LOCUS       sequence-37786-e         486 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 486)
  TITLE     mammalian homologous recombination reporter genes
REFERENCE   2  (bases 1 to 486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGCGFP-puro
FEATURES             Location/Qualifiers
     source          1..486
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     94..113
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     353..374
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      416..425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
       61 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      121 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
      181 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
      241 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
      301 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
      361 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
      421 gtggcgaccg gtggatccac gccgggcttg gcgaagtgcc tcagctccag gtgcctcctc
      481 aggttc
//
