LOCUS       sequence-377160-       10600 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Expression of AAV2 Rep78-III in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10600)
  AUTHORS   Li P, Marino MP, Zou J, Argaw T, Morreale MT, Iaffaldano BJ, Reiser 
            J
  TITLE     Efficiency and Specificity of Targeted Integration Mediated by the 
            Adeno-Associated Virus Serotype 2 Rep 78 Protein.
  JOURNAL   Hum Gene Ther Methods. 2018 Jun;29(3):135-145. doi: 
            10.1089/hgtb.2018.052.
  PUBMED    29860898
REFERENCE   2  (bases 1 to 10600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1089/hgtb.2018";
            journalName: "Hum Gene Ther Methods"; date: "2018-06"; volume: "29";
            issue: "3"; pages: "135-145"
COMMENT     Sequence Label: pNL(CMV)Rep78-III/CMV/WPRE-Delta-U3
FEATURES             Location/Qualifiers
     source          1..10600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     634..650
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     634..650
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        673..691
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     673..690
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     protein_bind    716..740
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2667..2691)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2722..2741)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2723..2741)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2752..2769)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2752..2768)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2761..2783)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    2800..3388
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2853..2873)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3271..3282)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3463..3696
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5336..5353)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5507..6095)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5587..5606)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6266..7057,7058..7126))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6889..6908
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7127..7231)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7299..7317
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7355..7377)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7477..7496
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     LTR             8649..8829
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      9456..9591
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    9869..10102
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10287..10331
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccggactct
      601 agcctaggcc gcgggacgga taacaatttc acacaggaaa cagctatgac cattaggcct
      661 ttgcaaaaag ctatttaggt gacactatag aaggtacgcc tgcaggtacc ggatcacaag
      721 tttgtacaaa aaagcaggct ggcgccggaa ccaattcagt cgactggatc cggtaccatg
      781 ccggggtttt acgagattgt gattaaggtc cccagcgacc ttgacgagca tctgcccggc
      841 atttctgaca gctttgtgaa ctgggtggcc gagaaggaat gggagttgcc gccagattct
      901 gacatggatc tgaatctgat tgagcaggca cccctgaccg tggccgagaa gctgcagcgc
      961 gactttctga cggaatggcg ccgtgtgagt aaggccccgg aggccctttt ctttgtgcaa
     1021 tttgagaagg gagagagcta ctttcatatg catgtgttgg tggagactac gggagtgaag
     1081 agtatggtgc tagggaggtt tctgtcgcaa ataagagaga agctgatcca gcggatatac
     1141 cgtggcattg agcccaccct tcccaattgg tttgccgtga ccaaaactcg taacggagca
     1201 ggggggggaa ataaagtcgt cgacgagtgc tatattccga actacctctt gcccaagacg
     1261 cagcccgaat tgcagtgggc ctggaccaac atggagcaat acctgtcagc gtgcctcaac
     1321 ttgaccgaaa gaaagagact cgtggcccag cacctgaccc atgtctcaca gacccaggaa
     1381 cagaataagg aaaaccaaaa cccaaatagc gacgcccccg tgatacggag caagacctca
     1441 gccaggtaca tggagctggt cgggtggctc gtggacaagg ggattacctc ggagaagcag
     1501 tggatccagg aggaccaggc ctcatacatc tccttcaatg cggcctccaa ctcgcggtcc
     1561 caaatcaagg ctgccttgga caatgcggga aagattatga gcctgactaa aaccgccccc
     1621 gactacctgg tgggccagca gcccgtggag gacatttcca gcaatcggat ttataaaatt
     1681 ttggaactaa acgggtacga tccccaatat gcggcttccg tctttctggg atgggccacg
     1741 aaaaagttcg gcaagaggaa caccatctgg ctgtttgggc ctgcaactac cgggaagacc
     1801 aacatcgcgg aggccatagc ccacactgtg cccttttacg gatgcgtgaa ttggactaac
     1861 gaaaatttcc cctttaatga ttgcgtggac aagatggtta tttggtggga ggaaggaaag
     1921 atgactgcga aagtggtgga atccgctaag gctatcttgg gggggtcgaa agttcgggtc
     1981 gaccagaagt gcaaatcgtc cgcgcagatt gaccccaccc ccgtgattgt gacgtcaaat
     2041 actaatatgt gtgcggtcat cgatggcaat agcaccactt tcgaacatca gcaacccctc
     2101 caggatcgta tgtttaagtt cgagttgact cggcggctgg accacgattt cggcaaagtg
     2161 acgaaacagg aggtgaagga cttctttaga tgggccaagg accacgtggt ggaggtcgag
     2221 cacgagtttt atgtgaagaa ggggggggcc aaaaagcgcc ctgcaccttc cgacgccgac
     2281 atttccgagc caaagagagt gcgtgagagt gtggcccaac cctccaccag tgatgccgag
     2341 gcctccatta attatgccga ccgctatcag aataaatgtt ctcgtcacgt gggcatgaat
     2401 ctgatgctgt ttccctgcag acaatgcgag agaatgaatc agaattcaaa tatctgcttc
     2461 actcacggac agaaagactg tttagagtgc tttcccgtgt cagaatctca acccgtttct
     2521 gtcgtcaaaa aggcgtatca gaaactgtgc tacattcatc atatcatggg aaaggtgcca
     2581 gacgcttgca ctgcctgcga tctggtcaat gtggatttgg atgactgcat ctttgaacaa
     2641 taagcggccg cactcgagat atctagaccc agctttcttg tacaaagtgg tgatcgcgtg
     2701 catgcgacgt catagctctc tccctatagt gagtcgtatt ataagctagg cactggccgt
     2761 cgttttacaa cgtcgtgact gggaaaactg ctagtcgaga atcaacctct ggattacaaa
     2821 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     2881 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     2941 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     3001 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     3061 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     3121 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3181 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     3241 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     3301 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     3361 cggatctccc tttgggccgc ctccccgcct gggtaccttt aagaccaatg acttacaagg
     3421 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3481 cccaaagaag acaagatctg ctttttgcct gtactgggtc tctctggtta gaccagatct
     3541 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3601 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     3661 tcagaccctt ttagtcagtg tggaaaatct ctagcaccca ggaggtagag gttgcagtga
     3721 gccaagatcg cgccactgca ttccagcctg ggcaagaaaa caagactgtc taaaataata
     3781 ataataagtt aagggtatta aatatattta tacatggagg tcataaaaat atatatattt
     3841 gggctgggcg cagtggctca cacctgcgcc cggccctttg ggaggccgag gcaggtggat
     3901 cacctgagtt tgggagttcc agaccagcct gaccaacatg gagaaacccc ttctctgtgt
     3961 atttttagta gattttattt tatgtgtatt ttattcacag gtatttctgg aaaactgaaa
     4021 ctgtttttcc tctactctga taccacaaga atcatcagca cagaggaaga cttctgtgat
     4081 caaatgtggt gggagaggga ggttttcacc agcacatgag cagtcagttc tgccgcagac
     4141 tcggcgggtg tccttcggtt cagttccaac accgcctgcc tggagagagg tcagaccaca
     4201 gggtgagggc tcagtcccca agacataaac acccaagaca taaacaccca acaggtccac
     4261 cccgcctgct gcccaggcag agccgattca ccaagacggg aattaggata gagaaagagt
     4321 aagtcacaca gagccggctg tgcgggagaa cggagttcta ttatgactca aatcagtctc
     4381 cccaagcatt cggggatcag agtttttaag gataacttag tgtgtagggg gccagtgagt
     4441 tggagatgaa agcgtaggga gtcgaaggtg tccttttgcg ccgagtcagt tcctgggtgg
     4501 gggccacaag atcggatgag ccagtttatc aatccggggg tgccagctga tccatggagt
     4561 gcagggtctg caaaatatct caagcactga ttgatcttag gttttacaat agtgatgtta
     4621 ccccaggaac aatttgggga aggtcagaat cttgtagcct gtagctgcat gactcctaaa
     4681 ccataatttc ttttttgttt tttttttttt atttttgaga cagggtctca ctctgtcacc
     4741 taggctggag tgcagtggtg caatcacagc tcactgcagc ctcaacgtcg taagctcaag
     4801 cgatcctccc acctcagcct gcctggtagc tgagactaca agcgacgccc cagttaattt
     4861 ttgtattttt ggtagaggca gcgttttgcc gtgtggccct ggctggtctc gaactcctgg
     4921 gctcaagtga tccagcctca gcctcccaaa gtgctgggac aaccggggcc agtcactgca
     4981 cctggcccta aaccataatt tctaatcttt tggctaattt gttagtccta caaaggcagt
     5041 ctagtcccca ggcaaaaagg gggtttgttt cgggaaaggg ctgttactgt ctttgtttca
     5101 aactataaac taagttcctc ctaaacttag ttcggcctac acccaggaat gaacaaggag
     5161 agcttggagg ttagaagcac gatggaattg gttaggtcag atctctttca ctgtctgagt
     5221 tataattttg caatggtggt tcaaagactg cccgcttctg acaccagtcg ctgcattaat
     5281 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5341 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5401 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5461 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5521 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5581 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5641 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5701 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5761 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5821 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     5881 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     5941 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6001 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6061 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6121 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6181 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6241 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6301 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6361 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6421 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     6481 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     6541 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     6601 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     6661 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     6721 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     6781 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     6841 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     6901 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     6961 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7021 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7081 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7141 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7201 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7261 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     7321 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7381 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7441 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     7501 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7561 ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     7621 cgctattacg ccaggggagg cagagattgc agtaagctga gatcgcagca ctgcactcca
     7681 gcctgggcga cagagtaaga ctctgtctca aaaataaaat aaataaatca atcagatatt
     7741 ccaatctttt cctttattta tttatttatt ttctattttg gaaacacagt ccttccttat
     7801 tccagaatta cacatatatt ctatttttct ttatatgctc cagttttttt tagaccttca
     7861 cctgaaatgt gtgtatcgag acctagaaaa acatggagca atcacaagta gcaatacagc
     7921 agctaacaat gctgcttgtg cctggctaga agcacaagag gaggaagagg tgggttttcc
     7981 agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     8041 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaaagaa gacaagatat
     8101 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     8161 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     8221 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     8281 cctgcatgga atggatgacc ctgagagaga agtgttagag tggaggtttg acagccgcct
     8341 agcatttcat cacgtggccc gagagctgca tccggagtat ctagatggag ttccgcgtta
     8401 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     8461 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     8521 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
     8581 ttgacgcaaa tgggcggtag gcgtgtactc tagatgctac atataagcag ctgctttttg
     8641 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     8701 ggaacccact gcttaagcct caataaagct tgccttgagt gctcaaagta gtgtgtgccc
     8761 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8821 tctctagcag tggcgcccga acagggactt gaaagcgaaa gtaaagccag aggagatctc
     8881 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     8941 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     9001 cggtattaag cgggggagaa ttagataaat gggaaaaaat tcggttaagg ccagggggaa
     9061 agaaacaata taaactaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     9121 ttaatcctgg ccttttagag acatcagaag gctgtagaca aatactggga cagctacaac
     9181 catcccttca gacaggatca gaagaactta gatcattata taatacaata gcagtcctct
     9241 attgtgtgca tcaaaggata gatgtaaaag acaccaagga agccttagat aagatagagg
     9301 aagagcaaaa caaaagtaag aaaaaggcac agcaagcagc agctgacaca ggaaacaaca
     9361 gccaggtcag ccaaaattac cctatagtgc agaacctcca ggggcaaatg gtacatcagg
     9421 ccatatcacc tagaacttta aatatgactc cgcccatccc gcccctaact ccgcccagtt
     9481 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9541 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9601 gcaaatgcat aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     9661 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     9721 ttttcgggtt tattacaggg acagcagaga tccacaattg gagaagtgaa ttatataaat
     9781 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     9841 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     9901 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     9961 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
    10021 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
    10081 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
    10141 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaataaca
    10201 tgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
    10261 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
    10321 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
    10381 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctgtagtga
    10441 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca atcccgaggg
    10501 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
    10561 ttcgattagt gaacggatct agttaactag atctagagtc
//
