcc acatcggtat agaatataat
      901 cggggatgcc tttatcttga aaaaatgcac ccgcagcttc gctagtaatc agtaaacgcg
      961 ggaagtggag tcaggctttt tttatggaag agaaaataga caccaaagta gccttcttct
     1021 aaccttaacg gacctacagt gcaaaaagtt atcaagagac tgcattatag agcgcacaaa
     1081 ggagaaaaaa agtaatctaa gatgctttgt tagaaaaata gcgctctcgg gatgcatttt
     1141 tgtagaacaa aaaagaagta tagattcttt gttggtaaaa tagcgctctc gcgttgcatt
     1201 tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct ctcgcgttgc
     1261 atttttgttt tacaaaaatg aagcacagat tcttcgttgg taaaatagcg ctttcgcgtt
     1321 gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc
     1381 gttgcatttt tgttctacaa aatgaagcac agatgcttcg ttcaggtggc acttttcggg
     1441 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1501 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1561 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1621 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1681 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1741 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1801 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1861 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1921 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1981 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2041 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2101 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2161 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2221 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2281 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2341 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2401 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2461 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2521 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2581 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2641 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2701 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2761 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2821 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2881 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2941 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3001 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3061 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3121 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3181 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3241 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3301 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3361 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3421 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     3481 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     3541 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     3601 accctcacta aagggaacaa aagctggagc tctagtacta gttagaaaaa gacatttttg
     3661 ctgtcagtca ctgtcaagag attcttttgc tggcatttct tctagaagca aaaagagcga
     3721 tgcgtctttt ccgctgaacc gttccagcaa aaaagactac caacgcaata tggattgtca
     3781 gaatcatata aaagagaagc aaataactcc ttgtcttgta tcaattgcat tataatatct
     3841 tcttgttagt gcaatatcat atagaagtca tcaaaaaacc ccggattcta gaactagtgg
     3901 atcccccatc acaagtttgt acaaaaaagc aggctccacc atggcctcaa acgattatac
     3961 ccaacaagca acccaaagct atggggccta ccccacccag cccgggcagg gctattccca
     4021 gcagagcagt cagccctacg gacagcagag ttacagtggt tatagccagt ccacggacac
     4081 ttcaggctat ggccagagca gctattcttc ttatggccag agccagaaca caggctatgg
     4141 aactcagtca actccccagg gatatggctc gactggcggc tatggcagta gccagagctc
     4201 ccaatcgtct tacgggcagc agtcctccta tcctggctat ggccagcagc cagctcccag
     4261 cagcacctcg ggaagttacg gtagcagttc tcagagcagc agctatgggc agccccagag
     4321 tgggagctac agccagcagc ctagctatgg tggacagcag caaagctatg gacagcagca
     4381 aagctataat ccccctcagg gctatggaca gcagaaccag tacaacagca gcagtggtgg
     4441 tggaggtgga ggtggaggtg gaggtaacta tggccaagat caatcctcca tgagtagtgg
     4501 tggtggcagt ggtggcggtt atggcaatca agaccagagt ggtggaggtg gcagcggtgg
     4561 ctatggacag caggaccgtg gaggccgcgg caggggtggc agtggtggcg gcggcggcgg
     4621 cggcggtggt ggttacaacc gcagcagtgg tggctatgaa cccagaggtc gtggaggtgg
     4681 ccgtggaggc agaggtggca tgggcggaag tgaccgtggt ggcttcaata aatttggtgg
     4741 ccctcgggac caaggatcac gtcatgactc cgaacaggat aattcagaca acaacaccat
     4801 ctttgtgcaa ggcctgggtg agaatgttac aattgagtct gtggctgatt acttcaagca
     4861 gattggtatt attaagacaa acaagaaaac gggacagccc atgattaatt tgtacacaga
     4921 cagggaaact ggcaagctga agggagaggc aacggtctct tttgatgacc caccttcagc
     4981 taaagcagct attgactggt ttgatggtaa agaattctcc ggaaatccta tcaaggtctc
     5041 atttgctact cgccgggcag actttaatcg gggtggtggc aatggtcgtg gaggccgagg
     5101 gcgaggagga cccatgggcc gtggaggcta tggaggtggt ggcagtggtg gtggtggccg
     5161 aggaggattt cccagtggag gtggtggcgg tggaggacag cagcgagctg gtgactggaa
     5221 gtgtcctaat cccacctgtg agaatatgaa cttctcttgg aggaatgaat gcaaccagtg
     5281 taaggcccct aaaccagatg gcccaggagg gggaccaggt ggctctcaca tggggggtaa
     5341 ctacggggat gatcgtcgtg gtggcagagg aggctatgat cgaggcggct accggggccg
     5401 cggcggggac cgtggaggct tccgaggggg ccggggtggt ggggacagag gtggctttgg
     5461 ccctggcaag atggattcca ggggtgagca cagacaggat cgcagggaga ggccgtattt
     5521 ggacccagct ttcttgtaca aagtggtgat gggctgcagg aattcgatat caagcttaat
     5581 ggtgagcgag ctgattaagg agaacatgcc catgaagctg tacatggagg gcaccgtgaa
     5641 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     5701 catgagaatc aaggtcgtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     5761 cagcttcatg tacggcagca gaaccttcat caagcaccct ccgggcatcc ccgacttctt
     5821 taagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     5881 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     5941 ggttagaggg gtgaacttcc cagccaacgg ccctgtgatg cagaagaaaa cactcggctg
     6001 ggaggcctcc accgagacga tgtaccccgc tgacggcggc ctggaaggcg catgtgacat
     6061 ggccctgaag ctcgtgggcg ggggccacct gatctgcaac cttgagacca catacagatc
     6121 caagaaaccc gctacgaacc tcaagatgcc cggcgtctac aacgtggacc acagactgga
     6181 aagaatcaag gaggccgacg atgagaccta cgtcgagcag cacgaggtgg ctgtggccag
     6241 atactctact ggtggcgctg gtgatggagg taaataactc gagtcatgta attagttatg
     6301 tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga
     6361 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
     6421 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
     6481 ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg cggccggtac
     6541 ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc
     6601 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     6661 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     6721 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     6781 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     6841 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     6901 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     6961 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7021 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7081 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7141 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg
     7201 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagggtaata actgatataa
     7261 ttaaattgaa gctctaattt gtgagtttag tatacatgca tttacttata atacagtttt
     7321 ttagttttgc tggccgcatc ttctcaaata tgcttcccag cctgcttttc tgtaacgttc
     7381 accctctacc ttagcatccc ttccctttgc aaatagtcct cttccaacaa taataatgtc
     7441 agatcctgta gagaccacat catccacggt tctatactgt tgacccaatg cgtctccctt
     7501 gtcatctaaa cccacaccgg gtgtcataat caaccaatcg taaccttcat ctcttccacc
     7561 catgtctctt tgagcaataa agccgataac aaaatctttg tcgctcttcg caatgtcaac
     7621 agtaccctta gtatattctc cagtagatag ggagcccttg catgacaatt ctgctaacat
     7681 caaaaggcct ctaggttcct ttgttacttc ttctgccgcc tgcttcaaac cgctaacaat
     7741 acctgggccc accacaccgt gtgcattcgt aatgtctgcc cattctgcta ttctgtatac
     7801 acccgcagag tactgcaatt tgactgtatt accaatgtca gcaaattttc tgtcttcgaa
     7861 gagtaaaaaa ttgtacttgg cggataatgc ctttagcggc ttaactgtgc cctccatgga
     7921 aaaatcagtc aagatatcca catgtgtttt tagtaaacaa attttgggac ctaatgcttc
     7981 aactaactcc agtaattcct tggtggtacg aacatccaat gaagcacaca agtttgtttg
     8041 cttttcgtgc atgatattaa atagcttggc agcaacagga ctaggatgag tagcagcacg
     8101 ttccttatat gtagctttcg acatgattta tcttcgtttc ctgcaggttt ttgttctgtg
     8161 cagttgggtt aagaatactg ggcaatttca tgtttcttca acactacata tgcgtatata
     8221 taccaatcta agtctgtgct ccttccttcg ttcttccttc tgttcggaga ttaccgaatc
     8281 aaaaaaattt caaggaaacc gaaatcaaaa aaaagaataa aaaaaaaatg atgaattgaa
     8341 aaggtggtat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     8401 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     8461 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     8521 cc
//
