LOCUS       sequence-377706-       16211 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Module for dCasEV2.1 activated expression of AtrD11, HarFAR and 
            ScATF1 plus gRNA-1DFR..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16211)
  AUTHORS   Kallam K, Moreno-Gimenez E, Mateos-Fernandez R, Tansley C, Gianoglio
            S, Orzaez D, Patron N
  TITLE     Tunable control of insect pheromone biosynthesis in Nicotiana 
            benthamiana.
  JOURNAL   Plant Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048.
  PUBMED    37032497
REFERENCE   2  (bases 1 to 16211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2023 Apr 9. doi: 10.1111/pbi.14048."
COMMENT     Sequence Label: GB3897
FEATURES             Location/Qualifiers
     source          1..16211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(677..1468)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2254..2278
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        2916..3260
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             3340..3726
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2
                     /note="binds to a specific stem-loop structure in the viral
                     RNA (Peabody, 1993)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             3733..3753
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3757..3777
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3781..3801
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3802..3951
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             3952..3972
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3976..3996
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3997..4353
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             4378..4947
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     terminator      4974..5226
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        6070..6414
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             6491..6511
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(6512..6535,6536..6538,6539..9022,9023..9028,9029..9094,
                     9095..9097,9098..10612)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(6512..6535,6536..6538,6539..9025,9026..9028,
                     9029..10612)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             6512..10612
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             10631..10651
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10655..10675
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10679..10699
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      10798..11050
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_RNA        11670..11745
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    11826..11850
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             13151..13780
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             14209..15282
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15348..15542
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(15778..15800)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15886..16026
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15941..15960
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(16041..16058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      661 catgcattct aggtgattat ttgccgacta ccttggtgat ctcgcctttc acgtagtgga
      721 caaattcttc caactgatct gcgcgcgagg ccaagcgatc ttcttcttgt ccaagataag
      781 cctgtctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc attgcccagt
      841 cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa atgcgggaca
      901 acgtaagcac tacatttcgc tcatcaccag cccagtcggg cggcgagttc catagcgtta
      961 aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag agttcctccg
     1021 ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag atagccagat
     1081 caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc tgccattctc
     1141 caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg tgcacaacaa
     1201 tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa gtttccaaaa
     1261 ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc gtaaccagca
     1321 aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac aaatgtacgg
     1381 ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat agttgagtcg
     1441 atacttcggc gatcaccgct tccctcataa tgtttaactt tgttttaggg cgactgccct
     1501 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
     1561 gctgcttgga tgcccgaggc atagactgta ccccaaaaaa acagtcataa caagccatga
     1621 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     1681 agcgcatacg ctacttgcat tacagcttac gaaccgaaca ggcttatgtc cactgggttc
     1741 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg
     1801 aggcatttct gtcctggctg gaacagaact tattatttcc ttcctctttt ctacagtatt
     1861 taaagatacc ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat
     1921 tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata
     1981 acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc
     2041 atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt
     2101 agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc
     2161 tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc
     2221 gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg gtgtaaacat
     2281 aacgatatcg gtctcagtca ggagactaga gccaagctga tctcctttgc cccggagatc
     2341 accatggacg actttctcta tctctacgat ctaggaagaa agttcgacgg agaaggtgac
     2401 gataccatgt tcaccaccga taatgagaag attagcctct tcaatttcag aaagaatgct
     2461 gacccacaga tggttagaga ggcctacgcg gcaggtctga tcaagacgat ctacccgagt
     2521 aataatctcc aggagatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc
     2581 aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt
     2641 ccagtatgga cgattcaagg cttgcttcat aaaccaaggc aagtaataga gattggagtc
     2701 tctaagaaag tagttcctac tgaatcaaag gccatggagt caaaaattca gatcgaggat
     2761 ctaacagaac tcgccgtgaa gactggcgaa cagttcatac agagtctttt acgactcaat
     2821 gacaagaaga aaatcttcgt caacatggtg gagcacgaca ctctcgtcta ctccaagaat
     2881 atcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata
     2941 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta
     3001 gaaaaggaag gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa
     3061 gatgcccctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     3121 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     3181 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     3241 tcatttcatt tggagaggac tccggtattt ttacaacaat taccacaaca aaacaaacaa
     3301 caaacaacat tacaatttac tattctagtc gaaatgatgg cttctaattt tactcaattt
     3361 gttcttgttg ataatggtgg tactggtgat gttactgttg ctccttctaa ttttgctaat
     3421 ggtgttgctg aatggatttc ttctaattct agatctcaag cttataaggt tacttgttct
     3481 gttagacaat cttctgctca aaatagaaag tatactatta aggttgaagt tcctaaggtt
     3541 gctactcaaa ctgttggtgg tgttgaactt cctgttgctg cttggagatc ttatcttaat
     3601 atggaactta ctattcctat ttttgctact aattctgatt gtgaacttat tgttaaggct
     3661 atgcaaggtc ttcttaagga tggtaatcct attccttctg ctattgctgc taattctggt
     3721 atttattcgg acccaaaaaa gaagcgtaag gtcgatccca agaagaagag aaaggtagat
     3781 cctaagaaga agagaaaggt agacgcattg gatgacttcg acttagacat gcttgggtca
     3841 gatgctttgg acgactttga cctcgacatg ttgggttctg acgcgctaga tgacttcgac
     3901 ttggacatgt tgggctccga cgctttggac gactttgatc tggacatgtt accgaaaaag
     3961 aaacgcaaag ttgatcctaa gaagaagaga aaggtaccta cacaggccgg cgagggcaca
     4021 ctgtctgaag ctctgctgca gctgcagttc gacgacgagg atctgggagc cctgctggga
     4081 aacagcaccg atcctgccgt gttcaccgac ctggccagcg tggacaacag cgagttccag
     4141 cagctgctga accagggcat ccctgtggcc cctcacacca ccgagcccat gctgatggaa
     4201 taccccgagg ccatcacccg gctcgtgaca ggcgctcaga ggcctcctga tccagctcct
     4261 gcccctctgg gagcaccagg cctgcctaat ggactgctgt ctggcgacga ggacttcagc
     4321 tctatcgccg atatggattt ctcagccttg ctgattaact ctagaagttc cggatctcgg
     4381 gattccaggg aagggatgtt tttgccgaag cctgaggccg gctccgctat tagtgacgtg
     4441 tttgagggcc gcgaggtgtg ccagccaaaa cgaatccggc catttcatcc tccaggaagt
     4501 ccatgggcca accgcccact ccccgccagc ctcgcaccaa caccaaccgg tccagtacat
     4561 gagccagtcg ggtcactgac cccggcacca gtccctcagc cactggatcc agcgcccgca
     4621 gtgactcccg aggccagtca cctgttggag gatcccgatg aagaaacgag ccaggctgtc
     4681 aaagcccttc gggagatggc cgatactgtg attccccaga aggaagaggc tgcaatctgt
     4741 ggccaaatgg acctttccca tccgccccca aggggccatc tggatgagct gacaaccaca
     4801 cttgagtcca tgaccgagga tctgaacctg gactcacccc tgaccccgga attgaacgag
     4861 attctggata ccttcctgaa cgacgagtgc ctcttgcatg ccatgcatat cagcacagga
     4921 ctgtccatct tcgacacatc tctgttttga gcttggaatg gatcttcgat cccgatcgtt
     4981 caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta
     5041 tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt
     5101 tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag
     5161 aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac
     5221 tagatcggga attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt
     5281 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     5341 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5401 atgagacaat aaccctgata aatgcttcaa taatgggacc gactcgcgct gtcaggagac
     5461 tagagccaag ctgatctcct ttgccccgga gatcaccatg gacgactttc tctatctcta
     5521 cgatctagga agaaagttcg acggagaagg tgacgatacc atgttcacca ccgataatga
     5581 gaagattagc ctcttcaatt tcagaaagaa tgctgaccca cagatggtta gagaggccta
     5641 cgcggcaggt ctgatcaaga cgatctaccc gagtaataat ctccaggaga tcaaatacct
     5701 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     5761 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     5821 tcataaacca aggcaagtaa tagagattgg agtctctaag aaagtagttc ctactgaatc
     5881 aaaggccatg gagtcaaaaa ttcagatcga ggatctaaca gaactcgccg tgaagactgg
     5941 cgaacagttc atacagagtc ttttacgact caatgacaag aagaaaatct tcgtcaacat
     6001 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
     6061 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
     6121 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
     6181 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc cctgccgaca gtggtcccaa
     6241 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     6301 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     6361 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggactccggt
     6421 atttttacaa caattaccac aacaaaacaa acaacaaaca acattacaat ttactattct
     6481 agtcgaaatg cccaagaaga agaggaaggt ggacaagaag tactccattg ggctcgctat
     6541 cggcacaaac agcgtcggct gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa
     6601 attcaaagtt ctgggcaata ccgatcgcca cagcataaag aagaacctca ttggcgccct
     6661 cctgttcgac tccggggaaa cggccgaagc cacgcggctc aaaagaacag cacggcgcag
     6721 atatacccgc agaaagaatc ggatctgcta cctgcaggag atctttagta atgagatggc
     6781 taaggtggat gactctttct tccataggct ggaggagtcc tttttggtgg aggaggataa
     6841 aaagcacgag cgccacccaa tctttggcaa tatcgtggac gaggtggcgt accatgaaaa
     6901 gtacccaacc atatatcatc tgaggaagaa gcttgtagac agtactgata aggctgactt
     6961 gcggttgatc tatctcgcgc tggcgcatat gatcaaattt cggggacact tcctcatcga
     7021 gggggacctg aacccagaca acagcgatgt cgacaaactc tttatccaac tggttcagac
     7081 ttacaatcag cttttcgaag agaacccgat caacgcatcc ggagttgacg ccaaagcaat
     7141 cctgagcgct aggctgtcca aatcccggcg gctcgaaaac ctcatcgcac agctccctgg
     7201 ggagaagaag aacggcctgt ttggtaatct tatcgccctg tcactcgggc tgacccccaa
     7261 ctttaaatct aacttcgacc tggccgaaga tgccaagctt caactgagca aagacaccta
     7321 cgatgatgat ctcgacaatc tgctggccca gatcggcgac cagtacgcag accttttttt
     7381 ggcggcaaag aacctgtcag acgccattct gctgagtgat attctgcgag tgaacacgga
     7441 gatcaccaaa gctccgctga gcgctagtat gatcaagcgc tatgatgagc accaccaaga
     7501 cttgactttg ctgaaggccc ttgtcagaca gcaactgcct gagaagtaca aggaaatttt
     7561 cttcgatcag tctaaaaatg gctacgccgg atacattgac ggcggagcaa gccaggagga
     7621 attttacaaa tttattaagc ccatcttgga aaaaatggac ggcaccgagg agctgctggt
     7681 aaagcttaac agagaagatc tgttgcgcaa acagcgcact ttcgacaatg gaagcatccc
     7741 ccaccagatt cacctgggcg aactgcacgc tatcctcagg cggcaagagg atttctaccc
     7801 ctttttgaaa gataacaggg aaaagattga gaaaatcctc acatttcgga taccctacta
     7861 tgtaggcccc ctcgcccggg gaaattccag attcgcgtgg atgactcgca aatcagaaga
     7921 gactatcact ccctggaact tcgaggaagt cgtggataag ggggcctctg cccagtcctt
     7981 catcgaaagg atgactaact ttgataaaaa tctgcctaac gaaaaggtgc ttcctaaaca
     8041 ctctctgctg tacgagtact tcacagttta taacgagctc accaaggtca aatacgtcac
     8101 agaagggatg agaaagccag cattcctgtc tggagagcag aagaaagcta tcgtggacct
     8161 cctcttcaag acgaaccgga aagttaccgt gaaacagctc aaagaagatt atttcaaaaa
     8221 gattgaatgt ttcgactctg ttgaaatcag cggagtggag gatcgcttca acgcatccct
     8281 gggaacgtat cacgatctcc tgaaaatcat taaagacaag gacttcctgg acaatgagga
     8341 gaacgaggac attcttgagg acattgtcct cacccttacg ttgtttgaag atagggagat
     8401 gattgaagaa cgcttgaaaa cttacgctca tctcttcgac gacaaagtca tgaaacagct
     8461 caagaggcgc cgatatacag gatgggggcg gctgtcaaga aaactgatca atgggatccg
     8521 agacaagcag agtggaaaga caatcctgga ttttcttaag tccgatggat ttgccaaccg
     8581 gaacttcatg cagttgatcc atgatgactc tctcaccttt aaggaggaca tccagaaagc
     8641 acaagtttct ggccaggggg acagtctcca cgagcacatc gctaatcttg caggtagccc
     8701 agctatcaaa aagggaatac tgcagaccgt taaggtcgtg gatgaactcg tcaaagtaat
     8761 gggaaggcat aagcccgaga atatcgttat cgagatggcc cgagagaacc aaactaccca
     8821 gaagggacag aagaacagta gggaaaggat gaagaggatt gaagagggta taaaagaact
     8881 ggggtcccaa atccttaagg aacacccagt tgaaaacacc cagcttcaga atgagaagct
     8941 ctacctgtac tacctgcaga acggcaggga catgtacgtg gatcaggaac tggacatcaa
     9001 tcggctctcc gactacgacg tggatgccat cgtgccccag tcttttctca aagatgattc
     9061 tattgataat aaagtgttga caagatccga taaaaataga gggaagagtg ataacgtccc
     9121 ctcagaagaa gttgtcaaga aaatgaaaaa ttattggcgg cagctgctga acgccaaact
     9181 gatcacacaa cggaagttcg ataatctgac taaggctgaa cgaggtggcc tgtctgagtt
     9241 ggataaagcc ggcttcatca aaaggcagct tgttgagaca cgccagatca ccaagcacgt
     9301 ggcccaaatt ctcgattcac gcatgaacac caagtacgat gaaaatgaca aactgattcg
     9361 agaggtgaaa gttattactc tgaagtctaa gctggtttca gatttcagaa aggactttca
     9421 gttttataag gtgagagaga tcaacaatta ccaccatgcg catgatgcct acctgaatgc
     9481 agtggtaggc actgcactta tcaaaaaata tcccaagctt gaatctgaat ttgtttacgg
     9541 agactataaa gtgtacgatg ttaggaaaat gatcgcaaag tctgagcagg aaataggcaa
     9601 ggccaccgct aagtacttct tttacagcaa tattatgaat tttttcaaga ccgagattac
     9661 actggccaat ggagagattc ggaagcgacc acttatcgaa acaaacggag aaacaggaga
     9721 aatcgtgtgg gacaagggta gggatttcgc gacagtccgg aaggtcctgt ccatgccgca
     9781 ggtgaacatc gttaaaaaga ccgaagtaca gaccggaggc ttctccaagg aaagtatcct
     9841 cccgaaaagg aacagcgaca agctgatcgc acgcaaaaaa gattgggacc ccaagaaata
     9901 cggcggattc gattctccta cagtcgctta cagtgtactg gttgtggcca aagtggagaa
     9961 agggaagtct aaaaaactca aaagcgtcaa ggaactgctg ggcatcacaa tcatggagcg
    10021 atcaagcttc gaaaaaaacc ccatcgactt tctcgaggcg aaaggatata aagaggtcaa
    10081 aaaagacctc atcattaagc ttcccaagta ctctctcttt gagcttgaaa acggccggaa
    10141 acgaatgctc gctagtgcgg gcgagctgca gaaaggtaac gagctggcac tgccctctaa
    10201 atacgttaat ttcttgtatc tggccagcca ctatgaaaag ctcaaaggat ctcccgaaga
    10261 taatgagcag aagcagctgt tcgtggaaca acacaaacac taccttgatg agatcatcga
    10321 gcaaataagc gaattctcca aaagagtgat cctcgccgac gctaacctcg ataaggtgct
    10381 ttctgcttac aataagcaca gggataagcc catcagggag caggcagaaa acattatcca
    10441 cttgtttact ctgaccaact tgggcgcgcc tgcagccttc aagtacttcg acaccaccat
    10501 agacagaaag cggtacacct ctacaaagga ggtcctggac gccacactga ttcatcagtc
    10561 aattacgggg ctctatgaaa caagaatcga cctctctcag ctcggtggag acagcagggc
    10621 tgattcggat cctaaaaaga agcgaaaggt agacccgaag aaaaagagga aggtagatcc
    10681 aaagaagaag cgcaaggtag aagtgtttga atttgaatat ctggatgata aagtgctgga
    10741 agaactgctg gatagcgaag aacgcaaacg ctgagcttgg aatggatctt cgatcccgat
    10801 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgacg
    10861 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
    10921 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
    10981 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
    11041 ttactagatc gggaattgcc aagctaattc ttgaagacga aagggcctcg tgatacgcct
    11101 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
    11161 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
    11221 gctcatgaga caataaccct gataaatgct tcaataatgg gaccgactcg cgctgtcagg
    11281 agtctcaatg gtaactttac tctttattta accatacatt tttttttatt tttttcactt
    11341 tgttcttcat ccactattgt tctttgttca tcttgaacaa aagctccctc cttctttgtt
    11401 cttcatccac cattgttctt catcaatcat ttcgctgtca ggagcatctt cattcttaag
    11461 atatgaagat aatcttcaaa aggcccctgg gaatctgaaa gaagagaagc aggcccattt
    11521 atatgggaaa gaacaatagt atttcttata taggcccatt taagttgaaa acaatcttca
    11581 aaagtcccac atcgcttaga taagaaaacg aagctgagtt tatatacagc tagagtcgaa
    11641 gtagtgattg actggttggt gagagaagag ttttagagct agaaatagca agttaaaata
    11701 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcgggag cacatgagga
    11761 atcacccatg tgcgactccc acagtcactg gggagtcttc ccttttttcg cttgagaccg
    11821 atatctgaca ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat
    11881 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa
    11941 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca
    12001 gtcggcttct gacgttcagt gcagccgtca tctgaaaacg acatgtcgca caagtcctaa
    12061 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt
    12121 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc
    12181 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa
    12241 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc
    12301 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg
    12361 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag
    12421 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc
    12481 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc
    12541 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt
    12601 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag
    12661 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac
    12721 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc
    12781 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag
    12841 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga
    12901 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac ctctcaaccg
    12961 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca
    13021 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa
    13081 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca
    13141 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac
    13201 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga
    13261 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac
    13321 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga
    13381 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc
    13441 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga
    13501 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt
    13561 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc
    13621 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac
    13681 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt
    13741 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa
    13801 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct
    13861 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg
    13921 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag
    13981 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag
    14041 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc
    14101 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgc
    14161 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gagcggtcgc
    14221 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg
    14281 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg
    14341 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg
    14401 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc
    14461 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg
    14521 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag
    14581 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg
    14641 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc
    14701 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga
    14761 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg
    14821 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg
    14881 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag
    14941 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt
    15001 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc
    15061 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc
    15121 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac
    15181 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc
    15241 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta
    15301 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggac tctttcctgt ggatagcacg
    15361 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag
    15421 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat
    15481 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca
    15541 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg
    15601 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg
    15661 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac
    15721 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
    15781 ctgacacatg cagctcccgg tgacggtcac agcttgtctg taagcggatg ccgggagcag
    15841 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
    15901 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta
    15961 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
    16021 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    16081 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    16141 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    16201 ttgctggcgt t
//
