LOCUS       sequence-377867-        4219 bp    DNA     circular SYN 15-APR-2025
DEFINITION  Yeast gene induction with meiotic promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Dawson, D.
  TITLE     Dawson Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFA6a-kanMX6-pSPO11
FEATURES             Location/Qualifiers
     source          1..4219
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            387..1743
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        387..730
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             731..1540
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(798..817)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      1546..1743
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1847..1866)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1848..1866)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1953..1970)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2124..2712)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2204..2223)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2883..3674,3675..3743))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3506..3525
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3744..3848)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3916..3934
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(3972..3994)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4094..4113
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4194..4212
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     4194..4211
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cgccagctga agcttcgtac gccattttga gatccgggtt ttgagaaggt gaacttagta
       61 aatcgtcaaa tcttaagggt gaagacaaat atgtgaggtt atttgaatga tatttagttg
      121 gatataatca ctaaataaag tagattttcc ttgggtagta gactttcttt tacgttattc
      181 cttttataat tttaatttta gttattgatt tcaatgaaac aaaaggaaac tgacggcttc
      241 ttaaaataaa tatatttaac atgaaacatt aaaaaaaaaa ttgtcactac acagagagaa
      301 aataaataaa tataaagaat cacataacta tgctgaaaaa atgaagtgtt tagcttgcct
      361 cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc
      421 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
      481 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
      541 acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
      601 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
      661 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
      721 ataaacaacc atgggtaagg aaaagactca cgtttcgagg ccgcgattaa attccaacat
      781 ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac
      841 aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg
      901 tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat
      961 gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac
     1021 tgcgatcccc ggcaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa
     1081 tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg
     1141 tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg
     1201 tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg
     1261 gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt
     1321 ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg
     1381 agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt
     1441 ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa
     1501 taaattgcag tttcatttga tgctcgatga gtttttctaa tcagtactga caataaaaag
     1561 attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt
     1621 taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg
     1681 aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata
     1741 ctgctgtcga ttcgatacta acgccgccat ccagtttaaa cgagctcgaa ttcatcgatg
     1801 atatcagatc cactagtggc ctatgcggcc gcggatctgc cggtctccct atagtgagtc
     1861 gtattaattt cgataagcca ggttaacctg cattaatgaa tcggccaacg cgcggggaga
     1921 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     1981 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2041 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2101 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2161 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2221 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     2281 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     2341 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     2401 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     2461 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     2521 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     2581 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     2641 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     2701 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     2761 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     2821 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     2881 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     2941 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3001 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3061 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3121 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3181 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3241 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     3301 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     3361 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     3421 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     3481 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     3541 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     3601 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     3661 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     3721 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     3781 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     3841 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     3901 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     3961 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     4021 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     4081 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat ggacatattg
     4141 tcgttagaac gcggctacaa ttaatacata accttatgta tcatacacat acgatttagg
     4201 tgacactata gaacgcggc
//
