LOCUS       sequence-376555-       10156 bp    DNA     circular SYN 14-APR-2025
DEFINITION  SHOC2 variant overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Kwon JJ, Hajian B, Bian Y, Young LC, Amor AJ, Fuller JR, Fraley CV, 
            Sykes AM, So J, Pan J, Baker L, Lee SJ, Wheeler DB, Mayhew DL, 
            Persky NS, Yang X, Root DE, Barsotti AM, Stamford AW, Perry CK, 
            Burgin A, McCormick F, Lemke CT, Hahn WC, Aguirre AJ
  TITLE     Structure-function analysis of the SHOC2-MRAS-PP1C holophosphatase 
            complex.
  JOURNAL   Nature. 2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2.
  PUBMED    35831509
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 Jul 13. pii: 10.1038/s41586-022-04928-2. doi: 
            10.1038/s41586-022-04928-2."
COMMENT     Sequence Label: plx307 SHOC2 V5 WT
FEATURES             Location/Qualifiers
     source          1..10156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    254..375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(291..310)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     345..364
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      415..550
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(570..589)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(571..589)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(599..616)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(599..615)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(608..630)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      757..1212
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(844..863)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1054..1075
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1238..1342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1343..1411,1412..2203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1561..1580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2374..2962
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2863..2882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3116..3133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3250..3271
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3286..3291,3292..3309,3310..3316)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3324..3340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3329..3351
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3348..3364
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3348..3364
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3383..3403
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3385..3403
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3431..3657
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3658..3838
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3885..4010
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4503..4736
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4921..4965
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5196..5406
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5218..5414
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5265..5400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5446..6048
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(5446..5465)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5942..5962
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6091..6208
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6346..7524
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6577..7515
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7472..7492
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7544..7568
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9315..9339)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9347..9388
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    9439..10027
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9492..9512)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9910..9921)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(10099..10156,1..176)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
       61 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      121 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
      181 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
      241 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
      301 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
      361 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
      421 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      481 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
      541 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
      601 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      661 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      721 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
      781 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
      841 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
      901 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
      961 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     1021 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     1081 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     1141 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     1201 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1261 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1321 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1381 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1441 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1501 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1561 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     1621 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1681 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1741 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1801 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1861 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1921 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1981 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2041 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2101 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2161 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2221 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2281 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2341 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2401 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2461 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2521 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2581 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2641 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2701 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2761 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2821 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2881 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2941 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3001 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3061 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3121 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     3181 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     3241 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     3301 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     3361 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     3421 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     3481 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     3541 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     3601 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     3661 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     3721 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     3781 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     3841 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     3901 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     3961 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     4021 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     4081 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     4141 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     4201 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     4261 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     4321 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     4381 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     4441 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     4501 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     4561 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     4621 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     4681 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     4741 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     4801 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     4861 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     4921 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     4981 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     5041 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     5101 ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt
     5161 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     5221 gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     5281 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     5341 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     5401 taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag
     5461 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5521 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5581 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5641 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5701 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5761 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5821 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5881 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5941 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6001 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat
     6061 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     6121 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     6181 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     6241 ttggcgccgg ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat
     6301 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     6361 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     6421 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     6481 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     6541 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     6601 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     6661 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     6721 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     6781 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     6841 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     6901 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     6961 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     7021 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     7081 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     7141 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     7201 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     7261 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     7321 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     7381 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     7441 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     7501 tttcttccat ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa
     7561 agttggcatg agtagtagtt taggaaaaga aaaagactct aaagaaaaag atcccaaagt
     7621 accatcagcc aaggaaagag aaaaggaggc aaaagcctct ggaggttttg ggaaagagag
     7681 caaagaaaaa gaacctaaga ccaaagggaa agatgccaaa gatggaaaga aggactccag
     7741 tgctgcccaa ccaggggtgg cattttcagt tgacaatacg atcaaacggc caaacccagc
     7801 acctgggact agaaaaaaat ccagcaatgc agaggtgatt aaagagctca acaaatgccg
     7861 ggaagagaat tcaatgcgtt tggacttatc caagagatct atacacatat tgccatcatc
     7921 aatcaaagag ttgactcaat taacagaact ttatttatac agtaacaaat tgcagtccct
     7981 cccagcagag gtgggatgtt tagtaaatct catgacactg gctctaagtg aaaattcact
     8041 taccagtttg cctgactctc ttgataactt gaagaagctg cggatgcttg atttacggca
     8101 taataaactg agagaaattc cttcagtggt gtataggctg gattctctca ccactcttta
     8161 tcttcgattt aatcgtataa ctactgtgga aaaggacatc aaaaacttgt caaaactcag
     8221 catgcttagc attcgagaga acaaaattaa acaactacct gctgaaattg gtgaattatg
     8281 taatctcatc acgctggatg tagctcacaa tcaacttgaa caccttccaa aggagattgg
     8341 aaactgtaca cagataacca accttgactt gcagcacaat gaactgctag acctcccaga
     8401 tactatagga aacctgtcca gtttaagtcg tcttggtctg agatataaca gactgtcagc
     8461 aatacccaga tcattagcaa aatgcagtgc acttgaagaa ttaaatttag agaacaataa
     8521 catttctact ttaccagaga gtcttttatc aagtcttgtg aaactgaata gtttgacctt
     8581 agctagaaat tgcttccagt tgtatccagt gggtggtcca tctcagtttt ctaccatcta
     8641 ttccctcaac atggaacaca atcgaatcaa caaaattcca tttggaattt tctccagagc
     8701 aaaagtatta agtaagctga atatgaagga caatcagtta acatcacttc ccttggattt
     8761 tggaacttgg accagtatgg tagaattgaa tttagccact aatcagctca caaagatccc
     8821 tgaggatgtg tctggtctcg tttctcttga ggttcttatc ttatcaaaca atcttctaaa
     8881 gaagcttccc catggtcttg gaaaccttag gaagttaaga gagttggatc tagaagagaa
     8941 caaattggaa tccttgccaa atgaaattgc atatcttaag gatttacaga aattagtctt
     9001 gacaaacaac cagttgacca ctcttcccag aggcattggt caccttacta atctcacaca
     9061 tctgggcctt ggagagaacc tacttactca ccttcctgaa gaaattggta cactggagaa
     9121 cctagaagaa ctgtatttga atgacaaccc caacctgcat agccttccct ttgagctggc
     9181 actctgcagc aagctttcaa tcatgagtat tgagaactgt ccactcagtc accttccacc
     9241 tcagattgtt gctggggggc cttctttcat cattcagttc ttaaagatgc agggtccata
     9301 tcgtgccatg gtctgcccaa ctttcttgta caaagtggtt gatatcggta agcctatccc
     9361 taaccctctc ctcggtctcg attctacgta gtaatgaact agtaccggtt aagtcgacaa
     9421 tcaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     9481 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     9541 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     9601 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     9661 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     9721 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     9781 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     9841 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
     9901 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     9961 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    10021 tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
    10081 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt
    10141 ttgcttgtac tgggtc
//
