LOCUS       sequence-378-exp        1879 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1879)
  TITLE     TAP
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: neo/TAP Myc
FEATURES             Location/Qualifiers
     source          1..1879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..795
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(55..74)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     665..684
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             1319..1492
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             1493..1666
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             1736..1756
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1757..1786
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1802..1879
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
       61 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
      121 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
      181 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
      241 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
      301 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
      361 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
      421 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
      481 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
      541 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
      601 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
      661 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
      721 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
      781 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
      841 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
      901 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
      961 cccaccccaa ttcgagctcg gtacccctag aaagtgtgac aacgtcgcac catgtgtagg
     1021 ttttcattta tgttctttct ttctttcttt tgtgaatttg ttttctgtct caaatgtttt
     1081 taattcgctt gggacctatg tttttcttgt ttttttgctc accctttgtg taggaggcac
     1141 cctgtcacgt ctgtggttgc gtgtatgcct tccttcccct tattcgcttc ttcctgtcgt
     1201 gtcacacctc tttctccctc tccctttccg ccttttcttt caatcttgtt ttctcgacca
     1261 gccctactag aggagnaaga atagtaaccc tttcatcaaa tagtccaaac gaattatggt
     1321 ggacaacaaa ttcaacaaag aacaacaaaa cgcgttctat gagatcttac atttacctaa
     1381 cttaaacgaa gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag
     1441 cgctaacctt ttagcagaag ctaaaaagct aaatgatgct caggcgccga aagtagacaa
     1501 caaattcaac aaagaacaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa
     1561 cgaagaacaa cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa
     1621 ccttttagca gaagctaaaa agctaaatgg tgctcaggcg ccgaaagtag acgcgaattc
     1681 cgcggggaag tcaaccggtc tcaaaaccgc ggctcttgcg caacacgatg aagccgagaa
     1741 tttgtatttt cagggtgaac agaaacttat ttcggaggag gacttagttg agcaaaagtt
     1801 aaagagaaga tggaaaaaga atttcatagc cgtctcagca gccaaccgct ttaagaaaat
     1861 ctcatcctcc ggggcactt
//
