LOCUS       sequence-377888-         976 bp    DNA     circular SYN 15-APR-2025
DEFINITION  E. coli - Eggerthella lenta shuttle plasmid (KanR), Pcadh catechin 
            dehydroxylase promoter-lacZ with transcriptional regulator CadR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Dong X, Guthrie BGH, Alexander M, Noecker C, Ramirez L, Glasser NR, 
            Turnbaugh PJ, Balskus EP
  TITLE     Genetic manipulation of the human gut bacterium Eggerthella lenta 
            reveals a widespread family of transcriptional regulators.
  JOURNAL   Nat Commun. 2022 Dec 9;13(1):7624. doi: 10.1038/s41467-022-33576-3.
  PUBMED    36494336
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-9"; volume: "13(1):7624. doi"; pages: " 
            10.1038/s41467-022-33576-3"
COMMENT     Sequence Label: pXD70LacZ8-CadR-Pcadh-LacZ
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     90..112
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     104..121
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     105..121
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gnnatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
       61 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      121 tgaatccgta atcatggtca tagctcctcc tctgccggtt cccttttcac ccgcactcca
      181 acgagcgggc cggaccaagc gtccgtgcga accatgatgc agatcgagcg cgagttgagc
      241 accccaccgc tgatgcgaac tctgttcaac caggtcagaa accctactat cgagctcatg
      301 aaagacctac caccgagagt ggtatttcgc catccgcccg ccgccgcggt atcggcgaat
      361 tgctatactg acgacaatgc tcgaacgagc cttatcgcca tggggatgtg agtaaaaggg
      421 ggtccgcatg acagacgaac gaggatcgtc gccgcttgcg ctggcgattc gcgaggtgcc
      481 gggaatcgcg atcgcttcga tcggcttcgg atgcgcgctg ctcgccatcg aaggcgaccg
      541 cctctcgacg atcagaaccg tcaatatcag cggcgactac cacgccgtcg cgtcgctcgt
      601 atcgttcctt ctcctgatca tgctggctct tgcgtatcgc aagctggacg cgcccaccca
      661 cgttcccgcc gcgatcgtca tcggcgcang agcgttgatg tcggcttcca tgttcgtcgt
      721 cttgcagcaa agcgtatggg aggacaaccc catcgtatcc tccatcgcgc gcatcgtatt
      781 cgggcttgga gagggcgtca tcctgtttct ctggatgaaa gcgctctttc cctanggggc
      841 gcgcgcctgc gccgccgtgc tgggcctggg aacgatcgtc gttgcggcaa tgaacagcct
      901 catcgcgttt ctgaanngga tgccgcttac ggcacggtat cgctgctccc atcgtgtcga
      961 nggngctgct ctgcta
//
